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Polymeric vs hydroxyapatite-based scaffolds on dental pulp stem cell proliferation and differentiation

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World Journal of Stem Cells 2015年第10期7卷 1215-1221页

作者： Arash Khojasteh Saeed Reza Motamedian Maryam Rezai Rad Mehrnoosh Hasan Shahriari Nasser Nadjmi Oral and Maxillofacial Surgery Dental Research CenterResearch Institute of Dental SciencesSchool of DentistryShahid Beheshti University of Medical Sciences dental Research center Research Institute of Dental SciencesSchool of DentistryShahid Beheshti University of Medical Sciences the Team for Cleft and Craniofacial Anomalies Oral and Maxillofacial SurgeryUniversity of Antwerp

AIM: To evaluate adhesion, proliferation and differentiation of human dental pulp stem cells(h DPSCs) on four commercially available scaffold biomaterials. METHODS: hD PSCs were isolated from human dental pulp tissues of extracted wisdom teeth and established in stem cell growth medium. h DPSCs at passage 3-5 were seeded on four commercially available scaffold biomaterials, SureO ss(Allograft), Cerabone(Xenograft), PLLA(Synthetic), and OSTEON Ⅱ Collagen(Composite), for 7 and 14 d in osteogenic medium. Cell adhesion and morphology to the scaffolds were evaluated by scanning electron microscopy(SEM). Cell proliferation and differentiation into osteogenic lineage were evaluated using DNA counting and alkaline phosphatase(ALP) activity assay, respectively. RESULTS: All scaffold biomaterials except Sure Oss(Allograft) supported h DPSC adhesion, proliferation and differentiation. hD PSCs seeded on PLLA(Synthetic) scaffold showed the highest cell proliferation and attachment as indicated with both SEM and DNA counting assay. Evaluating the osteogenic differentiation capability of hD PSCs on different scaffold biomaterials with ALP activity assay showed high level of ALP activity on cells cultured on PLLA(Synthetic) and OSTEON ⅡCollagen(Composite) scaffolds. SEM micrographs also showed that in the presence of Cerabone(Xenograft) and OSTEON Ⅱ Collagen(Composite) scaffolds, the h DPSCs demonstrated the fibroblastic phenotype with several cytoplasmic extension, while the cells on PLLA scaffold showed the osteoblastic-like morphology, round-like shape. CONCLUSION: PLLA scaffold supports adhesion, proliferation and osteogenic differentiation of hD PSCs. Hence, it may be useful in combination with hD PSCs for cell-based reconstructive therapy.

关键词： Human dental pulp stem cell Stem cell Tissue engin

Influence of Aging and 2-hydroxyethyl-methacrylate Content on the Color Stability of Experimental 2-hydroxyethyl-methacrylate-added dental Glass Ionomers

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Journal of Materials Science & Technology 2010年第4期26卷 379-384页

作者： Bin Yu Guangfeng Zhao Honam Lim Jinik Lira Yongkeun Lee Department of dental Materials and center for dental Materials School of Dentistry Kyung Hee University Seoul Korea Denforus co. Seaul Korea

Color stability of dental resin modified glass ionomer （RMGI） has been a challenge to dentistry; therefore, systematic changes in 2-hydroxyethyl methacrylate （HEMA） content were performed experimentally to find an idea to enhance the color stability. Changes in color （△E＊ab） and color coordinates （△L＊, △a＊ and △b＊） of experimental 10-50 wt pct HEMA-added dental glass ionomers （HAGIs） and corresponding RMGIs were determined after 5000 cycles of thermocycling. Color changes of HAGIs were not influenced by the HEMA content while △L＊, △a＊ and △b＊ values were influenced by the HEMA content. Color stability of 30% or 40% HEMA-added HAGIs was not different from those of the commercial RMGIs. Since the influence of HEMA itself on the color stability of HAGIs was limited, compositional modification to increase the color stability of these materials should be developed.

关键词： Glass ionomer 2-hydroxyethyl methacrylate (HEMA) Color coordinate Thermocycling

In-vitro bioactivity evaluation and physical properties of an epoxy-based dental sealer reinforced with synthesized fluorine-substituted hydroxyapatite, hydroxyapatite and bioactive glass nanofillers

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Bioactive Materials 2019年第1期4卷 322-333页

作者： Bahaa Abdulrazzaq Jerri Al-Bakhsh Farhad Shafiei Atieh Hashemian Kiana Shekofteh Behnam Bolhari Marjan Behroozibakhsh Department of dental Biomaterials School of DentistryTehran University of Medical SciencesTehranIran Department of Conservative Dentistry Dental School of University of BasraBasraIraq Department of dental Biomaterials School of Dentistry/Research Center for Science and Technology in MedicineTehran University of Medical SciencesTehranIran Department of Endodontic School of DentistryTehran University of Medical SciencesTehranIran

The purpose of this study was to evaluate the physical properties and bioactivity potential of epoxy-based dental sealers modified with synthesized bioactive glass(BAG),hydroxyapatite(HA)and fluorine substituted hydroxyapatite(FHA)*** synthesized powders were incorporated at 10%and 20%into the epoxy-based dental *** setting time,flow and solubility and microhardness of the modified and unmodified samples were *** bioactivity was evaluated using FESEM-EDX and elemental mapping,ATR-FTIR and *** flow value of all of the experimental groups except the FHA modified samples,was greater than 20 *** solubility,no specimens exhibited more than 1%weight *** solubility value of the FHA groups was statistically significant lower than other groups(p≤0.001).The mean hardness values of all of the modified samples were significantly higher than the unmodified group(p≤0.001).Regarding bioactivity,in vitro study revealed that after 3 days immersion in SBF a compact and continuous calcium phosphate layer formed on the surface of epoxy sealers containing BAG and HA *** on these results,the addition of BAG and HA nanoparticles did not adversely alter the physical properties of epoxy ***,they improved the in vitro bioactivity of the epoxy sealer.

关键词： Bioactivity Bioactive glass Epoxy-based dental sealer Hydroxyapatite Fluorine-substituted hydroxyapatite

Feasibility of laparoscopic total gastrectomy in overweight patients: Implications of less impact of overweight on laparoscopic versus open approach

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World Journal of Clinical Cases 2018年第16期6卷 1094-1100页

作者： Masatoshi Nakagawa Kazuyuki Kojima Mikito Inokuchi Kenta Kobayashi Toshiro Tanioka Keisuke Okuno Kentaro Gokita Department of Gastric Surgery Tokyo Medical and Dental University center for Minimally Invasive Surgery Tokyo Medical and Dental University

AIM To investigate safety and oncological feasibility of laparoscopic total gastrectomy(LTG) in overweight(OW) *** Patients who underwent total gastrectomy(110 laparoscopic, 211 open) for gastric cancer between January 1999 and July 2016 were included. Propensity score matching selected 152 patients(76 laparoscopic, 76 open), which were subsequently divided into the OW(≥ 25) or non-OW(< 25) group by body mass index. Postoperative outcomes of laparoscopic versus open approaches were compared between OW and non-OW groups. RESULTS In the propensity-matched population, baseline characteristics were comparable between the OW and nonOW groups for the laparoscopy and open groups. In the laparoscopy group, operative time was longer(P = 0.01) in the OW group, however, other perioperative results including complication rates were comparable between the non-OW and OW groups. In the open group, number of retrieved lymph nodes were less(P = 0.03) and local complication rate was more frequent(P = 0.03) in the OW group. CONCLUSION LTG in OW patients remains technically challenging but can be performed safely. Our findings imply that OW has a lesser effect on the laparoscopic versus open approach to total gastrectomy.

关键词： Gastric cancer Laparoscopy Overweight Total gastrectomy

Present status and future directions:Apexification

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World Journal of Methodology 2025年第1期15卷 26-32页

作者： Sachin Chauhan Radha Chauhan Prashant Bhasin Bhavna G Sharaf Department of Conservative Dentistry and Endodontics Sudha Rustagi College of Dental Sciences and ResearchFaridabad 121002HaryanaIndia Department of Prosthodontics and Crown and Bridge and Oral Implantology Mahatma Gandhi Dental College&HospitalJaipur 302022RajasthanIndia Department of Pedodontics and Preventive Dentistry Sudha Rustagi College of Dental Sciences and ResearchFaridabad 121002HaryanaIndia

The success rate of apexification is primarily determined by multiple factors,including the material used,the size of the open apex compared to the length of the root,and the technique used in each *** main objective of this review was to provide an update on the present management of open apex to identify factors and circumstances that may influence the success of apexification using different materials and *** research on apexification should focus on how to treat open apices with wide periapical lesions without ***,the predictability of these parameters with non-surgical procedures was uncertain,but now,with the use of a dental operating microscope,it has become more *** reason could be that extra visits are no longer required due to major advances in the armamentarium and materials used for apexification.

关键词： Bioceramics Magnification Biodentine Mineral trioxide aggregate Apexification Open apex

dental pulp stem cells and banking of teeth as a lifesaving therapeutic vista

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BIOCELL 2023年第1期47卷 71-80页

作者： SUKUMARAN ANIL RAMYA RAMADOSS NEBU GTHOMAS JASMIN MGEORGE VISHNUPRIYA KSWEETY Oral Health Institute Department of DentistryHamad Medical CorporationDohaQatar College of dental Medicine Qatar UniversityDohaQatar Saveetha dental College Saveetha Institute of Medical and Technical Sciences(SIMATS)ChennaiIndia Pushpagiri College of dental Sciences MedicityPerumthuruthyIndia

Exfoliated deciduous or an extracted healthy adult tooth can be used to harvest,process,and cryogenically preserve dental pulp stem *** stem cell-based regenerative medicine methods could benefit significantly from these mesenchymal stem *** serve as a substantial source of mesenchymal stem cells,otherwise disposed of as medical *** should be taken to store this treasure trove of stem *** responsibility of patients,dentists,and physicians is necessary to ensure that this valuable resource is not wasted and that every possible dental pulp stem cell is available for use in the *** dental pulp stem cells(DPSC)inside teeth represent a significant future source of stem cells for regenerative medicine *** review describes the ontogeny,the laboratory processing and collection,and isolation methods of *** review also discusses currently available stem cell banking facilities and their potential use in regenerative medicine procedures in dental and general medical applications in the future.

关键词： dental stem cell Stem cell therapy Differentiation Regeneration Tissue engineering Tooth banking

Identification of shared and unique gene families associated with oral clefts

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International Journal of Oral Science 2017年第2期9卷 104-109页

作者： Noriko Funato Masataka Nakamura Department of Signal Gene Regulation Research Center for Medical and Dental SciencesTokyo Medical and Dental University1-5-45 YushimaBunkyo-kuTokyoJapan

Oral clefts, the most frequent congenital birth defects in humans, are multifactorial disorders caused by genetic and environmental factors. Epidemiological studies point to different etiologies underlying the oral cleft phenotypes, cleft lip(CL),CL and/or palate(CL/P) and cleft palate(CP). More than 350 genes have syndromic and/or nonsyndromic oral cleft associations in humans. Although genes related to genetic disorders associated with oral cleft phenotypes are known, a gap between detecting these associations and interpretation of their biological importance has remained. Here, using a gene ontology analysis approach, we grouped these candidate genes on the basis of different functional categories to gain insight into the genetic etiology of oral clefts. We identified different genetic profiles and found correlations between the functions of gene products and oral cleft phenotypes. Our results indicate inherent differences in the genetic etiologies that underlie oral cleft phenotypes and support epidemiological evidence that genes associated with CL/P are both developmentally and genetically different from CP only, incomplete CP, and submucous CP. The epidemiological differences among cleft phenotypes may reflect differences in the underlying genetic causes. Understanding the different causative etiologies of oral clefts is important as it may lead to improvements in diagnosis, counseling, and prevention.

关键词： cleft palate epidemiology gene ontology mutations soft palate syndrome

Transfection of bone marrow mesenchymal stem cells using green fluorescence protein labeled hVEGF165 recombinant plasmid mediated by liposome

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Asian Pacific Journal of Tropical Medicine 2013年第9期6卷 739-742页

作者： Tao Wang Tian-An Liao Shao-Bo Zhong dental medical center People's Hospital of Hainan Province

Objective:To study the role of bone marrow mesenchymal stem cells(BMSCs)in construction of vascularized engineered ***:hVEGF165 was amplified via RT-PCR before recombinant with pShuttle-green fluorescence protein;green fluorescent protein(GFP)-*** the recombinant shuttle plasmid was transfected into BMSCs with Lipofectamine^(TM)2000 for packaging and ***165 mRNA expression in BMSCs cells was ***:The sequence of hVEGFI65 in pShutlle-GFP-hVFGF165 plasmid was confirimed by double-enzyme cleavage method and ***165 was highly expressed in ***:The GFP/hVECF165 recombinant plasmid vector was constructed successfully and expressed effectively in host cells,which may be helpful for discussing the possibility of the application of VEGF165-BMSCs in tissue engineering and ischemic disease cure.

关键词： Vascular endothelial growth factor Green fluorescent protein Bone marrow mesenchymal stem cells Plasmid

The synergistic effect of honey and cinnamon against Streptococcus mutans bacteria

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Asian Pacific Journal of Tropical Biomedicine 2017年第4期7卷 314-320页

作者： Mohammad Bagher Rezvani Mohammad Niakan Mohammad Kamalinejad Fateme Sadat Ahmadi Faeze Hamze Operative Department Shahed Dental SchoolShahed University Microbiology Department Shahed Medical SchoolShahed University School of Pharmacy Shahid Beheshti University of Medical Sciences dental Research center Shahed Dental SchoolShahed University Oral and dental Disease Research center Kerman University of Medical Science

Objective: To investigate the effect of Iranian honey, cinnamon and their combination against Streptococcus mutans ***: Nine experimental solutions were examined in this study, including two types of honey(pasteurized and sterilized), two types of cinnamon extract(dissolved in distilled water or dimethyl sulfoxide) and five different mixtures of cinnamon in honey(prepared by admixing 1%–5% w/w of cinnamon extract into 99%–95% w/w of honey, respectively).Meanwhile, each of mentioned agent was considered as the first solution while it was diluted into seven serially two-fold dilutions(from 1:2 to 1:128 v/v).Therefore, eight different concentrations of each agent were *** antibacterial tests were performed through blood agar well diffusion method, and the minimum inhibitory concentration(MIC) was ***, the data were subjected to statistical analysis incorporating Two-way ANOVA and Bonferroni post hoc tests(a = 0.01).Results: The highest zone of inhibition was recorded for the mixtures of honey and cinnamon while all the subgroups containing 95%–99% v/v of honey were in the same range(P < 0.01).The MIC for both honey solutions were obtained as 500 mg/mL whereas it was 50 mg/m L for both cinnamon ***, the MIC related to all honey/cinnamon mixtures were 200 mg/***: A profound synergistic effect of honey and cinnamon was observed against Streptococcus mutans while there was no significant difference among extracts containing 99%–95% v/v of honey admixing with 1%–5% v/v of cinnamon, respectively.

关键词： Synergistic effect Honey Cinnamon Streptococcus mutans