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Advances in Microbiology 2019年第1期9卷 21-37页

作者： Shijie Zheng Shude Yang Xianhao Cheng Tolgor Bau Yu Li Rui Zhang Haiying Bao Engineering Research Center Chinese Ministry of Education for Edible and Medicinal Fungi Jilin Agricultural University Changchun China Shandong Key lab of Edible mushroom Technology Ludong University Yantai China

The interaction between dehydroeburicoic acid (DeEA), a triterpene purified from medicinal fungi and the major transport protein, human serum albumin (HSA), were systematically studied by fluorescence spectroscopy, synchronous fluorescence spectroscopy, three-dimensional fluorescence spectroscopy and molecular docking approach under simulated physiological conditions. The intrinsic fluorescence of HSA was quenched through the combination of static and dynamic quenching mechanism. DeEA cannot be stored and carried by HSA in the body at higher temperature. The hydrogen bonding, hydrophobic force and van der Waals force were major acting forces. The site II was the major binding site. The energy transfer could occur with high probability and the binding distance was 3.29 nm. The binding process slightly changed the conformation and microenvironment of HSA. The DeEA molecule entered the hydrophobic cleft of HSA and formed the hydrogen bonding with Glu-492 and Lys-545.

关键词： Human Serum Albumin Dehydroeburicoic Acid Interaction Fluorescence Molecular Docking

Probing the Molecular Interaction between Verbascoside and Human Serum Albumin by Fluorescence Spectroscopy and Molecular Docking

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Probing the Molecular Interaction between Verbascoside and H...

Innovative Consortia of Micro and Macro Fungal Systems: Cellulolytic Enzyme Production from Groundnut Shell Biomass and Supportive Structural Analysis

多彩菌物美丽中国——中国菌物学会2019年学术年会

作者： Shijie Zheng Shude Yang Rui Zhang Xianhao Cheng Haiying Bao Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi Jilin Agricultural University ShanDong Key lab of Edible mushroom Technology Ludong University

The mechanism and conformational changes of the interaction between verbascoside（VB） and human serum albumin（HSA） were systematically studied by fluorescence spectroscopy, synchronous fluorescence spectroscopy, three-dimensional fluorescence spectroscopy, and molecular docking. The intrinsic fluorescence of HSA was quenched by VB through the combination of static and dynamic quenching mechanism. According to the Scatchard equation, the binding constant and the number of binding site were evaluated. The thermodynamic parameters showed that the van der Waals forces and the hydrogen bonding were dominant in the binding *** displacement experiments with site markers indicated that VB bound to HSA at site II（subdomain IIIA）. The synchronous fluorescence spectroscopy and three-dimensional fluorescence spectroscopy results suggested that the interaction between VB and HSA changed the polarity and microenvironment around tyrosine residues. The interaction between VB and the amino acid residues of HSA was shown by molecular docking approach, and the theoretical results were consistent with experimental results.

关键词： verbascoside interaction human serum albumin fluorescence molecular docking

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Journal of Sustainable Bioenergy Systems 2018年第3期8卷 47-66页

作者： Sai Praneeth Thota Pradeep Kumar Badiya Yadhu Nadh Guragain Praveen Venkata Vadlani Meera Pandey Rajesh Babu Dandamudi Sai Sathish Ramamurthy Siva Kumar Belliraj Department of Chemistry Sri Sathya Sai Institute of Higher Learning Prasanthi Nilayam India Bioprocessing and Renewable Energy laboratory Departments of Grain Science and Industry & Chemical Engineering Kansas State University Manhattan KS USA mushroom lab Indian Institute Horticulture Research Bangalore India

Renewable biomass-derived fuels are essential to meet the blend mandates and the sustainability goals. In our first-to-date study, we investigated individual and synergistic consortia of fungal cultures comprising Pycnoporous sanguineus (PS) in combination with Aspergillus oryzae (AO) and Trichoderma harzianum (TH) for production of cellulolytic enzymes using groundnut shell under solid state and submerged liquid fermentation conditions. The innovative consortia closely align with the microbial ecosystems found in nature;consequently, we anticipate a potent and effective cellulolytic enzyme system, which maximises the breakdown of biomass polymers to sugars. Under ternary combination of cultures, cellulase production was highest at 123.0 ± 1 FPU/gds;β-glucosidase production at 875.6 ± 26.4 IU/g dry substrate (gds);and CMCase at 474.95 ± 45.5 IU/gds. β-glucosidase production was highest on the 2nd day at 987.03 ± 64.2 IU/gds while CMCase peaked at 514.97 ± 21.4 IU/gds on 2nd day for PS, maximum cellulase enzyme production was observed on the 6th day at 192.2 ± 0.96 FPU/gds (AO + PS). Present work showed that synergistic combination of fungal cultures for releasing balanced enzyme activities that can efficiently saccharify biomass, such as groundnut shell to sugars, which can subsequently be fermented to various bioproducts and biomaterial monomers. Elaborate characterization studies of enzyme treated groundnut shell revealed prominent physicochemical changes in the hydrolysate, which indicates the changes in biomass are due to the enzymatic action and the growth effects of the consortia;thereby, leading to promising applications for the microbial fortified biomass.

关键词： Mixed Cultures Fermentation Enzymatic Hydrolysis Groundnut Shell Biomass Characterization

Enhanced Compliant Adhesive Design and Fabrication with Dual-Level Hierarchical Structure

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Journal of Bionic Engineering 2010年第3期7卷 228-234页

作者： Dan Sameoto Carlo Menon Department of Mechanical Engineering University of Alberta MENRVA lab School of Engineering ScienceSimon Fraser University

Synthetic dry adhesives inspired by the nano-and micro-scale hairs found on the feet of geckos and some spiders have beendeveloped for almost a decade. Elastomeric single level micro-scale mushroom shaped fibres are currently able to function evenbetter than natural dry adhesives on smooth surfaces under normal loading. However, the adhesion of these single level syntheticdry adhesives on rough surfaces is still not optimal because of the reduced contact surface area. In nature, contact area ismaximized by hierarchically structuring different scales of fibres capable of conforming surface roughness. In this paper, weadapt the nature’s solution arid propose a novel dual-level hierarchical adhesive design using Polydimethylsiloxane (PDMS),which is tested under peel loading at different orientations. A negative macro-scale mold is manufactured by using a laser cutterto define holes in a Poly(methyl methacrylate) (PMMA) plate. After casting PDMS macro-scale fibres by using the obtainedPMMA mold, a previously prepared micro-fibre adhesive is bonded to the macro-scale fibre substrate. Once the bondingpolymer is cured, the micro-fibre adhesive is cut to form macro scale mushroom caps. Each macro-fibre of the resulting hierarchicaladhesive is able to conform to loads applied in different directions. The dual-level structure enhances the peel strengthon smooth surfaces compared to a single-level dry adhesive, but also weakens the shear strength of the adhesive for a given areain contact. The adhesive appears to be very performance sensitive to the specific size of the fibre tips, and experiments indicatethat designing hierarchical structures is not as simple as placing multiple scales of fibres on top of one another, but can requiresignificant design optimization to enhance the contact mechanics and adhesion strength.

关键词： biomimetics dry adhesive hierarchical dual-level adhesive silicone polymer

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A Simplified Computational Model of mushroom Body for Tethered Bees' Abdominal Swing Behavior Induced by Optic Flow

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Chinese Journal of Electronics 2021年第2期30卷 296-302页

作者： ZHENG Nenggan MA Qian WANG Xuefei ZHAO Lei GONG Zhefeng Qiushi Academy for Advanced Studies Zhejiang University College of Computer Science and Technology Zhejiang University Department of Neurobiology Zhejiang University School of Medicine Zhejiang lab

Understanding sensorimotor neural circuits plays an important role in the study of behavioral mechanisms. By virtue of a relatively simple brain structure and sophisticated locomotion behaviors, insects are selected as comparative research subjects to discover the basic principles of neural science. Specific abdominal swing behaviors of tethered bees induced by optomotor response are realized. To model functionality of mushroom body in the optic-flow induced swing behaviors, a simplified 3-layer Spiking neural network(SNN) is proposed. Spike response model is used as the single neuron model in the proposed SNN, which is trained by supervised learning method. The computational model can accurately simulate and predict the bees’ abdominal swing behaviors exhibiting ipsilateral direction and proportional frequencies with optic flow stimulus.

关键词： image sequences optic flow stimulus tethered bees induced neurophysiology SNN neural science brain specific abdominal swing biomechanics mobile robots comparative research subjects neurocontrollers single neuron model simplified computational model neural network neural nets learning (artificial intelligence) behavioral mechanisms mushroom body optomotor response sophisticated locomotion behaviors optic-flow induced swing behaviors spike response model sensorimotor neural circuits relatively simple brain structure

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淡水真菌三个中国新记录种

菌物学报 2011年第1期30卷 123-127页

作者：陈燕李秀岚蔡磊金静张剑锋 Hyde D Kevin 路炳声青岛农业大学农学与植物保护学院青岛266109 青岛农业大学化学与药学院青岛266109 中国科学院微生物研究所北京100110 mushroom Research Foundation Thailand

在进行我国淡水真菌多样性的研究中,发现3个中国新记录种:墨鱼状假黑囊壳Pseudomassaria sepincoliformis,二隔四壳座菌Quaternaria dissepta和萨尼亚小镰孢Fusariella sarniensis。文中对每个种进行了形态描述及讨论,标本保存于青岛农... 详细信息

在进行我国淡水真菌多样性的研究中,发现3个中国新记录种:墨鱼状假黑囊壳Pseudomassaria sepincoliformis,二隔四壳座菌Quaternaria dissepta和萨尼亚小镰孢Fusariella sarniensis。文中对每个种进行了形态描述及讨论,标本保存于青岛农业大学菌物标本室。

关键词：中国新记录种真菌多样性子囊菌海南甘肃

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Three dimensional printing of metamaterial embedded geometrical optics(MEGO)

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Microsystems & Nanoengineering 2019年第1期5卷 542-551页

作者： Aydin Sadeqi Hojatollah Rezaei Nejad Rachel E.Owyeung Sameer Sonkusale Nano lab Department of Electrical and Computer EngineeringTufts UniversityMedfordMA 02155USA Department of Chemical and Biological Engineering Tufts UniversityMedfordMA 02155USA

Three-dimensional printers have revolutionized many scientific fields with its low-cost,accessibility and ease of *** this paper,we show how stereolithography(SLA)based 3D printers can enable realization of innovative 3D optical devices formed through the fusion of metamaterials with geometrical optics or *** utilizes a combination of desktop SLA 3D printer and metal deposition/coating *** this approach,we present innovative metamaterial embedded optical components such as mushroom-type metamaterials,curved wide-angle metamaterial absorbers/reflectors and a frequency selective moth eye hemispherical *** a unique MEGO device formed through the fusion of a frequency selective metamaterial with an optical parabolic reflector has been demonstrated that combines their individual properties in a single *** fabricated MEGO devices operate in the millimeter wave frequency *** and measurement results using terahertz continuous-wave spectrometer validate their functionality and *** improving resolution in 3D printing,MEGO devices will be able to reach Terahertz and optical frequencies in the near future.

关键词： millimeter printing optics

Juvenile hormone receptor Met regulates sleep and neuronal morphology via glial-neuronal crosstalk

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Journal of Genetics and Genomics 2021年第8期48卷 706-715页

作者： Binbin Wu Lei He Yutong Xiao Juan Du Xiaoxiao Wang Zhangwu Zhao Department of Entomology and MOA Key lab of Pest Monitoring and Green Management College of Plant ProtectionChina Agricultural UniversityBeijing 100193China College of Life Sciences Hebei UniversityBaoding 071002China

Juvenile hormone(JH)is one of the most important hormones in insects since it is essential for insect *** mechanism by which JH affects the central nervous system still remains a *** this study,we demonstrate that one of the JH receptors,Methoprene-tolerant(Met),is important for the control of neurite development and sleep behavior in *** the identification of Met-expressing glial cells,the mechanism that Met negatively controls the mushroom body(MB)b lobes fusion and positively maintains pigment-dispersing factor s LNvs projection pruning has been ***,despite the developmental effects,Met can also maintain nighttime sleep in a developmentindependent manner through the a/b lobe of *** analyses of neuronal morphology and entomological behavior,this study advances our understanding of how the JH receptor regulates the nervous system.

关键词： Met Sleep Glial cells Drosophila mushroom body PDF projection

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Scleroderma nastii ***.,a gasteroid mushroom from Phulchoki hill,Nepal

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Studies in Fungi 2020年第1期5卷 50-58页

作者： Raut JK Basukala O Shrestha R Poudel RC Faculty of Science Nepal Academy of Science&Technology(NAST)KhumaltarLalitpur GPO Box 3323 KathmanduNepal Current affiliation Tumour Virology lab International Centre for Genetic Engineering and Biotechnology(ICGEB)Padriciano 9934149 TriesteItaly

A new species of Scleroderma is described from temperate Oak forest of Phulchoki hill,at the highest peak(2,762 m)ringing the Kathmandu valley,*** to velvety,pseudostipitate basidiome,smaller and sub-reticulate basidiospores in comparison to its close relatives,are key diagnostic features of this newly discovered *** novelty of the taxon was also confirmed based on phylogenetic analysis using the data sets of the internal transcribed spacer region(ITS)of nuclear rDNA sequences along with the analysis of scanning electron micrographs of its basidiospores.A comprehensive description,illustrations,and comparisons with phenetically similar species are provided in this *** surveys are needed to verify the species diversity and clarify their geographic distribution.

关键词： Ectomycorrhiza Lalitpur Oak forest Phylogeny Pseudostipe

Advanced Electro-active Dry Adhesive Actuated by an Artificial Muscle Constructed from an Ionic Polymer Metal Composite Reinforced with Nitrogen-doped Carbon Nanocages

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Journal of Bionic Engineering 2017年第3期14卷 567-578页

作者： Qingsong He Xu Yang Zhongyuan Wang Jin Zhao Min Yu Zhen Hu Zhendong Dai Institute of Bio-inspired Structure and Surface Engineering Jiangsu Provincial Key Laboratory of Bionic Functional Materials Col- lege of Astronautics Nanjing University of Aeronautics & Astronautics Nanjing 210016 China College of Mechanical and Electrical Engineering Nanjing University of Aeronautics & Astronautics Nanjing 210016 China Key laboratory of Mesoscopic Chemistry of MOE Jiangsu Provincial Lab for Nanotechnology School of Chemistry and Chemical Engineering Nanjing University Nanjing 210093 China

An advanced electro-active dry adhesive, which was composed of a mushroom-shaped tibrillar dry adhesive array actuated by an Ionic Polymer Metal Composite （IPMC） artificial muscle reinforced with nitrogen-doped carbon nanocages （NCNCs）, was developed to imitate the actuation of a gecko＇s toe. The properties of the NCNC-reinforced Nation membrane, the electro- mechanical properties of the NCNC-reinforced IPMC, and the related electro-active adhesion ability were investigated. The NCNCs were uniformly dispersed in the 0.1 wt% NCNC/Nafion membrane, and there was a seamless connection with no clear interface between the dry adhesive and the IPMC. Our 0.1 wt% NCNC/Nation-IPMC actuator shows a displacement and force that are 1.6 - 2 times higher than those of the recast Nafion-IPMC. This is due to the increased water uptake （25.39%） and tensile strength （24.5 MPa） of the specific 3D hollow NCNC-reinforced Nation membrane, as well as interactions between the NCNCs and the sulfonated groups of the Nation. The NCNC/Nation-IPMC was used to effectively actuate the mushroom-shaped dry adhesive. The normal adhesion forces were 7.85 raN, 12.1 mN, and 51.7 mN at sinusoidal voltages of 1.5 V, 2.5 V, and 3.5 V, respectively, at 0.1 Hz. Under the bionic leg trail, the normal and shear forces were approximately 713.5 mN （159 mN·cm^-2） and 1256.6 mN （279 mN·cm^-2）, respectively, which satisfy the required adhesion. This new electro-active dry adhesive can be applied for active, distributed actuation and flexible grip in robots.

关键词： electro-active dry adhesive bionic ionic polymer metal composite Nation membrane carbon nanocage