检索结果-南通市图书馆

An improved installation of 2-hydroxy-4-methoxybenzyl(iHmb)method for chemical protein synthesis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2024年第5期35卷 145-150页

作者： Ying Li Long-Jie Wang Yong-Kang Zhou Jun Liang Bin Xiao Ji-Shen Zheng The First Affiliated Hospital of USTC MOE Key Laboratory for Membraneless Organelles and Cellular DynamicsDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001China Department of Chemistry University of Science and Technology of ChinaHefei 230027China

The 2-hydroxy-4-methoxybenzyl(Hmb)backbone modification can prevent amide bond-mediated sidereactions(e.g.,aspartimide formation,peptide aggregation)by installing the removable Hmb group into a peptide bond,thus improving the synthesis of long and challenging peptides and ***,its use is largely precluded by the limited Hmb’s installation *** this report,an improved installation of Hmb(iHmb)method was developed to achieve the flexible installation and the convenient removal of *** iHmb method involves two critical steps:(1)oxidative diazotization of the readily installed 2-hydroxy-4-methoxy-5-amino-benzyl(Hmab)to give 2-hydroxy-4-methoxy-5-diazonium-benzyl(Hmdab)by combining soamyl nitrite(IAN)/HBF_(4),and(2)reductive elimination of Hmdab to give the desired Hmb by 1,2-ethanedithiol(EDT).The iHmb method enables the installation of Hmb at any primary amino acid including the highly sterically hindered amino acids(e.g.,valine and isoleucine).The practicality and utility of the iHmb method was demonstrated by one-shot solid-phase synthesis of a challenging aspartimide-prone peptide,the mirror-image version of a hydrophobic peptide and a long-chain peptide up to ***,the iHmb method can be utilized to facilitate chemical protein ligation,as exemplified by the synthesis of the single-spanning membrane protein *** iHmb method expands the toolkit for peptide synthesis and ligation and facilitates the preparation of peptides/proteins.

关键词： Chemical protein synthesis Solid-phase peptide synthesis Removable backbone modification 2-Hydroxy-4-methoxybenzyl(Hmb)group Aspartimide-prone peptides Difficult peptides Membrane proteins

Organization of microtubule plus-end dynamics by phase separation in mitosis

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Journal of Molecular Cell Biology 2024年第2期16卷 24-35页

作者： Fengrui Yang Mingrui Ding Xiaoyu Song Fang Chen Tongtong Yang Chunyue Wang Chengcheng Hu Qing Hu Yihan Yao Shihao Du Phil YYao Peng Xia Gregory Adams Jr Chuanhai Fu Shengqi Xiang Dan Liu Zhikai Wang Kai Yuan Xing Liu moe key laboratory for membraneless organelles&cellular dynamics Hefei National Research Center for Cross-disciplinary SciencesCenter for Advanced Interdisciplinary Science and Biomedicine of IHMDivision of Life Sciences and MedicineUniversity of Science&Technology of ChinaHefei 230027China Anhui key laboratory for cellular dynamics and Chemical Biology University of Science&Technology of ChinaHefei 230027China Keck Center for Organoids Plasticity Morehouse School of MedicineAtlantaGA 30310USA Hunan key laboratory of Molecular Precision Medicine Central South UniversityChangsha 410083China

In eukaryotes,microtubule polymers are essential for cellular plasticity and fate ***-binding(EB)proteins serve as scaffolds for orchestrating microtubule polymer dynamics and are essential for cellular dynamics and chromosome segregation in ***,we show that EB1 forms molecular condensates with TIP150 and MCAK through liquid–liquid phase separation to compartmentalize the kinetochore–microtubule plus-end machinery,ensuring accurate kinetochore–microtubule interactions during chromosome segregation in *** of EB1–TIP150 polymer formation by a competing peptide prevents phase separation of the EB1-mediated complex and chromosome alignment at the metaphase equator in both cultured cells and Drosophila ***220 of EB1 is dynamically acetylated by p300/CBP-associated factor in early mitosis,and persistent acetylation at Lys220 attenuates phase separation of the EB1-mediated complex,dissolves droplets in vitro,and harnesses accurate chromosome *** data suggest a novel framework for understanding the organization and regulation of eukaryotic spindle for accurate chromosome segregation in mitosis.

关键词： phase separation mitosis microtubule dynamics EB1 acetylation

CSPP1 stabilizes microtubules by capping both plus and minus ends

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Molecular Cell Biology 2024年第2期16卷 36-46页

作者： Zhikai Wang Wenwen Wang Shuaiyu Liu Fengrui Yang Xu Liu Shasha Hua Lĳuan Zhu Aoqing Xu Donald LHill Dongmei Wang Kai Jiang Jennifer Lippincott-Schwartz Xing Liu Xuebiao Yao moe key laboratory for membraneless organelles and cellular dynamics Center for Advanced Interdisciplinary Science and Biomedicine of IHMUniversity of Science and Technology of China School of Life SciencesHefei 230027China Anhui key laboratory for cellular dynamics and Chemical Biology Hefei National Research Center for Interdisciplinary Sciences at the MicroscaleHefei 230027China Frontier Science Center for Immunology and Metabolism Medical Research InstituteWuhan UniversityWuhan 430071China Comprehensive Cancer Center University of AlabamaBirminghamAL 35233USA Janelia Research Campus Howard Hughes Medical InstituteAshburnVA 20147USA

Although the dynamic instability of microtubules(MTs)is fundamental to many cellular functions,quiescent MTs with unattached free distal ends are commonly present and play important roles in various events to power cellular ***,how these free MT tips are stabilized remains poorly ***,we report that centrosome and spindle pole protein 1(CSPP1)caps and stabilizes both plus and minus ends of static ***-time imaging of laser-ablated MTs in live cells showed deposition of CSPP1 at the newly generated MT ends,whose dynamic instability was concomitantly ***,MT ends in CSPP1-overexpressing cells were hyper-stabilized,while those in CSPP1-depleted cells were much more *** CSPP1-elicited stabilization of MTs was demonstrated to be achieved by suppressing intrinsic MT catastrophe and restricting ***,CSPP1-bound MTs were resistant to mitotic centromere-associated kinesin-mediated *** findings delineate a previously uncharacterized CSPP1 activity that integrates MT end capping to orchestrate quiescent MTs.

关键词： CSPP1 microtubule plus end minus end capping

The innovative safe herbicide dienediamine saves paraquat

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2024年第1期17卷 11-12页

作者： Jin-Qiu Xia Ping Xu Cheng-Bin Xiang Division of Life Sciences and Medicine Division of Molecular&Cell BiophysicsHefei National Science Center for Physical Sciences at the MicroscaleMOE Key Laboratory for Membraneless Organelles and Cellular DynamicsUniversity of Science and Technology of ChinaThe Innovation Academy of Seed DesignChinese Academy of SciencesHefeiAnhui Province 230027China Shanghai key laboratory of Plant Functional Genomics and Resources Shanghai Chenshan Botanical GardenShanghai Chenshan Plant Science Research CenterCAS Center for Excellence in Molecular Plant SciencesShanghai 201602China

Throughout the history of agriculture,the challenge of weed management has endured,necessitating significant investments to facilitate ideal crop growth and maximize *** for weed control mainly involve manual and chem... 详细信息

关键词： approach. manual chemical

Biomolecular condensates and disease pathogenesis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2024年第9期67卷 1792-1832页

作者： Ke Ruan Ge Bai Yanshan Fang Dan Li Tingting Li Xingguo Liu Boxun Lu Qing Lu Zhou Songyang Shuguo Sun Zheng Wang Xin Zhang Wen Zhou Hong Zhang The First Affiliated Hospital&School of Life Sciences Ministry of Education Key Laboratory for Membrane-less Organelles&Cellular DynamicsHefei National Research Center for Interdisciplinary Sciences at the MicroscaleCenter for Advanced Interdisciplinary Science and Biomedicine of IHMDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230027China Nanhu Brain-computer Interface Institute Hangzhou 311100China Department of Neurology of Second Affiliated Hospital and School of Brain Science and Brain Medicine Zhejiang University School of MedicineHangzhou 310058China Interdisciplinary Research Center on Biology and Chemistry Shanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai 201210China University of Chinese Academy of Sciences Beijing 100049China Bio-X Institutes Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders(Ministry of Education)Shanghai Jiao Tong UniversityShanghai 200030China Department of Biomedical Informatics School of Basic Medical SciencesPeking University Health Science CenterBeijing 100191China CAS key laboratory of Regenerative Biology Joint School of Life SciencesGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China Joint School of Life Sciences Guangzhou Medical UniversityGuangzhou 510000China Neurology Department at Huashan Hospital State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain ScienceInstitutes of Brain ScienceSchool of Life SciencesFudan UniversityShanghai 200433 China key laboratory for the Genetics of Developmental and Neuropsychiatric Disorders Ministry of EducationBio-X InstitutesShanghai Jiao Tong UniversityShanghai 200030China State key laboratory of Biocontrol MOE Key Laboratory of Gene Function and Regulation and Guangzhou Key Laboratory of Healthy Aging ResearchSchool of Life SciencesSun Yat-sen Memorial HospitalSun Yat-sen UniversityGuangzhou 510275China Department of Human Anatomy Hist

Biomolecular condensates or membraneless organelles(MLOs)formed by liquid-liquid phase separation(LLPS)divide intracellular spaces into discrete compartments for specific *** of LLPS or aberrant phase transition that disturbs the formation or material states of MLOs is closely correlated with neurodegeneration,tumorigenesis,and many other pathological ***,we summarize the recent progress in development of methods to monitor phase separation and we discuss the biogenesis and function of MLOs formed through phase *** then present emerging proof-of-concept examples regarding the disruption of phase separation homeostasis in a diverse array of clinical conditions including neurodegenerative disorders,hearing loss,cancers,and immunological ***,we describe the emerging discovery of chemical modulators of phase separation.

关键词： liquid-liquid phase separation biomolecular condensate membraneless organelle

Modeling cellular polarity,plasticity,and disease disparity in 4D

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Journal of Molecular Cell Biology 2020年第8期12卷 559-561页

作者： Xuebiao Yao moe key laboratory for membraneless organelles&cellular dynamics and CAS Center for Excellence in Molecular Cell Science University of Science and Technology of China School of Life ScienceHefei 230027China

Most cell types are polarized with distinct structural orientations or protein localization patterns that allow cells to perform context-dependent functions in space and time in response to physiological regulation(Forte and Yao,1996).Epithelial cell polarity is characterized by cells with apical and basolateral membrane domains,and the establishment and maintenance of cell polarity are crucial during development to specify cell fate and functions,e.g.

关键词： polarity Forte plasticity

Orchestration of cell plasticity by phase separation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Molecular Cell Biology 2021年第4期13卷 237-238页

作者： Xuebiao Yao moe key laboratory for membraneless organelles&cellular dynamics and CAS Center for Excellence in Molecular Cell Science School of Life SciencesUniversity of Science and Technology of ChinaHefei 230027China

Liquid-liquid phase separation(LLPS)of biomolecules drives the formation of cellular compartments,which determines cell plasticity and fate *** evidence indicates that a large number of membraneless organelles(MLOs),including nucleoli,Cajal bodies,P-bodies,and stress granules,exist as liquid droplets within the cell and orchestrate the cell plasticity control in response to the dynamics of extracellular cues.

关键词： separation plasticity phase

Mechanisms and regulation underlying membraneless organelle plasticity control

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Molecular Cell Biology 2021年第4期13卷 239-258页

作者： Hazrat Ismail Xu Liu Fengrui Yang Junying Li Ayesha Zahid Zhen Dou Xing Liu Xuebiao Yao moe key laboratory for membraneless organelles&cellular dynamics and CAS Center for Excellence in Molecular Cell Science Hefei National Laboratory for Physical Sciences at the MicroscaleUniversity of Science and Technology of ChinaHefei 230027China Keck Center for Organoids Plasticity Control AtlantaGA 30310USA Anhui key laboratory for cellular dynamics&Chemical Biology Hefei 230027China

Evolution has enabled living cells to adopt their structural and functional complexity by organizing intricate cellular compartments,such as membrane-bound and membraneless organelles(MLOs),for spatiotemporal catalysis of physiochemical reactions essential for cell plasticity *** evidence and view support the notion that MLOs are built by multivalent interactions of biomolecules via phase separation and transition *** healthy cells,dynamic chemical modifications regulate MLO plasticity,and reversible phase separation is essential for cell *** evidence revealed that aberrant phase separation results in numerous neurodegenerative disorders,cancer,and other *** this review,we provide molecular underpinnings on(i)mechanistic understanding of phase separation,(ii)unifying structural and mechanistic principles that underlie this phenomenon,(iii)various mechanisms that are used by cells for the regulation of phase separation,and(iv)emerging therapeutic and other applications.

关键词： liquid-liquid phase separation membraneless organelles biomolecular condensates intrinsically disordered proteins post-translational modifications

Phase separation drives decision making in cell division

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Phase separation drives decision making in cell division