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A highly selective and instantaneous upconversion fluorescent nanoprobe for ascorbic acid detection in biological samples

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Chinese Chemical Letters 2017年第10期28卷 1983-1986页

作者： Yuanyuan Chen Tingting Zhang Xiaonan Gao Wei Pan Na Li Bo Tang College of Chemistry Chemical Engineering and Materials Science Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong Key Laboratory of Molecular and Nano Probes Ministry of Education Institute of Moleeular and Nano Science Shandong Normal University Ji'nan 250014 China

Ascorbic acid（AA） serves as a key coenzyme in many metabolic pathways. Enough daily AA supplements from different dietary sources are the only way for human to maintain their AA levels in *** of AA content in different foods guides to build healthy diet, which is of great biomedical significance. Hence, developing a highly selective and instantaneous fluorescent nanoprobe for the detection of AA in biological samples is highly needed. Here we present a novel turn-on fluorescent nanoprobe using lanthanide-doped upconversion nanoparticles（UCNPs） and cobalt oxyhydroxide（Co OOH） nanoflakes for monitoring AA in fruit samples. In this nanosystem, the UCNPs can be adsorbed onto the Co OOH nanoflakes, leading to a remarkable fluorescence decrease through Fo？rster resonance energy transfer. Furthermore, the AA could trigger the disassembly of the Co OOH to liberate the upconverted fluorescence. The UCNPs-based nanoprobe can provide an effective platform for highly selective and rapid detection of AA in biological samples.

关键词： Cobalt oxyhydroxide nanoflakes Upconversion nanoparticles Ascorbic acid Fluorescent nanoprobe Biological samples

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Activation of the Astrocytic Endothelin System in Response to Hypoxia

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中国临床神经科学 2000年第Z1期8卷 97-页

作者： An Ding Xua Kai M.Schmidt-Ott Scbastian Tuschick Lutz Liefeldt Susan Lyons Helmut Kettcnmann Martin.Paul Institute of Clinical Pharmacology and Toxicology Benjamin Franklin Medical CenterBerlinGermany Dept.Neurology Jinan University GuangzhouP.R.of China Institute of Clinical Pharmacology and Toxicology Benjamin Franklin Medical CenterBerlinGermany max-delbrück center for moleeular medicine Berlin-BuchGermany

Objcctive:To determine the gene expression patterns of endothelin (ET)system components in cultured astrocytes(AC),and to examine the direct effcct of hypoxia on ET system gene expression in cultured ***:The ET system was considered to be related to the activation of AC. However,how hypoxia affects the ET system in transcriptional levels remains ***:AC was prepared form mouse brain,and cultured 4 *** further incubated under normoxic or hypoxic conditions for 24h. ET peptide levels were determined by *** transcripts of ET system components were measured by Northern Blot RNA hybridization and ***:In normoxic AC,ET-1,ET converting enzyme(ECE)-2,ETA receptor,and ETB receptor mRNAs were detected by Northern blot hybridization with ETB receptor mRNA appearing to be the predominant receptor ***-3and ECE-1 were only detected by RT-PCR,indicating low expression levels of these *** induced a 1.7-fold increase in ET peptide level in culture supernatants as comparcd to controls(p＜0.001).At the same time,a 3-fold increase of ET-1 mRNA(p＜0.001)was determined by Northern blot RNA analysis,indicating a regulation at the transcriptional *** ETA and ETB receptor mRNASweredownregulated to approximately 20% of control levels(p＜0.001), while ECE-2 mRNA remained ***:These results indicate direct effects of hypoxia on astrocytic ET system gene ***,similar changes observed in ischemic conditions in vivo are likely to be at least partially independent from the modified cerebral microenvironment.

关键词： gene expression Northern Blot Northern blot hybridization

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Separation and Identification of Flavonoids from Fistular Onion Stalk(Allium fisturosum *** Makio)

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2010年第2期30卷 255-257页

作者：付琴琴刘静幽张长弓周银波张耕马丹杨新洲 Department of Pharmacognosy Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China center of Preparations the First Hospital of Wuhan City Wuhan 430022 China The Eskitis Institute for Cell and moleeular Therapies Griffith University Brishbane 4111 Australia

The chemical constituents of fistular onion stalk obtained by supercritical CO2 extraction were separated and purified by silica gel and sephadex LH-20 gel column chromatography and the preparative TLC method and four flavonoids were obtained. On the basis of the spectral data, they were structurally identified as （＋）-catechin, （-）-epieatechin, astragalin, and 3-O-β-D（2-O-β-D-glu- copyranosyl）-glucopyranosides of kaempferol.

关键词： fistular onion stalk allium fisturosum supercritical CO2 extraction chemical constituents flavonoids

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