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The Clock Protein CCA1 and the bZIP Transcription Factor HY5 Physically Interact to Regulate Gene Expression in Arabidopsis

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molecular Plant 2008年第1期1卷 58-67页

作者： Christos Andronis Simon Barak Stephen M. Knowles Shoji Sugano Elaine M. Tobin Department of molecular Cell and Developmental Biology University of California Los Angeles CA 90095-1606 USA

The circadian clock regulates the expression of an array of Arabidopsis genes such as those encoding the LIGHT-HARVESTING CHLOROPHYLL A/B （Lhcb） proteins. We have previously studied the promoters of two of these Arabidopsis genes-Lhcb1＊1 and Lhcb1＊3m-and identified a sequence that binds the clock protein CIRCADIAN CLOCK ASSOCIATED 1 （CCA1）. This sequence, designated CCAl-binding site （CBS）, is necessary for phytochrome and circadian responsiveness of these genes. In close proximity to this sequence, there exists a G-box core element that has been shown to bind the bZIP transcription factor HY5 in other light-regulated plant promoters. In the present study, we examined the importance of the interaction of transcription factors binding the CBS and the G-box core element in the control of normal circadian rhythmic expression of Lhcb genes. Our results show that HY5 is able to specifically bind the G-box element in the Lhcb promoters and that CCA1 can alter the binding activity of HY5. We further show that CCA1 and HY5 can physically interact and that they can act synergistically on transcription in a yeast reporter gene assay. An absence of HY5 leads to a shorter period of Lhcbl＊l circadian expression but does not affect the circadian expression of CATALASE3 （CAT3）, whose promoter lacks a G-box element. Our results suggest that interaction of the HY5 and CCA1 proteins on Lhcb promoters is necessary for normal circadian expression of the Lhcb genes.

关键词： Biorhythms Basic-Leucine Zipper Transcription Factors Genes Transcription Factors Arabidopsis CCA1 gene CLOCK Proteins

Distinct Processing of lncRNAs Contributes to Non-conserved Functions in Stem cells

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Bulletin of the Chinese Academy of Sciences 2020年第2期34卷 116-117页

作者： CHEN Lingling Center for Excellenee in molecular cell Science Shanghai Institute of Biochemistry and cell biology CAS

Long noncoding RNAs(lncRNAs),which are longer than 200 nucleotides in length and lack protein coding potential,are pervasively transcribed in eukaryotic *** is well established that lncRNAs play important roles in gen... 详细信息

关键词： lncRNA conserved diverse

p53:not just a tumor suppressor

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Journal of molecular cell biology 2019年第7期11卷 539-543页

作者： Moshe Oren Department of molecular cell biology Weizmann Institute of ScienceRehovot 76100Israel

Nowadays,p53 is like a Dreamliner airplane,riding the high skies of cancer research with dignity and *** do not have to be a card-bearing p53 researcher to know about p53,care about it,and respect ***,this was not always *** a matter of fact,in the first years of p53 research,from its discovery in the late 1970's to about the end of the 1980's,p53 was more like a kite:one moment high up in the air,the next moment being dragged on the ground.

关键词： Dreamliner dignity ground

DICER1 regulates antibacterial function of epididymis by modulating transcription of p-defensins

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Journal of molecular cell biology 2019年第5期11卷 408-420页

作者： Chunhua Tang Minjie Ni Shengsong Xie Yao Zhang Chaobao Zhang Zimei Ni Chen Chu Ligang Wu Yuchuan Zhou Yonglian Zhang State Key Laboratory of molecular biology Shanghai Key Laboratory of Molecular Andrology CAS Center for Excellence in Molecular Cell Science Shanghai Institute of Biochemistry and Cell Biology Chinese Academy of Sciences University of Chinese Academy of Sciences Shanghai 200031 China

DICER1 is a key enzyme responsible for the maturation of microRNAs. Recent evidences suggested that DICER1 and microRNAs expressed in epididymis were involved in the control of male fertility. However, the exact mechanism remains to be elucidated. Here, we created a mouse line by targeted disruption of Dicerl gene in the principal cells of distal caput epididymis. Our data indicated that a set of β-defensin genes were downregulated by DICER1 rather than by microRNAs. Moreover, DICER1 was significantly enriched in the promoter of β-defensin gene and controlled transcription. Besides, the antibacterial ability of the adult epididymis significantly declined upon Dicerl deletion both in vitro and in vivo. And a higher incidence of reproductive defect was observed in middle-aged Dicerl^-|- males. These results suggest that DICER1 plays an important role in transcription of β-defensin genes, which are associated with the natural antibacterial properties in a microRNA-independent manner, and further impacts the male fertility.

关键词： epididymis antibacterial property DICER1 β-defensin

Msx homeobox gene family and craniofacial development

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cell Research 2003年第6期13卷 429-442页

作者： SYLVIA ALAPPAT Division of Developmental biology Department of cell and molecular biology Tulane University New Orleans LA 70118 USA.

Vertebrate Msx genes are unlinked, homeobox-containing genes that bear homology to the Drosophila muscle segment homeobox gene. These genes are expressed at multiple sites of tissue-tissue interactions during vertebrate embryonic development. Inductive interactions mediated by the Msx genes are essential for normal craniofacial, limb and ectodermal organ morphogenesis, and are also essential to survival in mice, as manifested by the pheno typic abnormalities shown in knockout mice and in humans. This review summarizes studies on the expression, regulation, and functional analysis of Msx genes that bear relevance to craniofacial development in humans and mice.

关键词： Msx genes craniofacial tooth cleft palate suture development transcription factor signaling molecule.

The universality and biological significance of signal molecules with intracellular-extracellular compatible functions

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Chinese Science Bulletin 2000年第19期45卷 1729-1734页

作者： SUN DayeInstitute of molecular cell biology, Hebei Normal University, Shijiazhuang 050016, China Institute of molecular cell biology Hebei Normal University Shijiazhuang China

Generally, cell signal molecules are classified into the extracellular signal molecules (the first messengers) and the intracellular signal ones (the second messengers). Cyclic adenosine monophosphate (cAMP), calcium ions and calmodulin (CaM) are the traditional intracellular messengers, but they are also present in extracellular matrix (ECM). Some of them have been discovered to act as the first messengers through cell surface receptors. Other second messengers, such as cyclic guanosine monophosphate (cGMP), cyclic adenosine diphosphate ribose (cADPR) and annexin, are also found existing outside animal and plant cells. The existence of these messengers with intracellular-extracellular compatible functions in cells may be a regular biological phenomenon. These compatible messengers might be the communication factors between intracellular and extracellular regions or among the cell populations, and are also important in regulating cell development procedure.

关键词： intracellular signal extracellular signal compatible messenger.

Retinal ganglion cell neuroprotection by growth factors and exosomes:lessons from mesenchymal stem cells

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Neural Regeneration Research 2018年第2期13卷 228-229页

作者： Ben Mead Stanislav Tomarev Section of retinal ganglion cell biology laboratory of retinal cell and molecular biologynational eye institutenational institutes of healthBethesdaMDUSA

Retinal ganglions cells （RGCs） are responsible for propagating electrochemical information from the eye to the brain along their axons which make up the optic nerve. The loss of RGCs is characteristic in several conditions such as glaucoma and trau- matic optic neuropathy and leads to visual loss and blindness. While no therapy exists to directly treat RGCs, the use of bone marrow-derived mesenchymal stem cells （BMSCs） has shown promise in eliciting significant RGC neuroprotection.

关键词： BMSCs Retinal ganglion cell neuroprotection by growth factors and exosomes lessons from mesenchymal stem cells PDGFR

Role of CaECM25 in cell morphogenesis,cell growth and virulence in Candida albicans

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Science China(Life Sciences) 2008年第4期51卷 362-372页

作者： ZHANG TingTing LI WanJie LI Di WANG Yue SANG JianLi Key laboratory of cell Proliferation and Regulation of Ministry of Education Beijing 100875China Candida albicans molecular and cell biology Laboratory Institute of Molecular and Cell BiologyProteos61 Biopolis DriveSin-gapore 138673

Candida albicans is the most prominent opportunistic fungal pathogen in humans. Multiple factors are associated with the virulence of C. albicans, including morphogenesis, cell wall organization and growth rate. Here, we describe the identification and functional characterization of CaECM25, a gene that has not been reported before. We constructed Caecm25?/? mutants and investigated the role of the gene in morphogenesis, cell wall organization and virulence. CaECM25 deletion resulted in defects in cell separation, a slower growth rate, reduced filamentous growth and attenuated adherence to plastic surfaces. The Caecm25?/? mutant was also significantly less virulent than wild type when tested for systemic infection in mice. Therefore, CaECM25 plays important roles in morphogenesis, cell wall organization and virulence.

关键词： Candida albicans CaECM25 gene knockout morphogenesis virulence

Induction of mitochondrion-mediated apoptosis of CHO cells by tripchloro lide

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cell Research 2003年第4期13卷 295-300页

作者： YAN REN, LEI XIONG, JIA RUI WULaboratory of molecular cell biology, Institute of Biochemistry and cell biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China 1Laboratory of molecular cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yue Yang Road Shanghai 200031 China 1Laboratory of molecular cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yue Yang Road Shanghai 200031 China 1Laboratory of molecular cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yue Yang Road Shanghai 200031 China

Tripchlorolide (TC) is a potent antitumor reagent purified from a Chinese herb Tripterygium Wilfordii Hook. f.. However, its cellular effects and mechanism of action are unknown. We showed here that TC induced apoptosis of Chinese Hamster Ovary (CHO) cells in time- and dose-dependent manners. TC resulted in the degradation of Bcl-2, the translocation of Bax from the cytosol to mitochondria, and the release of cytochrome c from mitochondria. Stable overexpression of human Bcl-2 could reduce the apoptosis of TC treated cells by blocking the translocation of Bax and the release of cytochrome c. These results indicate that TC induces apoptosis of CHO cell by activating the mitochondrion-mediated apoptotic pathway involving the proteins of Bcl-2 family and cytochrome c.

关键词： apoptosis mitochondrial pathway CHO cell.