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Frontiers of Environmental Science & Engineering 2010年第4期4卷 459-465页

作者： Shuting ZHANG Bo WEI Xin YU Bing LIU Zhuoying WU Li GU Key laboratory of Urban Environment and Health Institute of Urban EnvironmentChina Academy of SciencesXiamen 361021China

The biologic activated carbon(BAC)process is widely used in drinking water treatments.A comprehensive molecular analysis of the microbial community structure provides very helpful data to improve the reactor ***,the bottleneck of deoxyribonucleic acid(DNA)extraction from BAC attached biofilm has to be solved since the conventional procedure was unsuccessful due to firm biomass attachment and adsorption capacity of the BAC *** this study,five pretreatments were compared,and adding skim milk followed by ultrasonic vibration was proven to be the optimal *** protocol was further tested using the vertical BAC samples from the full-scale biofilter of Pinghu Water *** results showed the DNAyielded a range of 40μg·g^(-1) BAC(dry weight)to over 100μg·g^(-1) BAC(dry weight),which were consistent with the biomass *** results suggested that the final protocol could produce qualified genomic DNA as a template from the BAC filter for downstream molecular biology researches.

关键词： bacterial DNA extraction biological activated carbon(BAC) biofilm water treatment pretreatment protocol

Unveiling the protective role of biofilm formation on the photoaging of microplastics

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Science China(Technological Sciences) 2024年第10期67卷 3067-3078页

作者： ZHANG HaiYu LIU Peng ZHANG JiaYu DAI JiaMin ZHANG XinRui ZHANG ZiXuan JIA HanZhong Key laboratory of Plant Nutrition and the Agri-environment in Northwest China Ministry of AgricultureCollege of Natural Resources and EnvironmentNorthwest A&F UniversityYangling 712100China Key laboratory of Organic Compound Pollution Control Engineering School of Environmental and Chemical EngineeringShanghai UniversityShanghai 200444China

In natural water,microplastics(MPs)inevitably undergo microbial colonization to form biofilm,while the effect of biofilm formation on the photoaging of MPs remains *** study systematically investigated the photoaging behavior of disposable box-derived polypropylene(PPMPs)and polystyrene(PSMPs)mediated by formed biofilm in *** incubating in Weihe water samples for 105 d,the biofilm was validated to form in PPMPs and PSMPs,with higher formation in *** particular,biofilm formation inhibited the photoaging of PPMPs and PSMPs,with an 11.1%and 50.6%decrease in photooxidation compared to their virgin counterparts after 20 d of ultraviolet(UV)***,the photoaging route of MPs such as the reaction priority of functional groups(e.g.,C–H,C=O,and benzene ring)was altered,indicating the important role of biofilm formation in the photoaging process of *** protective effect of the biofilm was mainly caused by the role of optical light filters that absorbed UV energy by sacrificing ***,as the main attacker on MPs,the production of reactive oxygen species(ROS,mainly^(1)O_(2)and·OH)was inhibited by biofilm,which was mainly responsible for the decreased photoaging of *** study revealed the important role of biofilm in the photoaging process of MPs and suggested a higher resistance of(micro)plastics in natural water than that in the laboratory using pure MPs,which contributed to a deeper understanding of the environmental fate and pollution of MPs.

关键词： microplastics biofilm photoaging physical shielding reactive oxygen species

Responses of bacterial strains isolated from drinking water environments to N-acyI-L-homoserine lactones and their analogs during biofilm formation

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Frontiers of Environmental Science & Engineering 2014年第2期8卷 205-214页

作者： Zhuoying WU1 Qing WANG, Feng GUO Shenghua ZHANG Qipei JIANG Xin YU Key laboratory of Urban Environment and Health Institute of Urban Environment Chinese Academy of Sciences Xiamen 361021 China

Often as a result ofbiofilm formation, drinking water distribution systems （DWDS） are regularly faced with the problem of microbial contamination. Quorum sensing （QS） systems play a marked role in the regulation of microbial biofilm formation; thus, inhibition of QS systems may provide a promising approach to biofilm formation control in DWDS. In the present study, 22 bacterial strains were isolated from drinking water-related environments. The following properties of the strains were investigated： bacterial biofilm formation capacity, QS signal molecule N-acyl-L-homoserine lactones （AHLs） production ability, and responses to AHLs and AHL analogs, 3-chloro-4-（dichloromethyl）-5-hydroxy-2（5H）- furanone （MX） and 2（5H）-furanone. Four AHLs were added to developed biofilms at dosages ranging from 0.1 nmol.L J to 100nmol.L1. As a result, the biofilm growth of more than 1/4 of the isolates, which included AHL producers and non-producers, were significantly promoted. Further, the biofilm biomasses were closely associated with respective AHLs concentrations. These results provided evidence to support the idea that AHLs play a definitive role in biofilm formation in many of the studied bacteria. Meanwhile, two AHLs analogs demon- strated unexpectedly minimal negative effects on biofilm formation. This suggested that, in order to find an applicable QS inhibition approach for biofilm control in DWDS, the testing and analysis of more analogs is needed.

关键词： drinking water distribution systems (DWDS) biofilm quorum sensing (QS) N-acyl-L-homoserine lac- tones (AHLs) (dichloromethyl)-5-hydroxy-2(5H)-furanone (MX) 2(5H)-furanone

Targeted Metabolomics Revealed the Regulatory Role of Manganese on Small-Molecule Metabolism of biofilm Formation in Escherichia coli

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Journal of Analysis and Testing 2020年第3期4卷 226-237页

作者： Rui Guo Haitao Lu Key laboratory of Systems Biomedicine(Ministry of Education) Shanghai Center for Systems BiomedicineShanghai Jiao Tong UniversityShanghai 200240China laboratory for Functional Metabolomics Science Shanghai Jiao Tong UniversityShanghai 200240China

biofilms are special microbial communities produced by many microorganisms,such as bacteria,viruses,and *** enable the microorganisms to possess the capacity against a diversity of stressful ***,biofilm formation often causes tough challenges in clinical infections,food quality,and environmental issues,however,the formation mechanism of biofilms are still incompletely understood which seriously impedes the development of new strategies to eradicate biofilms in different *** this study,we sought to explore the regulatory role of manganese(Mn^(2+))on small-molecule metabo-lism of biofilm formation in Escherichia coli(***).Using structural imaging assay combined with precision-targeted metabolomics method,to investigate how biofilm formation responded to various concentrations of Mn^(2+),we found that Mn^(2+)could inhibit biofilm formation through the regulation of phenotypic morphology and metabolic ***,our work discovered 16 differential functional metabolites and associated three metabolic pathways involving glycolysis,TCA cycle,and tryptophan metabolism that were changed mostly by Mn^(2+)during biofilm formation,which can differentiate biofilms from the relevant planktonic ***,this study demonstrated that Mn^(2+)can inhibit biofilm formation to regulate metabolic reprogramming and micro-structure,such effort provides novel insight into the regulation of metabolic features of biofilm formation,which enables the development of new strategies to eradicate biofilm formation for addressing the challenging problems in different areas by targeting the regulation of Mn^(2+)to the biosynthesis and expres-sions of functional metabolites produced by different microorganisms.

关键词： biofilms Manganese Precision-targeted metabolomics Imaging assay Small-molecule metabolism Escherichia coli

Wastewater treatment plants and release:The vase of Odin for emerging bacterial contaminants,resistance and determinant of environmental wellness

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Emerging Contaminants 2020年第1期6卷 212-224页

作者： Bright E.Igere Anthony I.Okoh Uchechukwu U.Nwodo Applied and Environmental Microbiology Research Group Department of Biochemistry and MicrobiologyUniversity of Fort HareAlice5700South Africa SAMRC microbial Water Quality Monitoring Centre University of Fort HareAlice5700South Africa

Municipal wastewater consists of a downstream collection of flushed sewage(without solid waste),other household runoffs,industrial runoffs,hospital runoffs and agricultural runoffs through an underground pipe before treatment.A runoff collection system called the wastewater treatment plant(WWTPs)treats such wastewater before release into environment following specific regulatory *** years-long practice has been improved upon by adding end-to-end pipe technologies with a view to enhancing the quality of effluent ***,effluents released into the environment from design/application of WWTPs appear to contain emerging contaminants of both biotic and abiotic *** observation of chemical contaminants,antibiotic resistant bacteria(ARB),antibiotic resistant genes(ARGs)and diverse pathogenic bacteria genera in wastewater works release further affirm the abundance of such emerging *** a result,the government and water regulatory organizations in various part of the world are considering the removal of water reuse act from recycling policy/*** global debate is focused on questions about sustenance of any improved additional treatment level;effect of energy consumption by added treatment stage and its impact on the environmental wellness as contaminants borne wastewater is consistently *** advancement/research suggests implementation of newer innovative infrastructural systems(NIIS)such as Mobbing Bed biofilm Rector(MBBR),for wastewater effluent management which involve addition of newer wastewater treatment *** review addressed current pitfalls including wastewater microbiota of high epidemiological/public health relevance and affirms the need for such improvement which requires modification of ongoing institutional framework with a view to encourage implementation of NIIS for an improved effluent *** the advances of microbial biofilming and the potentials of microbial biofueling as

关键词： Wastewater treatment plants(WWTPs) Municipal waste Energy Newer innovative infrastructural systems(NIIS) Wastewater effluents quality Wastewater regulatory organization Solid waste

Treatment of Aniline-Contaminated Wastewater by Sequencing biofilm Batch Reactor System Under Micro-Aerobic Condition

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Journal of Shanghai Jiaotong university(Science) 2015年第5期20卷 634-640页

作者：祁佩时陈洪一刘云芝 State Key laboratory of Urban Water Resource and Environment Harbin Institute of Technology(SKLUWREHIT)

Sequencing biofilm batch reactor(SBBR) under micro-aerobic condition was applied to the treatment of aniline-contaminated wastewater in this *** retention time(HRT) of 12—36 h and dissolved oxygen(DO) concentration of 0.1—0.5 mg/L were selected as the operating variables to model,analyze and optimize the *** dependent parameters,aniline(AN),chemical oxygen demand(COD),ammonium,total nitrogen(TN) and total phosphorus(TP) removal efficiencies as the process responses,were *** the results,increase in DO concentration could promote the AN,COD and ammonium removal;increase in HRT could also lead to increase of the AN and ammonium removal,but might decrease COD removal due to endogenous respiration and soluble microbial *** the SBBR system,24 h for HRT and 0.5 mg/L for DO concentration were chosen as the optimum operating *** actual removal efficiencies of COD,AN and ammonium under the optimum operating condition were 98.37%,100%and 89.29%,*** experimental findings were in close agreement with the model *** presence of glucose could promote bacterial growth and has positive influence on AN degradation and ammonium removal.

关键词： aniline-contaminated wastewater hydraulic retention time(HRT) dissolved oxygen(DO) microaerobic condition

Performance of low temperature microbial Fuel Cells(MFCs)catalyzed by mixed bacterial consortia

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Journal of Environmental Sciences 2017年第2期29卷 284-292页

作者： Olga Tkach Thangavel Sangeetha Spiridonova Maria Aijie Wang State Key laboratory of Urban Water Resources and Enuironment(SKLUWRE) Harbin Institute of TechnologyHarbin 150090China. Key laboratory of Environmental Biotechnology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China Krasnoyarsk State Institution of Railway Vehicles Krasnoyarsk 660028Russia

microbial Fuel Cells（MFCs） are a promising technology for treating wastewater in a sustainable manner. In potential applications, low temperatures substantially reduce MFC performance. To better understand the effect of temperature and particularly how bioanodes respond to changes in temperature, we investigated the current generation of mixed-culture and pure-culture MFCs at two low temperatures, 10°C and 5°C. The results implied that the mixed-culture MFC sustainably performed better than the pure-culture（Shewanella） MFC at 10°C, but the electrogenic activity of anodic bacteria was substantially reduced at the lower temperature of 5°C. At 10°C, the maximum output voltage generated with the mixed-culture was 540–560 m V, which was 10%–15% higher than that of Shewanella MFCs. The maximum power density reached 465.3 ± 5.8 m W/m^2 for the mixed-culture at10°C, while only 68.7 ± 3.7 m W/m^2 was achieved with the pure-culture. It was shown that the anodic biofilm of the mixed-culture MFC had a lower overpotential and resistance than the pure-culture MFC. Phylogenetic analysis disclosed the prevalence of Geobacter and Pseudomonas rather than Shewanella in the mixed-culture anodic biofilm, which mitigated the increase of resistance or overpotential at low temperatures.

关键词： microbial fuel cell Psychrophilic bacteria Shewanella Low temperature Bioelectrochemical

An antibiotic composite electrode for improving the sensitivity of electrochemically active biofilm biosensor

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Frontiers of Environmental Science & Engineering 2022年第8期16卷 97-106页

作者： Shuyi Wang Xiang Qi Yong Jiang Panpan Liu Wen Hao Jinbin Han Peng Liang State Key Joint laboratory of Environment Simulation and Pollution Control School of EnvironmentTsinghua UniversityBeijing 100084China Fujian Provincial Key laboratory of Soil Environmental Health and Regulation College of Resources and EnvironmentFujian Agriculture and Forestry UniversityFuzhou 350002China School of Ecology and Environment Zhengzhou UniversityZhengzhou 450001China

Extensive research has been carried out for improved sensitivity of electroactive biofilm-based sensor(EAB-sensor),which is recognized as a useful tool in water quality *** that is employed widely to treat infection has been proved feasible in this study to regulate the EAB and to increase the EAB-biosensor’s sensitivity.A novel composite electrode was prepared using azithromycin(AZM)and graphite powder(GP),namely AZM@GP electrode,and was employed as the anode in *** dosages of AZM,i.e.,2 mg,4 mg,and 8 mg,referred to as 0.25%,0.5%and 1%AZM@GP were under *** showed that EAB-biosensor was greatly benefited from appropriate dosage of AZM(0.5%AZM@GP)with reduced start-up time period,comparatively higher voltage output,more readable electrical signal and increased inhibition rate(30%-65%higher than control sensor with GP electrode)when exposing to toxic *** may be attributed to the fact that AZM inhibited the growth of non-EAM without much influence on the physiologic or metabolism activities of EAM under proper *** investigation of the biofilm morphology and microbial community analysis suggested that the biofilm formation was optimized with reduced thickness and enriched Geobacter with 0.5%AZM@GP *** novel electrode is easily fabricated and equipped,and therefore would be a promising way to facilitate the practical application of EAB-sensors.

关键词： AZM@GP composite electrode EAB-biosensor Water quality early-warning

Mechanistic insight into the biofilm formation and process performance of a passive aeration ditch(PAD)for decentralized wastewater treatment

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Frontiers of Environmental Science & Engineering 2022年第7期16卷 35-46页

作者： Jibin Li Jinxing Ma Li Sun Xin Liu Huaiyu Liao Di He Key laboratory for City Cluster Environmental Safety and Green Development of the Ministry of Education Institute of Environmental and Ecological EngineeringGuangdong University of TechnologyGuangzhou510006China Southern Marine Science and Engineering Guangdong laboratory(Guangzhou) Guangzhou511458China School of Environmental Science and Engineering Southern University of Science and TechnologyShenzhen518055China

Decentralized wastewater containing elevated salinity is an emerging threat to the local environment and sanitation in remote coastal *** the cost and treatment efficiencies,we propose a passive aeration ditch(PAD)using non-woven polyester fabric as a feasible bubbleless aerator and biofilm carrier for wastewater *** has been first given to PAD’s efficacy in treating saline decentralized wastewater,and then to the impact of chemical oxygen demand-to-nitrogen(C/N)ratio and salinity on biofilm formation.A multispecies model incorporating the salinity effect has been developed to depict the system performance and predict the microbial *** showed that the PAD system had great capacity for pollutants *** biofilm thickness increased at a higher C/N ratio because of the boost of aerobic heterotrophs and denitrifying bacteria,which consequently improved the COD and total nitrogen ***,this led to the deterioration of ammonia ***,while a higher salinity benefited the biofilm growth,the contaminant removal efficiencies decreased because the salinity inhibited the activity of aerobic heterotrophs and reduced the abundance of nitrifying bacteria inside the *** on the model simulation,feed water with salinity below 2%and C/N ratio in a range of 1 to 3 forms a biofilm that can reach relatively high organic matter and ammonia *** findings not only show the feasibility of PAD in treatment of saline decentralized wastewater,but also offer a systematic strategy to predict and optimize the process performance.

关键词： Decentralized wastewater Passive aeration ditch biofilm formation C/N ratio Salinity Model simulation

福氏志贺菌mdoC基因对其在洗菜水中生长及生物膜形成的影响

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西北农林科技大学学报（自然科学版） 2009年第12期37卷 175-180页

作者：刘柳樊明涛 Bhagwat A Arvind 西北农林科技大学食品科学与工程学院陕西杨凌712100 U S Department of Agriculture Agricultural Research Service Environmental microbial and Food Safety laboratory BeltsvilleMD20705USA

【目的】研究福氏志贺菌mdoC基因对细菌在不同洗菜水中生长及生物膜形成的影响。【方法】以福氏志贺菌野生型和敲除mdoC基因的opgC突变体为出发菌株,采用生长曲线法和结晶紫染色法,在低渗透压及正常渗透压的菠菜、芹菜、生菜及白菜洗菜... 详细信息

【目的】研究福氏志贺菌mdoC基因对细菌在不同洗菜水中生长及生物膜形成的影响。【方法】以福氏志贺菌野生型和敲除mdoC基因的opgC突变体为出发菌株,采用生长曲线法和结晶紫染色法,在低渗透压及正常渗透压的菠菜、芹菜、生菜及白菜洗菜水中,研究福氏志贺菌野生型和opgC突变体生长及生物膜的产生能力。【结果】在不同洗菜水中,福氏志贺菌opgC突变体生长速率明显较野生型慢,加盐提高渗透压之后,野生型和opgC突变体稳定生长期均提前。野生型和opgC突变体的生物膜产量在菠菜水、生菜水和白菜水中有显著区别;提高渗透压之后,福氏志贺菌野生型在菠菜水中的生物膜产量显著提高,而在生菜水和白菜水中生物膜产量显著下降,opgC突变体在菠菜水、生菜水和白菜水中生物膜产量均显著提高。【结论】低渗条件下,福氏志贺菌mdoC基因的缺失显著地延缓了细菌生长。提高渗透压后,在菠菜、生菜和白菜洗菜水中,mdoC基因的缺失促进了生物膜的形成。

关键词：福氏志贺菌 opgC突变体渗透调节周质葡聚糖生物膜洗菜水