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Journal of Genetics and Genomics 2008年第5期35卷 291-297页

作者： Dhanikachalam Velu Kangayam M. Ponnuvel Murugiah Muthulakshmi Randhir K. Sinha Syed M.H. Qadri biotechnology Laboratory Central Sericultural Germplasm Resources Centre Hosur-635109 Tamil Nadu India

Amplified inter simple sequence repeats （ISSR） markers were used to determine genetic relationships among mutant silkworm strains of Bombyx mori. Fifteen ISSR primers containing simple sequence repeat （SSR） motifs were used in this study. A total of 113 markers were produced among 20 mutant strains, of which 73.45% were found to be polymorphic. In selected mutant genetic stocks, the average number of observed allele was （1.7080 ± 0.4567）, effective alleles （1.5194 ± 0.3950） and genetic diversity （Ht）（0.2901 ± 0.0415）. The dendrogram produced using the unweighted pair group method with arithmetic means （UPGMA） and cluster analysis made using Nei＇s genetic distance resulted in the formation of one major group containing 6 groups separated 20 mutant silkworm strains. Therefore, ISSR amplification is a valuable method for determining the genetic variability among mutant silkworm strains. This efficient molecular marker would be useful for characterizing a considerable number of silkworm strains maintained at the germplasm center.

关键词： genetic diversity Bombyx mori mutant strains ISSR

Single carbon metabolism–A new paradigm for microbial bioprocesses?

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Synthetic and Systems biotechnology 2024年第2期9卷 322-329页

作者： Michael Baumschabl Özge Ata Diethard Mattanovich Austrian centre of Industrial biotechnology(ACIB) Vienna1190Austria University of Natural resources and Life Sciences Department of BiotechnologyInstitute of Microbiology and Microbial BiotechnologyViennaAustria

Increasing atmospheric carbon dioxide levels,a reduction of arable land area and the dependence of first and second generation biotechnology feedstocks on agricultural products,call for alternative,sustainable feedstock sources for industrial *** direct use of CO_(2)or conversion of CO_(2)into other single carbon(C1)sources have great potential as they might help to reduce carbon emissions and do not compete with agricultural land *** we discuss the microbial use of C1 carbon sources,their potential applications in biotechnology,and challenges towards sustainable C1-based industrial biotechnology *** focus on methanol,formic acid,methane,syngas,and CO_(2)as feedstocks for bioprocesses,their assimilation pathways,current and emerging applications,and limitations of their *** mini-review is intended as a first introduction for researchers who are new to the field of C1 biotechnology.

关键词： Sustainability BioeconomyCarbon dioxide Methanol Formate Methane

In Vitro Evaluation of the Antimethanogenic Potency and Effects on Fermentation of Individual and Combinations of Marine macroalgae

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American Journal of Plant Sciences 2016年第14期7卷 2038-2054页

作者： Robert D. Kinley Matthew J. Vucko Lorenna Machado Nigel W. Tomkins CSIRO Agriculture Australian Tropical Science & Innovation Precinct James Cook University Townsville Australia macro—the centre for macroalgal resources and biotechnology College of Marine and Environmental Sciences James Cook University Townsville Australia

Contribution of ruminants to total greenhouse gas emissions in Australia is approximately 10% and likely to increase with demand for livestock products, thus an efficient method of mitigation must be implemented. The red marine macroalgae Asparagopsis taxiformis reduces enteric methane production by up to 99% in vitro. Other macroalgae with less potent antimethanogenic properties may complement inclusion of Asparagopsis in livestock feeds. Adoption of environmental based changes in livestock systems must provide benefits to producers if change in management is to be adopted. This study used 72 h in vitro fermentations with rumen inoculum to characterize and rank seven species of macroalgae at low inclusion that previously demonstrated some degree of antimethanogenesis at higher inclusion concentration. The seven were assessed at 5% inclusion (OM basis) and in combination with Asparagopsis to evaluate beneficial effects on fermentation. When tested individually, improvements in volatile fatty acids were generally observed, however, minimal effect on gas production and no clear justification for a ranking order were demonstrated. When tested in combination with Asparagopsis, the effects on fermentation were dominated by presence of Asparagopsis at 2% and no further benefits demonstrated. Therefore, Asparagopsis remains the only macroalga inducing near elimination of methane in vitro and benefit of combinations with other macroalgae evaluated in this study was not demonstrated. However, combination with high protein macroalgae is proposed to provide productivity enhancement during seasonal lows in grass quality and thus reduce methane emissions intensity providing a stronger conduit for environmental responsibility while increasing productivity.

关键词： Asparagopsis Methane Rumen Seaweed Algae

Genetic Diversity in ex-situ Conserved Lens culinaris for Botanical Descriptors, Biochemical and Molecular Markers and Identification of Landraces from Indigenous Genetic resources of Pakistan

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Journal of Integrative Plant Biology 2008年第4期50卷 484-490页

作者： Tayyaba Sultana Abdul Ghafoor Government College University Faisalabad PakistanPlant Genetic Resources Program Institute of Agri Biotechnology and Genetic Resources National Agricultural Research Centre Islamabad Pakistan Government College University Faisalabad Pakistan

Lentil, one of the oldest legumes was investigated for diversity based on botanical descriptors, total seed proteins, isozymes and random amplified polymorphic DNA （RAPD） markers. About one fourth of accessions were heterogeneous for botanical descriptors and a seed protein profile. The germplasm collected from the province of Baluchiatan revealed the prevalence of indigenous landraces as high diversity was observed for all of the techniques. Diversity explored through various techniques revealed validity irrespective of the sample size or geographic pattem, RAPD being the best choice for investigating both inter- and intra-acceaaion variation in lentil. Although all of the techniques were able to resolve genetic diversity in lentil, isozymea and seed proteins gave low levels of genetic diversity, suggesting that more investigation into isozymea of specific proteins is required. RAPD is the best option for determining inter- and intra-acceaaion variation, and will be required to extend germplaama and primers to continue the study of botanical descriptors.

关键词： DNA marker genetic resource lentil sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Assessment of Variation in Morpho-Physiological Traits and Genetic Diversity in Relation to Submergence Tolerance of Five Indigenous Lowland Rice Landraces

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Rice science 2020年第1期27卷 32-43页

作者： Jijnasa BARIK Vajinder KUMAR Sangram KLENKA Debabrata PANDA Department of Biodiversity and Conservation of Natural resources Central University of OrissaKoraput 764021India ICAR-National Research centre on Plant biotechnology New Delhi 110012India TERI-Deakin Nanobiotechnology centre the Energy and Resources InstituteGurugramHaryana 122001India

The present study evaluated submergence responses in 88 lowland indigenous rice(Oryza sativa L.) landraces from Koraput, India, to identify submergence-tolerant rice genotypes. In pot experiments, variations in survival rate, shoot elongation, relative growth index, dry matter, chlorophyll, soluble sugar and starch contents were evaluated in two consecutive years under well-drained and completely submerged conditions. Principal component analysis showed that the first three axes contributed 96.820% of the total variation among the landraces, indicating wide variation between genotypes. Major traits such as survival rate, relative growth index, soluble sugar and starch contents appeared to be important determinants of phenotypic diversity among the landraces. Phenotypic coefficient of variance was higher than genotypic coefficient of variance for all the traits and all showed high heritability(90.38%–99.54%). Five rice landraces(Samudrabali, Basnamundi, Gadaba, Surudaka and Dokarakuji) were the most tolerant to submergence. When submerged for up to 14d, Samudrabali, Basnamundi and Godoba were notable for having greater survival rates than a standard submergence tolerant variety FR13 A, and also notable for elongating more vigorously and accumulating more biomass. These three landraces may therefore be especially useful in lowland rice growing areas that are affected by both moderate stagnant water and flash flooding. Molecular genotyping revealed that the submergence tolerance of Samudrabali, Basnamundi and Godoba is linked to the presence of one or more different Sub1 loci and it may well prove useful for breeding improved submergence tolerant rice varieties, thereby assising to improve yield stability in the rainfed lowland agro-ecosystem.

关键词： genetic variability genotyping indigenous rice submergence tolerance Sub1 gene

Development of a novel myconanomining approach for the recovery of agriculturally important elements from jarosite waste

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Journal of Environmental Sciences 2018年第5期30卷 356-367页

作者： Ankita Bedi Braj Raj Singh Sunil K.Deshmukh Nisha Aggarwal Colin J.Barrow Alok Adholeya TERI–Deakin Nanobiotechnology centre Biotechnology and Management of Bioresources Division The Energy and Resources Institute(TERI) School of Life and Environmental Sciences Deakin University Sri Aurobindo College Department of Chemistry University of Delhi

In this study, an ecofriendly and economically viable waste management approach have been attempted towards the biosynthesis of agriculturally important nanoparticles from jarosite waste. Aspergillus terreus strain J4 isolated from jarosite（waste from Debari Zinc Smelter,Udaipur, India）, showed good leaching efficiency along with nanoparticles（NPs） formation under ambient conditions. Fourier-transform infrared spectroscopy（FT-IR） and transmission electron microscopy（TEM） confirmed the formation of NPs. Energy dispersive X-ray spectroscopy（EDX analysis） showed strong signals for zinc, iron, calcium and magnesium,with these materials being leached out. TEM analysis and high resolution transmission electron microscopy（HRTEM） showed semi-quasi spherical particles having average size of 10‐50 nm. Thus, a novel biomethodology was developed using fungal cell-free extract for bioleaching and subsequently nanoconversion of the waste materials into nanostructured form. These biosynthesized nanoparticles were tested for their efficacy on seed emergence activity of wheat（Triticum aestivum） seeds and showed enhanced growth at concentration of 20 ppm. These nanomaterials are expected to enhance plant growth properties and being targeted as additives in soil fertility and crop productivity enhancement.

关键词： Bioleaching Fourier transform infraredspectroscopy (FTIR) Transmission electronmicroscopy (TEM) JarositeNanoparticles Seed-emergence activity

Elucidating Variations in Nitrogen Requirement According to Yield, Variety and Cropping System for Chinese Rice Production

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Pedosphere 2017年第2期27卷 358-363页

作者： ZHANG Yi CHEN Xinping MA Wenqi CUI Zhenling centre for resources Environment and Food Security China Agricultural University Beijing 100193 (China) College of Bioscience and biotechnology Hunan Agricultural University Changsha 410128 (China) College of resources and Environmental Science Hebei Agricultural University Baoding 071001 (China)

Better understanding of the factors that influence crop nitrogen(N) requirement plays an important role in improving regional N recommendations for rice(Oryza sativa L.) production. We collected data from 1 280 plot-level measurements in different reaches of the Yangtze River, China to determine which factors contributed to variability in N requirement in rice. Yield, variety, and cropping system were significantly related to N requirement. The N requirement remained consistent at about 18.6 kg N Mg^(-1)grain as grain yield increased from 7 to 9 Mg ha^(-1), then decreased to 18.1, 16.9, and 15.9 kg N Mg^(-1)grain as yield increased to 9–10, 10–11, and > 11 Mg ha^(-1), respectively. The decreased requirement for N with increasing yield was attributable to declining N concentrations in grain and straw and increased harvest index. Super rice variety had lower N requirement(17.7 kg N Mg^(-1)grain) than ordinary inbred and hybrid varieties(18.5 and 18.3 kg N Mg^(-1)grain, respectively), which was a result of lower grain and straw N concentrations of super rice. The N requirements were 19.2, 17.8, and 17.5 kg N Mg^(-1)grain for early, middle, and late rice cropping systems, respectively. In conclusion, the rice N requirement was affected by multiple factors, including yield, variety, and cropping system, all of which should be considered when planning for optimal N management.

关键词： grain and straw N concentrations grain yield harvest index N management plant N uptake reciprocal internalefficiency

Bioleaching of zinc from gold ores using Acidithiobacillus ferrooxidans

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International Journal of Minerals,Metallurgy and Materials 2009年第4期16卷 368-374页

作者： Pakawadee Kaewkannetra Francisco Jose Garcia-Garcia Tze Yen Chiu Department of biotechnology Faculty of TechnologyKhon Kaen University Corrosion and Protection centre School of MaterialsThe University of Manchester centre of Water Science Cranfield University

The present work investigated microbial leaching of zinc from ore using Acidithiobacillus ferrooxidans （ATCC 14859）. The ore samples, consisted of 13wt% zinc, were obtained from a gold mine in north-eastern Thailand. A shake flask study was performed on the ore samples using a rotary shaker under the following fixed conditions （250 r·min^-1, 30℃ for 16 d）. The influence of various conditions, namely medium type （with and without iron）, particle ore size （〈20, 20-40, 40-60, 60-100, and 〉100 mesh）, ore density （20 kg·m^-3, 50 kg·m^-3, and 100 kg·m^-3）, and pH of the medium （2, 2.5, 2.8, and 3）, were investigated. The microbial leaching was assessed by determining the concentration of zinc in the medium and compared with the initial sample concentration. The results show that Acidithiobacillus ferrooxidans can successfully leach zinc by as much as 6 times compared with the control experiment （without Acidithiobacillus ferrooxidans ferrooxidans）. The maximum efficiency （92.3%） for microbial leaching is obtained in iron-containing medium, 20-40 mesh ore sizes, 20 kg·m^-3 ore density at pH 2.8, and the zinc content is found in the medium at about 120 mg·m^-1.

关键词： bioleaching Acidithiobacillusferrooxidans gold ores gold mine

Chloroplast gene matK holds the barcodes for identification of Momordica(Cucurbitaceae) species from Indian subcontinent

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Horticultural Plant Journal 2022年第1期8卷 89-98页

作者： Girme Aoudumbar Ramesh Deepu Mathew K.Joseph John V.Ravisankarc centre for Plant biotechnology and Molecular Biology Kerala Agricultural UniversityThrissurKerala State 680656India ICAR-National Bureau of Plant Genetic resources Regional StationThrissurKerala State 680656India Bioinformatics centre Kerala Agricultural UniversityThrissurKerala State680656India

DNA barcoding is a supplementary tool in plant systematics,extensively used to resolve the species level *** paper details the identification of DNA barcodes for seven species of Momordica,using the chloroplast gene mat *** the species *** has been confused as a member of the genus Luffa,26 accessions of Momordica belonging to seven Indian species and two accessions of Luffa acutangula were included in this *** of mat K sequences has yielded distinct barcodes in *** ***,*** ***,***,***,*** and also in Luffa *** status of each species was reflected as nucleotide polymorphisms in each *** wild species *** and *** have yielded one barcode but failed to get ***,this study provides conclusive proof that *** is a member of Momordica *** phylogram generated was successful to distinguish the monoecious species of this genus,***,*** and ***,from the dioecious species ***,***,*** *** and ***,mat K locus,by accumulating the evolutionary sequence variations,is proven efficient to differentiate the Momordica species and to reveal their relatedness.

关键词： Cucurbit DNA barcoding Luffa Phylogeny systematics

Hybridization between salt resistant and salt susceptible genotypes of mungbean(Vigna radiata ***)and purity testing of the hybrids using SSRs markers

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Journal of Integrative Agriculture 2016年第3期15卷 521-527页

作者： Sehrawat Nirmala Yadav Mukesh Bhat Kangila Venkataraman Sairam Raj Kumar Jaiwal Pawan Kumar centre for biotechnology Maharshi Dayanand UniversityRohtak 124001India Department of biotechnology Maharishi Markandeshwar UniversityAmbala 133207India National Bureau of Plant Genetic resources Pusa CampusNew Delhi 110012India Indian Society for Plant Physiology G-3NASC ComplexPusaNew Delhi 110012India

Six cultivated and two wild genotypes of mungbean（Vigna radiata L. Wilczek） possessing variation for phenotypic and genotypic response for salt tolerance were hybridized. Hybridization results showed successful pod set and significant variations in cross-compatibility of investigated genotypes. Genotypes PLM 380 and PLM 562 showed promising combining ability with all genotypes. Results revealed significant crossing compatibility between V. radiata and V. sublobata. The cross ability ranged from 1.99 to 5.12%（average 3.08%）. Molecular analysis confirmed the hybrids purity. All F_1 seeds were bold, green/shiny green and germinated between 3 to 5 days. Hybrid plants were normal, fertile and healthier over their respective progenitors. Uniform flowering and maturity of the hybrids showed absence of any defect or alteration in plant habit and life cycle of the hybrids. The hybrid plants showed increase in yield characteristics as no. of pods, pod length, 100 seeds weight, and yield per plant. Number of pods containing F_2 seeds ranged from 5–8 per cluster. The F_2 seeds were collected and stored for further research. Present study suggests that salt resistant wild relatives or cultivars of mungbean can be explored by breeding as a source of useful traits/genes providing salt tolerance. This may help in development of best mungbean variety for saline prone areas. Micro-satellite markers linked to the trait/genes may assist breeding by early selection of the genotypes compared to the phenotypic screening.

关键词： mungbean salinity stress breeding hybrid purity improved characteristics