检索结果-南通市图书馆

Differential Roles of Arabidopsis Dynamin-Related Proteins DRP3A,DRP3B,and DRP5B in Organelle Division

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative plant Biology 2012年第11期54卷 921-931页

作者： Kyaw Aung msu-doe plant research laboratory and plant Biology Department Michigan State UniversityEast LansingMI 48824USA

Dynaminrelated proteins （DRPs） are key components of the organelle division machineries, functioning as molecular scissors during the fission process. In Arabidopsis, DRP3A and DRP3B are shared by peroxisomal and mitochondrial division, whereas the structurallydistinct DRP5B （ARC5） protein is involved in the division of chloroplasts and peroxisomes. Here, we further investigated the roles of DRP3A, DRP3B, and DRP5B in organelle division and plant development. Despite DRP5B＇s lack of stable association with mitochondria, drp5B mutants show defects in mitochondrial division.

关键词： Arabidopsis dynamin-related protein mitochondria.

Arabidopsis RING Peroxins are E3 Ubiquitin Ligases that Interact with Two Homologous Ubiquitin Receptor Proteins

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative plant Biology 2013年第1期55卷 108-120页

作者： Navneet Kaur Qingzhen Zhao Qi Xie Jianping Hu msu-doe plant research laboratory Michigan State University Institute of Genetics and Developmental Biology the Chinese Academy of Sciences

Peroxisomes are essential eukaryotic organelles that mediate various metabolic processes. Peroxisome import depends on a group of peroxisome biogenesis factors called peroxins, many of which are evolutionarily conserved. PEX2, PEX10, and PEX12 are three RING-finger-domain-containing integral membrane peroxins crucial for protein import. In yeast （Saccharomyces cerevisae）, RING peroxins act as E3 ligases, facilitating the recycling of the peroxisome import receptor protein PEX5 through ubiquitination. In plants, RING peroxins are essential to plant vitality. To elucidate the mode of action of the plant RING peroxins, we employed in vitro assays to show that the Arabidopsis RING peroxins also have E3 ligase activities. We also identified a PEX2-interacting protein, DSK2b, which is a member of the ubiquitin receptor family known to function as shuttle factors ferrying polyubiquitinated substrates to the proteasome for degradation. DSK2b and its tandem duplicate DSK2a are localized in the cytosol and the nucleus, and both interact with the RING domain of PEX2 and PEX12. DSK2 artificial microRNA lines did not display obvious defects in plant growth or peroxisomal processes, indicating functional redundancies among Arabidopsis ubiquitin receptor proteins. Our results suggest that Arabidopsis RING peroxins can function as E3 ligases and act together with the ubiquitin receptor protein DSK2 in the peroxisomal membrane-associated protein degradation system.

关键词： Arabidopsis RING domain proteins peroxisomes ubiquitin receptor.

FISSION 1A and FISSION 1 B Proteins Mediate the Fission of Peroxisomes and Mitochondria in Arabidopsis

在线全文

学校读者我要写书评

暂无评论

Molecular plant 2008年第6期1卷 1036-1047页

作者： Xin-Chun Zhang Jian-Ping Hu msu-doe plant research laboratory Michigan State University East Lansing MI 48824 USA plant Biology Department Michigan State University East Lansing MI 48824 USA

Peroxisomes and mitochondria are metabolically diverse organelles that act in concert in a number of pathways in eukaryotes, including photorespiration and lipid mobilization in plants. The division machineries of these two types of organelles also share several components such as dynamin-related proteins （DRPs） and their organelle anchor, the FISSION1 （FIS1） protein. In Arabidopsis, members of the DRP3 and FIS1 small protein families, namely DRP3A, DRP3B, FISIA, and FISIB, are each dual-targeted to peroxisomes and mitochondria and are required for the division of both organelles; DRP3A and DRP3B are partially redundant in function. To further determine the contribution of FISIA and FISIB to the division of peroxisomes and mitochondria, we analyzed plants overexpressing FISIA or FISIB and mutants in which the functions of both proteins were disrupted. Domains in FISIA and FISIB required for peroxisomal targeting were also dissected. Our results demonstrate that FISIA and FISIB play rate-limiting and partially overlapping roles in promoting the fission of peroxisomes and mitochondria. Furthermore, although the C-terminus of FIS1 is both necessary and sufficient for targeting to peroxisomes, the role of the short C-terminal segment adjacent to the transmembrane domain may differ among diverse species in peroxisomal targeting.

关键词： peroxisomal and mitochondrial fission Arabidopsis FIS1 protein.

Arabidopsis Forkhead-Associated Domain Protein 3 negatively regulates peroxisome division

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative plant Biology 2017年第7期59卷 454-458页

作者： Mintu Desai Ronghui Pan Jianping Hu msu-doe plant research laboratory Michigan State University Thermo Fisher Scientific plant Biology Department Michigan State University

Peroxisomes are ubiquitous and dynamic eukaryotic organelles capable of altering their abun- dance in response to environmental and developmental cues, yet the regulatory mechanism of plant peroxisome division/proliferation is unclear. To identify transcriptional regulators of the peroxisome division factor gene PEX 11b, we performed a nuclear pull-clown experiment and identified Arabidopsis Forkhead- Associated Domain Protein 3 （FHA3） as a novel protein that binds to the promoter of PEX 11b. Our data supported the conclusion that, in contrast to the previously identified HY5 HOMOLOG （HYH） protein that promotes the transcription of PEX 11b, FHA3 is a negative regulator of PEX 11b expression and peroxisome division.

关键词： FHA as that of gene Figure PEX Arabidopsis Forkhead-Associated Domain Protein 3 negatively regulates peroxisome division

Electrocatalytic cleavage of a lignin β-O-4 model compound and coupling with nitrogen-containing aromatics using Prussian blue analogue-derived nickel–cobalt spinel

在线全文

学校读者我要写书评

暂无评论

Journal of Energy Chemistry 2025年第1期100卷 628-636页

作者： Yi Qi Xueying Chen Bowen Liu Xuliang Lin Xueqing Qiu Yanlin Qin Guangdong Provincial Key laboratory of plant Resources Biorefinery School of Chemical Engineering and Light Industry Guangdong University of Technology Guangdong Provincial laboratory of Chemistry and Fine Chemical Engineering Jieyang Center Guangdong Basic research Center of Excellence for Ecological Security and Green Development Guangdong University of Technology

Electrochemical conversion of lignin for the production of high-value heterocyclic aromatic compounds has great *** demonstrate the targeted synthesis and cation modulation of NiCo2O4spinel nanoboxes,synthesized via cation exchange and calcination *** catalysts exhibit excellent efficacy in the electrocatalytic conversion of lignin model compounds,specifically 2-phenoxy-1-phenylethanol,into nitrogen-containing aromatics,achieving high conversion rates and *** catalysts were synthesized via a cation exchange and calcination oxidation process,using Prussian blue nanocubes as *** porous architecture and polymetallic composition of the NiCo2O4spinel demonstrated superior performance in electrocatalytic oxidative coupling,achieving a 99.2 wt%conversion rate of the 2-phenoxy-1-phenylethanol with selectivities of 37.5 wt%for quinoline derivatives and 31.5 wt%for *** innovations include the development of a sustainable one-pot synthesis method for quinoline derivatives,the elucidation of a multistage reaction pathway involving CAO bond cleavage,hydroxyaldol condensation,and CAN bond formation,and a deeper mechanistic understanding derived from DFT *** work establishes a new strategy for lignin valorization,offering a sustainable route to produce high-value nitrogen-containing aromatics from renewable biomass under mild conditions,without the need for additional reagents.

关键词： Spinel Biomass valorization Electrocatalysis Lignin oxidation Coupling

The Integrity of the plant Golgi Apparatus Depends on Cell Growth-Controlled Activity of GNL1

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Molecular plant 2013年第3期6卷 905-915页

作者： Wenyan Du Kentaro Tamura Giovanni Stefano Federica Brandizzi msu-doe plant research lab Michigan State University East Lansing MI 48824 USA Department of plant Biology Michigan State University East Lansing MI 48824 USA Department of Botany Graduate School of Science Kyoto University Sakyo-ku Kyoto 606-8502 Japan

Membrane traffic and organelle integrity in the plant secretory pathway depend on ARF-GTPases, which are activated by guanine-nucleotide exchange factors （ARF-GEFs）. While maintenance of conserved roles, evolution of unique functions as well as tissue-specific roles have been shown for a handful of plant ARF-GEFs, a fundamental yet unanswered question concerns the extent to which their function overlaps during cell growth. To address this, we have characterized pao, a novel allele of GNOM-like 1 （GNL1）, a brefeldin A （BFA）-insensitive ARF-GEF, isolated through a confocal microscopy-based forward genetics screen of the Golgi in Arabidopsis thaliana. Specifically, we have analyzed the dependence of the integrity of trafficking routes and secretory organelles on GNL1 availability during expansion stages of cotyledon epidermal cells, an exquisite model system for vegetative cell growth analyses in intact tissues. We show that Golgi traffic is influenced largely by GNL1 availability at early stages of cotyledon cell expansion but by BFA- sensitive GEFs when cell growth terminates. These data reveal an unanticipated level of complexity in the biology of GNL1 by showing that its cellular roles are correlated with cell growth. These results also indicate that the cell growth stage is an important element weighting into functional analyses of the cellular roles of ARF-GEFs.

关键词： ARF-GEFs Golgi Arabidopsis GNL1 brefeldin A.

Maximizing biomass utilization: An integrated strategy for coproducing multiple chemicals

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Energy Chemistry 2025年第1期100卷 180-191页

作者： Byeongchan Ahn Sua Jo Jonggeol Na J.Jay Liu Young-Ju Kim Wangyun Won Department of Chemical and Biological Engineering Korea University Department of Chemical Engineering (Integrated process) Kyung Hee University Department of Chemical Engineering and Materials Science Ewha Womans University Department of Chemical Engineering Pukyong National University Resources Engineering plant research Department Korea Institute of Geoscience and Mineral Resources

Lignocellulosic biomass is one of the viable solutions to alleviate the global warming. However, the limited utilization of biomass majorly focused on cellulose and hemicellulose restricts the economic and environmental feasibilities. To cope with this issue, we proposed an integrated process of co-producing 1,6-hexanediol（1,6-HDO） with tetrahydrofuran and adipic acid from biomass, referred to as Strategy A. To compare the impacts of lignin upgrading and feedstock, Strategy B, which co-produces tetrahydrofuran alone, and Strategy C, which is the traditional route to produce 1,6-HDO from fossil fuels, were used. Heat networks are also designed to reduce operating costs and indirect carbon emissions due to energy consumption, saving 87% and 83% of the heat and cooling requirements, respectively, in Strategy A. The market competitiveness of Strategy A was evaluated by determining the minimum selling price through techno-economic analysis, and sustainability was thoroughly investigated by quantifying the environmental impacts through both midpoint and endpoint life-cycle assessments（LCAs）.Strategy A was found to be the most favorable both economically（USRDSCHARDOLLAR3,402/ton） and environmentally（-26.9 kg CO2eq.）. This indicates that lignin valorization is not only economically but also environmentally preferred. Finally, changes in economic and environmental feasibilities depending on economic, process, and environmental parameters were investigated using sensitivity and uncertainty analyses. The results of these analyses provide valuable insight into bio-based chemical production.

关键词： Biorefinery Carbon mitigation Climate change Global warming Lignin utilization Bioeconomy

Cold-Induced CBF–PIF3 Interaction Enhances Freezing Tolerance by Stabilizing the phyB Thermosensor in Arabidopsis

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Molecular plant 2020年第6期13卷 894-906页

作者： Bochen Jiang Yiting Shi Yue Peng Yuxin Jia Yan Yan Xiaojing Dong Hui Li Jie Dong Jigang Li Zhizhong Gong Michael F.Thomashow Shuhua Yang State Key laboratory of plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China Department of Molecular Cellular and Developmental BiologyYale UniversityNew HavenCT 06520USA msu-doe plant research lab Michigan State UniversityEast LansingMI 48824USA

Growth inhibition and cold-acclimation strategies help plants withstand cold stress,which adversely affects growth and *** B(phyB)regulates plant growth through perceiving both light and ambient temperature ***,the mechanism by which phyB mediates the plant response to cold stress remains ***,we show that the key transcription factors mediating cold acclimation,C-REPEAT BINDING FACTORs(CBFs),interact with PHYTOCHROME-INTERACTING FACTOR 3(PIF3)under cold stress,thus attenuating the mutually assured destruction of PIF3–***-stabilized phyB acts downstream of CBFs to positively regulate freezing tolerance by modulating the expression of stress-responsive and growth-related *** with this,phyB mutants exhibited a freezing-sensitive phenotype,whereas phyB-overexpression transgenic plants displayed enhanced freezing *** analysis showed that the PIF1,PIF4,and PIF5 proteins,all of which negatively regulate plant freezing tolerance,were destabilized by cold stress in a phytochrome-dependent ***,our study reveals that CBFs–PIF3–phyB serves as an important regulatory module for modulating plant response to cold stress.

关键词： CBFs PIFs phyB freezing tolerance Arabidopsis

Arabidopsis CAMTA Transcription Factors Regulate Pipecolic Acid Biosynthesis and Priming of Immunity Genes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular plant 2020年第1期13卷 157-168页

作者： Yongsig Kim Sarah J.Gilmour Lumen Chao Sunchung Park Michael F.Thomashow msu-doe plant research laboratory Michigan State UniversityEast LansingMl 48824USA msu plant Resilience Institute Michigan State UniversityEast LansingMl 48824USA Department of plant Soil and Microbial SciencesMichigan State UniversityEast LansingMl 48824USA Present address:U.S.Department of Agriculture Agricultural Research Service1636 East Alisal St.SalinasCA 93905USA

The Arabidopsis thaliana Calmodulin-binding Transcription Activator(CAMTA)transcription factors CAMTA1,CAMTA2,and CAMTA3(CAMTA123)serve as master regulators of salicylic acid(SA)-mediated immunity,repressing the biosynthesis of SA in healthy ***,we show that CAMTA123 also repress the biosynthesis of pipecolic acid(Pip)in healthy *** of CAMTA123 function resulted in the induction of AGD2-like defense response protein 1(ALD1),which encodes an enzyme involved in Pip *** of ALD1 resulted in the accumulation of high levels of Pip,which brought about increased levels of the SA receptor protein NPR1 without induction of NPR1 expression or requirement for an increase in SA ***-mediated induction of ALD1 and genes regulating the biosynthesis of SA—CBP60g,SARD1,PAD4,and EDS1—was largely dependent on ***,Pip-mediated increase in NPR1 protein levels was associated with priming of Pip and SA biosynthesis genes to induction by low levels of *** together,our findings expand the role for CAMTA123 in regulating key immunity genes and suggest a working model whereby loss of CAMTA123 repression leads to the induction of plant defense genes and initiation of SAR.

关键词： Arabidopsis thaliana pipecolic acid salicylic acid plant immunity priming CAMTA transcription factors