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Photoinduced palladium-catalyzed 1,3-diene-selective fluoroalkylamination compounds as potential bactericidal agent against Xanthomonas oryzae ***

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Chinese Chemical Letters 2024年第1期35卷 401-405页

作者： Yu Shi Zhao-Sheng Zhang Jiang Shao Chen Fu Lan-Tu Xiong Zhao-Dong Li Zi-Ning Cui National Key Laboratory of Green Pesticide Integrative Microbiology Research CentreGuangdong Province Key Laboratory of Microbial Signals and Disease ControlCollege of Plant ProtectionSouth China Agricultural UniversityGuangzhou 510642China Henry Fok School of Biology and Agriculture Shaoguan UniversityShaoguan 512005China College of Materials and Energy South China Agricultural UniversityGuangzhou 510642China

A series of photoinduced palladium-catalyzed 1,3-diene-selective fluoroalkylamination derivatives was rationally synthesized based on diversity-oriented synthesis via cross coupling of 1,3-dienes,amines and fluoroalkyl *** reaction featured good function group tolerance and a broad substrate scope,which could be extended to the late-stage modification of bioactive *** activity of all the compounds against Xanthomonas oryzae ***(Xoo)was *** them,compound E14 showed significant activity against Xanthomonas oryzae ***(Xoo)with half maximal effective concentration(EC50)value of 6.61μmol/*** pot experiments,the results showed that E14 could control rice bacterial blight with protective and curative efficiencies of 37.5%and 63.2%at 200μg/mL,***,a plausible mechanism for antibacterial behavior of E14 was proposed by electron microscopy,flow cytometry,reactive oxygen species detection,and biofilm *** current work,it can promote the development of photoinduced palladium-catalyzed 1,3-diene-selective fluoroalkyl amination compounds as prospective antibacterial agent bearing an intriguing mode of action.

关键词： 1,3-Diene-selective fluoroalkylamination derivatives Xanthomonas oryzae pv.oryzae Antibacterial activity Electron microscopy Reactive oxygen Flow cytometry

Important amino acid in the hemagglutinin glycoprotein of measles virus(MV)that governs hemadsorption

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Chinese Science Bulletin 1999年第7期44卷 612-617页

作者： Michiko Watanabe Shigeharu Ueda Institute of microbial Diseases Osaka University Osaka 565Institute of Microbial Diseases Osaka University Osaka 565 JapanJapan

Two strains of measles virus IMA and SMD that were isolated using B95a cell line show hemadsorption negative. After adaptation to Vero cells, these strains gain the ability to agglutinate AGM-RBC and show hemadsorption positive. Sequence analysis of these two kinds of virus reveals that the two strains all have a Ser (HAD negative) to Gly (HAD positive) mutation at position 546 in the H protein. Site-directed mutagenesis and expression in COS cells were used to confirm that the mutation Ser→Gly is responsible for hemadsorption alteration. Then the monoclonal antibody against CD46 was used to identify that this mutation also governs the binding function of MV H protein to CD46 receptor. The data provide a new important amino acid of MV H protein that governs the hemadsorption and binding to CD46 receptor.

关键词： H protein CD46 receptor hemadsorption site-directed mutagenesis.

Tri-milR29 from Trichoderma breve Targets Chitin Synthase Gene in Botrytis cinerea and its Enhanced Disease Control Efficacy through Nanodelievery-base Application

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Tri-milR29 from Trichoderma breve Targets Chitin Synthase Ge...

Ubiquitin-Specific Protease 14 （UBP14） Is Involved in Root Responses to Phosphate Deficiency in Arabidopsis

中国植物病理学会2024年学术年会

作者：刘震 Raja Asad Ali Khan WANG Rui LI Yuejiao 刘铜 School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication) Hainan University Key Laboratory of Green Prevention and Control of Tropical Diseases and Pests Ministry of Education (School of Tropical Agriculture and Forestry)Hainan University Engineering Center of Agricultural microbial Preparation Research and Development of Hainan (Hainan University)

In the process of mycoparasitism,a form of biological control that one fungus attacking another fungus,the exchange of biologically active molecules such as RNAs may occur,playing an important role in *** these molecules,microRNAs(miRNAs) can inhibit translation and regulate gene expression at the post-transcriptional level through complementary base pairing,thereby degrading target mRNAs to prevent ***,a well-known mycoparasitic fungus,is one of the most studied biocontrol *** recent years,small RNAs(named milRNA) have also been discovered in ***,the role of Trichoderma RNAs in their mycoparasitic activity and their targets in pathogenic fungi still needs further *** study verified the cross-species regulatory role of Trichoderma-T069 miRNA,named *** demonstrated that Tri-milR29 from T069 was transferred to ***,a pathogenic fungus,where it targeted the chitin synthase gene,BcCHS7,resulting in a marked decrease in its ***-species transportation of sRNAs may occur through extracellular vesicles(EVs) ***,more research is needed on EVs in *** a series of experiments,we verified that the Tri-milR29 from T069 was transferred to *** through the EVs *** sRNA can effectively prevent and control plant diseases by targeting specific genes in pathogenic microbes through RNA ***,their susceptibility to nuclease degradation,off-target effects,and their low absorption efficiency by target pathogens limit their practical ***,the development of suitable delivery systems to protect and deliver sRNA is *** developed a carbon nanomaterial-based delivery system that enhances the stability and absorption of TrimilR29 by ***,increasing antifungal *** nano-delivered Tri-milR29 significantly lowered the disease incidence caused by *** on various fruits and tomato plants.

关键词： microRNA extracellular vesicles biocontrol mycoparasitism pathogen

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Molecular Plant 2010年第1期3卷 212-223页

作者： Wen-Feng Li Paula J. Perry Nulu N. Prafulla Wolfgang Schmidt Institute of Plant and microbial Biology Academia Sinica 115 Taipei

A mutant isolated from a screen of EMS-mutagenized Arabidopsis lines, per1, showed normal root hair development under control conditions but displayed an inhibited root hair elongation phenotype upon Pi deficiency. Additionally, the per1 mutant exhibited a pleiotropic phenotype under control conditions, resembling Pi-deficient plants in several aspects. Inhibition of root hair elongation upon growth on low Pi media was reverted by treatment with the Pi analog phosphite, suggesting that the mutant phenotype is not caused by a lack of Pi. Reciprocal grafting experiments revealed that the mutant rootstock is sufficient to cause the phenotype. Complementation analyses showed that the PER1 gene encodes an ubiquitin-specific protease, UBP14. The mutation caused a synonymous substitution in the 12th exon of this gene, resulting in a lower abundance of the UBP14 protein, probably as a consequence of reduced translation efficiency. Transcriptional profiling of per1 and wild-type plants subjected to short-term Pi starvation revealed genes that may be important for the signaling of Pi deficiency. We conclude that UBP14 function is crucial for adapting root development to the prevailing local availability of phosphate.

关键词： Phosphate deficiency root hairs ubiquitin-specific protease root development.

Physicochemical and elemental studies of Hydrocotyle javanica Thunb. for standardization as herbal drug

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Asian Pacific Journal of Tropical Biomedicine 2017年第11期7卷 979-986页

作者： Manab Mandal Debabrata Misra Narendra Nath Ghosh Vivekananda Mandal Plant and microbial physiology and Biochemistry Laboratory Department of BotanyUniversity of Gour BangaMalda 732 103West BengalIndia Department of Chemistry University of Gour BangaMalda 732 103West BengalIndia

Objective: To explore the leaves of Hydrocotyle javanica Thunb. as a source of safe and effective antibacterial herbal ***: The standardization was validated by stepwise physicochemical studies,element analysis, determination of ash values, fluorescence analysis, assessment of moisture content, extractive values in different solvent systems and extraction *** metal contents, mineral and element contents were analysed by atomic absorption spectrophotometry, inductively coupled plasma-mass spectrometer and CHNS/O analyser, ***: The methanol extract of the folklore medicinal plant having antibacterial efficacy contained flavonoids and phenolic OH groups. The ICP multi standard indicated the presence of three major compounds with molecular mass of 161 190 and 221 Da. Heavy metals viz. lead, mercury and copper content were 4.38 ppm, < 0.05 ppm and 24.70 ppm,respectively. Minerals content of calcium, phosphorus, potassium and iron were 1 190.94 mg/100 g, 375.57 mg/100 g, 2 820 mg/100 g and 340.20 mg/100 g of plant sample, respectively. Elements like carbon, hydrogen, nitrogen and sulphur contents were38.18%, 5.67%, 2.23% and 0.51%, respectively. Heavy metal profile of the tested plant was within the permissible limits of the regulatory ***: Hence the present physicochemical and elements studies reveals that the plant Hydrocotyle javanica Thunb. could be a potent source of herbal preparation as well as a safe and novel synthetic antibacterial drug.

关键词： Hydrocotyle javanica Thunb Herbal drug Physico-chemical analysis Mineral and elemental compositions Heavy metals contents Biomedical significance

A buried Neolithic paddy soil reveals loss of microbial functional diversity after modern rice cultivation

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Science Bulletin 2016年第13期61卷 1052-1060页

作者： Yong-Guan Zhu Jian-Qiang Su Zhihong Cao Kai Xue John Quensen Guang-Xia Guo Yun-Feng Yang Jizhong Zhou Hai-Yan Chu James M.Tiedje Key Laboratory of Urban Environment and Health Institute of Urban Environment Chinese Academy of Sciences State Key Laboratory of Urban and Regional Ecology Research Center for Eco-environmental Sciences Chinese Academy of Sciences Institute of Soil Science Chinese Academy of Sciences Institute for Environmental Genomics University of Oklahoma Center for microbial Ecology Department of Plant Soil and Microbial Sciences Michigan State University State Key Joint Laboratory of Environment Simulation and Pollution Control School of Environment Tsinghua University

It has been documented that human activities are causing the rapid loss of taxonomic, phylogenetic, genetic and functional diversity in soils. However, it remains unclear how modern intensive rice cultivation impacts the soil microbiome and its functionality. Here we examined the microbial composition and function differences between a buried Neolithic paddy soil and an adjacent, currently-cultivated paddy soil using high throughput metagenomics technologies. Our results showed that the currently cultivated soil contained about 10-fold more microbial biomass than the buried one. Analyses based on both 16S rRNA genes and functional gene array showed that the currently cultivated soil had significantly higher phylogenetic diversity, but less functional diversity than the buried Neolithic one. The community structures were significantly different between modern and ancient soils, with functional structure shifting towards accelerated organic carbon （C） degradation and nitrogen （N） transfor- mation in the modem soils. This study implies that, modern intensive rice cultivation has substantially altered soil microbial functional structure, leading to functional homogenization and the promotion of soil ecological functions related to the acceleration of nutrient cycling which is necessary for high crop yields.

关键词： Neolithic paddy soil Long-term ricecultivation 16S rRNA gene pyrosequencing -Bacterial community - Functional gene diversityGeoChip

A quantitative analytical method for valienone and its application in the evaluation of valienone production by a breakthrough microbial process

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Chinese Journal of Natural Medicines 2017年第10期15卷 794-800页

作者： CUI Li Karin Yanagi SHI Ting LIU Zhang-Min BAI Lin-Quan FENG Yan State Key Laboratory of microbial Metabolism School of Life Science & Biotechnology and Joint International Research Laboratory of Metabolic & Developmental Sciences Shanghai Jiao Tong University Shanghai 200240 China

Valienone is a significant natural carbasugar member of the C7-cyclitol family as a valuable precursor for glycosidase inhibitor *** is an intermediate of validamycin A biosynthesis pathway and exhibits minimal accumulation in the fermentation broth of the natural Streptomyces producer.A quantitative analytical method is crucial for the development of a breakthrough microbial process overcoming the consumption of the natural metabolic *** present study was designed to develop a pre-column derivatization high-performance liquid chromatography method for quantification of valienone and to help establish a straightforward fermentation process for valienone production by metabolically engineered Streptomyces hygroscopicus *** was derivatized by 2,4-dinitrophenylhydrazine(DNPH)in 10 mmol×L^(-1) H_3PO_4 at 37℃ for 45 min and the derivatives were separated on Eclipse XDB-C18(5 μm,4.6 mm×150 mm)column at 30 °C eluted with 50% acetonitrile for 18 *** derivatives were detected by diode array detector at 380 nm and the configurations of the derivatives were determined by computational *** method was shown to be effective,sensitive,and *** linearity was found in the range of 5–2 000 μg×mL^(-1).The intra-and inter-day precisions were 1.1%–2.7% and 1.7%–2.2%,*** absolute recovery of the spiked samples was 97.2%–102.6%.To date,this is the first reversed-phase high-performance liquid chromatography detection method for valienone in microbial culture *** method successfully helped evaluate the valienone production capability of the engineered Streptomyces hygroscopicus 5008 and could be promising for C7-cyclitol profiling of different engineered mutants combined with the metabonomics methods.

关键词： Valienone Carbasugar microbial process Derivatization HPLC

Single cell tells the developmental story

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Science Bulletin 2016年第17期61卷 1355-1357页

作者： Zhengyan Zhan Xin Xu Yu V.Fu State Key Laboratory of microbial Resources Institute of MicrobiologyChinese Academy of Sciences

Stem cells are undifferentiated cells that retain the ability to self-renew and differentiate into specialized cells,offering a promising resource to generate requisite cell types in regenerative medicine[1,2].Haematopoietic stem cells(HSCs)can differentiate into entire mature blood lineages,sitting at the apex of the haematopoietic hierachy and playing a key role in the long-term maintenance and stabilization of mammalian blood system functions[3,4].HSCs are mostly found in embryo and bone marrow,but also with small amounts in peripheral ***

关键词： maintenance differentiate specialized stabilization embryo mature story retain offering developmental

Regulation and integration of plant jasmonate signaling:a comparative view of monocot and dicot

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Journal of Genetics and Genomics 2022年第8期49卷 704-714页

作者： Shiwei Wan Xiu-Fang Xin National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200032China University of Chinese Academy of Sciences Beijing 100049China CAS-JIC Center of Excellence for Plant and microbial Sciences(CEPAMS) Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200032China

The phytohormone jasmonate plays a pivotal role in various aspects of plant life,including developmental programs and defense against pests and pathogens.A large body of knowledge on jasmonate biosynthesis,signal transduction as well as its functions in diverse plant processes has been gained in the past two *** addition,there exists extensive crosstalk between jasmonate pathway and other phytohormone pathways,such as salicylic acid(SA)and gibberellin(GA),in co-regulation of plant immune status,fine-tuning the balance of plant growth and defense,and so on,which were mostly learned from studies in the dicotyledonous model plants Arabidopsis thaliana and tomato but much less in ***,existing evidence suggests both conservation and functional divergence in terms of core components of jasmonate pathway,its biological functions and signal integration with other phytohormones,between monocot and *** this review,we summarize the current understanding on JA signal initiation,perception and regulation,and highlight the distinctive characteristics in different lineages of plants.

关键词： Jasmonate Phytohormone Signaling Crosstalk Plant defense Development Stress response