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Diagnosis of Bovine Tuberculosis by Comparative Intradermal Tuberculin Test, Interferon Gamma Assay and <i>esxB</i>(CFP-10) PCR in Blood and Lymph Node Aspirates

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Open Journal of Veterinary Medicine 2019年第5期9卷 55-65页

作者： Derhasar Brahma Deepti Narang Mudit Chandra Gursimran Filia Amarjit Singh Sikh Tejinder Singh Department of Veterinary microbiology College of Veterinary Science Guru Angad Dev Veterinary and Animal Sciences University Ludhiana Punjab India Department of Veterinary microbiology College of Veterinary ScienceGuru Angad Dev Veterinary and Animal Sciences University Ludhiana Punjab India Animal Disease Research Centre College of Veterinary Science Guru Angad Dev Veterinary and Animal Sciences University Ludhiana Punjab India Department of Veterinary Pathology College of Veterinary Science Guru Angad Dev Veterinary and Animal Sciences University Ludhiana Punjab India Directorate Livestock Farm College of Veterinary Science Guru Angad Dev Veterinary and Animal Sciences UniversityLudhiana Punjab India

Bovine tuberculosis (TB) is a chronic debilitating disease of huge economic importance due to loss in production, morbidity and mortality, and has a potential zoonotic threat. TB is endemic in India and has a worldwide prevalence, therefore, needing early diagnostic technique for the eradication of TB globally. Currently, compared to the eradication programme of TB in Medical sector, Veterinary sector is lagging behind though TB is one of the major zoonotic diseases prevalent in dairy animals and wildlife in India. With the “End TB” strategy by WHO in human, parallel measures for early diagnosis and culling has to be followed in case of animals for an overall successful eradication programme. The objective of this study is diagnosis of TB in cattle and buffaloes by using the cell-mediated immune response tests, i.e. Comparative Intradermal Tuberculin Test (CITT) and Interferon gamma (IFN-γ) assay, and Polymerase Chain Reaction (PCR) targeting esxB gene (CFP-10 protein) and to compare their diagnostic capabilities. This study was carried out in 202 dairy cattle and buffaloes from an organized dairy farm, where almost all of the animals appeared clinically healthy. We found that, the combined use of both CITT and IFN-γ assay lead to more accurate diagnosis of TB, although IFN-γ assay was more specific than CITT. However, esxB PCR showed almost similar sensitivity to IFN-γ assay and may be used as a fast alternative method for the diagnosis of bovine TB from blood samples.

关键词： Bovine TB CITT IFN-γ esxB PCR

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Factors Affecting Adherence to Highly Active Antiretroviral Therapy among Patients Attending Public Healthcare Facility

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Journal of Pharmacy and Pharmacology 2014年第10期2卷 600-607页

作者： Norah L. Katende-Kyenda Martie Lubbe Teke Apalata Department of Pharmacology University of Walter Sisulu Mthatha 5117 Eastern Cape Province South Africa Department of Medicine Usage in South Africa (MUSA) University of North-West Potchefstroom 4105 North-West Province South Africa Department of microbiology University of Walter Sisulu Mthatha 5117 Eastern Cape Province South Africa

The research focused on factors associated with poor adherence to HAART （highly active antiretroviral therapy） among HIV/AIDS. A descriptive cross sectional study was conducted using a standardized questionnaire and face-to-face exit interviews to collect data. Pill-counts were performed and computed adherence rate of ≥ 95% was considered acceptable. Data were analyzed using SPSS 21.0. Univariate factors associated with poor dherence to HAART were assessed with ANOVA （analysis of variance） and logistic regression model excluded confounders determining independent predictors of poor adherence. A P ≤ 0.05 was statistical significant. Of 102 HIV-infected on HAART for 24.68 ± 20.5 months, 83.3% were females and 16.7% males. The mean age （± SD） was 35.09 ± 9.3 years. Univariate factors associated with poor adherence to HAART were： CD4 count 〉 350 cells/mm3 0（2 = 46; P = 0.05）, age 〉 35 years 0（2 = 28.75; P = 0.011）, primary educational background （χ2 = 9.18; P = 0.027）, HAART regimen 1A-TDF （χ2 = 14.37; P = 0.003）, and 〉 4 combined tablets （χ2 = 11.87; P = 0.001）. There was a linear correlation between age and primary educational background （r = 0.538; P 〈 0.001）. After adjusting for univariate confounders, primary educational background （P = 0.020） and 〉 4 combined tablets （P = 0.026） were identified as independent predictors of poor adherence to HAART. Although there is an increase number of HIV-infected receiving HAART, these findings have shown that many of these will not adhere to their treatment once they improve clinically. This could be due to lack of education and complexity of combined ARVs with other drugs.

关键词： Adherence highly active antiretroviral therapy HIV/AIDS public sector.

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Intelligent Firefly Algorithm Deep Transfer Learning Based COVID-19 Monitoring System

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Computers, Materials & Continua 2023年第2期74卷 2889-2903页

作者： Mahmoud Ragab Mohammed W.Al-Rabia Sami Saeed Binyamin Ahmed A.Aldarmahi Information Technology Department Faculty of Computing and Information TechnologyKing Abdulaziz UniversityJeddah 21589Saudi Arabia Centre for Artificial Intelligence in Precision Medicines King Abdulaziz UniversityJeddah 21589Saudi Arabia Mathematics Department Faculty of ScienceAl-Azhar UniversityNaser City 11884CairoEgypt Department of Medical microbiology and Parasitology Faculty of MedicineKing Abdulaziz UniversityJeddah 21589Saudi Arabia Health Promotion Center King Abdulaziz UniversityJeddah 21589Saudi Arabia Computer and Information Technology Department The Applied CollegeKing Abdulaziz UniversityJeddah 21589Saudi Arabia Basic Science Department College of Science and Health ProfessionsKing Saud Bin Abdulaziz University for Health SciencesJeddah 21423Saudi Arabia King Abdullah International Medical Research Center Ministry of National Guard-Health AffairsJeddah 21423Saudi Arabia

With the increasing and rapid growth rate of COVID-19 cases,the healthcare scheme of several developed countries have reached the point of *** important and critical steps in fighting against COVID-19 is powerful screening of diseased patients,in such a way that positive patient can be treated and isolated.A chest radiology image-based diagnosis scheme might have several benefits over traditional *** accomplishment of artificial intelligence(AI)based techniques in automated diagnoses in the healthcare sector and rapid increase in COVID-19 cases have demanded the requirement of AI based automated diagnosis and recognition *** study develops an Intelligent Firefly Algorithm Deep Transfer Learning Based COVID-19Monitoring System(IFFA-DTLMS).The proposed IFFADTLMSmodelmajorly aims at identifying and categorizing the occurrence of COVID19 on chest *** attain this,the presented IFFA-DTLMS model primarily applies densely connected networks(DenseNet121)model to generate a collection of feature *** addition,the firefly algorithm(FFA)is applied for the hyper parameter optimization of DenseNet121 ***,autoencoder-long short term memory(AE-LSTM)model is exploited for the classification and identification of *** ensuring the enhanced performance of the IFFA-DTLMS model,a wide-ranging experiments were performed and the results are reviewed under distinctive *** experimental value reports the betterment of IFFA-DTLMS model over recent approaches.

关键词： COVID-19 artificial intelligence intelligent systems deep learning decision making

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SARS-CoV-2 spike protein and RNA dependent RNA polymerase as targets for drug and vaccine development: A review

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Biosafety and Health 2021年第5期3卷 249-263页

作者： Yusuf Muhammed Abduljalal Yusuf Nadabo Mkpouto Pius Bashiru Sani Jafar Usman Nasir Anka Garba Jaafaru Mohammed Sani Basit Opeyemi Olayanju Sunday Zeal Bala Musa Garba Abdullahi Misbahu Sambo Department of Biochemistry Federal UniversityGusauNigeria Faculty of Pharmaceutical Sciences Ahmadu Bello University ZariaNigeria Department of Medical Genetics University of CambridgeCB21TNUnited Kingdom Department of microbiology Federal University of LafiaNigeria Department of Biochemistry Kaduna State UniversityNigeria Department of Chemistry and Biochemistry Florida International UniversityFL 33199USA Department of Biochemistry Bayero University KanoNigeria Faculty of Pharmaceutical Sciences Kaduna State UniversityNigeria Department of Biochemistry Abubakar Tafawa Balewa University BauchiNigeria

The present pandemic has posed a crisis to the economy of the world and the health ***,the race to expand research to understand some good molecular targets for vaccine and therapeutic development for SARS-CoV-2 is *** newly discovered coronavirus 2019(COVID-19)is a positive sense,single-stranded RNA,and enveloped virus,assigned to the beta CoV *** virus(SARS-CoV-2)is more infectious than the previously detected coronaviruses(MERS and SARS).Findings from many studies have revealed that S protein and RdRp are good targets for drug repositioning,novel therapeutic development(antibodies and small molecule drugs),and vaccine *** such as chloroquine,convalescent plasma,monoclonal antibodies,spike binding peptides,and small molecules could alter the ability of S protein to bind to the ACE-2 receptor,and drugs such as remdesivir(targeting SARS-CoV-2 RdRp),favipir,and emetine could prevent SASR-CoV-2 RNA *** novel vaccines such as mRNA1273(Moderna),3LNP-mRNAs(Pfizer/BioNTech),and ChAdOx1-S(University of Oxford/Astra Zeneca)targeting S protein have proven to be effective in combating the present *** exploration of the potential of S protein and RdRp is crucial in fighting the present pandemic.

关键词： SARS-CoV-2 Spike protein(S protein) RNA dependent RNA polymerase(RdRp) Drug repositioning SARS-CoV-2-vaccines

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Polymorphism of mitochondrial DNA genes involved in asthenozoospermia in infertile patients of Côte d'Ivoire

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Reproductive and Developmental Medicine 2023年第1期7卷 38-43页

作者： Jules Erick Hermann Ayekoue Konan Guy Sylvère N’zi Ako Aristide Berenger Ako Marie-Florence N’guessan Yapi Guillaume Yayé FounzéguéAmadou Coulibaly Allico Joseph Djaman Biology and Health Laboratory UFR BiosciencesUniversité Felix Houphouët-Boigny22 BP 582 Abidjan 22Côte d'Ivoire Clinical and Fundamental Biochemistry Department Institut Pasteur de Côte d'Ivoire01 BP 490 Abidjan 01Côte d'Ivoire Department of Parasitology and Mycology Institut Pasteur de Côte d’Ivoire01 BP 490 Abidjan 01Côte d'Ivoire Biochemistry-microbiology Department UFR AgroforesterieUniversitéJean Lorougnon GuédéBP 150 DaloaCôte d'Ivoire

Objective:The relationship between mitochondrial DNA(mtDNA)polymorphisms and abnormalities in sperm quality has been the subject of several studies,with the objective of improving the treatment of male *** study,which contributes to the identification of genetic markers of sperm abnormalities,was conducted to study mtDNA mutations in the asthenozoospermia ***:This case-control study included 30 patients with asthenozoospermia and 28 with normospermia after spermogram and spermocytogram *** the extraction of total DNA from the spermatozoa of 58 ejaculates from these individuals using the phenol-chloroform method,the amplification of genes of interest in mtDNA using specific primers was performed by conventional polymerase chain reaction,and sequencing was used to detect ***:Male patients with asthenozoospermia in the tertiary sector had significantly more mutant-than wild-type(P=0.0005)MT-CO II ***,for the same gene,males with asthenozoospermia and primary infertility had significantly more mutants than the wild-type(P=0.001).Sequencing revealed 29 mutations that were observed only with asthenozoospermia,which could be the basis for low sperm ***:This study identified several mutations in mtDNA genes that could be considered genetic markers of asthenozoospermia if confirmed in a deeper study.

关键词： Asthenozoospermia Côte d'Ivoire Institute of Pasteur Côte d'Ivoire male infertility mtDNA polymorphism

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中国森林破碎化多尺度评价

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生物多样性 2017年第4期25卷 372-381页

作者：李瑶肖向明李香萍马俊陈帮乾秦元伟董金玮赵斌生物多样性与生态工程教育部重点实验室复旦大学生物多样性科学研究所中国上海200438 Department of microbiology and Plant Biology Center for Spatial AnalysisUniversity of OklahomaNormanOK 73019USA 中国科学院陆地表层格局与模拟重点实验室中国科学院地理科学与资源研究所中国北京100101

基于2010年50 m空间分辨率森林分布图,我们利用森林破碎化模型制作了中国森林6个不同破碎化类型(内部森林、孔洞森林、边缘森林、斑块森林、过渡森林和未确定森林)的空间分布图。然后结合2010年中国行政区划图,对比分析了不同尺度行政... 详细信息

基于2010年50 m空间分辨率森林分布图,我们利用森林破碎化模型制作了中国森林6个不同破碎化类型(内部森林、孔洞森林、边缘森林、斑块森林、过渡森林和未确定森林)的空间分布图。然后结合2010年中国行政区划图,对比分析了不同尺度行政区域的森林破碎化情况。结果表明:在国家尺度上,斑块森林的比例最大(49.05%),内部森林的比例最小(3.40%);在区域尺度上,东北地区森林破碎化程度最低,西南地区森林破碎化程度次之,华北平原、华中地区、山东半岛、黄淮海平原的森林破碎化程度最高;在省级尺度上,上海市森林破碎化程度最高,天津市次之,云南省及黑龙江省森林破碎化程度较低;在县级尺度上,陕西省所属的县森林破碎化程度最低。由此可知,总体上我国森林破碎化情况较为严重,但不同森林破碎化类型的空间分布存在较大差异。本研究可为各级林业部门管理森林资源以及优化林业生产和森林空间格局中提供数据支持,提高森林的生态系统服务功能和生物多样性保护功能。

关键词：中国森林破碎化破碎化模型多尺度比例分布

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One Health approaches adapted in low resource settings to address antimicrobial resistance

Science in One Health

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Science in One Health 2022年第1期1卷 27-33页

作者： Ripan Biswas Chanchal Debnath Samiran Bandyopadhyay Indranil Samanta Department of Veterinary Public Health F/o Veterinary and Animal SciencesWest Bengal University of Animal and Fishery Sciences37K.B.SaraniKolkata700037West BengalIndia ICAR-Indian Veterinary Research Institute Eastern Regional StationKolkataWest BengalIndia Department of Veterinary microbiology F/o Veterinary and Animal SciencesWest Bengal University of Animal and Fishery Sciences37K.B.SaraniKolkata700037West BengalIndia

Inter-disciplinary collaborations are now considered as key factors for integrated health system *** application in the domain of One Health needs more milestones to *** than the human health sector,the antimicrobials are used in food animals and aquaculture for therapy,prophylaxis and growth promotion which significantly contributes to the development of antimicrobial *** is the high time to develop a sustainable collaboration between the concerned sectors of One Health for a resilient health *** domain of One Health not only mitigates the emergence of antimicrobial resistance but also helps in realizing the surveillance and epidemiology of zoonotic diseases,and the control of public health emergencies such as *** review identified the key One Health strategies adapted by India,the exemplary low resource settings,to address antimicrobial resistance and zoonosis.

关键词： Antimicrobial resistance One Health India Agriculture,Aquaculture,Livestock Wildlife

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Multiplex Rapid Test with Acceptable Diagnosis Performance as a Solution to Increase Diagnosis of Hepatitis B and C Viruses in Pregnant Women in an Area of High Prevalence of Both Hepatitis Viruses Associated with HIV

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Open Journal of Medical microbiology 2024年第1期14卷 50-65页

作者： Catherine Boni-Cisse Nina Esther Onstira Ngoyi Hermione Dahlia Mossoro-Kpinde Noella Packo Rabi Senekian Hermann Ndoidet Koutou Coretha Bokia-Baguida Christelle Luce Bobossi-Gadia Arthur Simplice Sombot-Ndicky Daniel Yvon Gonessa Freddy Samuel Ngbonga Konzapa Voulou Christian Diamant Mossoro-Kpinde Alassane OUATTARA National Center of Medical Oncology and Radiotherapy Abidjan Ivory Cost Department of microbiology UFR of Medical Sciences University of Cocody Abidjan Ivory Cost Laboratory of Bacteriology-Virology of Teaching Hospital Center of Brazzaville Brazzaville Republic of Congo Faculty of Health Sciences Marien NGOUABI University Brazzaville Republic of Congo Department of Labor Medicine Service of HIV and STDs Control in the Workplace Bangui Central African Republic (CAR) Faculty of Health Sciences University of Bangui Bangui Central African Republic National Reference Center of Sexually Transmitted Diseases and Antiretroviral Therapy Bangui Central African Republic National Laboratory of Clinical Biology and Public Health Bangui Central African Republic Medicine Department District Hospital of Alindao Alindao Ouaka Prefecture Central African Republic Doctoral School of Sciences and Technology University of Bangui Bangui Central African Republic Coordination of Specialized Study of Clinical Biology University of Bangui Bangui Central African Republic Doctors without Borders Hospital Sica BanguiCRA Laboratory of Medical Analysis of Teaching Hospital Center Mother Elisabeth DOMITIEN of Bimbo Bimbo Central African Republic

Background and Objective: HIV, hepatitis B virus (HBV) and hepatitis C virus (HCV) are very widespread in the world, however, less than 20% of the people affected are diagnosed and treated. This study aimed to determine the prevalence of HIV, HCV and HBV co-infections in pregnant women at Bangui Community University Hospital and the cost of screening. Methods: A cross-sectional study involving consenting pregnant women who came for antenatal care was performed. HIV, HCV antibodies and HBV antigens were detected using Exacto Triplex? HIV/HCV/HBsAg rapid test, cross-validated by ELISA tests. Sociodemographic and professional data, the modes of transmission and prevention of HIV and both hepatitis viruses were collected in a standard sheet and analyzed using the Epi-Info software version 7. Results: Pregnant women aged 15 to 24 were the most affected (45.3%);high school girls (46.0%), and pregnant women living in cohabitation (65.3%) were the most represented. Twenty-five (16.7%) worked in the formal sector, 12.7% were unemployed housewives and the remainder in the informal sector. The prevalence of HIV, HBV, and HCV viruses was 11.8%, 21.9% and 22.2%, respectively. The prevalence of co-infections was 8.6% for HIV-HBV, 10.2% for HIV-HCV, 14.7% for HBV-HCV and 6.5% for HIV-HBV-HCV. All positive results and 10% of negative results by the rapid test were confirmed by ELISA tests. The serology of the three viruses costs 39,000 FCFA (60 Euros) by ELISA compared to 10,000 FCFA (15.00 Euros) with Exacto Triplex? HIV/HCV/AgHBs (BioSynex, Strasbourg, France). Conclusion: The low level of education and awareness of hepatitis are barriers to development and indicate the importance of improving the literacy rate of women in the Central African Republic (CAR). Likewise, the high prevalence of the three viruses shows the need for the urgent establishment of a national program to combat viral hepatitis in the CAR.

关键词： HIV-HCV-HBV Co-Infection Multiplex Immunochromatographic Rapid Test Central Africa Serology Test Cost Diagnostic Accessibility

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