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The Identification of Phenylalanine Ammonia-Lyase(PAL)Genes from Pinus yunnanensis and an Analysis of Enzyme Activity in vitro

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Phyton-International Journal of Experimental Botany 2024年第3期93卷 503-516页

作者： Dejin Mu Lin Chen Heze Wang Zhaoliu Hu Sihui Chen Shi Chen Nianhui Cai Yulan Xu Junrong Tang Key Laboratory of National Forestry and Grassland administration on Biodiversity Conservation in Southwest China Southwest Forestry UniversityKunming650224China College of Forestry Southwest Forestry UniversityKunming650224China

Phenylalanine ammonia lyase(PAL)is the rate-limiting and pivotal enzyme of the general phenylpropanoid path-way,but few reports have been found on PAL genes in Pinus *** the present study,three PAL genes were cloned and identiﬁed from *** seedlings for theﬁrst time,namely,PyPAL-1,PyPAL-2,and *** results indicated that the open-reading frames of PyPAL genes were 2184,2157,and 2385 *** tree analysis revealed that PyPALs have high homology with other known PAL genes in other *** vitro enzymatic analysis showed that all three PyPAL recombinant proteins could catalyze the deamination of L-phenylalanine to form trans-cinnamic acid,but only PAL1 and PAL2 can catalyze the conversion of L-tyrosine toρ-coumaric *** PyPAL genes were expressed in different tissues in 1-year-old ***,and such genes had different expression *** study lays a foundation for further understanding of the biosynthesis of secondary metabolites in ***.

关键词： Pinus yunnanensis phenylalanine ammonia-lyase enzyme activity in vitro functional analysis secondary metabolites

Integrating HPWS and Organisational Memory for Enhanced Employee Performance

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Fudan Journal of the Humanities and Social Sciences 2024年第3期17卷 479-506页

作者： Moaz Gharib Kamaal Allil Department of Management College of Commerce and Business AdministrationDhofar UniversitySalalahOman Hertfordshire Business School University of HertfordshireHatfieldHertfordshireUK

This research paper investigates the relationship between high-performance work systems(HPWS),organisational memory,and employee in-role performance,focus-ing on the direct impact of HPWS on different dimensions of organisational memory and employee in-role performance,as well as the mediating role of organisational memory in this *** a sample of employees working in the tele-communications industry,the study employed structural equation modelling to ana-lyse the relationships among HPWS,organisational memory,and employee in-role *** findings revealed that HPWS positively influence all three dimen-sions of organisational memory and employee in-role performance,with organisa-tional memory partially mediating the relationship between HPWS and employee in-role ***,the study was limited to the telecommunications industry and Dhofar region and did not examine the potential moderating effects of individual-level or contextual factors on the relationships among HPWS,organisa-tional memory,and employee in-role *** study offers practical impli-cations for organizations seeking to improve employee performance and overall effectiveness by highlighting the importance of implementing high-performance work systems and managing organisational memory *** study contrib-utes to the literature on the relationships among HPWS,organisational memory,and employee in-role performance,providing valuable insights into the dynamics of organisational effectiveness.

关键词： High-performance work systems Employee in-role performance Managerial memory technical memory Cultural memory

Factors Influencing the Spatial Variability of Air Temperature Urban Heat Island Intensity in Chinese Cities

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Advances in Atmospheric Sciences 2024年第5期41卷 817-829页

作者： Heng LYU Wei WANG Keer ZHANG Chang CAO Wei XIAO Xuhui LEE Key Laboratory of Meteorological Disaster Ministry of Education(KLME)/Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters(CIC-FEMD)/Key Laboratory of Ecosystem Carbon Source and SinkChina Meteorological Administration(ECSS-CMA)/Jiangsu Key Laboratory of Agricultural MeteorologyNanjing University of Information Science&TechnologyNanjing 210044China Key Laboratory of Urban Meteorology China Meteorological AdministrationBeijing 100089China School of the Environment Yale UniversityNew HavenCT 06511USA

Few studies have investigated the spatial patterns of the air temperature urban heat island(AUHI)and its controlling *** this study,the data generated by an urban climate model were used to investigate the spatial variations of the AUHI across China and the underlying climate and ecological drivers.A total of 355 urban clusters were *** performed an attribution analysis of the AUHI to elucidate the mechanisms underlying its *** results show that the midday AUHI is negatively correlated with climate wetness(humid:0.34 K;semi-humid:0.50 K;semi-arid:0.73 K).The annual mean midnight AUHI does not show discernible spatial patterns,but is generally stronger than the midday *** urban–rural difference in convection efficiency is the largest contributor to the midday AUHI in the humid(0.32±0.09 K)and the semi-arid(0.36±0.11 K)climate *** release of anthropogenic heat from urban land is the dominant contributor to the midnight AUHI in all three climate *** rural vegetation density is the most important driver of the daytime and nighttime AUHI spatial variations.A spatial covariance analysis revealed that this vegetation influence is manifested mainly through its regulation of heat storage in rural land.

关键词： air temperature urban heat island spatial variations biophysical drivers Chinese cities climate model

Implication of machine learning techniques to forecast the electricity price and carbon emission:Evidence from a hot region

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Geoscience Frontiers 2024年第3期15卷 259-271页

作者： Suleman Sarwar Ghazala Aziz Aviral Kumar Tiwari Department of Finance and Economics College of BusinessUniversity of JeddahJeddahSaudi Arabia Department of Business administration College of Administrative and Financial SciencesSaudi Electronic UniversityJeddahSaudi Arabia Indian Institute of Management BodhgayaIndia

The current study examines the significant determinants of electricity consumption and identifies an appropriate model to forecast the electricity price *** main contribution is focused on eastern region of Saudi Arabia,a relatively hottest geographical area full of energy resources but with different electricity consumption *** relative irrelevance of temperature as predicting factor of electricity consumption is quite surprising and contradicts the previous *** the eastern region,electricity price has negative association with electricity *** comparing traditional and machine learning,it is found that machine learning techniques offer better *** the machine learning techniques,the support vector machine has the lowest errors in forecasting the electricity ***,the support vector machine approach is used to forecast the trend of carbon emissions caused by electricity *** findings have policy implications and offer valuable suggestions to policymakers while addressing the determinants of electricity consumption and forecasting electricity prices.

关键词： Electricity consumption Carbon emission Artificial neural network support vector machine Saudi Arabia

Global Zero-relaxation Limit Problem of the Electro-diffusion Model Arising in Electro-Hydrodynamics

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Acta Mathematicae Applicatae Sinica 2024年第1期40卷 241-268页

作者： Ming-hua Yang Si-ming Huang Jin-yi Sun Department of Mathematics Jiangxi University of Finance and EconomicsNanchang330032China School of Public administration Guangdong University of Foreign StudiesGuangzhou510006China College of Mathematics and Statistics Northwest Normal UniversityLanzhou730070China

In this paper,we study a global zero-relaxation limit problem of the electro-diffusion model arising in electro-hydrodynamics which is the coupled Planck-Nernst-Poisson and Navier-Stokes *** is,the paper deals with a singular limit problem of{u^(∈)_(t)+u^(∈)·▽u^(∈)-Δu^(∈)+▽P^(∈)=Δφ^(∈)▽φ^(∈),in R^(3)×(0,∞),▽·u^(∈)=0,in R^(3)×(0,∞),n^(∈)_(t)+u^(∈)·▽n^(∈)-Δn^(∈)=-▽·(n^(∈)▽φ^(∈)),in R^(3)×(0,∞),ct+u^(∈)·▽c^(∈)-Δc^(∈)=▽·(c^(∈)▽φ^(∈)),in R^(3)×(0,∞),∈^(-1)φ^(∈)_(t)=Δφ^(∈)-n^(∈)+c^(∈),in R^(3)×(0,∞),(u^(∈),n^(∈),c^(∈),φ^(∈))|t=0=(u0,n0,c0,φ0),in R^(3) involving with a positive,large parameter^(∈).The present work show a case that(u^(∈),n^(∈),c^(∈))stabilizes to(u^(∞),n∞,c∞):=(u,n,c)uniformly with respect to the time variable as^(∈)→+∞with respect to the strong topology in a certain Fourier-Herz space.

关键词： fourier-Herz space littlewood-Paley decomposition stability limit dissipative system

A comparative in vitro study on the effect of SGLT2 inhibitors on chemosensitivity to doxorubicin in MCF-7 breast cancer cells

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Oncology Research 2024年第5期32卷 817-830页

作者： SHAHID KARIM ALANOUD NAHER ALGHANMI MAHA JAMAL HUDA ALKREATHY ALAM JAMAL HIND A.ALKHATABI MOHAMMED BAZUHAIR AFTAB AHMAD Department of Clinical Pharmacology Faculty of MedicineKing Abdulaziz UniversityJeddahSaudi Arabia Department of Biochemistry Faculty of ScienceKing Abdulaziz UniversityJeddahSaudi Arabia Department of Biochemistry College of ScienceUniversity of JeddahJeddah21959Saudi Arabia health Information Technology Department The Applied CollegeKing Abdulaziz UniversityJeddahSaudi Arabia Pharmacovigilance and Medication safety Unit Center of Research Excellence for Drug Research and Pharmaceutical IndustriesKing Abdulaziz UniversityJeddahSaudi Arabia

Cancer frequently develops resistance to the majority of chemotherapy *** study aimed to examine the synergistic cytotoxic and antitumor effects of SGLT2 inhibitors,specifically Canagliflozin(CAN),Dapagliflozin(DAP),Empagliflozin(EMP),and Doxorubicin(DOX),using in vitro *** precise combination of CAN+DOX has been found to greatly enhance the cytotoxic effects of doxorubicin(DOX)in MCF-7 ***,it was shown that cancer cells exhibit an increased demand for glucose and ATP in order to support their ***,when these medications were combined with DOX,there was a considerable inhibition of glucose consumption,as well as reductions in intracellular ATP and lactate ***,this effect was found to be dependent on the dosages of the *** addition to effectively inhibiting the cell cycle,the combination of CAN+DOX induces substantial modifications in both cell cycle and apoptotic gene *** work represents the initial report on the beneficial impact of SGLT2 inhibitor medications,namely CAN,DAP,and EMP,on the responsiveness to the anticancer properties of *** underlying molecular mechanisms potentially involve the suppression of the function of SGLT2.

关键词： SGLT2 Cancer Cytotoxicity ATP Cell cycle

The Design of Multi-Connector IoT Central Gateway Based on Raspberry Pi and Docker

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Journal of Electronic Research and Application 2024年第5期8卷 66-71页

作者： Jie Wang Sichuan Vocational and technical College Suining 629000China

With the vigorous development of Internet of Things(IoT)technology,the demand for communication and data exchange between different types of IoT devices is increasing day by *** solve the problems of diversity and complexity of communication protocols between devices,this paper proposes a design scheme of a multi-connector IoT central gateway based on Raspberry Pi and *** the research and application of related technologies,by integrating multiple communication interfaces and utilizing containerization technology,an efficient,flexible,and scalable IoT central gateway has been realized,which can support the connection and data interaction of multiple communication protocols and provide strong support for the stable operation and development of the IoT system.

关键词： Internet of Things Raspberry Pi Docker Connector Central Gateway

Evaluating the Effectiveness of Consultants’ Roles in Improving Value for Money in Public Infrastructure Projects in Ghana

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Journal of Building Construction and Planning Research 2024年第3期12卷 69-85页

作者： Sheitu Abu Nongiba Alkanam Kheni Adams Abudi Department of technical and Vocational Education Mampong Technical College of Education Mampong Ghana Department of Construction Akenten Appiah-Menka University of Skills Training and Entrepreneurial Development Kumasi Ghana Department of technical and Vocational Education Bagabaga Training College Tamale Ghana

Ghanaian construction projects encounter a number of challenges, including low health, safety, and environmental requirements, poor performance, and time and cost overruns. To provide value for money (VFM) on government infrastructure projects in Ghana, this research assesses the roles of project consultants, specifically architects and quantity surveyors, and highlights important obstacles. A cross-sectional survey design involving architects and quantity surveyors yielded a 96% response rate after 100 questionnaires were distributed. Consultants’ responsibilities also include monitoring standards compliance, providing advice on delays, controlling budgets, and advising on project completion dates. Difficulties encompass a lack of promptness in decision-making, unethical conduct, political pressure, and inadequate focus on contract administration and construction audits. Project urgency, longevity, political clout, timely decision-making, and team experience are important variables that impact VFM. Policy makers and construction management practitioners should take note of the implications for Ghana’s public infrastructure projects.

关键词： Consultants Value for Money Physical Infrastructure Ghana

Pulmonary Edema and Pleural Effusion Detection Using Efficient Net-V1-B4 Architecture and AdamW Optimizer from Chest X-Rays Images

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Computers, Materials & Continua 2024年第7期80卷 1055-1073页

作者： Anas AbuKaraki Tawfi Alrawashdeh Sumaya Abusaleh Malek Zakarya Alksasbeh Bilal Alqudah Khalid Alemerien Hamzah Alshamaseen Faculty of Information Technology Al-Hussein Bin Talal UniversityMa’an71111Jordan College of Information and Communications Technology(ICT) Tafila Technical UniversityTafila66110Jordan IOTISTIC Solutions Company Amman11185Jordan

This paper presents a novelmulticlass systemdesigned to detect pleural effusion and pulmonary edema on chest Xray images,addressing the critical need for early detection in healthcare.A new comprehensive dataset was formed by combining 28,309 samples from the ChestX-ray14,PadChest,and CheXpert databases,with 10,287,6022,and 12,000 samples representing Pleural Effusion,Pulmonary Edema,and Normal cases,***,the preprocessing step involves applying the Contrast Limited Adaptive Histogram Equalization(CLAHE)method to boost the local contrast of the X-ray samples,then resizing the images to 380×380 dimensions,followed by using the data augmentation *** classification task employs a deep learning model based on the EfficientNet-V1-B4 architecture and is trained using the AdamW *** proposed multiclass system achieved an accuracy(ACC)of 98.3%,recall of 98.3%,precision of 98.7%,and F1-score of 98.7%.Moreover,the robustness of the model was revealed by the Receiver Operating Characteristic(ROC)analysis,which demonstrated an Area Under the Curve(AUC)of 1.00 for edema and normal cases and 0.99 for *** experimental results demonstrate the superiority of the proposedmulti-class system,which has the potential to assist clinicians in timely and accurate diagnosis,leading to improved patient ***,ablation-CAM visualization at the last convolutional layer portrayed further enhanced diagnostic capabilities with heat maps on X-ray images,which will aid clinicians in interpreting and localizing abnormalities more effectively.

关键词： Image classification decision support system EfficientNet-V1-B4 AdamW optimizer pulmonary edema pleural effusion chest X-rays