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Evolution and host-specific adaptation of Pseudomonas aeruginosa

四川生理科学杂志 2024年第7期46卷 1579-1579页

作者： Aaron Weimann Victor Phillip Dahdaleh Heart & Lung Research Institute University of Cambridge Cambridge UK university of Cambridge Molecular Immunity Unit MRC Laboratory of Molecular Biology Cambridge UK Cambridge Centre for AI in Medicine University of Cambridge Cambridge UK Department of Veterinary Medicine University of Cambridge Cambridge UK Victor Phillip Dahdaleh Heart & Lung Research Institute University of Cambridge Cambridge UK university of Cambridge Molecular Immunity Unit MRC Laboratory of Molecular Biology Cambridge UK Cambridge Centre for AI in Medicine University of Cambridge Cambridge UK Laboratory of Pathogens and Host Immunity (LPHI) UMR5235 CNRS/Université de Montpellier Montpellier France Victor Phillip Dahdaleh Heart & Lung Research Institute University of Cambridge Cambridge UK university of Cambridge Molecular Immunity Unit MRC Laboratory of Molecular Biology Cambridge UK Cambridge Centre for Lung Infection Royal Papworth Hospital Cambridge UK Victor Phillip Dahdaleh Heart & Lung Research Institute University of Cambridge Cambridge UK university of Cambridge Molecular Immunity Unit MRC Laboratory of Molecular Biology Cambridge UK Department of Physiology Bioscience Institute University College Cork Cork Ireland university of Cambridge Molecular Immunity Unit MRC Laboratory of Molecular Biology Cambridge UK university of Cambridge Molecular Immunity Unit MRC Laboratory of Molecular Biology Cambridge UK Cambridge Centre for Lung Infection Royal Papworth Hospital Cambridge UK Victor Phillip Dahdaleh Heart & Lung Research Institute University of Cambridge Cambridge UK Department of Biochemistry University of Cambridge Cambridge UK Department of Medicine University of Cambridge Cambridge UK Department of Biochemistry University of Cambridge Cambridge UK Department of Medicine University of Cambridge Cambridge UK Wellcome Sanger Institute Hinxton UK Department of Medicine University of Cambridge Cambridge UK Cambridge Unive

The major human bacterial pathogen Pseudomonas aeruginosa causes multidrug-resistant infections in people with underlying immunodeficiencies or structural lung diseases such as cystic fibrosis(CF).We show that a few environmental isolates,driven by horizontal gene acquisition,have become dominant epidemic clones that have sequentially emerged and spread through global transmission networks over the past 200 years.

关键词： lung diseases sequentially

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Microbial electrosynthesis of acetate from CO_(2)in three-chamber cells with gas diffusion biocathode under moderate saline conditions

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Environmental Science and Ecotechnology 2023年第4期16卷 78-87页

作者： Paolo Dessì Claribel Buenano-Vargas Santiago Martínez-Sosa Simon Mills Anna Trego Umer ZIjaz Deepak Pant Sebastia Puig Vincent O'Flaherty Pau Farras School of Biological and Chemical Sciences and Energy Research Centre Ryan InstituteUniversity of GalwayUniversity RoadH91 CF50GalwayIreland LEQUiA Institute of the EnvironmentUniversity of GironaCarrer Maria Aurelia Capmany 69E-17003GironaSpain Microbiology Department School of Natural SciencesUniversity of GalwayUniversity RoadH91 TK33GalwayIreland Infrastructure and Environment Research Division School of EngineeringUniversity of GlasgowGlasgowUnited Kingdom Separation and Conversion Technology Flemish Institute for Technological Research(VITO)Boeretang 2002400MolBelgium

The industrial adoption of microbial electrosynthesis(MES)is hindered by high overpotentials deriving from low electrolyte conductivity and inefficient cell *** this study,a mixed microbial consortium originating from an anaerobic digester operated under saline conditions(∼13 g L^(−1)NaCl)was adapted for acetate production from bicarbonate in galvanostatic(0.25 mA cm^(−2))H-type cells at 5,10,15,or 20 g L^(−1)NaCl *** acetogenic communities were successfully enriched only at 5 and 10 g L^(−1)NaCl,revealing an inhibitory threshold of about 6 g L^(−1)Na^(+).The enriched planktonic communities were then used as inoculum for 3D printed,three-chamber cells equipped with a gas diffusion *** cells were fed with CO_(2)gas and operated galvanostatically(0.25 or 1.00 mA cm^(−2)).The highest production rate of 55.4 g m^(−2) d^(−1)(0.89 g L^(−1)d^(−1)),with 82.4%Coulombic efficiency,was obtained at 5 g L^(−1)NaCl concentration and 1 mA cm^(−2)applied current,achieving an average acetate production of 44.7 kg MWh−*** electron microscopy and 16S rRNA sequencing analysis confirmed the formation of a cathodic biofilm dominated by Acetobacterium ***,three 3D printed cells were hydraulically connected in series to simulate an MES stack,achieving three-fold production rates than with the single cell at 0.25 mA cm^(−2).This confirms that three-chamber MES cells are an efficient and scalable technology for CO_(2)bio-electro recycling to acetate and that moderate saline conditions(5 g L^(−1)NaCl)can help reduce their power demand while preserving the activity of acetogens.

关键词： Acetobacterium Bioelectrochemical system Conductivity Electrochemical cell design Gas diffusion electrode

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Direct synthesis of large-area Al-doped graphene by chemical vapor deposition:Advancing the substitutionally doped graphene family

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Nano Research 2022年第2期15卷 1310-1318页

作者： Sami Ullah Yu Liu maria Hasan Wenwen Zeng Qitao Shi Xiaoqin Yang Lei Fu Huy Q.Ta Xueyu Lian Jingyu Sun Ruizhi Yang Lijun Liu Mark H.Rummeli College of Energy Soochow Institute for Energy and Materials Innovations Soochow UniversitySuzhou 215006China Key Laboratory of Advanced Carbon Materials and Wearable Energy Technologies of Jiangsu Province Soochow UniversitySuzhou 215006China NUTECH School of Applied Sciences&Humanities and Computer Engineering Department National University of TechnologyIslamabad 42000Pakistan Centre of Polymer and Carbon Materials Polish Academy of SciencesM.Curie-Sklodowskiej 34Zabrze 41-819Poland School of Energy and Power Engineering Xian Jiaotong UniversityXian 710049China College of Chemistry and Molecular Science Wuhan UniversityWuhan 430072China Institute for Complex Materials IFW Dresden 20 Helmholtz Strasse Dresden 01069Germany Institute of Environmental Technology VSB-Technical University of Ostrava17.listopadu 15Ostrava 70833Czech Republic

Graphene doping continues to gather momentum because it enables graphene properties to be tuned,thereby affording new properties to,improve the performance of,and expand the application potential of *** can be chemically doped using various methods such as surface functionalization,hybrid composites(e.g.,nanoparticle decoration),and substitution doping,wherein C atoms are replaced by foreign ones in the graphene *** works have predicted that graphene could be substitutionally doped by aluminum(Al)atoms,which could hold promise for exciting applications,including hydrogen storage and evolution,and *** theoretical predictions suggest that Al substitutionally doped graphene(AIG)could serve as a material for gas sensors and the catalytic decomposition of undesirable ***,fabricating Al substitutionally doped graphene has proven challenging until ***,we demonstrate how controlled-flow chemical vapor deposition(CVD)implementing a simple solid precursor can yield high-quality and large-area monolayer AIG,and this synthesis is unequivocally confirmed using various characterization methods including local electron energy-loss spectroscopy(EELS).Detailed high-resolution transmission electron microscopy(HRTEM)shows numerous bonding configurations between the Al atoms and the graphene lattice,some of which are not theoretically ***,the produced AIG shows a CO_(2) capturability superior to those of other substitutionally doped graphenes.

关键词： aluminum-doped graphene single-solid precursor chemical vapor deposition carbon dioxide capture energy storage catalytic applications

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Dynamics of Primary Productivity and Oceanographic Parameters under Influence of the Amazon River Plume

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Open Journal of Ecology 2018年第11期8卷 590-606页

作者： Amanda Otsuka Fernando Feitosa Moacyr Araújo Dóris Veleda maria da Gloria Silva Cunha Nathalie Lefévre Felipe Gaspar Manuel Flores Montes Gislayne Borges Carlos Noriega Department of Oceanography Federal University of Pernambuco Recife Brazil IRD-LOCEAN Sorbonne Universités (Université Pierre et Marie Curie-CNRS-MNHN) Paris France

The watershed of the Amazon River discharges about 120,000 m3·s-1?of freshwater into the adjacent platform and oceanic region. The aim of this work was to analyze the distribution of oceanographic parameters, chlorophyll a and primary productivity under the influence of the Amazon River plume, during the period of greatest extension of the Amazon plume. Collections were carried out in September 14 in 16 stations including continental platform and oceanic region. It was possible to observe superficial currents along the coast in the northwest direction, but with less intensity and currents with greater speeds towards the east due to the North Brazil Current retroflexion at this time of the year, transporting the plume to the central Atlantic Ocean. The spatial influence of the plume was observed in the salinity, which ranged from 28 to 36.75, although the high precipitation in the region may also have contributed as a source of freshwater. However, the sampled region showed strong negative linear correlation of silicate, chlorophyll a and primary productivity with salinity. The primary productivity values ranged from 0.04 to 18.81 mg C m-3?day-1, whereas chlorophyll a concentrations ranged from 0.15 mg·m-3?to 1.83 mg·m-3, decreasing their values as they move away from the coast. The Amazon River plume can reach and influence the oceanographic and biological parameters in a large area of this oligotrophic region. However, the results also suggest that the export of material from the adjacent coastal region is another determinant of the region’s productivity.

关键词： Amazon River Plume Continental Shelf Oceanic Region Environmental Variables Phytoplankton Biomass Primary Productivity

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Graphene transfer methods: A review

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Nano Research 2021年第11期14卷 3756-3772页

作者： Sami Ullah Xiaoqin Yang Huy Q.Ta maria Hasan Alicja Bachmatiuk Klaudia Tokarska Barbara Trzebicka Lei Fu Mark H.Rummeli College of Energy Soochow Institute for Energy and Materials InnovationsKey Laboratory of Advanced Carbon Materials and Wearable Energy Technologies of Jiangsu ProvinceSoochow UniversitySuzhou215006China School of Energy and Power Engineering Xi’an Jiaotong UniversityXi’an710049China Institute for Complex Materials IFW Dresden20 Helmholtz StrasseDresden01069Germany NUTECH School of Applied Sciences&Humanities and Computer Engineering DepartmentNational University of TechnologyIslamabad42000Pakistan Centre of Polymer and Carbon Materials Polish Academy of SciencesM.Curie-Sklodowskiej 34Zabrze41-819Poland College of Chemistry and Molecular Science Wuhan UniversityWuhan430072China Institute of Environmental Technology VŠB-Technical University of Ostrava17.listopadu 15Ostrava70833Czech Republic

Graphene is a material with unique properties that can be exploited in electronics, catalysis, energy, and bio-related fields. Although, for maximal utilization of this material, high-quality graphene is required at both the growth process and after transfer of the graphene film to the application-compatible substrate. Chemical vapor deposition (CVD) is an important method for growing high-quality graphene on non-technological substrates (as, metal substrates, e.g., copper foil). Thus, there are also considerable efforts toward the efficient and non-damaging transfer of quality of graphene on to technologically relevant materials and systems. In this review article, a range of graphene current transfer techniques are reviewed from the standpoint of their impact on contamination control and structural integrity preservation of the as-produced graphene. In addition, their scalability, cost- and time-effectiveness are discussed. We summarize with a perspective on the transfer challenges, alternative options and future developments toward graphene technology.

关键词： high-quality transfer application-compatible substrate graphene technology

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Determination of double-and single-stranded DNA breaks in bovine sperm is predictive of their fertilizing capacity

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Journal of Animal Science and Biotechnology 2023年第1期14卷 134-151页

作者： Jordi Ribas‑Maynou Ariadna Delgado‑Bermúdez Yentel Mateo‑Otero Estel Vinolas Carlos O.Hidalgo WSteven Ward Marc Yeste Biotechnology of Animal and Human Reproduction(TechnoSperm) Faculty of SciencesInstitute of Food and Agricultural TechnologyUniversity of GironaC/Maria Aurèlia Campany69ES‑17003 GironaSpain. Unit of Cell Biology Department of BiologyFaculty of SciencesUniversity of GironaES‑17003 GironaSpain. Institute for Biogenesis Research Department of AnatomyBiochemistry&PhysiologyJohn A.Burns School of MedicineUniversity of Hawaii at ManoaHonoluluHI 96822USA. Department of Animal Selection and Reproduction The Regional Agri-Food Research and Development Service of Asturias(SERIDA)ES‑33394 GijónSpain. Catalan Institution for Research and Advanced Studies(ICREA) ES‑08010 BarcelonaSpain.

Background:The analysis of chromatin integrity has become an important determinant of sperm *** frozenthawed bovine sperm,neither the sequence of post-thaw injury events nor the dynamics of different types of sperm DNA breaks are well *** aim of the present work was to describe such sperm degradation aftermath focusing on DNA damage dynamics,and to assess if this parameter can predict pregnancy rates in ***:A total of 75 cryopreserved ejaculates from 25 Holstein bulls were evaluated at two post-thawing periods(0-2 h and 2-4 h),analyzing global and double-stranded DNA damage through alkaline and neutral Comet assays,chromatin deprotamination and decondensation,sperm motility,viability,acrosomal status,and intracellular levels of total ROS,superoxides and *** of 59,605 females was conducted using sperm from the same bulls,thus obtaining the non-return to estrus rates after 90 d(NRR).Results showed an increased rate of double-stranded breaks in the first period(0-2 h:1.29±1.01%/h vs.2-4 h:0.13±1.37%/h;P0.05).Finally,whereas no correlations between bull fertility and any dynamic parameter were found,there were correlations between the NRR and the basal percentage of highly-damaged sperm assessed with the alkaline Comet(Rs=-0.563,P=0.003),between NRR and basal progressive motility(Rs=0.511,P=0.009),and between NRR and sperm with high ROS at 4 h post-thaw(Rs=0.564,P=0.003).Conclusion:The statistically significant correlations found between intracellular ROS,sperm viability,sperm motility,DNA damage

关键词： Cattle Chromatin Comet test DNA damage Fertility Sperm Sperm quality

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Towards the integration of nanoemitters by direct laser writing on optical glass waveguides

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Photonics Research 2020年第9期8卷 1541-1550页

作者： XIAOLUN XU AURÉLIE BROUSSIER TIZIANA RITACCO MACKRINE NAHRA FABIEN GEOFFRAY ALI ISSA SAFI JRADI RENAUD BACHELOT CHRISTOPHE COUTEAU AND SYLVAIN BLAIZE Light nanomaterialsnanotechnologies(L2n)ERL 7004CNRS.University of Technology of Troyes12 rue Marie Curie10004 Troyes CedexFrance Teem Photonics 61 Chemin du Vieux Chêne38246 MeylanFrance Key Laboratory of Advanced Display and System Application Ministry of EducationSchool of Mechatronic Engineering and AutomationShanghai UniversityShanghai 200072China Sino-European School of Technology Shanghai UniversityShanghai 200044China

A major challenge towards nanophotonics is the integration of nanoemitters on optical *** the optical properties of nanoemitters with the benefits of integration and scalability of integrated optics is still a major issue to *** this work,we demonstrate the integration of nanoemitters positioned in a controlled manner onto a substrate and onto an optical ion-exchanged glass waveguide via direct laser writing based on twophoton *** nanoemitters are colloidal CdSe/ZnS quantum dots(QDs)embedded in polymeric *** varying the laser parameters during the patterning process,we make size-controlled QDpolymer nanostructures that were systematically characterized using optical and structural *** as small as 17 nm in height were *** well-controlled QD-polymer nanostructure systems were then successfully integrated onto a new photonic platform for nanophotonics made of an ion-exchanged *** show that our QDs maintain their light emitting quality after integration as verified by photoluminescence(PL)***,QD emission coupled to our waveguides is detected through a home-built fiber-edge coupling PL measurement *** results show the potential for future integration of nanoemitters onto complex photonic chips.

关键词： emitter optical waveguides

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Exploring the limits of complexity: A survey of empirical studies on graph visualisation

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Visual Informatics 2018年第4期2卷 264-282页

作者： Vahan Yoghourdjian Daniel Archambault Stephan Diehl Tim Dwyer Karsten Klein Helen C.Purchase Hsiang-Yun Wu Monash university Australia Swansea university United Kingdom university of Trier Germany university of Konstanz Germany university of Glasgow United Kingdom university of TU Wien Austria

For decades,researchers in information visualisation and graph drawing have focused on developing techniques for the layout and display of very large and complex *** involving human participants have also explored the readability of different styles of layout and representations for such *** both bodies of literature,networks are frequently referred to as being‘large’or‘complex’,yet these terms are *** a human-centred,experiment point-of-view,what constitutes‘large’(for example)depends on several factors,such as data complexity,visual complexity,and the technology *** this paper,we survey the literature on human-centred experiments to understand how,in practice,different features and characteristics of node–link diagrams affect visual complexity.

关键词： Graph visualisation Network visualisation node-link diagrams Cognitive scalability Evaluations Empirical studies

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MIXED VARIATIONAL INEQUALITIES DRIVEN BY FRACTIONAL EVOLUTIONARY EQUATIONS

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Acta Mathematica Scientia 2019年第2期39卷 461-468页

作者： Stanislaw MIGóRSKI Shengda ZENG College of Sciences Beibu Gulf University Jagiellonian university in Krakow Faculty of Mathematics and Computer Science Jagiellonian University in Krakow

The goal of the present paper is to investigate an abstract system, called fractional differential variational inequality, which consists of a mixed variational inequality combined with a fractional evolution equation in the framework of Banach spaces. Using discrete approximation approach, an existence theorem of solutions for the inequality is established under some suitable assumptions.

关键词： fractional differential variational inequality C_0-semigroup Minty mixed variational inequality existence mild solutions

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Towards a Service-Oriented Architecture for a Mobile Assistive System with Real-time Environmental Sensing

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Tsinghua Science and Technology 2016年第6期21卷 581-597页

作者： Darpan Triboan Liming Chen Feng Chen Zumin Wang the Context Intelligence and Interaction Research Group (CIIRG) De Montfort University the Department of Information Engineering Dalian University

With the growing aging population, age-related diseases have increased considerably over the *** response to these, Ambient Assistive Living（AAL） systems are being developed and are continually evolving to enrich and support independent living. While most researchers investigate robust Activity Recognition（AR）techniques, this paper focuses on some of the architectural challenges of the AAL systems. This work proposes a system architecture that fuses varying software design patterns and integrates readily available hardware devices to create Wireless Sensor Networks（WSNs） for real-time applications. The system architecture brings together the Service-Oriented Architecture（SOA）, semantic web technologies, and other methods to address some of the shortcomings of the preceding system implementations using off-the-shelf and open source components. In order to validate the proposed architecture, a prototype is developed and tested positively to recognize basic user activities in real time. The system provides a base that can be further extended in many areas of AAL systems,including composite AR.

关键词： Activities of Daily Living (ADL) Service-Oriented Architecture (SOA) semantic web ontology modeling Web Ontology Language (OWL) Activity Recognition (AR) Smart Homes (SH) Wireless Sensor Networks (WSNs)

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