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Hematopoietic aging:Cellular,molecular,and related mechanisms

Chinese Medical Journal 2024年第11期137卷 1303-1312页

作者： Li Ye Chuan Tian Ye Li Hang Pan Jinxiu Hu Liping Shu Xinghua Pan The Stem Cells and Immune Cells Biomedical Techniques Integrated Engineering Laboratory of State and Regions Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan ProvinceKunming Key Laboratory of Stem Cell and Regenerative MedicineBasic Medical Laboratory920th Hospital of Joint Logistics Support ForcePLAKunmingYunnan 650032China Department of Immunology of School of Basic Medicine of Guizhou Medical University National and Local Joint Engineering Laboratory of Cell Engineering Biomedical TechnologyKey Laboratory of Regenerative Medicine of Guizhou ProvinceState Key Laboratory of Efficacy and Utilization of Medicinal Plants Co-constructed by Province and MinistryKey Laboratory of Translational Research of Adult Stem Cell of Chinese Academy of Medical SciencesGuiyangGuizhou 550025China

Aging is accompanied by significant inhibition of hematopoietic and immune system function and disruption of bone marrow ***-related alterations in the inflammatory response,immunity,and stem cell niches are at the root of hematopoietic *** the molecular mechanisms underlying hematopoietic and bone marrow aging can aid the clinical treatment of aging-related *** particular,it is unknown how the niche reprograms hematopoietic stem cells(HSCs)in an age-dependent manner to maintain normal hematopoiesis in elderly ***,specific inhibitors and blood exchange methods have been shown to reshape the hematopoietic niche and reverse hematopoietic ***,we present the latest scientific discoveries related to hematopoietic aging and hematopoietic system rejuvenation,discuss the relationships between hematopoietic niche aging and HSC aging,and describe related studies on stem cell-mediated regulation of hematopoietic aging,aiming to provide new ideas for further study.

关键词： Stem cells Hematopoietic stem cell Niche Hematopoietic aging

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Heteromeric and Homomeric Geranyl Diphosphate Synthases from Catharanthus roseus and Their Role in Monoterpene Indole Alkaloid Biosynthesis

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molecular Plant 2013年第5期6卷 1531-1549页

作者： Avanish Rai Shachi S. Smita Anup Kumar Singh Karuna Shanker Dinesh A. Nagegowda CSIR-Central Institute of Medicinal and Aromatic plants Research Centre Bangalore 560065 India CSIR-Central Institute of Medicinal and Aromatic plants Lucknow 226015 India

Catharanthus roseus is the sole source of two most important monoterpene indole alkaloid （MIA） anti- cancer agents： vinblastine and vincristine. MIAs possess a terpene and an indole moiety derived from terpenoid and shikimate pathways, respectively. Geranyl diphosphate （GPP）, the entry point to the formation of terpene moiety, is a product of the condensation of isopentenyl diphosphate （IPP） and dimethylallyl diphosphate （DMAPP） by GPP synthase （GPPS）. Here, we report three genes encoding proteins with sequence similarity to large subunit （***） and small subunit （***） of heteromeric GPPSs, and a homomeric GPPSs. *** is a bifunctional enzyme producing both GPP and geranyl geranyl diphosphate （GGPP）, *** is inactive, whereas CrGPPS is a homomeric enzyme forming GPP. Co-expression of both subunits in Escherichia coil resulted in heteromeric enzyme with enhanced activity producing only GPR While *** and CrGPPS showed higher expression in older and younger leaves, respectively, *** showed an increasing trend and decreased gradually. Methyl jasmonate （MelA） treatment of leaves sig- nificantly induced the expression of only ***. GFP localization indicated that *** is plastidial whereas CrGPPS is mitochondrial. Transient overexpression of *** in C. roseus leaves resulted in increased vindoline, immediate monomeric precursor of vinblastine and vincristine. Although C. roseus has both heteromeric and homomeric GPPS enzymes, our results implicate the involvement of only heteromeric GPPS with *** regulating the GPP flux for MIA biosynthesis.

关键词： C. roseus monoterpene indole alkaloids geranyl diphosphate synthase heteromer homomer large subunit small subunit vindoline.

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The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in plants: A Comprehensive Review

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Phyton-International Journal of Experimental Botany 2024年第6期93卷 1325-1344页

作者： Muhammad Saleem Jianhua Zhang Muhammad Qasim Rashid Iqbal Li Song Joint International Research Laboratory of Agriculture and Agri-Product Safety The Ministry of Education of ChinaJiangsu Key Laboratory of Crop Genomics and Molecular BreedingYangzhou UniversityYangzhou225009China Microelement Research Center College of Resources and EnvironmentHuazhong Agricultural UniversityWuhan430070China Department of Agronomy Faculty of Agriculture and EnvironmentThe Islamia University of BahawalpurBahawalpur63100Pakistan

Variations in the nutrients and water that plants require for metabolism,development,and the maintenance of cellular homeostasis are the main causes of abiotic stress in *** has,however,hardly ever been studied how these transporter proteins,such as aquaporin which is responsible for food and water intake in cell plasma mem-branes,interact with one *** review aims to explore the interactions between nutrient transporters and aquaporins during water and nutrient *** also investigates how symbiotic relationships inﬂuence the plant genome’s responses to regulatory processes such as photoperiodism,senescence,and nitrogenﬁ*** responses are observed in reaction to various abiotic *** instance,plasma membrane transporters are upregulated during macronutrient insufﬁciency,tonoplast transporters are overexpressed,and aquaporins are downregulated in micronutrient deﬁ***,tolerant plants often exhibit increased expression of nutrient transporters and aquaporins in response to drought,salt,and cold *** better comprehend plant stress tolerance to abiotic challenges including starvation,K famine,salt,and freezing temperatures,both classes of nutrient and water transporters should be considered at the same time.

关键词： Nutrients abiotic stress aquaporin transporters

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Volatile metabolome and transcriptome reveal fragrance release rhythm and molecular mechanisms of Rosa yangii

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Journal of Integrative Agriculture 2023年第7期22卷 2111-2125页

作者： ZHOU Li-jun HUANG Run-huan LIU Ting-han LIU Wei-chao CHEN Yun-yi LÜPei-feng LUO Le PAN Hui-tang YU Chao ZHANG Qi-xiang Beijing Key Laboratory of Ornamental plants Germplasm Innovation&molecular Breeding/National Engineering Research Center for Floriculture/Beijing Laboratory of Urban and Rural Ecological Environment/Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental plants of Ministry of Education/School of Landscape Architecture Beijing Forestry UniversityBeijing 100083P.R.China

Rose is a highly significant ornamental plant with substantial edible and medicinal value,cultivated worldwide primarily for perfume ***,Rosa yangii,a new species found in northwestern Yunnan,China,has drawn attention due to its strong sweet scented *** this study,the floral components of *** were extracted at different flowering stages using solid phase micro extraction(SPME)and analyzed through gas chromatography-mass spectrometry(GC-MS).A total of 131 volatile organic compounds(VOCs)were detected from ***,including 69 odor *** production and release of floral VOCs were the highest during the initial-open stage,making it the most suitable time for harvesting as a significant number of floral components were synthesized and *** analysis of the odor activity values(OAV)highlighted several key aromatic ingredients of ***,such as eugenol,methyleugenol,benzeneacetaldehyde and phenylethylalcohol,heptanal,decanal,(E)-2-hexen-1-yl acetate,caryophyllene,and *** and time-order gene co-expression networks(TO-GCN)revealed that VOCs and benzenoids/phenylpropanoids,along with associated genes,played a pivotal role in the overall floral regulatory network of *** and bHLH were identified as the essential regulatory factors governing the regulation of eugenol synthase(EGS)and isoeugenol synthase(IGS),consequently influencing the sweet scent of *** findings of this study provide a scientific foundation for enhancing fragrance through molecular breeding of ornamental ***,the study facilitated the development and utilization of this new plant’s essential oil material in various industries,including food storage,aromatherapy,cosmetic,and perfumery.

关键词： rose sweet fragrance SPME-GC-MS aroma VOCs TO-GCN

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Redox-Dependent Regulation of the Stress-Induced Zinc-Finger Protein SAP12 in Arabidopsis thaliana

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molecular Plant 2009年第2期2卷 357-367页

作者： Elke Stroeher Xin-Jia Wang Nils Roloff Peter Klein Arne Husemann Karl-Josef Dietz Biochemistry and Physiology of plants Bielefeld University 33501 Bielefeld Germany

The stress-associated protein SAP12 belongs to the stress-associated protein （SAP） family with 14 members in Arabidopsis thaliana. SAP12 contains two AN1 zinc fingers and was identified in diagonal 2D redox SDS-PAGE as a protein undergoing major redox-dependent conformational changes. Its transcript was strongly induced under cold and salt stress in a time-dependent manner similar to SAP10, with high levels after 6 h and decreasing levels after 24 and 48 h. The tran- script regulation resembled those of the stress marker peroxiredoxin PrxllD at 24 and 48 h. Recombinant SAP12 protein showed redox-dependent changes in quaternary structure as visualized by altered electrophoretic mobility in non-reducing SDS polyacrylamide gel electrophoresis. The oxidized oligomer was reduced by high dithiothreitol concentrations, and also by E. coli thioredoxin TrxA with low dithiothreitol （DTF） concentrations or NADPH plus NADPH-dependent thioredoxin reductase. From Western blots, the SAP12 protein amount was estimated to be in the range of 0.5 ngμg^-1 leaf protein. SAP12 protein decreased under salt and cold stress. These data suggest a redox state-linked function of SAP12 in plant cells particularly under cold and salt stress.

关键词： abiotic/environmental stress cold acclimation cell signaling gene expression Arabidopsis A20 and AN1 Zinc finger domains redox regulation stress associated protein thioredoxin.

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Zinc in plants:Integrating homeostasis and biofortification

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molecular Plant 2022年第1期15卷 65-85页

作者： Camilla Stanton Dale Sanders Ute Krämer Dorina Podar John Innes Centre Colney LaneNorwichNR47UHUK molecular Genetics and Physiology of plants Ruhr University Bochum44801 BochumGermany Department of molecular Biology and Biotechnology and Centre for Systems Biology Biodiversity and BioresourcesBabes-Bolyai University400084 Cluj-NapocaRomania

Zinc plays many essential roles in *** a strong Lewis acid that lacks redox activity under environ-mental and cellular conditions,the Zn2+cation is central in determining protein structure and catalytic function of nearly 10%of most eukaryotic *** specific functions of zinc have been elucidated at a molecular level in a number of plant proteins,wider issues abound with respect to the acquisition and distribution of zinc by *** important challenge is to understand how plants balance between Zn supply in soil and their own nutritional requirement for zinc,particularly where edaphic factors lead to a lack of bioavailable zinc or,conversely,an excess of zinc that bears a major risk of *** are the ultimate source of zinc in the human diet,and human Zn deficiency accounts for over 400000 deaths ***,we review the current understanding of zinc homeostasis in plants from the molecular and physiological *** provide an overview of approaches pursued so far in Zn biofortification of ***,we outline a"push-pull"model of zinc nutrition in plants as a simplifying *** summary,this review discusses avenues that can potentially deliver wider bene-fits for both plant and human Zn nutrition.

关键词： zinc biofortification Zn homeostasis cereals Arabidopsis barley wheat rice

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Genotype Screening of Recipient Resources with High Regeneration and Transformation Efficiency in Chrysanthemum

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Phyton-International Journal of Experimental Botany 2022年第4期91卷 869-888页

作者： Yajun Li Yumeng Cui Bingjie Wang Yue Li Mengmeng Zhang Silan Dai He Huang Beijing Advanced Innovation Center for Tree Breeding by molecular Design Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular BreedingNational Engineering Research Center for FloricultureBeijing Laboratory of Urban and Rural Ecological EnvironmentKey Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of EducationMinistrySchool of Landscape ArchitectureBeijing Forestry UniversityBeijing100083China Beijing Botanical Garden Beijing100093China

Genetic transformation is one of the key steps in the molecular breeding of chrysanthemum,which relies on an optimal regeneration and transformation ***,the regeneration system of different chrysanthemum cultivars varies,and the regeneration time of most cultivars is *** screen cultivars with highly efficient regeneration,leaves and shoot tip thin cell layers(tTCL)from eight chrysanthemum cultivars with different flower colors and flower types were cultured on Murashige and Skoog media(MS)supplemented with 1.0-5.0 mg L^(−1)6-benzylaminopurine(6-BA)and 0.1-1.0 mg L^(−1)α-naphthaleneacetic(NAA).The results showed that the most efficient regeneration media were MS+6-BA 1.0 mg L^(−1)+NAA 0.5 mg L^(−1)for leaf explants and MS+6-BA 5.0 mg L^(−1)+NAA 0.1 mg L^(−1)for tTCL ***,another 13 chrysanthemum cultivars were screened by using the media,and finally,three cultivars with high regeneration efficiency were obtained from 21 *** these,C1 had the highest regeneration efficiency:the regeneration rate of leaf explants reached 80.0%after 42 days of culture,and the regeneration rate of tTCL explants reached 100%after 31 days of ***,we also established the transformation system for C1 as follows:preculturing for one day,infecting with Agrobacterium suspension(OD_(600)=0.6)for 10 min,and cultivating in the regeneration medium with 350 mg L^(−1)carbenicillin and 10 mg L^(−1)kanamycin,thus ultimately achieving a transformation rate of 4.0%.In this study,a new chrysanthemum cultivar with an efficient regeneration and transformation system was screened,which is beneficial to enrich the flower color of chrysanthemum transgenic plant recipients and to the functional research of flower color or type-related genes.

关键词： Chrysanthemum tissue culture regeneration system genetic transformation cultivars screening

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Effect of low-nitrogen stress on photosynthesis and chlorophyll fluorescence characteristics of maize cultivars with different low-nitrogen tolerances

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Journal of Integrative Agriculture 2019年第6期18卷 1246-1256页

作者： WU Ya-wei LI Qiang JIN Rong CHEN Wei LIU Xiao-lin KONG Fan-lei KE Yong-pei SHI Haichun YUAN Ji-chao Key Laboratory of Crop ecophysiology and Farming System in Southwest China Ministry of Agriculture/College of AgricultureSichuan Agricultural UniversityChengdu 611130P.R.China Chongqing Key Laboratory of Economic Plant Biotechnology/Collaborative Innovation Center of Special Plant Industry in Chongqing/Institute of Special plants Chongqing University of Arts and SciencesChongqing 402160P.R.China

Nitrogen(N)is a critical element for plant growth and productivity that influences photosynthesis and chlorophyll *** investigated the effect of low-N stress on leaf photosynthesis and chlorophyll fluorescence characteristics of maize cultivars with difference in tolerance to low N *** low-N tolerant cultivar ZH311 and low-N sensitive cultivar XY508 were used as the test materials.A field experiment(with three N levels:N0,0 kg ha–1;N1,150 kg ha–1;N2,300 kg ha–1)in Jiyanyang,Sichuan Province,China,and a hydroponic experiment(with two N levels:CK,4 mmol L–1;LN,0.04 mmol L–1)in Chengdu,Sichuan Province,China were ***-N stress significantly decreased chlorophyll content and rapid light response curves of the maximum fluorescence under light(Fm′),fluorescence instable state(Fs),non-photochemical quenching(qN),the maximum efficiency of PSII photochemistry under dark-adaption(Fv/Fm),potential activity of PSII(Fv/Fo),and actual photochemical efficiency of PSII(ΦPSII)of ***,it increased the chlorophyll(Chl)a/Chl b values and so *** light compensation point of ZH311 decreased,while that of XY508 *** degree of variation of these indices in low-N tolerant cultivars was lower than that in low-N sensitive cultivars,especially at the seedling *** could increase Chl a/Chl b,apparent quantum yield and light saturation point to adapt to N *** to low-N sensitive cultivars,low-N tolerant cultivars maintained a higher net photosynthetic rate and electron transport rate to maintain stronger PSII activity,which further promoted the ability to harvest and transfer *** might be a photosynthetic mechanism by which low-N tolerant cultivar adapt to low-N stress.

关键词： maize nitrogen chlorophyll content photosynthesis chlorophyll fluorescence characteristics

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Asian Journal of Andrology 2008年第2期10卷 177-187页

作者： Khaled Hamden Dorothee Silandre Christelle Delalande Abdefattah El Feki Serge Carreau Animal ecophysiology Faculty of Sciences Sfax-3018 Tunisia USC 20061NRA- EA 2608 Biochemistry Caen-14032 France

Aim： To examine the effects on rat aging of caloric restriction （CR1） and undernutrition （CR2） on the body and on testicular weights, on two enzymatic antioxidants （superoxide dismutase and catalase）, on lipid peroxidation and on the expression of testicular aromatase and estrogen receptors （ER）. Methods： CR was initiated in 1-month-old rats and carried on until the age of 18 months. Results： In control and CR2 rats an age-related decrease of the aromatase and of ER （α and β） gene expression was observed; in parallel a diminution of testicular weights, and of the total number and motility of epididymal spermatozo was recorded. In addition, aging in control and CR2 rats was accompartied by a significant decrease in testicular superoxide dismutase, catalase activities, and an increase in lipid peroxidation level （thiobarbituric acid reactive substance）, associated with alterations of spermatogenesis. Conversely, caloric restriction-treatment exerted a protective effect and all the parameters were less affected by aging. Conclusion： These results indicate that during aging, a low caloric diet （not undernutrition） is beneficial for spermatogenesis and likely improves the protection of the cells via an increase of the cellular antioxidant defense system in which aromatase/ ER could play a role. （Asian J Andro12008 Mar; 10： 177-187）

关键词： aging low caloric diet rat testis aromatase estrogen receptors antioxidants

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Tobacco Rattle Virus-induced Phytoene Desaturase (PDS) Silencing in Centaurea cyanus

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Horticultural Plant Journal 2021年第2期7卷 159-166页

作者： Chengyan Deng Fan Zhang Jiaying Wang Yanfei Li He Huang Silan Dai Beijing Key Laboratory of Ornamental plants Germplasm Innovation&molecular Breeding National Engineering Research Center for FloricultureBeijing Laboratory of Urban and Rural Ecological Environment and College of Landscape ArchitectureBeijing Advanced Innovation Center for Tree Breeding by Molecular DesignBeijing Forestry UniversityBeijing 100083China

Virus-induced gene silencing(VIGS)is a genetic tool used to assess gene *** rattle virus(TRV)is a VIGS vector commonly used to induce endogenous gene silencing in ***,there is no VIGS system established for Centaurea *** evaluated the effectiveness of a TRV-based VIGS system using phytoene desaturase(PDS)as a reporter gene in Centaurea *** methods including pressure-,vacuum-and apical meristem-infiltrationwere tested to infect *** *** leaveswere only obtained using apicalmeristem-infiltration after a 14 d *** CcPDS transcripts in photobleached leaves were significantly reduced compared with that in green leaves treated with empty *** *** cultivars were tested to detect their VIGS responses,and‘Dwarf Tom Pouce Blue’was the most *** agro-infiltration condition was optimized by screening for the optimal seedling stage as well as the optimum Agrobacterium density for efficient *** with four true leaves and infiltration with an Agrobacterium density of OD_(600)0.5 were optimal conditions to obtain more photobleached leaves and more intense photobleached *** results demonstrated the feasibility of TRV-based VIGS for functional analysis of genes in ***.

关键词： Centaurea cyanus VIGS tobacco rattle virus PDS seedling photobleached

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