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Effects of adrenomedullin gene overexpression on biological behavior of hepatic stellate cells

World Journal of Gastroenterology 2005年第23期11卷 3549-3553页

作者： YiWang Jin-ShengZhang Guang-CunHuang QiCheng Zhong-HuaZhao DepartmentofPathology MedicalCenterFudanUniversityShanghai200032China

AIM: To investigate the effects of adrenomedullin (AM) gene overexpression on the biological characteristics of human hepatic stellate cells (hHSCs) by stable ***: hHSCs which express low basal levels of AM were stably transfected with an expression construct containing rat AM gene or with an empty expression vector. Expression of AM in hHSCs was determined by reverse transcription (RT)-polymerase chain reaction (PCR) and radioimmunoassay (RIA). Cell proliferation was evaluated by 5-bromo-2'-deoxyuridine (BrdU) incorporation and immunocytochemistry. RT-PCR and Western blot were used to test the expression of procollagen types Ⅰ and Ⅲ. Protein expressions of interstitial collagenase (MMP-1), gelatinase (MMP-2) and tissue inhibitors of matrix metalloproteinases-2 (TIMP-2) were assessed by Western ***: Two cell clones (A-2, A-8) transfected withthe AM gene expressed higher levels of AM mRNA (nontransfected group: 0.86±0.11, empty vector group: 1.01±0.11, A-2 clone group: 1.44±0.08 and A-8 clone group: 1.36±0.05) and protein (12.31±0.17, 12.35±0.12,12.56±0.06 and 12.62±0.07) (P＜0.05). AM geneoverexpression had inhibitory effects on cell proliferation of hHSCs (29.6%, 30.9%, 18.9% and 21.8%, respectively. P＜0.05) and expression of procollagen type Ⅰ (0.58±0.1,0.48±0.11, 0.3±0.06 and 0.31±0.07 at mRNA level)(0.27±0.07, 0.3±0.06, 0.14±0.05 and 0.13±0.05 at protein level) (P＜0.05) and procollagen type Ⅲ (0.17±0.04, 0.15±0.03, 0.1±0.02 and 0.09±0.02 at mRNA level) (0.22±0.04, 0.2±0.03, 0.11±0.04 and 0.13±0.03 at protein level) (P＜0.05). Compared with cells non-transfected (TIMp2: 2.77±0.03, MMP-2: 0.5±0.04, MMP-1: 0.49±0.07) and transfected with empty vector (TIMP2: 2.79±0.04,MMP-2: 0.48±0.03, MMP-1: 0.45±0.09), these two clones had lower expression levels of TIMP2(A-2 clone group: 2.7±0.02 and A-8 clone group: 2.71±0.02) (P＜0.05) and MMP-2(A-2 clone group: 0.15±0.05 and A-8 clone group: 0.13±0.04) (P＜0.05) but displayed a higher expression level of

关键词： Adrenomedullin Hepatic stellate cell Procollagen type Ⅰ and Ⅲ

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Suppressing autoimmunity by TGF-β： not just through Treg cells

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Cellular & Molecular Immunology 2012年第5期9卷 371-372页

作者： Ming-Xiao He You-Wen He Department of Immunology Duke University MedicalCenter Durham NC USA

Cellular ＆ Molecular Immunology （2012） 9,371-372; doi： 10.1038/cmi.2012.24; published online 13 August 2012Transforming growth factor-β （TGF-β） is a pleiotropic factor. It regulates manyaspects of T-cell biology including thymocyte development, T cell differentiation and effector T cell function.1 Previous work has shown that the loss of TGF-β signaling in the T-cell lineage leads to severe lymphopro- liferafive autoimmunity.2.3 Until now, it has been unclear to what extent the autoimmunity observed in those mice is driven by TGF- unresponsive conventional T cells, as TGF- is also crucial for the maintenance and function of regulatory T cells （Treg）. A recent study pub- lished in Nature Immunology revealed a novel role of TGF-β signaling in non-regulatory naive T cells. It was found that TGF-β controls lym- phopenia-driven T-ceU proliferation in neonatal mice, thereby preventing the development of autoimmtmity.

关键词： T-CELLS HOMEOSTASIS MECHANISMS

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Should cross-sex hormone treatment of transsexual subjects vary with ethnic group？

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Asian Journal of Andrology 2014年第6期16卷 809-810页

作者： Louis J Gooren Department of Endocrinology VU University MedicalCenter Amsterdam the Netherlands Androconsult ChiangMai Thailand

Guidelines for cross-sex hormone treatment of transsexual people have been developed, but no attention has been paid to the specifics of ethnic groups. South East （SE） Asian male-to-female （MtoF） transsexual people may be able to transition to the female sex with lower doses of estrogens/progestins than Caucasians thus reducing health risks. Female-to-male （FtoM） may virilize less profoundly with standard doses of androgens, but this is probably sufficient to pass acceptably as men in view of the less pronounced sex differences in physique in Asians compared with Caucasians. It is timely that studies in Asians are conducted to get a better insight into their specific needs and risks of cross-sex hormone treatment.

关键词： FAT

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Retrospective analysis of old-age colitis in the Dutch inflammatory bowel disease population

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World Journal of Gastroenterology 2008年第20期14卷 3183-3187页

作者： Muhammed Hadithi Marcel Cazemier Gerrit A Meijer Elisabeth Bloemena Richel J Felt-Bersma Chris J Mulder Stephan GM Meuwissen Amado Salvador Pea Adriaan A van Bodegraven Department of Gastroenterology VUmcUniversity Medical Center Department of Pathology VUmc University MedicalCenter

AIM： To describe the characteristics of Dutch patients with *** bowel disease （IBD） first diagnosed above 60 years of age-a disease also known as old-age colitis （OAC） and to highlight a condition that has a similar appearance to IBD, namely segmen- tal colitis associated with diverticular disease （SCAD）. METHODS： A retrospective longitudinal survey of patient demographic and clinical characteristics, disease characteristics, diagnostic methods, management and course of disease was performed. The median follow-up period was 10 years. RESULTS： Of a total of 1100 IBD patients attending the Department of Gastroenterology, 59 （50） [median age 82 years （range 64-101）; 25 male （42%）] were identified. These patients were diagnosed with ulcerative colitis （n = 37, 61%）, Crohn＇s disease （n = 14, 24%）, and indeterminate colitis （n = 8, 15%）. Remission was induced in 40 （68%） patients within a median interval of 6 mo （range 1-21） and immunosuppressive therapy was well tolerated. Histological evaluation based on many biopsy samples and the course of the disease led to other diagnosis, namely SCAD instead of IBD in five （8%） patients. CONCLUSION： OAC is not an infrequent problem for the gastroenterologist, and should be considered in the evaluation of older patients with clinical features suggestive of IBD. Extra awareness and extensive biopsy sampling are required in order to avoid an erroneous diagnosis purely based on histological mimicry of changes seen in SCAD, when diagnosing IBD in the presence of diverticulosis coli.

关键词： Inflammatory bowel disease Old-age colitis Segmental colitis

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Anaerobic bacteria and intrahepatic stones: detections of Clostridium sp. and Bacteroides fragilis

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Chinese Medical Journal 2000年第9期113卷 90-93页

作者： LIU Yanlei Kan Lam Yajarayma J Tang Paul H Gumerlock Dong Ki Lee Myung Hwa Kim Sum P Lee Joseph SilvaJr Joseph W Leung Division of Gastroenterology and Mr & MrsCWLaw Biofilm Research Center University of CaliforniaDavis MedicalCenterSacramentoCA95817USA

To detect anaerobic bacteria Clostridium sp . and Bacteroides fragilis in intrahepatic stones by molecular genetic method Methods DNA was extracted from 59 stone samples and subjected to polymerase chain reaction (PCR) amplification targeting the 16S rRNA gene of Clostridium sp . and the glutamine synthetase gene of Bacteroides fragilis Single-strand conformational polymorphism (SSCP) analysis was performed to identify the Clostridium sp Results 16S rRNA gene sequences for Clostridium sp. were identified in 49 stones (83%, 49/59) The two most common groups were detected in 19 (41%) and 17 (37%) of the 46 samples using SSPC analysis, and 25/59 (42%) stones were tested positive for Bacteroides fragilis Conclusions Anaerobes such as Clostridium sp and Bacteroides fragilis present in intrahepatic stones and may play a role in stone formation PCR is a useful technique to detect fastidious pathogens, which are difficult to culture SSCP of PCR products is a rapid method in differentiating bacterial species

关键词： intrahepatic stone anaerobic bacteria PCR single-strand confromational polymorphism

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Further analysis of PREVAIL： enzalutamide use in chemotherapy-naive men with metastatic castration- resistant prostate cancer

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Asian Journal of Andrology 2014年第6期16卷 803-804页

作者： Jeanny B Aragon-Ching Department of Medicine Division of Hematology andOncology George Washington University MedicalCenter 2150 Pennsylvania Avenue NW WashingtonDC 20037 USA.

PREVAIL was a phase III multinational, double-blind, placebo-controlled trial that enrolled chemotherapy-naive men with metastatic castration-resistant prostate cancer （mCRPC）, which showed remarkable improvement in co-primary endpoints with an overall 81% reduction in the risk of radiographic progression, as well as 29% reduction in the risk of death in favor of the enzalutamide arm over placebo. All secondary endpoints including time to subsequent chemotherapy initiation and prostate specific antigen （PSA） progression were in favor of the enzalutamide arm. The results of PREVAIL shows the utility of enzalutamide that would likely soon expand the indication to asymptomatic or minimally symptomatic men with mCRPC not previously treated with chemotherapy.

关键词： ABIRATERONE DOCETAXEL

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Determining the role of metallothionein in cadmium toxicity using genetically altered mice

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中国药理学与毒理学杂志 2002年第6期16卷 401-401页

作者： KlaaC UniversityofKansas MedicalCenterUSA

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Helicobacter pylori and gastric mucin expression: A systematic review and meta-analysis

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World Journal of Gastroenterology 2015年第31期21卷 9430-9436页

作者： Yaron Niv Department of Gastroenterology Rabin MedicalCenterTel Aviv University

AIM: To investigate the relationship between Helicobacter pylori(H. pylori) and mucin expression in gastric ***: English Medical literature searches were conducted for gastric mucin expression in H. pylori infected people vs uninfected people. Searches wereperformed up to December 31 th 2014,using MEDLINE,Pub Med,EMBASE,Scopus,and CENTRAL. Studies comparing mucin expression in the gastric mucosa in patients positive and negative for H. pylori infection,were included. Meta-analysis was performed by using Comprehensive meta-analysis software(Version 3,Biostat Inc.,Englewood,NJ,United States). Pooled odds ratios(ORs) and 95% confidence intervals(CIs) were calculated compared mucin expression in individual studies by using the random effects model. Heterogeneity between studies was evaluated using the Cochran Q-test,and it was considered to be present if the Q-test P value was less than 0.10. I2 statistic was used to measure the proportion of inconsistency in individual studies,with I2 > 50% representing substantial heterogeneity. We also calculated a potential publication ***: Eleven studies,which represent 53 substudies of 15 different kinds of mucin expression,were selected according to the inclusion criteria. Every kind of mucin has been considered as one study. When a specific mucin has been studied in more than one paper,we combined the results in a nested metaanalysis of this particular mucin: MUC2,MUC6,STn,Paradoxical con A,Tn,T,Type 1 chain mucin,Le A,SLe A,Le B,AB-PAS,MUC1,and MUC5 AC. The odds ratio of mucin expression in random analysis was 2.33,95%CI: 1.230-4.411,P = 0.009,higher expression in H. pylori infected patients. Odds ratio for mucin expression in H. pylori positive patients was higher for MUC6(9.244,95%CI: 1.567-54.515,P = 0.014),and significantly lower for MUC5AC(0.447,95%CI: 0.211-0.949,P = 0.036). Thus,H. pylori infection may increase MUC6 expression and decrease MUC5 AC expression by 924% and 52%,***: H.

关键词： Helicobacter pylori Gastric mucin Stomach Secretio

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Androgen deprivation therapy for prostate cancer： not so simple

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Asian Journal of Andrology 2011年第2期13卷 187-188页

作者： Nicholas N Tadros Mark Garzotto Division of Urology Oregon Health ＆ Science UniversityPortland OR 97239 USA and 2portland VA MedicalCenter 3710 SW US Veterans Hospital Pd. PortlandOR 97239 USA

Prostate cancer （PC） is the second most diagnosed visceral malignancy in men worldwide, with over 900 000 new diagnoses each year.1 Approximately 50% of patients treated in industrialized nations will receive androgen deprivation therapy （ADT） at some point in their lifetimes.2 The use of ADT as a treatment approach is likely to increase as new drug developments have focused on intensifying the effect of reducing androgen receptor activation.

关键词： Androgens Physics Wells

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Behavioral consequences of children with sleep-disordered breathing after adenotonsillectomy

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World Journal of Pediatrics 2018年第1期14卷 57-65页

作者： Ji Yoon Kim Chang Ho Lee Hyoung-Mi Kim Otorhinolaryngology Department CHA UniversitySeongnamKorea Otorhinolaryngology Department CHA Bundang MedicalCenterCHA University351Yatap-dongBundang-guSeongnam-siGyeonggi-do463-712Korea

Background Adenotonsillectomy (AT) has been an effective treatment for sleep-disordered breathing (SDB) in children, and several studies described the risk of postoperative weight gain and obesity in children treated with AT. The present study aimed to evaluate behavioral improvements in children with SDB one year after adenotonsillectomy and to investigate an influence of postoperative weight gain on behaviors. Methods The study included 170 children aged 5–11 years who underwent adenotonsillectomy for SDB and 150 controls. Body mass index percentile was obtained for age and gender, and parental sleep-related breathing disorder (SRBD) question-naire was used to assess the severity of SDB. Psychological assessment was performed pre- and post-adenotonsillectomy using standardized questionnaires including strength and difficulties questionnaire, children's depression inventory and screen for child anxiety-related emotional disorder. Results The mean age of 170 patients was 7.7 ± 1.5 years with 73 (42.9%) girls and 97 (57.1%) boys. The mean follow-up period were 15.4 ± 2.7 months. The patients had shown significant improvements in SDB scores as well as in questionnaire-based behavioral problems after adenotonsillectomy. The odds of a child being overweight were significantly increased after adenotonsillectomy. Less improvements in hyperactivity and conduct problems were observed in the patients with older ages, higher SRBD scores, and overweight/obesity at 1-year follow-up after adenotonsillectomy. Conclusion These data suggest that abnormal behavioral outcomes should be evaluated postoperatively, which potentially could be reduced with the early adenotonsillectomy and adequate postoperative weight control.

关键词： Obesity Obstructive sleep apnea Pediatric Snoring Tonsillectomy

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