检索结果-南通市图书馆

Synthetic biology identifies the minimal gene set required for paclitaxel biosynthesis in a plant chassis

molecular plant 2023年第12期16卷 1951-1961页

作者： Youjun Zhang Lorenz Wiese Hao Fang Saleh Alseekh Leonardo Perez de Souza Federico Scossa John Molloy Mathias Christmann Alisdair R.Fernie max-planck-Institut fur Molekulare Pflanzenphysiologie Am Muhlenberg 114476 Potsdam-GolmGermany Center of plant Systems Biology and Biotechnology 4000 PlovdivBulgaria institute of Chemistry and Biochemistry Freie Universitat BerlinTakustraBe 314195 BerlinGermany Department of Biomolecular Systems Max Planck Institute of Colloids and Interfaces14476 PotsdamGermany Research Center for Genomics and Bioinformatics(CREA-GB) Via Ardeatina 54600178 RomeItaly

The diterpenoid paclitaxel(Taxol)is a chemotherapy medication widely used as a first-line treatment against several types of solid cancers.The supply of paclitaxel from natural sources is limited.However,missing knowledge about the genes involved in several specific metabolic steps of paclitaxel biosynthesis has rendered it difficult to engineer the full pathway.In this study,we used a combination of transcriptomics,cell biology,metabolomics,and pathway reconstitution to identify the complete gene set required for the heterologous production of paclitaxel.We identified the missing steps from the current model of paclitaxel biosynthesis and confirmed the activity of most of the missing enzymes via heterologous expression in Nicotiana benthamiana.Notably,we identified a new C4β-C20 epoxidase that could overcome the first bottleneck of metabolic engineering.We used both previously characterized and newly identified oxomutases/epoxidases,taxane 1β-hydroxylase,taxane 9aα-hydroxylase,taxane 9α-dioxygenase,and phenylalanine-CoA ligase,to successfully biosynthesize the key intermediate baccatin Ill and to convert baccatin Ill into paclitaxel in N.benthamiana.In combination,these approaches establisha metabolic route to taxoidbiosynthesis and provide insights into the unique chemistry that plants use to generate complex bioactive metabolites.

关键词： paclitaxel biosynthesis synthetic biology baccatin Ill biosynthesis Taxus

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Targeted approaches to improve tomato fruit taste

引用

Horticulture Research 2023年第1期10卷 57-68页

作者： Shouchuang Wang Qi Qiang Lijun Xiang Alisdair R.Fernie Jun Yang Sanya Nanfan Research institute of Hainan University Hainan Yazhou Bay Seed LaboratorySanya572025China College of Tropical Crops Hainan UniversityHaikou570228China max planck institute of molecular plant physiology Potsdam-Golm 14476Germany

Tomato(Solanum lycopersicum)is the most valuable fruit and horticultural crop species worldwide.Compared with the fruits of their progenitors,those of modern tomato cultivars are,however,often described as having unsatisfactory taste or lacking f lavor.The f lavor of a tomato fruit arises from a complex mix of tastes and volatile metabolites,including sugars,acids,amino acids,and various volatiles.However,considerable differences in fruit f lavor occur among tomato varieties,resulting in mixed consumer experiences.While tomato breeding has traditionally been driven by the desire for continual increases in yield and the introduction of traits that provide a long shelf-life,consumers are prepared to pay a reasonable premium for taste.Therefore,it is necessary to characterize preferences of tomato f lavor and to define its underlying genetic basis.Here,we review recent conceptual and technological advances that have rendered this more feasible,including multi-omics-based QTL and association analyses,along with the use of trained testing panels,and machine learning approaches.This review proposes how the comprehensive datasets compiled to date could allow a precise rational design of tomato germplasm resources with improved organoleptic quality for the future.

关键词： breeding cultivar render

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Balancing nutrients in a toxic environment: the challenge of eating

引用

Insect Science 2022年第1期29卷 289-303页

作者： Peng Wang Daniel G.Vassão Bettina Raguschke Michael J.Furlong Myron P.Zalucki School of Biological Sciences The University of QueenslandSt LuciaQueenslandAustralia Department of Biochemistry Max Planck Institute for Chemical EcologyJenaGermany

Insect herbivores can regulate their food intake by mixing food sources with different nutrient content, but face the resulting challenge of ingesting various plant secondary metabolites. How insects deal with toxins in a complex nutrient environment is unclear. Here we investigated the influence of a classic plant secondary metabolite, allyl glucosinolate (sinigrin), and its hydrolyzed product allyl isothiocyanate (AITC), on the development of Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) when fed on diets with different protein-to-carbohydrate (p : c) ratios. We also examined the effects of these toxins on larval biochemistry, by chemically analyzing the frass produced by insects feeding on the different diets. As expected, AITC had a greater negative effect than sinigrin on H. armigera life-history traits. However, AITC at low concentration appeared to have a positive effect on some traits. Both sinigrin and AITC-induced detoxification activity in the gut, and the reaction was related to diet protein concentration. High-protein diets can provide the required free amino acid, especially cysteine, needed for the detoxification process. The nutrient content of the diet influences how plant secondary metabolites are handled, and the use of artificial diets in experiments investigating the metabolic fate of plant secondary compounds needs to be carefully evaluated.

关键词： diet protein glucosinolate gut metabolism Helicoverpa armigera isothiocyanate

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Growth-Regulating Factors （GRFs）： A Small Transcription Factor Family with Important Functions in plant Biology

引用

molecular plant 2015年第7期8卷 998-1010页

作者： Mohammad Amin Omidbakhshfard Sebastian Proost Ushio Fujikura Bernd Mueller-Roeber institute of Biochemistry and Biology University of Potsdam Karl-Liebknecht-Straβe 24-25 Haus 20 14476 Potsdam-Golm Germany max-planck institute of molecular plant physiology Am Muhlenberg 1 14476 Potsdam-Golm Germany

Growth-regulating factors （GRFs） are plant-specific transcription factors that were originally identified for their roles in stem and leaf development, but recent studies highlight them to be similarly important for other central developmental processes including flower and seed formation, root development, and the coordination of growth processes under adverse environmental conditions. The expression of several GRFs is controlled by microRNA miR396, and the GRF-miRNA396 regulatory module appears to be central to several of these processes. In addition, transcription factors upstream of GRFs and miR396 have been discovered, and gradually downstream target genes of GRFs are being unraveled. Here, we review the current knowledge of the biological functions performed by GRFs and survey available molecular data to illustrate how they exert their roles at the cellular level.

关键词： abiotic stress chromatin remodeling flower development growth regulation leaf development,miRNA

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Transcription factor CsESE3 positively modulates both jasmonic acid and wax biosynthesis in citrus

引用

aBIOTECH 2022年第4期3卷 250-266页

作者： Haoliang Wan Haiji Qiu Zhuoran Li Xiaoliang Zhang Jingyu Zhang Deyuan Jiang Alisdair RFernie Yi Lyu Yunjiang Cheng Weiwei Wen Key Laboratory of Horticultural plant Biology(MOE) College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhan430070China Shenzhen institute of Nutrition and Health Huazhong Agricultural UniversityWuhan430070China Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen518000China max-planck-institute of molecular plant physiology Am Muehlenberg 114476PotsdamGermany Key Laboratory for Space Bioscience and Biotechnology School of Life SciencesNorthwestern Polytechnical UniversityXi’an710072China

PLIP lipases can initiate jasmonic acid(JA)biosynthesis.However,little is known about the transcriptional regulation of this process.In this study,an ERF transcription factor(CsESE3)was found to be co-expressed with all necessary genes for JA biosynthesis and several key genes for wax biosynthesis in transcriptomes of‘Newhall’navel orange.CsESE3 shows partial sequence similarity to the well-known wax regulator SHINEs(SHNs),but lacks a complete MM protein domain.Ectopic overexpression of CsESE3 in tomato(OE)resulted in reduction of fruit surface brightness and dwarf phenotype compared to the wild type.The OE tomato lines also showed significant increases in the content of wax and JA and the expression of key genes related to their biosynthesis.Overexpression of CsESE3 in citrus callus and fruit enhanced the JA content and the expression of JA biosynthetic genes.Furthermore,CsESE3 could bind to and activate the promoters of two phospholipases from the PLIP gene family to initiate JA biosynthesis.Overall,this study indicated that CsESE3 could mediate JA biosynthesis by activating PLIP genes and positively modulate wax biosynthesis.The findings provide important insights into the coordinated control of two defense strategies of plants represented by wax and JA biosynthesis.

关键词： Citrus Cuticular wax Jasmonic acid SHINE transcription factor Lipase

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Metabolomics-assisted refinement of the pathways of steroidal glycoalkaloid biosynthesis in the tomato clade

引用

Journal of Integrative plant Biology 2014年第9期56卷 864-875页

作者： Kevin Schwahn Leonardo Perez de Souza Alisdair R.Fernie Takayuki Tohge University of Potsdam AG BioinformaticsInstitute for Biochemistry and Biology maxplanck-institute of molecular plant physiology

Steroidal glycoalkaloids（SGAs） are nitrogen-containing secondary metabolites of the Solanum species,which are known to have large chemical and bioactive diversity in nature.While recent effort and development on LC/MS techniques for SGA profiling have elucidated the main pathways of SGA metabolism in tomato,the problem of peak annotation still remains due to the vast diversity of chemical structure and similar on overlapping of chemical formula.Here we provide a case study of peak classification and annotation approach by integration of species and tissue specificities of SGA accumulation for provision of comprehensive pathways of SGA biosynthesis.In order to elucidate natural diversity of SGA biosynthesis,a total of 169 putative SGAs found in eight tomato accessions（Soianum lycopersicum, S.pimpinellifolium, S.cheesmaniae, S.chmielewskii, S.neorickii,S.peruvianum,S.habrochaites,S.pennellii） and four tissue types were used for correlation analysis.The results obtained in this study contribute annotation and classification of SGAs as well as detecting putative novel biosynthetic branch points.As such this represents a novel strategy for peak annotation for plant secondary metabolites.

关键词： Fruit ripening glycoalkaloids secondary metabolite Solanum lycopersicum tomato accessions

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Targeting Key Genes to Tailor Old and New Crops for a Greener Agriculture

引用

molecular plant 2020年第3期13卷 354-356页

作者： Alisdair R.Fernie Jianbing Yan Department of molecular physiology Max-Planck-lnstitute of Molecular Plant PhysiologyAm Muhlenberg 114476 PotsdamGermany National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China

Growing crops closer to the consumer is one of many approaches proposed to lessen the environmental footprint associated with food production(Banerjee and Adenauer,2014).This is complicated by an increasingly urban population,the lack of available land in cities and the consequent need for rapid crop cycling.Given these constraints,to date only lettuce and related leafy greens are currently cultivated at scale in urban farms(Touliatos et al.,2016).In recent years,there are increasing interests in the urban agriculture of fruits and berries,since controlled automated vertical farming systems offer the twin advantages of optimizing land use and the ease with which they can be designed to be more environmentally friendly and sustainable than traditional agriculture.However,a considerable challenge is that commercial crop varieties were historically,and still are,bred to be high yielding under typical field and greenhouse parameters.Moreover,given the perishable nature of fruits and berries,a reduced distance from farm to fork renders the shelf-life trait(and its associated detrimental effects on fruit quality)less imperative.Thus,the possibility to improve fruit and berry quality traits is a particularly attractive aspect of urban agriculture.In addition,the environmental benefit for these species is perhaps even greater than for other crops,since they are more often transported by air.A recent study uses multi-site CRISPR/Cas9-mediated genome editing to restructure vine line tomato plants into compact early-yielding plants suitable for urban agriculture(Figure 1),while demonstrating that a single gene editing strategy can produce a compact stature in the related plant groundcherry(Kwon et al.,2019).

关键词： crops yielding Figure

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Plasticity of Xyloglucan Composition in Bean （Phaseolus vulgaris）-Cultured Cells during Habituation and Dehabituation to Lethal Concentrations of Dichlobenil

引用

molecular plant 2010年第3期3卷 603-609页

作者： A. Alonso-Simon L. Neumetzler R Garcia-Angulo A.E. Encina J.L. Acebes J.M. Alvarez T. Hayashi Area de Fisiologia Vegetal Universidad de Leon E-24071 Leon Spain max planck institute of molecular plant physiology 14476 Golm Germany Kyoto University RISH Gokasho Uji Kyoto 611-0011 Japan

Bean cells that have been habituated to grow in a lethal concentration （12 μM） of 2,6-dichlorobenzonitrile （dichlobenil or DCB, a cellulose biosynthesis inhibitor） are known to have decreased cellulose content in their cell walls. Xyloglucan, which is bound to cellulose and together with it forms the main loading network of plant cell walls, has also been described to decrease in habituated cells, but whether the change on cellulose affects the xyloglucan structure besides its abundance has not been analyzed. Fragmentation analysis with xyloglucan-specific endoglucanase （XEG） and endocellulase revealed that habituation to DCB caused a change in the fine structure of xyloglucan, namely a decrease in fucosyl residues attached to the galactosyl-xylosyl residues along the glucan backbone. After the removal of herbicide from the medium （dehabituated cells）, xyloglucan recovered its fucosyl residues. In addition, some cello-oligosaccharides could be detected only in habituated cells＇ xyloglucan digested by XEG and endocellulase, corresponding to a glucan co- valently bound or co-precipitated with the hemicelluloses. These results show that structural flexibility of cell walls relies in part on the plasticity of xyloglucan composition and opens up new perspectives to further research in this field.

关键词： Bean 2,6-dichlorobenzonitrile habituation and dehabituation OLIMP （Oligosaccharides Mass Profiling） xyloglucan.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Stay-Green Not Always Stays Green

引用

molecular plant 2014年第8期7卷 1264-1266页

作者： Salma Balazadeh [a]University of Potsdam Institute of Biochemistry and Biology Karl-Liebknecht-Str. 24–25 Haus 20 14476 Potsdam-Golm Germany[b]Plant Signalling Group Max Planck Institute of Molecular Plant Physiology Am Mühlenberg 1 14476 Potsdam-Golm Germany

Senescence is a highly regulated recycling process in which nutrients are translocated from deteriorating leaves to newly developing leaves, flowers, seeds, or roots （Lim et al., 2007; Gregersen et al., 2013）. Chloroplast dismantling, a major event during senescence, involves the degradation of light-harvesting chlorophyll a/b-binding proteins （LHCPs） and the release of free chlorophyll （Chl） which is catabolized to phototoxic, cell-damaging Chl breakdown intermediates.

关键词： REGULATES CHLOROPHYLL DEGRADATION LEAF SENESCENCE HIGHER-plantS ARABIDOPSIS

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Exploring the genic resources underlying metabolites throughmGWASandmQTL inwheat: From large-scale gene identification and pathway elucidation to crop improvement

引用

plant Communications 2021年第4期2卷 127-139页

作者： Jie Chen Mingyun Xue Hongbo Liu Alisdair R.Fernie Wei Chen National Key Laboratory of Crop Genetic Improvement and National Center of plant Gene Research(Wuhan) Huazhong Agricultural UniversityWuhan 430070China College of plant Science and Technology Huazhong Agricultural UniversityWuhan 430070China max-planck-institute of molecular plant physiology Potsdam-Golm 14476Germany

Common wheat(Triticum aestivum L.)is a leading cereal crop,but has lagged behind with respect to the interpretation of the molecular mechanisms of phenotypes compared with other major cereal crops such as rice and maize.The recently available genome sequence of wheat affords the pre-requisite information for efficiently exploiting the potential molecular resources for decoding the genetic architecture of complex traits and identifying valuable breeding targets.Meanwhile,the successful application of metabolomics as an emergent large-scale profiling methodology in several species has demonstrated this approach to be accessible for reaching the above goals.One such productive avenue is combining metabolomics approaches with genetic designs.However,this trial is not as widespread as that for sequencing technologies,especially when the acquisition,understanding,and application of metabolic approaches in wheat populations remain more difficult and even arguably underutilized.In this review,we briefly introduce the techniques used in the acquisition of metabolomics data and their utility in large-scale identification of functional candidate genes.Considerable progress has been made in delivering improved varieties,suggesting that the inclusion of information concerning these metabolites and genes and metabolic pathways enables a more explicit understanding of phenotypic traits and,as such,this procedure could serve as an-omics-informed roadmap for executing similar improvement strategies in wheat and other species.

关键词： wheat metabolomics mGWAS mQTL pathway elucidation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：