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Advances in Microbiology 2019年第1期9卷 21-37页

作者： Shijie Zheng Shude Yang Xianhao Cheng Tolgor Bau Yu Li Rui Zhang Haiying Bao Engineering Research Center Chinese Ministry of Education for Edible and Medicinal Fungi Jilin Agricultural University Changchun China Shandong Key lab of Edible mushroom Technology Ludong University Yantai China

The interaction between dehydroeburicoic acid (DeEA), a triterpene purified from medicinal fungi and the major transport protein, human serum albumin (HSA), were systematically studied by fluorescence spectroscopy, synchronous fluorescence spectroscopy, three-dimensional fluorescence spectroscopy and molecular docking approach under simulated physiological conditions. The intrinsic fluorescence of HSA was quenched through the combination of static and dynamic quenching mechanism. DeEA cannot be stored and carried by HSA in the body at higher temperature. The hydrogen bonding, hydrophobic force and van der Waals force were major acting forces. The site II was the major binding site. The energy transfer could occur with high probability and the binding distance was 3.29 nm. The binding process slightly changed the conformation and microenvironment of HSA. The DeEA molecule entered the hydrophobic cleft of HSA and formed the hydrogen bonding with Glu-492 and Lys-545.

关键词： Human Serum Albumin Dehydroeburicoic Acid Interaction Fluorescence Molecular Docking

Innovative Consortia of Micro and Macro Fungal Systems: Cellulolytic Enzyme Production from Groundnut Shell Biomass and Supportive Structural Analysis

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Journal of Sustainable Bioenergy Systems 2018年第3期8卷 47-66页

作者： Sai Praneeth Thota Pradeep Kumar Badiya Yadhu Nadh Guragain Praveen Venkata Vadlani Meera Pandey Rajesh Babu Dandamudi Sai Sathish Ramamurthy Siva Kumar Belliraj Department of Chemistry Sri Sathya Sai Institute of Higher Learning Prasanthi Nilayam India Bioprocessing and Renewable Energy laboratory Departments of Grain Science and Industry & Chemical Engineering Kansas State University Manhattan KS USA mushroom lab Indian Institute Horticulture Research Bangalore India

Renewable biomass-derived fuels are essential to meet the blend mandates and the sustainability goals. In our first-to-date study, we investigated individual and synergistic consortia of fungal cultures comprising Pycnoporous sanguineus (PS) in combination with Aspergillus oryzae (AO) and Trichoderma harzianum (TH) for production of cellulolytic enzymes using groundnut shell under solid state and submerged liquid fermentation conditions. The innovative consortia closely align with the microbial ecosystems found in nature;consequently, we anticipate a potent and effective cellulolytic enzyme system, which maximises the breakdown of biomass polymers to sugars. Under ternary combination of cultures, cellulase production was highest at 123.0 ± 1 FPU/gds;β-glucosidase production at 875.6 ± 26.4 IU/g dry substrate (gds);and CMCase at 474.95 ± 45.5 IU/gds. β-glucosidase production was highest on the 2nd day at 987.03 ± 64.2 IU/gds while CMCase peaked at 514.97 ± 21.4 IU/gds on 2nd day for PS, maximum cellulase enzyme production was observed on the 6th day at 192.2 ± 0.96 FPU/gds (AO + PS). Present work showed that synergistic combination of fungal cultures for releasing balanced enzyme activities that can efficiently saccharify biomass, such as groundnut shell to sugars, which can subsequently be fermented to various bioproducts and biomaterial monomers. Elaborate characterization studies of enzyme treated groundnut shell revealed prominent physicochemical changes in the hydrolysate, which indicates the changes in biomass are due to the enzymatic action and the growth effects of the consortia;thereby, leading to promising applications for the microbial fortified biomass.

关键词： Mixed Cultures Fermentation Enzymatic Hydrolysis Groundnut Shell Biomass Characterization

Probing the Molecular Interaction between Verbascoside and Human Serum Albumin by Fluorescence Spectroscopy and Molecular Docking

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Probing the Molecular Interaction between Verbascoside and H...

Enhanced Compliant Adhesive Design and Fabrication with Dual-Level Hierarchical Structure

多彩菌物美丽中国——中国菌物学会2019年学术年会

作者： Shijie Zheng Shude Yang Rui Zhang Xianhao Cheng Haiying Bao Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi Jilin Agricultural University ShanDong Key lab of Edible mushroom Technology Ludong University

The mechanism and conformational changes of the interaction between verbascoside（VB） and human serum albumin（HSA） were systematically studied by fluorescence spectroscopy, synchronous fluorescence spectroscopy, three-dimensional fluorescence spectroscopy, and molecular docking. The intrinsic fluorescence of HSA was quenched by VB through the combination of static and dynamic quenching mechanism. According to the Scatchard equation, the binding constant and the number of binding site were evaluated. The thermodynamic parameters showed that the van der Waals forces and the hydrogen bonding were dominant in the binding process.The displacement experiments with site markers indicated that VB bound to HSA at site II（subdomain IIIA）. The synchronous fluorescence spectroscopy and three-dimensional fluorescence spectroscopy results suggested that the interaction between VB and HSA changed the polarity and microenvironment around tyrosine residues. The interaction between VB and the amino acid residues of HSA was shown by molecular docking approach, and the theoretical results were consistent with experimental results.

关键词： verbascoside interaction human serum albumin fluorescence molecular docking

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Journal of Bionic Engineering 2010年第3期7卷 228-234页

作者： Dan Sameoto Carlo Menon Department of Mechanical Engineering University of Alberta MENRVA lab School of Engineering ScienceSimon Fraser University

Synthetic dry adhesives inspired by the nano-and micro-scale hairs found on the feet of geckos and some spiders have beendeveloped for almost a decade. Elastomeric single level micro-scale mushroom shaped fibres are currently able to function evenbetter than natural dry adhesives on smooth surfaces under normal loading. However, the adhesion of these single level syntheticdry adhesives on rough surfaces is still not optimal because of the reduced contact surface area. In nature, contact area ismaximized by hierarchically structuring different scales of fibres capable of conforming surface roughness. In this paper, weadapt the nature’s solution arid propose a novel dual-level hierarchical adhesive design using Polydimethylsiloxane (PDMS),which is tested under peel loading at different orientations. A negative macro-scale mold is manufactured by using a laser cutterto define holes in a Poly(methyl methacrylate) (PMMA) plate. After casting PDMS macro-scale fibres by using the obtainedPMMA mold, a previously prepared micro-fibre adhesive is bonded to the macro-scale fibre substrate. Once the bondingpolymer is cured, the micro-fibre adhesive is cut to form macro scale mushroom caps. Each macro-fibre of the resulting hierarchicaladhesive is able to conform to loads applied in different directions. The dual-level structure enhances the peel strengthon smooth surfaces compared to a single-level dry adhesive, but also weakens the shear strength of the adhesive for a given areain contact. The adhesive appears to be very performance sensitive to the specific size of the fibre tips, and experiments indicatethat designing hierarchical structures is not as simple as placing multiple scales of fibres on top of one another, but can requiresignificant design optimization to enhance the contact mechanics and adhesion strength.

关键词： biomimetics dry adhesive hierarchical dual-level adhesive silicone polymer

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The carboxypeptidase D homolog silver regulates memory formation via insulin pathway in Drosophila

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Protein & Cell 2016年第8期7卷 606-610页

作者： Lu, Binyan Zhao, Yi Zhao, Jie Yao, Xiaoyang Shuai, Yichun Ma, Weiwei Zhong, Yi Tsinghua Univ McGovern Inst Brain Res Tsinghua Peking Ctr Life Sci Minist EducKey Lab Prot SciSch Life Sci Beijing 100084 Peoples R China Chinese Acad Sci Inst Bot State Key Lab Systemat & Evolutionary Bot Beijing 100093 Peoples R China

Dear Editor, What is the molecular basis of memory formation？ Many genes have been implicated in this process, including those involved in neural cell adhesion, mRNA transport, translation control, and cAMP-PKA signa... 详细信息

关键词： Memory Formation mushroom Body Neurosecretory Cell Enhancer Trap Insulin Pathway

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淡水真菌三个中国新记录种

菌物学报 2011年第1期30卷 123-127页

作者：陈燕李秀岚蔡磊金静张剑锋 Hyde D Kevin 路炳声青岛农业大学农学与植物保护学院青岛266109 青岛农业大学化学与药学院青岛266109 中国科学院微生物研究所北京100110 mushroom Research Foundation Thailand

在进行我国淡水真菌多样性的研究中,发现3个中国新记录种:墨鱼状假黑囊壳Pseudomassaria sepincoliformis,二隔四壳座菌Quaternaria dissepta和萨尼亚小镰孢Fusariella sarniensis。文中对每个种进行了形态描述及讨论,标本保存于青岛农... 详细信息

在进行我国淡水真菌多样性的研究中,发现3个中国新记录种:墨鱼状假黑囊壳Pseudomassaria sepincoliformis,二隔四壳座菌Quaternaria dissepta和萨尼亚小镰孢Fusariella sarniensis。文中对每个种进行了形态描述及讨论,标本保存于青岛农业大学菌物标本室。

关键词：中国新记录种真菌多样性子囊菌海南甘肃

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A Simplified Computational Model of mushroom Body for Tethered Bees' Abdominal Swing Behavior Induced by Optic Flow

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Chinese Journal of Electronics 2021年第2期30卷 296-302页

作者： ZHENG Nenggan MA Qian WANG Xuefei ZHAO Lei GONG Zhefeng Qiushi Academy for Advanced Studies Zhejiang University College of Computer Science and Technology Zhejiang University Department of Neurobiology Zhejiang University School of Medicine Zhejiang lab

Understanding sensorimotor neural circuits plays an important role in the study of behavioral mechanisms. By virtue of a relatively simple brain structure and sophisticated locomotion behaviors, insects are selected as comparative research subjects to discover the basic principles of neural science. Specific abdominal swing behaviors of tethered bees induced by optomotor response are realized. To model functionality of mushroom body in the optic-flow induced swing behaviors, a simplified 3-layer Spiking neural network(SNN) is proposed. Spike response model is used as the single neuron model in the proposed SNN, which is trained by supervised learning method. The computational model can accurately simulate and predict the bees’ abdominal swing behaviors exhibiting ipsilateral direction and proportional frequencies with optic flow stimulus.

关键词： Computational model Optomotor response Optic flow stimuli Abdominal swing Spiking neural network

Juvenile hormone receptor Met regulates sleep and neuronal morphology via glial-neuronal crosstalk

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Journal of Genetics and Genomics 2021年第8期48卷 706-715页

作者： Binbin Wu Lei He Yutong Xiao Juan Du Xiaoxiao Wang Zhangwu Zhao Department of Entomology and MOA Key lab of Pest Monitoring and Green Management College of Plant ProtectionChina Agricultural UniversityBeijing 100193China College of Life Sciences Hebei UniversityBaoding 071002China

Juvenile hormone(JH)is one of the most important hormones in insects since it is essential for insect development.The mechanism by which JH affects the central nervous system still remains a mystery.In this study,we demonstrate that one of the JH receptors,Methoprene-tolerant(Met),is important for the control of neurite development and sleep behavior in Drosophila.With the identification of Met-expressing glial cells,the mechanism that Met negatively controls the mushroom body(MB)b lobes fusion and positively maintains pigment-dispersing factor s LNvs projection pruning has been established.Furthermore,despite the developmental effects,Met can also maintain nighttime sleep in a developmentindependent manner through the a/b lobe of MB.Combining analyses of neuronal morphology and entomological behavior,this study advances our understanding of how the JH receptor regulates the nervous system.

关键词： Met Sleep Glial cells Drosophila mushroom body PDF projection

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Three dimensional printing of metamaterial embedded geometrical optics(MEGO)

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Microsystems & Nanoengineering 2019年第1期5卷 542-551页

作者： Aydin Sadeqi Hojatollah Rezaei Nejad Rachel E.Owyeung Sameer Sonkusale Nano lab Department of Electrical and Computer EngineeringTufts UniversityMedfordMA 02155USA Department of Chemical and Biological Engineering Tufts UniversityMedfordMA 02155USA

Three-dimensional printers have revolutionized many scientific fields with its low-cost,accessibility and ease of printing.In this paper,we show how stereolithography(SLA)based 3D printers can enable realization of innovative 3D optical devices formed through the fusion of metamaterials with geometrical optics or MEGO.It utilizes a combination of desktop SLA 3D printer and metal deposition/coating systems.Using this approach,we present innovative metamaterial embedded optical components such as mushroom-type metamaterials,curved wide-angle metamaterial absorbers/reflectors and a frequency selective moth eye hemispherical absorber.Finally a unique MEGO device formed through the fusion of a frequency selective metamaterial with an optical parabolic reflector has been demonstrated that combines their individual properties in a single device.The fabricated MEGO devices operate in the millimeter wave frequency range.Simulation and measurement results using terahertz continuous-wave spectrometer validate their functionality and performance.With improving resolution in 3D printing,MEGO devices will be able to reach Terahertz and optical frequencies in the near future.

关键词： millimeter printing optics

Scleroderma nastii sp.nov.,a gasteroid mushroom from Phulchoki hill,Nepal

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Studies in Fungi 2020年第1期5卷 50-58页

作者： Raut JK Basukala O Shrestha R Poudel RC Faculty of Science Nepal Academy of Science&Technology(NAST)KhumaltarLalitpur GPO Box 3323 KathmanduNepal Current affiliation Tumour Virology lab International Centre for Genetic Engineering and Biotechnology(ICGEB)Padriciano 9934149 TriesteItaly

A new species of Scleroderma is described from temperate Oak forest of Phulchoki hill,at the highest peak(2,762 m)ringing the Kathmandu valley,Nepal.Smooth to velvety,pseudostipitate basidiome,smaller and sub-reticulate basidiospores in comparison to its close relatives,are key diagnostic features of this newly discovered mushroom.The novelty of the taxon was also confirmed based on phylogenetic analysis using the data sets of the internal transcribed spacer region(ITS)of nuclear rDNA sequences along with the analysis of scanning electron micrographs of its basidiospores.A comprehensive description,illustrations,and comparisons with phenetically similar species are provided in this study.Additional surveys are needed to verify the species diversity and clarify their geographic distribution.

关键词： Ectomycorrhiza Lalitpur Oak forest Phylogeny Pseudostipe