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Response surface optimization of preparation parameters and evaluation of the antioxidant and antihypertensive activities of Spirulina protein hydrolysates

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Journal of Oceanology and Limnology 2025年第1期43卷 196-209页

作者： Qishan SUO Zhenzhen DENG Yang YUE Jing WANG Ning WU Lihua GENG Quanbin ZHANG CAS and Shandong Province Key Laboratory of Experimental marine Biology Center for Ocean Mega-ScienceInstitute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for marine Biology and biotechnology Qingdao Marine Science and Technology CenterQingdao 266237China University of Chinese Academy of Sciences Beijing 101408China

Safety,high protein content,and the numerous health benefits make Spirulina an outstanding source of bioactive *** from Spirulina,which exhibit antioxidant,anti-inflammatory,and antihypertensive effects,may assist in the management of cardiovascular diseases(CVDs).Here,we present the optimization of enzyme-digested hydrolysates derived from Spirulina *** using response surface methodology(RSM).We also evaluated the biological activity of Spirulina protein hydrolysates(SPHs)in contributing to the amelioration of hypertension and associated cardiovascular *** findings suggest that Spirulina protein extracts contain a complex proteinaceous composition with phycocyanin being the dominant protein,as evidenced by both SDS-PAGE and LC-MS/MS *** protease,protease K,andα-chymotrypsin exhibited a substantial hydrolytic effect on Spirulina protein,taking into account their degree of hydrolysis(DH),peptide yield,and molecular weight ***-factor experiments identified pH,temperature,and enzyme/substrate ratio as the key factors influencing ***,optimal hydrolysis conditions for the proteases were determined via RSM,leading to a considerable enhancement of the actual *** showed that SPHs have strong antioxidant activity against various free radicals,with a higher ability to scavenge hydroxyl ***,SPHs inhibited macrophage nitric oxide secretion and significantly inhibited angiotensin I-converting enzyme in vitro at 400μg/*** is worth noting that SPHs prepared with protease K exhibited a potent anti-hypertensive effect in *** findings indicate the potential usefulness of including dietary SPHs in preventing hypertension and associated *** research could be valuable in guiding the development of health-promoting foods within the Spirulina industry.

关键词： antioxidant activity antihypertensive effect angiotensin converting enzyme inhibition response surface methodology Spirulina

Genes and proteins expressed at different life cycle stages in the model protist Euplotes vannus revealed by both transcriptomic and proteomic approaches

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Science China(Life Sciences) 2025年第1期68卷 232-248页

作者： Yaohan Jiang Xiao Chen Chundi Wang Liping Lyu Saleh A.Al-Farraj Naomi A.Stover Feng Gao Key Laboratory of Evolution&marine Biodiversity(Ministry of Education)and Institute of Evolution&marine Biodiversity Ocean University of ChinaQingdao 266003China marine College Shandong UniversityWeihai 264209China Zoology Department College of ScienceKing Saud UniversityRiyadh 11451Saudi Arabia Department of Biology Bradley UniversityPeoria 61625USA Laboratory for marine Biology and biotechnology Qingdao Marine Science and Technology CenterQingdao 266237China

Sexual reproduction first appeared in unicellular protists and has continued to be an essential biological process in almost all *** protists, which contain both germline and somatic genomes within a single cell, have evolved a special form of sexual reproduction called conjugation that involves mitosis, meiosis, fertilization, nuclear differentiation, genome rearrangement, and the development of unique cellular structures. The molecular basis and mechanisms of conjugation vary dramatically among ciliates, and many details of the process and its regulation are still largely unknown. In order to better comprehend these processes and mechanisms from an evolutionary perspective, this study provides the first comprehensive overview of the transcriptome and proteome profiles during the entire life cycle of the newly-established marine model ciliate Euplotes vannus. Transcriptome analyses from 14 life cycle stages(three vegetative stages and 11 sexual stages) revealed over 26,000 genes that are specifically expressed at different stages, many of which are related to DNA replication,transcription, translation, mitosis, meiosis, nuclear differentiation, and/or genome rearrangement. Quantitative proteomic analyses identified 338 proteins with homologs associated with conjugation and/or somatic nuclear development in other ciliates, including dicer-like proteins, Hsp90 proteins, RNA polymerase Ⅱ and transcription elongation factors, ribosomal-associated proteins, and ubiquitin-related proteins. Four of these homologs belong to the PIWI family, each with different expression patterns identified and confirmed by RT-qPCR,which may function in small RNA-mediated genome rearrangement. Proteins involved in the nonhomologous end-joining pathway are induced early during meiosis and accumulate in the developing new somatic nucleus, where more than 80% of the germline sequences are eliminated from the somatic genome. A number of new candidate genes and proteins likely t

关键词： sexual reproduction transcriptomic proteomic genome rearrangement PIWI NHEJ

17α-methyltestosterone induced hepatocyte apoptosis through endoplasmic reticulum stress in Takifugu fasciatus

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Journal of Oceanology and Limnology 2025年第1期43卷 219-231页

作者： Xuanyin CHEN Sheng LUO Yanfang LI Rui LI Xiaojun LI Zhengyang HU Shaowu YIN Tao WANG College of marine Science and Engineering Jiangsu Province Engineering Research Center for Aquatic Animals Breeding and Green Efficient Aquacultural TechnologyNanjing Normal UniversityNanjing 210023China Co-Innovation center for marine Bio-Industry Technology of Jiangsu Province Lianyungang 222005China

17α-methyltestosterone(17α-MT)is an emerging pollutant,which is harmful to the endocrine system and reproduction of *** investigated the effects of different concentrations of 17α-MT(0,5,30,60,and 100 mg/kg)on endoplasmic reticulum stress(ERS)and apoptosis in the liver of Takifugu *** show that:(1)with the increase of 17α-MT treatment concentration,liver transaminases(alanine aminotransferase;aspartate aminotransferase)and the mRNA expression of ERS marker genes(glucose-regulated protein 78;calreticulin)of *** were significantly increased compared with the control group(P<0.05);(2)the activity of succinate dehydrogenase(SDH),Caspase3 and Caspase9 in the liver of *** increased with the increase of 17α-MT concentration compared with the control group(P<0.05);(3)by using 4-phenylbutyricacid(4-PBA)inhibitors to stimulate ERS through in vitro experiments,the expression of ERS and apoptosis-related genes significantly decreased(P<0.05),and the apoptosis rate of *** hepatocytes was significantly inhibited(P<0.05)under 17α-MT *** study confirmed that ERS played an important role in the induction of apoptosis in the hepatocytes of ***,which enriched the ecotoxicological information of environmental androgens.

关键词： 17α-methyltestosterone Takifugu fasciatus liver endoplasmic reticulum stress apoptosis

Characterization and genomic analysis of Pseudoalteromonas phage v B_Pal P_Y7,representing a novel viral genus,Miuvirus

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Journal of Oceanology and Limnology 2025年第1期43卷 299-312页

作者： Miaolan WU Hongmin WANG Ye MI Yantao LIANG Kaiyang ZHENG Yundan LIU Yue DONG Linyi REN Yue SU Hongbing SHAO Yeong Yik SUNG Wen Jye MOK Li Lian WONG Min WANG College of marine Life Sciences Institute of Evolution and Marine BiodiversityMoE Laboratory of Evolution and Marine BiodiversityFrontiers Science Center for Deep Ocean Multispheres and Earth SystemCenter for Ocean Carbon NeutralityOcean University of ChinaQingdao 266100China Qingdao City center for Disease Control and Prevention Qingdao Institute of Prevention MedicineQingdao 266033China UMT-OUC Joint Centre for marine Studies Qingdao 266100China Institute of marine biotechnology Universiti Malaysia TerengganuKuala Terengganu 21030Malaysia Haide College Ocean University of ChinaQingdao 266100China The Affiliated Hospital of Qingdao University Qingdao 266000China

Pseudoalteromonas is a group of marine bacteria widespread in diverse marine sediments,producing a wide range of bioactive ***,only a limited number of Pseudoalteromonas phages have been isolated and *** this study,a novel lytic Pseudoalteromonas phage,denoted as vB_PalP_Y7,was isolated from sewage samples collected at the Seafood Market in Qingdao,***_PalP_Y7 remained stable across a wide range of temperatures(-20–50℃)and a wide pH range(3–12).The vB_PalP_Y7 phage harbors a linear double-stranded DNA molecule of 57699 base pairs(bp)with a G+C content of 45.90%.Furthermore,it is predicted to contain 58 open reading frames(ORFs).Phylogenetic analysis and protein network relationship analysis revealed low similarity between vB_PalP_Y7 and viruses in the ICTV and IMG/VR4 database,suggesting that vB_PalP_Y7 may be a potential new genus,*** study contributed valuable insights to comprehend the relationship between Pseudoalteromonas phages and their host organisms.

关键词： Pseudoalteromonas phage genomic analysis phylogenetic analysis viral cluster

Ferulic acid alleviates long-term avermectin-induced damage to the spleen of carp and restores its inflammatory response and oxidative balance

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Journal of Environmental Sciences 2025年第5期151卷 616-626页

作者： Hanyu Wang Shenye Feng Enzhuang Pan Xiaomeng Ji Mengyuan Zhou Shuai Zhang Baoshi Xu Huimiao Feng Jilei Yin Zibo Dong Jiangsu Key Laboratory of marine Bioresources and Environment Co-Innovation Center of Jiangsu Marine Bio-industry TechnologyJiangsu Marine Pharmaceutical Resources Development Engineering Research CenterJiangsu Key Laboratory of Marine Pharmaceutical Compound ScreeningCollege of PharmacyJiangsu Ocean UniversityLianyungang 222005China Department of Traditional Chinese Medicine Jiangsu Union Technical Institute Lianyungang Branch institute of Traditional Chinese MedicineLianyungang 222006China

Extensive use of avermectin(AVM)in agriculture can seriously contaminate fish in *** acid(FA)is a strong antioxidant sourced from plants and is widely applied in food and medicine,but its protective function on the immunological damage caused by AVM is still *** this study,carp were chronically exposed to(1/1096 hr LC_(50))AVM for 30 day(s),with a dietary supplement of FA(400 mg/kg)to assess its effects on carp *** experimental groups(n=10)included:control,AVM-exposed(2.404μg/L),FA+AVM co-treated,and FA ***-term AVM exposure altered spleen tissue,reducing serum complement C3(p<0.01)and immunoglobulin M levels(p<0.001),and increasing proinflammatory tnf-α(p<0.001),il-6(p<0.001),il-1β(p<0.001),and inos mRNA levels,whilst down-regulating the anti-inflammatory tgf-β(p<0.05).Additionally,it disrupted the balance of oxidative stress indicators such asMDA(p<0.001),T-AOC,GSH,and CAT,leading to spleen tissue apoptosis(42.4%).However,the addition of FA reversed these conditions,elevated the anti-inflammatory factor,and improved spleen immune function following chronic exposure to AVMin ***,the ability to restore oxidative homeostasis in carp by adjusting the Nrf2/NQO-1 axis protected the health of spleen *** discovery also supports the development of fish feed for aquaculture.

关键词： Avermectin Ferulic acid Oxidative stress Inflammation response

Adsorption Characteristics of Congo Red Dye onto Calcinated Mytilus edulis Shell Powders

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Journal of Ocean University of China 2025年第1期24卷 229-237页

作者： WANG Xin GE Xiangyun ZHU Siqi LIU Weixiang XING Ronge LI Pengcheng LI Kecheng marine Science and Engineering College Qingdao Agricultural UniversityQingdao 266109China Key Laboratory of Experimental marine Biology Center for Ocean Mega-ScienceInstitute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for marine Drugs and Bioproducts Qingdao Marine Science and Technology CenterQingdao 266237China

Dye wastewater poses a significant threat to aquatic organisms due to its high *** or eliminating the dye waste from the water is necessary for a healthy and sustainable *** study investigated the adsorption properties of Congo red dye on Mytilus edulis shell powders prepared by calcination at 500℃,700℃,and 900℃.The modified shell powder products were analyzed by SEM(scanning eletron microscopy)and FTIR(fourier transform infrared spectroscopy)for the morphology and structural *** effects of different calcination temperatures,reaction times,reaction temperatures,and initial concentration of Congo red on the adsorption properties were *** adsorption kinetics and isothermal adsorption models were also *** results revealed that the shell powder calcinated at 900℃showed the best adsorption capacity on Congo red from aqueous *** adsorption reaction reached equilibrium after 150 min and followed by the pseudo-second-order kinetic *** 25℃,96.2%of the Congo red in the solution could be removed,and the adsorption capacity could reach at least 1015 mg g^(–1).The adsorption isotherm is fit with the Freundlich model,indicating a multiphase adsorption *** results are helpful for cleaning and treating printing and dyeing effluents as well as high-value utilization of shell waste resources.

关键词： adsorption Congo red high-temperature calcination Mytilus edulis shell

The potential of Ca isotopes to trace subducted marine carbonates in deep mantle

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Journal of Oceanology and Limnology 2025年第1期43卷 29-40页

作者： Hongli ZHU Renqiang LIAO Long DU Zhaofeng ZHANG Key Laboratory of Ocean Observation and Forecasting Center of Deep Sea ResearchInstitute of OceanographyChinese Academy of SciencesQingdao 266071China Laboratory for marine Geology Qingdao Marine Science and Technology CenterQingdao 266237China College of Earth Science and Engineering Shandong University of Science and TechnologyQingdao 266590China research center for Planetary Science College of Earth SciencesChengdu University of TechnologyChengdu 610059China

marine carbonates,the major carrier of carbon upon the upper crust,can be subducted into the Earth’s interior along with oceanic crust,and then returned to the surface through magmatism,which constitute the deep carbon *** process plays an important role in modulating the CO_(2) concentrations in the atmosphere over geologic time,and thus the forming of the habitable ***,identifying recycled marine carbonates in the mantle is critical to well understand the global deep carbon *** is one of the major constituent cations in marine carbonates and its isotopes may be a potential tracer for recycled marine carbonates in the *** further evaluate the capability and challenges of Ca isotopes as such a geochemical tracer,we reviewed the Ca isotopic compositions in important reservoirs and the behavior of Ca isotopes during high-temperature geological processes that are related to the deep carbon cycle,including plate subduction,mantle metasomatism,mantle partial melting,magma differentiation,*** studies show that carbonate-rich marine sediments have significantly lowerδ^(44/40) Ca than the Earth mantle,and metasomatism by such recycled materials can cause lighter Ca isotopic compositions in deep mantle-derived rocks than those of the depleted mantle and mid ocean ridge ***,the Ca isotopic fractionation during partial melting of mantle peridotites is small(~0.10‰)and the Ca isotopic fractionation during plate subduction and intermediate-mafic magma evolution is *** investigations suggest that Ca isotopes have great advances in tracing such recycled materials in the ***,other processes(such as the influence by partial melts of eclogites)may induce similar effects on mantle-derived rocks as subducted marine carbonates but still remains debated,and thus further investigations are strongly needed in the future.

关键词： Ca isotope marine carbonate mantle metasomatism partial melting fractional crystallization

Morphology and phylogeny of two species of Bathypathes from the tropical western Pacific,with description of a new species

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Journal of Oceanology and Limnology 2025年第1期43卷 248-260页

作者： Ting LÜ Zifeng ZHAN Kuidong XU Laboratory of marine Organism Taxonomy and Phylogeny Qingdao Key Laboratory of Marine Biodiversity and ConservationInstitute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for marine Biology and biotechnology Laoshan LaboratoryQingdao 266237China University of Chinese Academy of Sciences Beijing 100049China

The black coral genus Bathypathes Brook,1889 represents a relatively diverse and ecologically important assemblage comprising the deep-sea hard substrate *** phylogenetic investigations have revealed the polyphyletic nature of Bathypathes,urging further morphological analysis to understand its *** combing morphological and molecular data,we describe Bathypathes multifurcata *** report *** Molodtsova,Opresko&Wagner,2022based on the specimens collected from the tropical western *** phylogenetic topology reconstructed using nuclear ITS1-5.8S-ITS2 and three mitochondrial fragments suggests the separation of Bathypathes into four *** analysis of morphological characters,particularly the colony branch and pinnule patterns also roughly classify Bathypathes into four morphological groups A–*** A is characterized by the unbranched colony and the subopposite arrangement of *** contrast to Group A,Groups B–D have the alternately arranged *** these,Group C exhibits a distinctly long unpinnulated stem compared to Groups B and *** B has unbranched colony,relatively short unpinnulated stem,and spines on pinnules have blunt *** Group D,large colonies(higher than 40 cm)have one or a few branches,small colonies(less than 40 cm)are unbranched,and spines on pinnules have acute *** data particularly those species without sequences are necessary to verify the classification of these groups.

关键词： Antipatharia black coral taxonomy phylogeny seamount western Pacific

Genomic analysis of modern maize inbred lines reveals diversity and selective breeding effects

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Science China(Life Sciences) 2025年第2期68卷 578-581页

作者： Lisen Li Deqian Fan Chenglong Zhu Zhuoya Liu Wenji Huang Peidong Xin Huishan Yue Mengying Li Yufei Wang Wenjie Xu Jiangmin Zheng Ye Li Ziwei Yu Jianzhong Ling Qiang Qiu Wen Wang Chenguang Feng Xiaojing Song Kun Wang Shaanxi Key Laboratory of Qinling Ecological Intelligent Monitoring and Protection School of Ecology and EnvironmentNorthwestern Polytechnical UniversityXi’an 710072China Shanghai Fisheries research Institute Shanghai Fisheries Technical Extension StationShanghai 200433China East China Sea Fisheries research Institute Chinese Academy of Fishery SciencesShanghai 200090China Laboratory for marine Biology and biotechnology Qingdao Marine Science and Technology CenterQingdao 266237China

Dear Editor,Maize(Zea mays L.)is one of the most widely cultivated crops worldwide(Ranum et al.,2014),accounting for about 37.2%of the global cereal crop *** production yield has increased over sevenfold since single-... 详细信息

关键词： breeding crops cultivated