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Significance of a novel sucrose permeability test using serum in the diagnosis of early gastric cancer

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World Journal of Gastroenterology 2005年第44期11卷 6905-6909页

作者： Tadayuki Shishido Taketo Yamaguchi Takeo Odaka Masanori Seimiya Hiromitsu Saisho Fumio Nomura Department of Medicine and Clinical oncology Department of molecular Diagnosis

AIM： To investigate the usefulness of sucrose permeability test using serum in the diagnosis of gastric diseases, with special reference to early gastric cancer （EGC）. METHODS： A total of 63 subjects, including 11 patients with gastric ulcer, 20 patients with gastric cancer （13, early; 7, advanced） and 32 healthy controls, were studied. Blood and urine samples were collected repeatedly for 5 h before and after the sucrose loading. Sucrose levels were measured by a newly developed RESULTS： Serum sucrose levels started to increase 15 min after loading, and peaked at 60 min in the gastric disease groups. The levels for gastric ulcer, EGC and advanced gastric cancer （AGC） at 60 min were significantly higher than that in the healthy controls （26.9±2.4, 34.4±5.0, and 71.8±15.6 vs 7.9±0.7 mol/L, respectively, P〈0.01）. The cut-off level set at 15.4 mol/L （60 min） offered the best distinction between EGC patients and healthy controls; and the sensitivity and specificity were 92.3% and 93.8%, respectively, while those of the urine method were 76.9% and 93.8%, respectively. CONCLUSIONS： The gastric permeability test using serum is reliable for the detection of EGC, and this test can provide results much earlier than the conventional urine method. This test may offer a useful alternative to more invasive tests for EGC.

关键词： Sucrose test Gastric permeability Early gastric cancer Advanced gastric cancer Gastric ulcer

Construction and Expression of a Single Chain Antibody Mimicing Human Ovarian Cancer Antigen CA125

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Cellular & molecular Immunology 2006年第1期3卷 59-62页

作者： Aidong Li Zheng Li Yinghong Wang Yongming Zhang Jie Ma Central laboratory Cancer Institute and Hospital Chinese Academy of Medical Sciences PUMC Panjiayuan Beijing 100021 China State Key laboratory of molecular oncology Cancer Institute and Hospital Chinese Academy of Medical Sciences China State Key laboratory of molecular oncology Cancer Institute Chinese Academy of Medical Sciences PUNIC Panjiayuan Beijing 100021 China

One concept for immune therapy of cancer involves induction of antigen mimic antibodies to trigger the immune response against tumor cells. Anti-idiotypic antibodies directed against the antigen-binding site of antibodies specific for tumor antigen may functionally and even structurally mimic antigen and induce anti-anti-idiotypic immune response. Monoclonal antibody W J02 is one of such anti-idiotypic antibodies, which contains internal image of CA125. In order to improve the immunospecificity of mAb WJ02, we constructed a single chain of mAb WJ02 in Vl-linker-Vh orientation. The scFv-WJ02, could be expressed and secreted in the recombinant Pichia pastoris system. The secreted scFv protein with a molecular weight of 30 kD retained the biological activity of mAb WJ02, which was proved by a direct binding assay and inhibition experiment. Our results indicated that the scFv-WJ02 could be used as a possible tool for idiotypic therapy against ovarian cancer, which might enhance the possibility of eliminating nonspecific responses induced by mAb WJ02.

关键词： anti-idiotype single chain antibody P. pastoris

Cysteine-113 covalency inspires the development of Pin1 inhibitor

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Signal Transduction and Targeted Therapy 2020年第1期5卷 643-645页

作者： Wenchen Pu Jiao Li Yong Peng laboratory of molecular oncology Frontiers Science Center for Disease-related Molecular NetworkState Key Laboratory of Biotherapy and Cancer CenterWest China HospitalSichuan UniversityChengdu 610064China

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1(Pin1)controls the functional switch of phosphoproteins and plays an oncogenic role in human cancer,but the discovery of effective Pin1 inhibitors remains challenging.A recent study by Pinch et *** a novel Pin1 inhibitor by endowing molecule with covalent reactivity toward Pin1 cysteine-113,1 providing new insights into the design of anticancer Pin1 inhibitors.

关键词： Pin1 al insight

Super-resolution imaging and tracking of TGF-β receptor II on living cells

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Science Bulletin 2016年第8期61卷 632-638页

作者： Zi Ye Nan Li Libo Zhao Yahong Sun Hefei Ruan Mingliang Zhang Jinghe Yuan Xiaohong Fang Key laboratory of molecular Nanostructure and Nanotechnology Beijing National Laboratory for Molecular Sciences Institute of Chemistry Chinese Academy of Sciences

Single-particle tracking photoactivated local- ization microscopy （sptPALM） has recently emerged as a powerful tool for high-density imaging and tracking of individual molecules in living cells. In this work, we have monitored and compared the diffusion dynamics of TGF-β type II receptor （TβRII） at high expression level using both traditional single-particle tracking （SPT） and sptPALM. The ligand-induced aggregation of TβRII oligomers was further indicated by sptPALM. Due to the capacity of distinguishing and tracking single molecules within diffraction limit, sptPALM outperforms traditional SPT by providing more accurate biophysical information,

关键词： Single-particle tracking Photoactivatedlocalization microscopy - Single-moleculefluorescence imaging TGF-β receptor II Membranediffusion

Analysis of gene expression profile induced by EMP-1 in esophageal cancer cells using cDNA Microarray

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World Journal of Gastroenterology 2003年第3期9卷 392-398页

作者： Hai-Tao Wang Jian-Ping Kong Fang Ding Xiu-Qin Wang Ming-Rong Wang Lian-Xin Liu Min Wu Zhi-Hua Liu National laboratory of molecular Ontology, Cancer Institute, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100021, China National laboratory of molecular oncology Cancer Institute Chinese Academy of Medical Science and Peking Union Medical College Beijing 100021 China.

AIM: To obtain human esophageal cancer cell EC9706 stably expressed epithelial membrane protein-1 (EMP-1) with integrated eukaryotic plasmid harboring the open reading frame (ORF) of human EMP-1, and then to study the mechanism by which EMP-1 exerts its diverse cellular action on cell proliferation and altered gene profile by exploring the effect of ***: The authors first constructed pcDNA3.1/mychis expression vector harboring the ORF of EMP-1 and then transfected it into human esophageal carcinoma cell line EC9706. The positive clones were analyzed by Western blot and RT-PCR. Moreover, the cell growth curve was observed and the cell cycle was checked by FACS technique. Using cDNA microarray technology, the authors compared the gene expression pattern in positive clones with control. To confirm the gene expression profile, semi-quantitative RT-PCR was carried out for 4 of the randomly picked differentially expressed genes. For those differentially expressed genes,classification was performed according to their function and cellular ***: Human EMP-1 gene can be stably expressed in ECg706 cell line transfected with human EMP-1. The authors found the cell growth decreased, among which S phase was arrested and G1 phase was prolonged in the transfected positive clones. By cDNA microarray analysis, 35 genes showed an over 2.0 fold change in expression level after transfection, with 28 genes being consistently up-regulated and 7 genes being down-regulated. Among the classified genes, almost half of the induced genes (13 out of 28 genes) were related to cell signaling, cell communication and particularly to ***: Overexpression of human EMP-1 gene can inhibit the proliferation of EC9706 cell with S phase arrested and G1 phase prolonged. The cDNA microarray analysis suggested that EMP-1 may be one of regulators involved incell signaling, cell communication and adhesion regulators.

关键词：上皮膜蛋白-1 食管癌 Western blot法 RT-PCR 基因表达 cDNA微阵列

Loss of Heterozygosity in Esophageal Carcinoma

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Chinese Science Bulletin 1993年第5期38卷 413-418页

作者：肖广惠李万波黄建华吴旻 National laboratory of molecular oncology Department of Cell Biology Cancer Institute CAMS Beijing 100021 PRC

Esophageal cancer (EC) is one of the most common malignancies in China. Genetic epidemiologic surveys carried out in high-incidence areas of North China have revealed that some genetic factors are involved in pathogenesis of this human cancer. Cytogenetic analyses have shown that nonrandom chromosomal rearrangements, including breakage, translocation and deletion, can be found in esophageal cancer cells as well as in

关键词： esophageal cancer loss of heterozygosity RFLP.

Stable oncogenic transformation induced by microcell-mediated gene transfer

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Science China Chemistry 1995年第4期38卷 448-456页

作者：吕有勇 Donald G.Blair Beijing Institute for Cancer Research Beijing 100034 China laboratory of molecular oncology National Cancer Institute Frederick MD 21702-1013 USA

Oncogenes have been identified using DNA-mediated transfection, but the size of the transferable and unrearranged DNA, gene rearrangement and amplification which occur during the transfection process limit the use of the techniques. We have evaluated microcell-mediated gene transfer techniques for the transfer and analysis of dominant oncogenes. MNNG-HOS, a transformed human cell line which contained the met oncogene mapping to human chromosome 7 was infected with retroviruses carrying drug resistance markers and used to optimize microcell preparation and transfer. Stable and drug-resistant hybrids containing single human chromosomes as well as the foci of the transformed cells containing the activated met oncogene and intact hitman chromosomes were obtained. Hybridization analysis with probes (i.e. collA2, pJ3.11) mapping up to 1 Mb away from met shows that the cells from the individual focr contain different amounts of apparently unrearranged human DNA associated with the oncogene, and the microcell-generated transformants retain more distal markers than those observed in either DNA- or chromosome-mediated transfers. In conjunction with other techniques, microcell fusion should be useful for gene mapping as well as the study of gene function and expression in cell transformation and malignancy.

关键词： oncogene microcell cell transformation gene mapping met.

Genetic and epigenetic dependencies in colorectal cancer development

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Gastroenterology Report 2022年第1期10卷 115-131页

作者： Sehej Parmar Hariharan Easwaran Cancer Genetics and epigenetics OncologySidney Kimmel Comprehensive Cancer CenterJohns Hopkins University School of MedicineBaltimoreMDUSA

Recent studies have mapped key genetic changes in colorectal cancer(CRC)that impact important pathways contributing to the multistep models for CRC initiation and *** parallel with genetic changes,normal and cancer tissues harbor epigenetic alterations impacting regulation of critical genes that have been shown to play profound roles in the tumor ***,these molecular changes are only loosely associated with heterogenous transcriptional programs,reflecting the heterogeneity in the various CRC molecular subtypes and the paths to CRC *** from mapping molecular alterations in early CRC lesions and use of experimental models suggest that the intricate dependencies of various genetic and epigenetic hits shape the early development of CRC via different pathways and its manifestation into various CRC *** highlight the dependency of epigenetic and genetic changes in driving CRC development and discuss factors affecting epigenetic alterations over time and,by extension,risk for cancer.

关键词： colorectal cancer epigenetics aging classification subtyping DNA methylation consensus molecular subtypes

TRANSTHYRETIN (PREALBUMIN) GENE IN HUMAN PRIMARY HEPATIC CANCER

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Science China Chemistry 1991年第11期34卷 1312-1318页

作者：顾建人蒋惠秋贺丽萍李岱宗周筱梅戴卫列钱莲芳陈渊卿 C. SCHWEINFEST T. PAPAS Notional laboratory for Oncogenes and Related Genes Shanghai Cancer InstituteShanghai 200032 PRC laboratory of molecular oncology National Cancer Institute(NCI-FCRDC)USA

From a subtracting cDNA library constructed from normal liver versus human primary hepatic cancer (PHC), a cDNA clone pG8 was isolated. Using it as a probe, RNA extracted from one human liver and 9 PHC samples were analyzed by Northern hybridization. As expected, its mRNA was highly expressed in liver; however, the expression was strikingly suppressed in PHC. Only weak signal was observed in 2 out of 9 PHC, while no signal was detectable in the other 7 samples. Utilizing pG8 as a probe, DNA from the same PHC specimens was analyzed after MspI digestion and Southern hybridization. Deletion of DNA fragment was observed in 4 out of 9 samples. In further study of cancer and non-cancerous liver from other 7 PHC patients, similar deletion of DNA fragments in cancer was observed in 4 out of 7 samples. After sequencing of the clone of 572 bp, it was unexpectedly found that pG8 was completely homologous to the coding sequence of transthyretin, TTR gene, as TTR (or prealbumin) gene has been known to be linked to a hereditary disorder, familial amyloidosis (FAP), and related to thyroxine transport and binding to retinol-RBP (the retinol binding protein) complex. This is the first report of a study on TTR in human primary hepatic cancer. Since TTR gene was strikingly suppressed in mRNA expression and possibly defective in its gene structure, it was strongly implicated that TTR might be an important gene marker or a candidate of anti-oncogene for human PHC. The biological activity of TTR gene is under study.

关键词： hepatoma subtracting cDNA library suppression deletion.