检索结果-南通市图书馆

An optimized derivative of an endogenous CXCr4 antagonist prevents atopic dermatitis and airway inflammation

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2021年第9期11卷 2694-2708页

作者： Mirja Harms Monica MWHabib Simona Nemska Antonella Nicolò Andrea Gilg Nico Preising Pandian Sokkar Sara Carmignani Martina raasholm Gilbert Weidinger Gönül Kizilsavas Manfred Wagner Ludger Ständker Ashraf HAbadi Hassan Jumaa frank Kirchhoff Nelly frossard Elsa Sanchez-Garcia Jan Münch institute of Molecular Virology Ulm University Medical CenterUlm 89081Germany Department of Pharmaceutical Chemistry Faculty of Pharmacy and BiotechnologyGerman University in CairoCairo 11835Egypt Laboratoire D'Innovation Thérapeutique UMR7200 CNRS/Universitéde Strasbourg and LabEx MEDALISFacultéde PharmacieIllkirch 67412France institute of Immunology Ulm University Medical CenterUlm 89081Germany Core facility functional Peptidomics Ulm University Medical CenterUlm 89081Germany Center of Medical Biotechnology University of Duisburg-EssenDuisburg 47057Germany max-planck-institute for Coal research Düsseldorf 45470Germany faculty of Allied Health Sciences Chettinad Hospital and Research InstituteChettinad Academy of Research and EducationKelambakkam 603103India max-planck-institut für molekulare physiologie Dortmund 44227Germany institute of Biochemistry and Molecular Biology Ulm UniversityUlm 89081Germany max planck institute for Polymer research Mainz 55128Germany

Aberrant CXCr4/CXCL12 signaling is involved in many pathophysiological processes such as cancer and inflammatory diseases.A natural fragment of serum albumin,named EPI-X4,has previously been identified as endogenous peptide antagonist and inverse agonist of CXCr4 and is a promising compound for the development of improved analogues for the therapy of CXCr4-associated *** generate optimized EPI-X4 derivatives we here performed molecular docking analysis to identify key interaction motifs of EPI-X4/*** rational drug design allowed to increase the anti-CXCr4 activity of *** EPI-X4 derivative JM#21 bound CXCr4 and suppressed CXCr4-tropic HIV-1 infection more efficiently than the clinically approved small molecule CXCr4 antagonist ***-X4 JM#21 did not exert toxic effects in zebrafish embryos and suppressed allergen-induced infiltration of eosinophils and other immune cells into the airways of animals in an asthma mouse ***,topical administration of the optimized EPI-X4 derivative efficiently prevented inflammation of the skin in a mouse model of atopic ***,rationally designed EPIX4 JM#21 is a novel potent antagonist of CXCr4 and the first CXCr4 inhibitor with therapeutic efficacy in atopic *** clinical development of this new class of CXCr4 antagonists for the therapy of atopic dermatitis,asthma and other CXCr4-associated diseases is highly warranted.

关键词： CXCr4 antagonist Asthma Allergy Inflammation Atopic dermatitis

Merging radical catalysis and noncovalent stereocontrol concepts toforge native O-glycosidic linkages

在线全文

学校读者我要写书评

暂无评论

Science Bulletin 2024年第21期69卷 3308-3310页

作者： Hao Guo Charles C.J.Loh Abteilung Chemische Biologie Max Planck Institut für Molekulare PhysiologieOtto-Hahn-Straße 1144227 DortmundGermany fakültät für Chemie und Chemische Biologie Technische Universität DortmundOtto-Hahn-Straße 4a44227 DortmundGermany UCD School of Chemistry University College DublinBelﬁeld Dublin 4Ireland

Stereoselective carbohydrate synthesis[1,2]has experiencedimmense strides in the last few years,beneﬁting from assimilatingknowledge at the frontiers of modern synthetic *** thisfront,the dual but parallel development of radical catalysis in glycosylations[3–6]and the tapping of weak noncovalent interactions(NCIs)[7–11]for catalytic stereocontrol in stereoselective carbohydrate synthesis are of immense *** is important to emphasize that these two broad pillars were previously relativelyunheard of in theﬁeld of classical carbohydrate chemistryﬁveyears ago,but since then they have substantially established themselves in the mild and efﬁcient construction of glycosidic linkages[12].The precise catalytic control of the stereochemistry of nativeglycosidic linkages had historically been challenging,as this seemingly simple C–O bond forming step required the concurrent tackling of two fundamental selectivity challenges(fig.1a):Namelythe anomeric selectivity challenge to construct the C1 acetal andthe site-selectivity challenge[13,14]to discriminate the numeroushydroxyl groups on a carbohydrate polyol substrate.

关键词： catalysis selectivity radical

Clinically detected gastroenteropancreatic neuroendocrine tumors are on the rise: Epidemiological changes in Germany

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2013年第47期19卷 9012-9019页

作者： Hans Scherübl Brigitte Streller roland Stabenow Hermann Herbst Michael Hopfner Christoph Schwertner Joachim Steinberg Jan Eick Wanda ring Krishna Tiwari Soren M Zappe Klinik für Gastroenterologie GI Onkologie und InfektiologieVivantes Klinikum Am Urban10967 BerlinGermany Gemeinsames Krebsregister der Lander Berlin BrandenburgMecklenburg-VorpommernSachsen-Anhalt und der Freistaaten Sachsen und Thüringen (GKR)10967 BerlinGermany institut für Pathologie Vivantes Netzwerk für Gesundheit GmbH10967 BerlinGermany institut für physiologie Charité-Universitatsmedizin10117 BerlinGermany Charité-Universitatsmedizin Berlin HNO-KlinikCampus Virchow Klinikum13353 BerlinGermany

AIM:To study the epidemiologic changes of gastroenteropancreatic neuroendocrine tumors(GEP-NET)in Germany,we analyzed two time periods 1976-1988 ***:We evaluated epidemiological data of GEP-NET from the former East German National Cancer registry(DDr Krebsregister,1976-1988)and its successor,the Joint Cancer registry(GKr,1998-2006),which was founded after German *** to a particularly substantial database the epidemiological data from the federal states of Mecklenburg-Western Pomerania,Saxony,Brandenburg and Thuringia,covering a population of more than 10.8 million people,were *** probabilities were calculated using life table *** addition,GEP-NET patients were evaluated for one or more second(non-GEP-NET)primary ***:A total of 2821 GEP neuroendocrine neoplasms were identified in the two *** overall incidence increased significantly between 1976 and2006 from 0.31(per 100.000 inhabitants per year)to2.27 for men and from 0.57 to 2.38 for *** the later period studied(2004-2006),the small intestine was the most common ***(NE)neoplasms of the small intestine showed the largest absolute increase in incidence,while rectal NE neoplasms exhibited the greatest relative *** the incidence of appendiceal NET in women showed little change between 1976 and *** survival of patients varied for sex,tumor site and the two periods studied but improved significantly over ***,about 20%of the GEP-NET patients developed one or more second *** most common location was the gastrointestinal ***-NET patients without second malignancies fared better than those with one or more of ***:The number of detected GEP-NET increased about 5-fold in Germany between 1976 *** the same time,their anatomic distribution changed,and the survival of GEP-NET patients improved *** malignancies are common and influence the ov

关键词： Neuroendocrine Tumor Epidemiology Gastrinoma Insulinoma Endoscopy German history reunification Second malignancy

Epoxyeicosatrienoic acids:impact on signal transduction,gene expression and function of vascular cells

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Epoxyeicosatrienoic acids:impact on signal transduction,gene...

Interleukin-12 as a Genetic Adjuvant Enhances Hepatitis C Virus NS3 DNA Vaccine Immunogenicity

11th International Vascular Neuroeffector Mechanisms and Cardiovascular Pharmacology and Medicine Symposia, Shanghai-Suzhou 2006

作者： rudi Busse institut für Kardiovaskulre physiologie Johann Wolfgang Goethe-Universitt frankfurt Germany

Nitric oxide and prostacyclin-independent vasodilatation in several vascular beds has been linked to the activation of cytochrome P450 (CYP) epoxygenases expressed in endothelial cells and the generation of vasodilator epoxyeicosatrienoic acids (EETs). EETs contribute to the activation of small and intermediate Ca2+-dependent K+ channels in endothelial cells and the subsequent hyperpolarization, by a mechanism that involves the opening of TrP channels and

关键词： CYP Epoxyeicosatrienoic acids:impact on signal transduction,gene expression and function of vascular cells

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Virologica Sinica 2013年第3期28卷 167-173页

作者： Malihe Naderi Atefeh Saeedi Abdolvahab Moradi Mishar Kleshadi Mohammad reza Zolfaghari Ali Gorji Amir Ghaemi Department of Microbiology Qom branch Islamic Azad University Department of Microbiology Lahijan Branch Islamic Azad University Golestan research Center of Gastroenterology and Hepatology-GrCGH Department of Microbiology Golestan University of Medical Sciences Shefa Neuroscience research Centre institut für physiologie I Westflische Wilhelms-Universitt Münster Klinik und Poliklinik für Neurochirurgie Westf lische Wilhelms-Universitt Münster

Hepatitis C virus (HCV) chronic infection is a worldwide health problem, and numerous efforts have been invested to develop novel vaccines. An efficient vaccine requires broad immune response induction against viral proteins. To achieve this goal, we constructed a DNA vaccine expressing nonstructural 3 (NS3) gene (pcDNA3.1-HCV-NS3) and assessed the immune response in C57BL/6 mice. In this study, the NS3 gene was amplified with a nested-reverse transcriptase-polymerase chain reaction (rT-PCr) method using sera of HCV-infected patients with genotype 1a. The resulting NS3 gene was subcloned into a pcDNA3.1 eukaryotic expression vector, and gene expression was detected by western blot. The resultant DNA vaccine was co-administered with interleukin-12 (IL-12) as an adjuvant to female C57BL/6 mice. After the final immunizations, lymphocyte proliferation, cytotoxicity, and cytokine levels were assessed to measure immune responses. Our data suggest that co-administration of HCV NS3 DNA vaccine with IL-12 induces production of significant levels of both IL-4 and interferon (IfN)-γ (proliferation responses of vaccinated mice were significantly increased compared to control (pr results demonstrated that co-administration of HCV NS3 and IL-12 displayed strong immunogenicity in a murine model.

关键词： Hepatitis C virus (HCV) NS3 Interleukin-12 DNA vaccine

The GYf domain protein CD2BP2 is critical for embryogenesis and podocyte function

在线全文

学校读者我要写书评

暂无评论

Journal of Molecular Cell Biology 2015年第5期7卷 402-414页

作者： Gesa I.Albert Christoph Schell Karin M.Kirschner Sebastian Schafer ronald Naumann Alexandra Muller Oliver Kretz Benno Kuropka Mathias Girbig Norbert Hubner Eberhard Krause Holger Scholz Tobias B.Huber Klaus-Peter Knobeloch Christian freund institute for Chemistry and Biochemistry Freie Universitat Berlin14195 BerlinGermany Leibniz-institut fuer molekulare Pharmakologie 13125 BerlinGermany renal Division University Hospital Freiburg79106 FreiburgGermany Speman Graduate School for Medicine and Biology University of Freiburg79106 FreiburgGermany faculty of Biology University Freiburg79106 FreiburgGermany institute fuer Vegetative physiologie Charité-Universitatsmedizin Berlin10117 BerlinGermany Experimental Genetics and Cardiovascular Diseases MDC13125 BerlinGermany Transgenic Core facility Max-Planck-Institute of Molecular Cell Biology and GeneticsDresdenGermany institute of Neuropathology University of Freiburg79106 FreiburgGermany institute of Cell Biology&Anatomy University of Freiburg79106 FreiburgGermany BIOSS Centre for Biological Signaling Studies Albert-Ludwigs-University79106 FreiburgGermany

Scaffolding proteins play pivotal roles in the assembly of macromolecular machines such as the *** adaptor protein CD2BP2,originally identified as a binding partner of the adhesion molecule CD2,is a pre-spliceosomal assembly factor that utilizes its glycine-tyrosine-phenylalanine(GYf)domain to co-localize with spliceosomal *** far,its function in vertebrates is *** conditional gene targeting in mice,we show that CD2BP2 is crucial for embryogenesis,leading to growth retardation,defects in vascularization,and premature death at embryonic day 10.5 when *** of the protein in bone marrow-derived macrophages indicates that CD2BP2 is involved in the alternative splicing of mrNA transcripts from diverse *** the molecular level,we identified the phosphatase PP1 to be recruited to the spliceosome via the N-terminus of *** the strong expression of CD2BP2 in podocytes of the kidney,we use selective depletion of CD2BP2,in combination with next-generation sequencing,to monitor changes in exon usage of genes critical for podocyte functions,including VEGf and actin ***2BP2-depleted podocytes display foot process effacement,and cause proteinuria and ultimately lethal kidney failure in ***,our study defines CD2BP2 as a non-redundant splicing factor essential for embryonic development and podocyte integrity.

关键词： GYf domain CD2BP2 alternative splicing PP1 podocytes VEGf

碘化N-正丁基氟哌啶醇对豚鼠心房肌细胞乙酰胆碱敏感性钾通道的影响

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国药理学通报 2005年第5期21卷 544-550页

作者：刘冰石刚刚高分飞 Lutz Pott 汕头大学医学院药物研究室 institut fr physiologie ruhr-Universitt Bochum D44780 BochumGermany

目的研究碘化N正丁基氟哌啶醇(f2)对豚鼠心房肌细胞乙酰胆碱敏感性钾通道(KACh)的影响,探讨其对KACh的作用机制。方法采用膜片钳全细胞记录方法,测定f2对原代培养的豚鼠心房肌细胞乙酰胆碱敏感性钾电流IK(ACh)的影响。结果细胞外给予f2... 详细信息

目的研究碘化N正丁基氟哌啶醇(f2)对豚鼠心房肌细胞乙酰胆碱敏感性钾通道(KACh)的影响,探讨其对KACh的作用机制。方法采用膜片钳全细胞记录方法,测定f2对原代培养的豚鼠心房肌细胞乙酰胆碱敏感性钾电流IK(ACh)的影响。结果细胞外给予f2对豚鼠心房肌细胞IK(ACh)呈可逆性、浓度依赖性的阻断作用。细胞内添入抗水解的GTP类似物GTPγS后,结果同前。细胞内给予50μmol·L-1f2对IK(ACh)无作用。结论f2是豚鼠心房肌细胞KACh的一种快速通道阻断剂,发挥作用部位在细胞膜外侧,作用位点在钾通道本身,与乙酰胆碱受体无关。

关键词：碘化N-正丁基氟哌啶醇心房肌细胞乙酰胆碱敏感性钾通道(KACh) 膜片钳全细胞记录 G蛋白激活钾通道

Effects of Cadmium on Polar Lipid Composition and Unsaturation in Maize(Zea mays) in Hydroponic Culture

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2007年第12期49卷 1693-1702页

作者： radhouane Chaffai Tinni Nouhou Seybou Brahim Marzouk Ezzedine EI ferjani Laboratoire de Bio-physiologie Cellulaires Vegetales Faculte des Sciences de Bizerte 7021 Zarzouna-BizerteTunisie Laboratoire ErTAC physiologie et Genetique Vegetales 63177 AubiereClermont FerrandFrance Laboratoire d'Adaptation et d'Amelioration des Plantes Institut National de la Recherche Scientifique et Technique(INRST)BP 95-2050 Hammam-LifTunisie

This study aimed to evaluate the effect of Cd exposure （100 μmol/L） on polar lipid composition, and to examine the level of fatty acid unsaturation in maize （Zea mays L.）. In roots, the level of 16：0 and monounsaturated fatty acids （16：1 ＋ 18：1） decreased in phosphatidylcholine （PC） and phosphatidylethanolamine （PE）. In contrast, the proportion of unsaturated 18-C fatty acid species showed an opposite response to Cd. The content, on the other hand, of PC, PE, digalactosyldiacylglycerol （DGDG）, and steryl lipids increased in roots （2.9-, 1.6-, 5.3-, and 1.7-fold increase, respectively）. These results suggest that a more unsaturated fatty acid composition than found in control plants with a concomitant increase in polar lipids may favor seedling growth during Cd exposure. However, the observed increase in the steryl lipid （SL）： phospholipid （PL） ratio （twofold）, the decrease in monogalactosyldiacylglycerol （MGDG）： DGDG ratio, as well as the induction of lipid peroxidation in roots may represent symptoms of membrane injury. In shoots, the unsaturation level was markedly decreased in PC and phosphatidylglycerol （PG） after Cd exposure, but showed a significant increase in sulfoquinovosyldiacylglycerol （SQDG）, MGDG and DGDG. The content of PG and MGDG was decreased by about 65%, while PC accumulated to higher levels （4.4-fold increase）. Taken together, these changes in the polar lipid unsaturation and composition are likely to be due to alterations in the glycerolipid pathway. These results also support the idea that the increase in overall unsaturation plays some role in enabling the plant to withstand the metal exposure.

关键词： cadmium fatty acids heavy metal stress lipid peroxidation polar lipids unsaturation level Zea mays.

The Influence of Apple-or red-Grape Pomace Enriched Piglet Diet on Blood Parameters,Bacterial Colonisation,and Marker Gene Expression in Piglet White Blood Cells

在线全文

学校读者我要写书评

暂无评论

food and Nutrition Sciences 2011年第4期2卷 366-376页

作者： Julia Sehm Dieter Treutter Hermann Lindermayer Heinrich H.D.Meyer Michael W.Pfaffl Lehrstuhl für physiologie Weihenstephan Zentralinstitut für Ernahrung-und LebensmittelforschungWissenschaftszentrum WeihenstephanTechnische Universitat MünchenFreisingGermany fachgebiet Obstbau Weihenstephan Wissenschaftszentrum WeihenstephanTechnische Universitat MünchenFreisingGermany institut für Tierernahrung und futterwirtschaft Bayerische Landesanstalt für LandwirtschaftPoing-GrubGermany

Proanthocyanidins and flavanoids, both subfamilies of the polyphenols, are highly concentrated in different fruits and berries as well as in fruit pomace. They have shown to exhibit anti-cancer, anti-microbial, anti-oxidative, and im- mune-modulatory effects in vertebrates. Herein the effect of additional apple pomace or red-grape pomace in conven- tional piglet starter feeds were investigated in 36 young growing piglets. Immunological marker gene expression was quantified by quantitative real-time rT-PCr in white blood cells, and intestinal bacterial flora was investigated from weaning to three weeks post weaning. Polyphenol content in red-grape pomace, gut content and tissues were analyzed with HPLC. flavan-3-ols (epicatechin and catechin) and proanthocyanidins (B1, B2 and C1) were identified in the gas- tro-intestinal tract content, whereas only traces could be detected in various piglet organs. The blood parameters, he- moglobin and hematocrit, were affected and down-regulated in all groups over testing period. In both pomace treated groups more thrombocytes were present compared to the standard feeding group. It turns out, that the pomace diets had greatest impact on the bacterial content in the colon. results demonstrate that feeding apple pomace and red- grape pomace tended to increase the number of total colonic bacteria. Steptococci/Enterococci increased in the red- grape pomace. C. perfringens was not detectable at the second time point. The number of lactobacilli increased in both applied diets. The number of Clostridium perfringens decreased with the age of the piglets. Trends of mrNA expression changes were found in white blood cell (WBC) between different feeding regimens, since the expression variability in the groups was very high. Between the different time points there were significant differences within the apple pomace group, where TNf? (p = 0.033), Nf?B (p = 0.024) and Caspase 3 (p = 0.019) mrNA expression increased signifi- cantly during treatm

关键词： Apple Pomace red-Grape Pomace White Blood Cells mrNA Gene Expression Blood Parameters Bacterial Colonization