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Uncovering the immunological repertoire of the carpet shell clam Ruditapes decussatus through a transcriptomic-based approach

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Aquaculture and fisheries 2019年第1期4卷 37-42页

作者： Frederico M.Batista Allison M.Churcher Manuel Manchado Alexandra Leitao Deborah M.Power Centro de Cieencias do Mar(CCMAR) Universidade do AlgarveCampus de Gambelas8005-139 FaroPortugal Instituto Portugues do Mar e da Atmosfera Estaçao Experimental de Moluscicultura de TaviraPortugal department of Biosciences College of ScienceSwansea UniversitySwanseaSA28PPWalesUnited Kingdom IFAPA Centro El Toruno Junta de AndalucíaCamino Tiro Pichon sn11500 El Puerto de Santa MaríaCadizSpain Environmental Science Center(ESC) Qatar UniversityP.O.Box 2713 DohaQatar

The grooved carpet-shell clam Ruditapes decussatus is native to the Northern Atlantic and Mediterranean Sea and has a high commercial *** is one of the main native bivalve species cultured in *** main objective of the present study was to gain further insights into the immunological repertoire of *** through a transcriptomic *** mantle samples of eight *** individuals were sequenced using Illumina platform.A total of 67132 contigs with more than 800 bp were *** annotation of these contigs revealed 146 immune-related *** gene families in which the highest number of immune-related genes was observed were:C1q domain-containing proteins(63),tumor necrosis factors(15)and toll-like receptors(TLRs,10).A total of 5359 putative single nucleotide polymorphisms(SNPs)were identified in the 146 immune-related *** density of SNPs ranged between 0.04 and 7.92 SNPs/100 *** highest and the lowest SNP density were observed in genes of the C1q domain-containing protein *** to the importance of TLRs in innate immunity,we focused our attention on these membrane *** TLRs were identified based on protein domain *** analysis revealed that *** TLRs were diverse and only 3 showed orthology with TLRs of known immune functions in other bivalve ***,our analysis suggests that lineage restricted-expansions of TLRs occurred in all mollusc taxa analysed including in venerids.

关键词： Ruditapes decussatus Transcriptome Immune genes Toll-like receptors

甲壳动物大颚器及其合成甲基法尼酯的放射化学测定法

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水产科学 2009年第2期28卷 113-116页

作者：陆剑锋常国亮吴旭干白桦赵维信成永旭合肥工业大学生物与食品工程学院安徽合肥230009 上海海洋大学生命科学与技术学院上海200090 department of Entomology University of Kentucky Lexington

放射化学(Radiochemistry)主要是研究放射性物质和原子核转变过程的化学分支学科[1].放射化学与原子核物理对应地关联和交织在一起,成为科学技术的两个兄弟学科.

关键词：甲壳动物激素大颚器甲基法尼酯

Current Development and Prospects of Pond Smelt （Hypomesus olidus） Farming Industry in China

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Asian Agricultural Research 2018年第11期10卷 62-67页

作者： Qingyu CHAI Fangying CHAI Ting YU Yongfu JU Hongxian YU Northeast Forestry University Harbin 150000 China Heilongjiang Provincial Water Resources department Harbin 150000 China

The pond smell (Hypomesus olidus) , originally transplanted from North Korea, was introduced to China in 1938 and gradually distributed to various areas of China. The pond smelt was the first fish species inlnxluced to the water of China. It not only has the function of protecting water quality, and also has high economic and nutritional values which are helpful for diversifying the fishery productive activities of China. The present review analyzed the transplantation and production status of pond smelt in China, deseribed the key situations and factors influencing the establishment and current level of aquaculture development. Since its introduction, the pond smelt transplantation in China was distributed in Northeast of China. From the 1980s, reservoirs and lakes all over China began to transplant pond smelt, and the transplantation of pond smelt went into the outbreak period. In order to improve the efficiency of transplantation, scientific and technical personnel studied the simple and practical technology of artificial breeding and transplantation, the medicine for preventing and controlling sapmlegniasis which often leads to death of fertilized eggs. To meet the demand of market, more and more pond smelt processing products have been studied and developed, such as grilled, milk flavor, fish sauce, ham-sausage, and brew vinegar of pond smelt. The pond smelt farming in China has broad prospects development, yet there are also challenges to overcome. It is recommended to take proper policies for pond smelt development, strengthen pond smelt scientific research, transplantation technology training, establish pond smelt fisheries association, support dominant enterprises, and implement brand strategy. [ABSTRACT FROM AUTHOR]

关键词：池塘人工育种农业种植技术

Retail Seafood Waste Prevention: Reducing Retail and Consumer Fresh-Fish Waste by Cooking Directly from Frozen

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Food and Nutrition Sciences 2021年第3期12卷 290-307页

作者： Thomas O’Donnell Solomon H. Katz Alexandra Romey Benjamin Fulton Linda Croskey Pete Pearson Jonathan Deutsch Land Chemicals and Redevelopment Division U.S. Environmental Protection Agency Philadelphia PA USA W. M. Krogman Center for Research on Child Growth and Development at the University of Pennsylvania Philadelphia PA USA Drexel Food Lab Drexel University Philadelphia PA USA department of Food and Hospitality Management Drexel University Philadelphia PA USA World Wildlife Fund Washington DC USA

More than 30% of fish caught from the world’s oceans are never eaten by consumers adding a significant but unnecessary strain to the sustainability of global fisheries. Although a lot of this loss occurs at sea, in developed countries, a significant amount happens at retail stores and in households. People can help with this problem if they find and use new ways to interact with their retailers and with the way they store and cook seafood at home. Consumers can primarily purchase seafood that has never been frozen, was previously frozen, or is still frozen. Nearly all retail waste occurs when consumers do not buy seafood within a few days after it is in the unfrozen, display cases, forcing the stores to dispose of the fish in landfills or sewage plants. An estimated 220 million 4-ounce meal portions of the most popular seafood in the United States including shrimp, salmon, and cod meet this fate. This number can be reduced to the direct extent consumers can be persuaded to buy and cook from frozen. Retailers are motivated to sell more frozen seafood because profit margins are reported to be higher and labor and disposal costs are lower. Many stores also benefit from their brand’s sustainability image, and contributions to corporate, national, and international waste reduction goals. Their challenge has been to educate and encourage consumers to choose frozen before fresh. Taste-testing evidence gathered in this study demonstrated that consumers could adopt easy, new culinary skills to cook seafood from frozen that tastes just as good as what they are used to. Our studies also highlighted other consumer benefits, including: less fish handling required, simple preparation, easier mea

关键词： Seafood Waste Cooking fisheries Consumers Retail

Who eats whom,when and why?Juvenile cannibalism in fish Asian seabass

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Aquaculture and fisheries 2017年第1期2卷 1-9页

作者： Xiaojun Liu Junhong Xia Hongyan Pang Genhua Yue Key Laboratory of Freshwater Aquatic Genetic Resources Shanghai Ocean UniversityMinistry of AgricultureShanghai 201306China Molecular Population Genetics&Breeding Group Temasek Life Sciences Laboratory1 Research LinkNational University of SingaporeSingapore 117604Singapore State Key Laboratory of Biocontrol Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic AnimalsCollege of Life SciencesSun Yat-Sen UniversityGuangzhouGuangdong 510275China department of Biological Sciences National University of SingaporeSingapore 117543Singapore School of Biological Sciences Nanyang Technological University60 Nanyang DriveSingapore 637551Singapore

While juvenile cannibalism plays an important role in the evolution of organisms in natural populations,it is a serious problem in aquaculture.A number of genetic and environmental factors result in different rates of *** there is kin recognition in juvenile cannibalism in fish is poorly *** studied cannibalism and kinship recognition in juveniles of Asian seabass using molecular parentage analysis with polymorphic *** the three mass crosses,under an ordinary feeding scheme without size grading,the rate of juvenile loss due to cannibalism was 1.08% per *** the group without feeding for 24 h,2.30%±0.43% of offspring per day were lost within 24 h due to *** detected that juveniles avoided cannibalizing their siblings when they were not hungry,whereas cannibalism among siblings increased when they were *** data suggest that there is kin discrimination in fish *** genetically closely related offspring in the same tanks and appropriate levels of feeding may reduce the rate of *** hypothesized that the chemical cues for kin discrimination might be secreted by fish *** test this hypothesis,we analyzed gene expression profiles in the skins of juveniles under slightly and very hungry conditions using RNA sequencing and bioinformatics *** differently expressed under slightly and very hungry conditions were *** them,genes from the trypsin family were significantly downregulated under starved conditions,suggesting that they may play a role in kin discrimination.

关键词： Fish Cannibalism Kinship RNA-seq Gene expression

Design of Vibrio 16S rRNA Gene Specific Primers and Their Application in the Analysis of Seawater Vibrio Community

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Journal of Ocean University of China 2006年第2期5卷 157-164页

作者： LIU Yong YANG Guanpin WANG Hualei CHEN Jixiang SHI Xianming ZOU Guiwei WEI Qiwei SUN Xiuqin College of Marine Life Sciences Ocean University of China Qingdao 266003 P.R. China department of Food Science and Technology Shanghai Jiaotong University Shanghai 201101 P.R. China Yangtse River Fishery Institute Chinese Academy of Fishery Jingzhou 434000 P.R. China The First Institute of Oceanography National Oceanic Administration of China Qingdao 266061 P.R. China

The pathogenic species of genus Vibrio cause vibriosis, one of the most prevalent diseases of maricultured animals and seafood consumers. Monitoring their kinetics in the chain of seafood production, processing and consumption is of great importance for food and mariculture safety. In order to enrich Vibrio-representing 16S ribosomal RNA gene （rDNA） fragments and identify these bacteria further real-timely and synchronously among bacterial flora in the chain, a pair of primers that selectively amplify Vibrio 16S rDNA fragments were designed with their specificities and coverage testified in the analysis of seawater Vibrio community. The specificities and coverage of two primers, VF169 and VR744, were determined theoretically among bacterial 16S rDNAs available in GenBank by using BLAST program and practically by amplifying Vibrio 16S rDNA fragments from seawater DNA. More than 88.3% of sequences in GenBank, which showed identical matches with VR744, belong to Vibrio genus. A total of 33 clones were randomly selected and sequenced. All of the sequences showed their highest similarities to and clustered around those of diverse known Vibrio species. The primers designed are capable of retrieving a wide range of Vibrio 16S rDNA fragments specifically among bacterial flora in seawater, the most important natural environment of seafood cultivation.

关键词： Vibrio vibriosis bacterial community primer

Zooplankton Diversity of a Soft-water and Highly De-mineralized Reservoir of Meghalaya(Northeast India):The Spatio-temporal Variations and Influence of Abiotic Factors

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Journal of fisheries Science 2021年第2期3卷 19-35页

作者： Bhushan Kumar Sharma Sumita Sharma department of Zoology North-Eastern Hill UniversityShillongMeghalaya793022India Lady Veronica Road ShillongMeghalaya793003India

Hydrobiological survey of a‘soft-water’and‘highly de-mineralized’reservoir of Meghalaya state of northeast India is undertaken to analyze zooplankton diversity with reference to the spatio-temporal variations and influence of abiotic *** littoral and limnetic zooplankton assemblages of this subtropical reservoir without aquatic vegetation reveal total 36 species,and record lower abundance,quantitative dominance of Rotifera,sub-dominance of Cladocera and Copepoda and moderate species *** cochlearis,Bosmina longirostris,Polyarthra vulgaris,Mesocyclops leuckarti,Conochilus unicornis and Asplanchna priodonta influence abundance,species diversity,dominance and equitability of *** report differential spatial influence of individual abiotic factors with the relatively more importance at the limnetic region,and the canonical correspondence analysis registers 72.5%and 78.8%cumulative influence of 10 abiotic factors on the littoral and limnetic assemblages,*** spatial differences of various diversity aspects and the influence of abiotic factors suggest habitat heterogeneity amongst the two *** study is a useful contribution to zooplankton diversity of the subtropical environs,and soft and de-mineralized waters in *** results mark a distinct contrast to the lowest richness and abundance of zooplankton noted from India vide the preliminary 1990-91 survey of this reservoir.

关键词： De-mineralized Diversity Rainwater-fed Very soft-water Subtropical reservoir

Molecular cloning of partial 14-3-3 genes in the marine sponge Hymeniacidon perleve and its role in differentiating infectious and non-infectious bacteria

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Science China(Technological Sciences) 2013年第3期56卷 766-776页

作者： FU WanTao ZHANG JuLin ZHENG ChangBo LIU Jing AN ZhongFu LIU HongWen ZHANG Wei Institute of Marine Science-Technology and Environment Dalian Ocean University Marine Bioproducts Engineering Group Dalian Institute of Chemical PhysicsChinese Academy of Sciences Key Laboratory of Nearshore Marine Environmental Science and Technology of Liaoning Higher Education Graduate School Dalian Ocean University Vocation & Technological College Dalian Ocean University Dalian Environmental Monitoring Center Flinders Centre for Marine Bioproducts Development and Department of Medical BiotechnologySchool of MedicineFlinders University

Sponges （phylum Porifera） are the phylogenetically oldest metazoa and highly efficient filter feeders. In the marine ecosystem, they are unconditionally exposed to environmental stresses. Understanding the sponge-bacteria interaction is hence of both eco- logical and biological significance. This study investigated the specific interaction between the sponge Hymeniacidon perleve and the non-infectious bacteria, Escherichia coli and infectious bacteria, Vibrio spp. by measuring the 14-3-3 mRNA expression of H. perleve. Three partial cDNAs of 14-3-3 proteins and partial 18S RNA in H. perleve were cloned and sequenced. Using Re- verse-transcription real-time PCR, the 14-3-3 mRNA expression of H. perleve was examined when exposed to three common bacteria in aquatic water--E, coli and two Vibrio spp. for different time and dosages. H. perleve could efficiently remove E. coli from the water column without self-infection; however Vibrio at higher dosages infected H. perleve. When H. perleve was ex- posed to E. coli （1.1×10^7 CFU mL^-1）, V. anguillarum II （1.2×10^6 CFU mL^-1） and V. alginolyticus （3.6×10^5 CFU mL-1） for 6 h, the 14-3-3 mRNA expression in the V. anguillarum II and V. alginolyticus groups was down-regulated by 2.67- and 2.36-fold, respectively. The 14-3-3 mRNA expression in the E. coli group was not significantly different. However, no clear trend was ob- served on the 14-3-3 transcript levels of H. perleve in response to different doses of V. anguillarum II for different time. The re- sults demonstrated that infectious bacteria can be discriminated by 14-3-3 mRNA expression of sponge H. perleve.

关键词： marine sponge Hymeniacidon perleve 14-3-3 mRNA RT-PCR infectious bacteria

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贝类壳-体质量比和静水沉降特性的相关性

生态学报 2018年第18期38卷 6778-6785页

作者：罗渡王学杰徐猛顾党恩牟希东韦慧杨叶欣 Cesar G.Demayo 胡隐昌农业农村部休闲渔业重点实验室广东省现代休闲渔业工程技术研究中心中国水产科学研究院珠江水产研究所广州510380 department of Biological Sciences College of Science and MathematicsMindanao State University-Iligan Institute of TechnologyIligan CityPhilippines

软体动物贝壳表型是适应性进化的结果,为探究贝壳形态特性与软体动物随水流扩散功能的相关性,实验选取9种具代表性的腹足纲和双壳纲贝类作为研究对象,研究了其成体的壳-体质量比与静水沉降特性及二者的相关性。结果表明:体质量的变异系... 详细信息

软体动物贝壳表型是适应性进化的结果,为探究贝壳形态特性与软体动物随水流扩散功能的相关性,实验选取9种具代表性的腹足纲和双壳纲贝类作为研究对象,研究了其成体的壳-体质量比与静水沉降特性及二者的相关性。结果表明:体质量的变异系数(C.V.=1.11)大于壳长(C.V.=0.67)、壳宽(C.V.=0.54)的变异系数;壳-体质量比具较强的种属特异性,种内变异系数小(C.V.=0.20),而种间差异极显著(P<0.01)。受试贝类的平均壳-体质量比为0.32±0.13,陆生贝类的壳-体质量比显著小于水生贝类(P<0.01),壳-体质量比最大的河蚬(0.54±0.06)是最小的非洲大蜗牛(0.17±0.04)的3倍,福寿螺的壳-体质量比为0.50±0.06。双壳类和螺类的沉降行为差异明显,只有中华园田螺、铜锈环棱螺和河蚬不能在静水中漂浮;沉降速度最大的河蚬((24.99±4.22) cm/s)是最小的椭圆萝卜螺((4.13±0.96) cm/s)的6倍,入侵种非洲大蜗牛((18.30±3.64) cm/s)和福寿螺((21.77±5.23) cm/s)与土著种铜锈环棱螺((19.48±3.14) cm/s)和中华园田螺((21.44±3.92) cm/s)在沉降速度上无显著差异(P<0.01)。成体贝类的壳-体质量比与沉降速度(R2=0.28)、沉降加速度(R2=0.39)之间存在一定的相关性。在此基础上,贝类沉降特性随生活史变化的研究将进一步揭示贝类进化与种群扩散中的生态水力学作用。

关键词：福寿螺非洲大蜗牛生物入侵生态水力学种群扩散