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Expression of growth hormone receptor and its mRNA in hepatic cirrhosis

World Journal of Gastroenterology 2003年第4期9卷 765-770页

作者： Hong-TaoWang ShuangChen JieWang Qing-JiaOu ChaoLiu Shu-SenZheng Mei-HaiDeng Xiao-PingLiu DepartmentofHepato-biliarySurgery SunYat-SenMemorialHospitaltheSecondAffiliatedHospitalSunYat-SenUniversityGuangzhou510120GuangdongProvinceChina DepartmentofGastroenteralSurgery SunYat-SenMemorialHospitaltheSecondAffiliatedHospitalSunYat-SenUniversityGuangzhou510120GuangdongProvinceChina molecularmedicallaboratorycenter SunYat-SenMedicalCollegeSunYat-SenUniversityGuangzhou510089GuangdongProvinceChina DepartmentofGeneralSurgery theThirdAffiliatedHospitalSunYat-SenUniversityGuangzhou510630GuangdongProvinceChina DepartmentofGeneralSurgery ShenzhenCentralHospitalPekingUniversityShenzhen518036China

AIM: To investigate the expression of growth hormone receptor (GHR) and mRNA of GHR in cirrhotic livers of rats with the intension to find the basis for application of recombinant human growth hormone (rhGH) to patients with liver ***: Hepatic cirrhosis was induced in SpragueDawley rats by administration of thioacetamide intraperitoneally for 9-12 weeks. Collagenase Ⅳ was perfused in situ for isolation of hepatocytes. The expression of GHR and its mRNA in cirrhotic livers was studied with radio-ligand binding assay, RT-PCR and digital image ***: One class of specific growth hormone-binding site, GHR, was detected in hepatocytes and hepatic tissue of cirrhotic livers. The binding capacity of GHR (RT, fmol/mg protein) in rat cirrhotic liver tissue (30.8±1.9) was significantly lower than that in normal control (74.9±3.9) at the time point of the ninth week after initiation of induction of cirrhosis (n=10, P＜0.05), and it decreased gradually along with the accumulation of collagen in the process of formation and development of liver cirrhosis (P＜0.05). The number of binding sites (×10 4/cell) of GHR on rat cirrhotic hepatocytes (0.86±0.16) was significantly lower than that (1.28±0.24)in control (n= 10, P＜0.05). The binding affinity of GHR among liver tissue, hepatocytes of various groups had no significant difference (P＞0.05). The expression of GHR mRNA (riOD,pixel) in rat cirrhotic hepatic tissues (23.3±3.1) was also significantly lower than that (29.3±3.4) in normal control (n=10, P＜0.05).CONCLUSION: The growth hormone receptor was expressed in a reduced level in liver tissue of cirrhotic rats,and lesser expression of growth hormone receptors was found in a later stage of cirrhosis. The reduced expression of growth hormone receptor was partly due to its decreased expression on cirrhotic hepatocytes and the reduced expression of its mRNA in cirrhotic liver tissue.

关键词：生长激素受体肝硬化 mRNA 逆转录聚合酶链反应慢性肝病免疫反应

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