检索结果-南通市图书馆

Pretreatment with H_2O_2 Alleviates Aluminum-induced Oxidative stress in Wheat Seedlings

Journal of Integrative Plant Biology 2011年第1期53卷 44-53页

作者： Fang Jie Xu Chong Wei Jin Wen Jing Liu Yong Song Zhang Xian Yong Lin ministry of education key laboratory of Environmental Remediation and Ecosystem Health College of Environmental & Resource Sciences Zhejiang University Hangzhou 310029 China Zhejiang key laboratory of Subtropical Soil Science and Plant Nutrition College of Environmental & Resources Science Zhejiang University Hangzhou 310029 China

Hydrogen peroxide （H2O2） is a key reactive oxygen species （ROS） in signal transduction pathways leading to activation of plant defenses against biotic and abiotic stresses. In this study, we investigated the effects of H2O2 pretreatment on aluminum （AI） induced antioxidant responses in root tips of two wheat （Triticum aestivum L.） genotypes, Yangmai-5 （AI-sensitive） and Jian-864 （AI-tolerant）. AI increased accumulation of H2O2 and O2^- leading to more predominant lipid peroxidation, programmed cell death and root elongation inhibition in Yangmai-5 than in Jian-864. However, H2O2 pretreatment alleviated AI- induced deleterious effects in both genotypes. Under AI stress, H2O2 pretreatment increased the activities of superoxide dismutase, catalase, peroxidase, ascorbate peroxidase and monodehydroascorbate reductase, glutathione reductase and glutathione peroxidase as well as the levels of ascorbate and glutathione more significantly in Yangmai-5 than in Jian-864. Furthermore, H2O2 pretreatment also in- creased the total antioxidant capacity evaluated as the 2, 2-diphenyl-l-picrylhydrazyl-radical scavenging activity and the ferric reducing/antioxidant power more significantly in Yangmai-5 than in Jian-864. Therefore, we conclude that H2O2 pretreatment improves wheat AI acclimation during subsequent AI exposure by enhancing the antioxidant defense capacity, which prevents ROS accumulation, and that the enhancement is greater in the AI-sensitive genotype than in the AI-tolerant genotype.

关键词： Peroxides Oxidation Beers Aluminium Tolerance Genotypes stress Antioxidants Glutathione reductase Roots

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Comparison of photosynthesis and antioxidative protection in Sophora moorcroftiana and Caragana maximovicziana under water stress

引用

Journal of Arid Land 2014年第5期6卷 637-645页

作者： QiQiang GUO WenHui ZHANG HuiE LI ministry of education key laboratory of Environment and Ecology in West China Northwest A&F University Tibet Agricultural and Animal Husbandry College National key Station for Field Scientific Observation&Experiment

This study aims to investigate the protective roles of photosynthetic characteristics and antioxidative systems in the desiccation tolerance of Sophora moorcroftiana and Caragana maximovicziana as they adapt to arid environments. A variety of physiological and biochemical parameters in the leaves of two Leguminosae species were monitored for 1, 7, 14, 21 and 28 d of drought stress. Soil water content decreased from 38.58% to 7.33% after exposure to 28 d of water stress. The photosynthetic carbon-assimilation rates of the two Leguminosae plants decreased for non-stomatal limitation with processing water stress. The malondialdehyde content and cell membrane relative conductivity of the two species increased significantly from 1 to 21 d and then decreased. S. moorcroftiana showed higher superoxide dismutase and peroxidase activities than C. maximovicziana during the 28 d treatment period. However, the catalase activities and proline content of C. maximovicziana were higher than those of S. moorcroftiana before the water stress treatment reached 21 d. Nine physiological and biochemical parameters were selected to comprehensively evaluate the two species＇ drought-resistance by the membership function values（MFV）. The mean MFV indicated that S. moorcroftiana has a relatively stronger drought defense capability than C. maximovicziana. S. moorcroftiana mainly uses carbon-assimilation rate and osmotic adjustment to combat water deficiency.

关键词： Sophora moorcroftiana Caragana maximovicziana photosynthesis characteristic antioxidative protection drought stress

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Potentilla anserina polysaccharide alleviates cadmium-induced oxidative stress and apoptosis of H9c2 cells by regulating the MG53-mediated RISK pathway

引用

Chinese Journal of Natural Medicines 2023年第4期21卷 279-291页

作者： ZHAO Lixia CHENG Ju LIU Di GONG Hongxia BAI Decheng SUN Wei Institute of Integrated Traditional Chinese and Western Medicine School of Basic Medical SciencesLanzhou UniversityLanzhou 730000China School of Nursing Gansu University of Chinese MedicineLanzhou 730000China key laboratory of Dunhuang Medicine Ministry of EducationGansu University of Chinese MedicineLanzhou 730000China Institute of Genetics School of Basic Medical SciencesLanzhou UniversityLanzhou 730000China key laboratory of Evidence Science Techniques Research and Application of Gansu Province Gansu University of Political Science and LawLanzhou 730000China School of Basic Medical Sciences Gansu University of Chinese MedicineLanzhou 730000China Department of Cardiac Surgery The First Hospital of Lanzhou UniversityLanzhou 730000China

Oxidative stress plays a crucial role in cadmium(Cd)-induced myocardial injury.Mitsugumin 53(MG53)and its mediated reperfusion injury salvage kinase(RISK)pathway have been demonstrated to be closely related to myocardial oxidative damage.Potentilla anserina L.polysaccharide(PAP)is a polysaccharide with antioxidant capacity,which exerts protective effect on Cd-induced damage.However,it remains unknown whether PAP can prevent and treat Cd-induced cardiomyocyte damages.The present study was desgined to explore the effect of PAP on Cd-induced damage in H9c2 cells based on MG53 and the mediated RISK pathway.For in vitro evaluation,cell viability and apoptosis rate were analyzed by CCK-8 assay and flow cytometry,respectively.Furthermore,oxidative stress was assessed by 2',7'-dichlorodihydrofluorescein diacetate(DCFH-DA)staining and using superoxide dismutase(SOD),catalase(CAT),and glutathione/oxidized glutathione(GSH/GSSG)kits.The mitochondrial function was measured by JC-10 staining and ATP detection assay.Western blot was performed to detect the expression of proteins related to MG53,the RISK pathway,and apoptosis.The results indicated that Cd increased the levels of reactive oxygen species(ROS)in H9c2 cells.Cd decreased the activities of SOD and CAT and the ratio of GSH/GSSG,resulting in decreases in cell viability and increases in apoptosis.Interestingly,PAP reversed Cd-induced oxidative stress and cell apoptosis.Meanwhile,Cd reduced the expression of MG53 in H9c2 clls and inhibited the RISK pathway,which was mediated by decreasing the ratio of p-Akt^(Ser473)/Akt,p-GSK3β^(Ser9)/GSK3β and p ERK1/2/ERK1/2.In addition,Cd impaired mitochondrial function,which involved a reduction in ATP content and mitochondrial membrane potential(MMP),and an increase in the ratio of Bax/Bcl-2,cytoplasmic cytochrome c/mitochondrial cytochrome c,and Cleaved-Caspase 3/Pro-Caspase 3.Importantly,PAP alleviated Cd-induced MG53 reduction,activated the RISK pathway,and reduced mitochondrial damage.Interestingly,knockdown of MG53 or inhibition

关键词： Potentilla anserina L. Cadmium MG53 RISK pathway Oxidative stress Apoptosis Polysaccharide

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Seasonal variation of airborne fungi of the Tiantishan Grottoes andWestern Xia Museum,Wuwei,China

引用

Research in Cold and Arid Regions 2021年第6期13卷 522-532页

作者： YuLong Duan FaSi Wu DongPeng He RuiHong Xu HuYuan Feng Tuo Chen GuangXiu Liu WanFu Wang Northwest Institute of Eco-Environment and Resources Chinese Academy of SciencesLanzhouGansu 730000China National Research Center for Conservation of Ancient Wall Paintings and Earthen Sites Dunhuang AcademyDunhuangGansu 736200China MOE key laboratory of cell activities and stress adaptations School of Life SciencesLanzhou UniversityLanzhouGansu 730000China School of Environment and Municipal Engineering Lanzhou Jiaotong UniversityLanzhouGansu 730000China Naiman Desertification Research Station Northwest Institute of Eco-Environment and ResourcesChinese Academy of SciencesTongliaoInner Mongolia 028300China

In this study,a systematic survey of cultural airborne fungi was carried out in the occurrence environments of wall paintings that are preserved in the Tiantishan Grottoes and the Western Xia Museum,China.A bio-aerosol sampler was used for sampling in four seasons in 2016.Culture-dependent and-independent methods were taken to acquire airborne fungal concentration and purified strains;by the extraction of genomic DNA,amplification of fungal ITS rRNA gene region,sequencing,and phylogenetic analysis,thereafter the fungal community composition and distribution characteristics of different study sites were clarified.We disclosure the main environmental factors which may be responsible for dynamic changes of airborne fungi at the sampling sites.The concentration of cultural airborne fungi was in a range from 13 to 1,576 CFU/m^(3),no significant difference between the two sites at the Tiantishan Grottoes,with obvious characteristics of seasonal variation,in winter and spring were higher than in summer and autumn.Also,there was a significant difference in fungal concentration between the inside and outside of the Western Xia Museum,the outside of the museum was far more than the inside of the museum in the four seasons,particularly in the winter.Eight fungal genera were detected,including Cladosporium,Penicillium,Alternaria,and Filobasidium as the dominant groups.The airborne fungal community structures of the Tiantishan Grottoes show a distinct characteristic of seasonal variation and spatial distribution.Relative humidity,temperature and seasonal rainfall influence airborne fungal distribution.Some of the isolated strains have the potential to cause biodeterioration of ancient wall paintings.This study provides supporting information for the pre-warning conservation of cultural relics that are preserved at local sites and inside museums.

关键词： airborne fungi community characteristics ancient wall paintings monitoring and pre-warning

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of extracellular vesicles from mesenchymal stem cells on oxygen-glucose deprivation/reperfusioninduced neuronal injury

引用

World Journal of Emergency Medicine 2021年第1期12卷 61-67页

作者： Shuang-shuang Gu Xiu-wen Kang Jun Wang Xiao-fang Guo Hao Sun Lei Jiang Jin-song Zhang Department of Emergency the First Affi liated Hospital of Nanjing Medical UniversityNanjing 210029China key laboratory of Modern Toxicology Ministry of EducationDepartment of ToxicologySchool of Public HealthNanjing Medical UniversityNanjing 211166China

BACKGROUND: Small extracellular vesicles (sEVs) from bone marrow mesenchymal stemcells (BMSCs) have shown therapeutic potential for cerebral ischemic diseases. However, themechanisms by which BMSC-derived sEVs (BMSC-sEVs) protect neurons against cerebral ischemia/reperfusion (I/R) injury remain unclear. In this study, we explored the neuroprotective effects ofBMSC-sEVs in the primary culture of rat cortical neurons exposed to oxygen-glucose deprivation andreperfusion (OGD/R) injury.METHODS: The primary cortical neuron OGD/R model was established to simulate the processof cerebral I/R in vitro. Based on this model, we examined whether the mechanism through whichBMSC-sEVs could rescue OGD/R-induced neuronal injury.RESULTS: BMSC-sEVs (20 μg/mL, 40 μg/mL) significantly decreased the reactive oxygenspecies (ROS) productions, and increased the activities of superoxide dismutase (SOD) and glutathioneperoxidase (GPx). Additionally, BMSC-sEVs prevented OGD/R-induced neuronal apoptosis in vivo, asindicated by increased cell viability, reduced lactate dehydrogenase (LDH) leakage, decreased terminaldeoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) staining-positivecells, down-regulated cleaved caspase-3, and up-regulated Bcl-2/Bax ratio. Furthermore, Westernblot and flow cytometry analysis indicated that BMSC-sEV treatment decreased the expression ofphosphorylated calcium/calmodulin-dependent kinase II (p-CaMK II)/CaMK II, suppressed the increaseof intracellular calcium concentration ([Ca2+]i) caused by OGD/R in neurons.CONCLUSIONS: These results demonstrate that BMSC-sEVs have signifi cant neuroprotectiveeff ects against OGD/R-induced cell injury by suppressing oxidative stress and apoptosis, and Ca2+/CaMK II signaling pathways may be involved in this process.

关键词： Oxygen-glucose deprivation and reperfusion Cortical neurons Oxidative stress Small extracellular vesicles

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

AtNHX5 and AtNHX6 are crucial for cellular pH and K+homeostasis and may function in regulating vacuolar transport in Arabidopsis

AtNHX5 and AtNHX6 are crucial for cellular pH and K+homeosta...

引用

第二届全国发育生物学大会

作者： Liguang Wang Xuexia Wu Yafen Liu Quan-Sheng Qiu MOE key laboratory of cell activities and stress adaptations School of Life SciencesLanzhou University

AtNHX5 and AtNHX6 are predicted to encode the endosomal Na/Hantiporters in Arabidopsis.Their function and underlying mechanisms remain to be studied.We found that the nhx5 nhx6 double mutant showed profound defects in growth and development.nhx5 nhx6 had smaller rosettes and shorter seedlings,and was flowering and bolting late.GUS staining shows that AtNHXS and AtNHX6 are preferentially expressed in cotyledons,hypocotyls and flowers.Both the transiently expressed Arabidopsis protoplasts and stably transformed Arabidopsis seedlings show that AtNHX5 and AtNHX6 are localized to Golgi and TGN.nhx5 nhx6 had a reduced pH,contained less Kand was sensitive to low Ktreatment,implying their crucial roles in pH and K+homeostasis.We identified three conserved acidic amino acids in the transmembrane domains that are essential for Khomeostasis and plant growth.We found that vacuolar transport was blocked in nhx5 nhx6.We further show that MVB size and numbers are alternated in nhx5 nhx6;AtNHX5 and AtNHX6 interact with the ESCRT complex subunits.Taken together,the cellular Kand Hhomeostasis maintained by the endosomal Na+,K+/H+antiporters is crucial for plant growth and development.AtNHX5 and AtNHX6 may function in regulating protein transport toward the vacuole through controlling MVB formation.

关键词： AtNHX5 AtNHX6 Na+,K+/H+ antiporters vacuolar transport Arabidopsis

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Molecular mechanisms of RLK-regulated plant cell-death control

Molecular mechanisms of RLK-regulated plant cell-death contr...

引用

细胞—生命的基础——中国细胞生物学学会2013年全国学...

作者： Junbo Du Yang Gao Yanyan Zhan Shasha Zhang Jia Li MOE key laboratory of cell activities and stress adaptations School of Life SciencesLanzhou University

Receptor-like protein kinases(RLKs) are cell surface transmembrane proteins regulating numerous cell-to-cell and cell-to-environment signaling pathways,including plant hormonal signaling and various innate immunity response pathways.BAKl and BKKl are two type II RLKs previously identified as the co-receptors of the brassinosteroid receptor BRIl.Previous studies from my lab and others demonstrated that BAKl and BKKl are actually involved in multiple developmental and defense signaling pathways,bak-4 bkk1-1 double null mutant showed a spontaneous cell death

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Efficacy of water fraction from Dioscorea cirrhosa on oxidative stress and apoptosis in H9c2 cardiomyocytes induced by H2O2

引用

Journal of Traditional Chinese Medicine 2021年第1期41卷 51-58页

作者： LIU Chunhua PAN Jie WANG Aimin LAN Yanyu LI Yongjun LU Yuan SUN Jia WANG Yonglin LIU Ting LU Dingyan YOU Jingrui State key laboratory of Functions and Applications of Medicinal Plants Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine(Ministry of Education)Guizhou Medical UniversityGuiyang 550004China Guizhou Provincial key laboratory of Pharmaceutics Guizhou Medical UniversityGuiyang 550004China School of Pharmacy Guizhou Medical UniversityGuiyang 550004China

OBJECTIVE:To investigate the efficacy of water fraction from Dioscorea cirrhosa(WF)on oxidative damage and apoptosis of cardiomyocytes induced by H2O2,and to study its mechanism.METHODS:cell viability was measured by the MST assay kit.The content of malondialdehyde(MDA),release of lactate dehydrogenase(LDH)and activity of catalase(CAT)and superoxide dismutase(SOD)were detected by biochemical kit.The content of reactive oxygen species(ROS)was assessed by nonfluorescent probe 2′,7′-dichlorofluorescin diacetate(DCFH-DA).JC-1 was used to analyze the mitochondrial membrane potential(mtΔΨ)and Annexin-V-FITC/PI staining was applied to assess apoptosis of H9c2 by flow cytometry.Moreover,the expression of B-cell lymphoma-2(Bcl-2),Bcl-2-associated X(Bax),caspase-3,caspase-9,cleaved-caspase-3 and cleaved-caspase-9 proteins was determined by western blot analysis.RESULTS:WF increased cell viability and decreased LDH leakage in H9c2 cells exposed to H2O2.WF treatment decreased ROS and MDA level,enhanced SOD and CAT activities,improved mtΔΨand inhibited apoptosis.Western blot analysis demonstrated that the ratio of Bcl-2/Bax was increased and the expression cleaved-caspase-3,caspase-3,cleaved-caspase-9 and caspase-9 were decreased in group treated with WF.CONCLUSION:WF protects H9c2 myocardial cells on H2O2-induced oxidative stress and apoptosis by scavenging ROS,improving antioxidant capacity,protecting mitochondrial and regulating the proteins expression related to apoptosis.

关键词： Dioscorea water fraction hydrogen peroxide oxidative stress apoptosis myocytes,cardiac

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Anaerobic biodegradation of trimethoprim with sulfate as an electron acceptor

引用

Frontiers of Environmental Science & Engineering 2019年第6期13卷 59-68页

作者： Bin Liang Deyong Kong Mengyuan Qi Hui Yun Zhiling Li Ke Shi E Chen Alisa SVangnai Aijie Wang Shenyang Academy of Environmental Sciences Shenyang 110167China key laboratory of Environmental Biotechnology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China State key laboratory of Urban Water Resource and Environment School of EnvironmentHarbin Institute of TechnologyHarbin 150090China ministry of education key laboratory of cell activities and stress adaptations School of Life ScienceLanzhou UniversityLanzhou 730000China The Environmental Monitoring Center of Gansu Province Lanzhou 730020China Department of Biochemistry Faculty of ScienceChulalongkorn UniversityBangkok 10330Thailand Center of Excellence on Hazardous Substance Management(HSM) Chulalongkorn UniversityBangkok 10330Thailand

Trimethoprim(TMP)is an antibiotic frequently detected in various environments.Microorganisms are the main drivers of emerging antibiotic contaminant degradation in the environment.However,the feasibility and stability of the anaerobic biodegradation of TMP with sulfate as an electron acceptor remain poorly understood.Here,TMP-degrading microbial consortia were successfully enriched from municipal activated sludge(AS)and river sediment(RS)as the initial inoculums.The acclimated consortia were capable of transforming TMP through demethylation,and the hydroxyl-substituted demethylated product(4-desmethyl-TMP)was further degraded.The biodegradation ofTMP followed a 3-parameter sigmoid kinetic model.The potential degraders(Acetobacterium,Desulfovibrio,Desulfbbulbus,and unidentified Peptococcaceae)and fermenters(Lentimicrobium and Petrimonas)were significantly enriched in the acclimated consortia.The AS-and RS-acclimated TMP-degrading consortia had similar core microbiomes.The anaerobic biodegradation ofTMP could be coupled with sulfate respiration,which gives new insights into the antibiotic fate in real environments and provides a new route for the bioremediation of antibiotic-contaminated environments.

关键词： Trimethoprim(TMP)biodegradation Demethylation Sulfate reduction Core microbiome Antibiotic fate

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

CKRC3 and NBC is required for maintain normal IAA levels in Arabidopsis

CKRC3 and NBC is required for maintain normal IAA levels in ...

引用

中国植物学会第十六次全国会员代表大会暨85周年学术年会

作者： Haiqing Liu Yuman Guo Lei Wu Peng Tian Youxia Li Junli Wang Guangqin Guo Institute of cell Biology and MOE key laboratory of cell activities and stress adaptations School of Life SciencesLanzhou University

N-terminal acetylation catalysed by N-terminal acetyltransferases(Nats) is among the most common protein post-translation modifications in eukaryotes.A typical low IAA phenotype mutant ckrc3(CK induced root curling 3) was screened by a forward genetic screen for auxin-deficient mutants by cytokinin,which show obvious curling root in 0.1 uM tZ medium.Compared with the wild type,ckrc3 exhibited shorter roots,hypocotyls,silique length,reduced root hair,decreased the plant height,and deficience of the gravitropism.And the content of both free IAA and its metabolites decreased obviously in ckrc3.The low auxin phenotype can be recovered by natural IAA and synthetic auxin(2,4-D and NAA).We identified that CKRC3 was AT5 G58450,which encodes the auxiliary non-catalytic subunit of NatB complex.Loss of function mutant nbc-1 of AT1 G03150,a gene encoding catalytic subunit of NatB,has similar phenotype to ckrc3.The results of yeast two-hybrid assays indicated that CKRC3 and NBC have strong interaction.Sub-cellular localization indicates that they co-located in the cytoplasm.These results show that CKRC3 and NBC are in the same protein complex,and may Nα-terminal acetylated auxin synthesis related proteins YUC1/2/5/6/8/9/10/11 and CYP79 B2/3,and affected auxin homeostasis.

关键词： CKRC3 NBC NatB Nα-terminal acetylation Auxin

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：