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ANAE染色和抗Thy 1-SPA菌体花环双标记法的建立及其用于检测小鼠T淋巴细胞的研究报告

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中国病理生理杂志 2000年第10期16卷 205-205页

作者：骆云鹏范维珂段积华李培然骆询 Boston CollegeofArtsandSciences!SuffolkUniversity USA MA02108 重庆医科大学病理生理教研室!重庆400016

Thy抗原[Thy(θ)antigen]是T淋巴细胞表面特异标志,系由第9对染色体等位基因编码的抗原分子.Mueller发现,抗θ血清可破坏ANAE阳性淋巴细胞,ANAE阴性者则否.

关键词：菌体双标记法淋巴细胞白细胞 ANAE Thy 1-SPA 小鼠小家鼠

肝癌患者尿多胺SPE-HPLC检测的临床应用价值

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中国病理生理杂志 2000年第10期16卷 195-196页

作者：骆云鹏王剂明尚京川林良方程平艳骆询康格非 CollegeofArts&sciences!SuffolkUniversity 重庆医科大学一院外科重庆医科大学检验系重庆医科大学基础医学院!重庆400016 MA02108 Boston

多胺(polymaines, PAs)为低分子直链非蛋白性含氮化合物,主要有腐胺(putrescine, Pu)、精脒(spermidine, Sd)和精胺(spermine, Sm).PAs源于L-鸟氨酸和L-蛋氨酸,与细胞生长、分化与增殖有密切关系,在生物大分子合成中起重要作用.

关键词：肝癌患者 SPE-HPLC 恶性肿瘤患者

临床恶性肿瘤研究中多胺SPE-ⅡPLC检测的价值

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中国病理生理杂志 2000年第10期16卷 284-284页

作者：骆云鹏王济明尚京川程平艳骆询傅渝滨康格非 MA02108 ChicagoUniversity DepartmentofMedicine UCLA 重庆医科大学临床学院!重庆400016 Boston SuffolkUniversity ForestPark DepartmentofPathology CollegeofArtsandSciences IL60130 CA90059-1617 重庆医科大学基础医学院!重庆400016

目的:多胺是反映细胞快速增殖的重要指标.本文拟利用多胺的此种生化特性,对恶性肿瘤患者尿液多胺变化进行测定与分析.方法:采用固相萃取(solid-phaseextraction, SPE)技术,改良人尿多胺高效液相色谱(HPLC)测定法;探讨常用抗癌药物(5-氟... 详细信息

目的:多胺是反映细胞快速增殖的重要指标.本文拟利用多胺的此种生化特性,对恶性肿瘤患者尿液多胺变化进行测定与分析.方法:采用固相萃取(solid-phaseextraction, SPE)技术,改良人尿多胺高效液相色谱(HPLC)测定法;探讨常用抗癌药物(5-氟脲嘧啶、长春新碱、卡铂、顺铂、VP16、放线菌素D、丝裂霉素C及柔红霉素等)对此法的干扰;检测并分析62例恶性肿瘤患者(包括36例可手术与26例不能手术的晚期恶性肿瘤)及28例健康人尿多胺水平变化;检测16例晚期肿瘤经化疗后及16例化疗初期患者之尿多胺值,并与26例晚期肿瘤化疗前尿多胺水平进行比较.结果:(1)可手术切除组与不能手术之晚期肿瘤组患者尿总PAs、Pu、Sm、及Sd水平和Sd/Sm、Pu/Sm比值均高于或明显高于健康人对照组(P＜0.05或P＜0.01).治疗一疗程后尿总PAs、Pu、Sd和Sm水平均降低(P＜0.05),而治疗之初期尿总PAs、Pu水平及Sd/Sm、Pu/Sm比值呈一过性增高(P＜0.05),其中后一指标持续到一疗程化疗后才接近治疗前水平.结论:固相萃取-高效液相色谱分析法(SPE-HPLC)用于尿PAs检测结果表明,恶性肿瘤患者尿PAs水平随病情的加重而增高,而经有效的手术及化疗后其值降低;此法准确性和分析效率高,适宜于临床恶性肿瘤患者诊断与化疗效果的监测.

关键词：多胺 SPE 恶性肿瘤患者晚期肿瘤

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便秘患者的生活质量问卷调查的开发与确认

世界核心医学期刊文摘（胃肠病学分册） 2005年第10期1卷 52-53页

作者： marquis P. De La Loge C. Dubois D. 郝筱倩 mapi Values 15 Court Square Boston MA 02108 United States Dr.

Objective. Chronic constipation is characterized by difficult, infrequent, or seemingly incomplete bowel movements. The Patient Assessment of Constipation Quality of Life (PACQOL) questionnaire was developed to address the need for a standardized, patient-reported outcomes measure to evaluate constipation over time. material and methods. Items for the PAC-QOL were generated from the literature, clinical experts, and patients. Following principal components and multitrait analyses, 28 items were retained forming four subscales (worries and concerns, physical discomfort, psychosocial discomfort, and satisfaction) and an overall scale. Validation studies were conducted in the United States, Europe, Canada, and Australia, to evaluate the internal consistency reliability (Cronbach’s alpha), reproducibility (Intraclass Correlation Coefficients (ICCs)), validity (analysis of variance models), and responsiveness (effect size) of the PAC-QOL scales. Results. The PAC-QOL scales were internally consistent (Cronbach’s alpha >0.80) and reproducible (ICCs > 0.70, except for the satisfaction subscale ICC = 0.66). PAC-QOL scale scores were significantly associated with abdominal pain (p < 0.001) and constipation severity (p < 0.05). Effect sizes in patients reporting improvements in constipation over a 6-week period were moderate to large, with subscale effect sizes ranging from 0.76 to 3.41 and the overall scale effect size = 1.77. Similar findings were observed in validation studies conducted in Europe, Canada, and Australia. Conclusions. The PAC-QOL is a brief but comprehensive assessment of the burden of constipation on patients’ everyday functioning and well-being. Multinational studies demonstrate that the PAC-QOL is internally consistent, reproducible, valid, and responsive to improvements over time.

关键词：便秘患者生活质量问卷慢性便秘患者信息严重度肠蠕动问卷调查临床专家指标评分心理障碍

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Special topic-Fisheries monitoring:Eyes in the ocean:Innovative and multidimensional solutions to monitor fisheries

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Aquaculture and Fisheries 2020年第3期5卷 105-106页

作者： Bowen Chang Shu Su Chongliang Zhang Yiping Ren Xiujuan Shan Jake Kritzer Yong Chen School of marine Sciences University of MaineOronoME 04469USA College of Fisheries Ocean University of ChinaQingdao266033China Yellow Sea Fisheries Research Institute Qingdao266071China Environment Defense Fund BostonMA02108USA

In the era of climate change,the visibility of environmental changes dictates public attention.Pictures of untamable bushfires,intense hurricanes,collapsing ice sheets are all gripping images that alarm us and urge us to take action.The ocean,however,gives us less such visuals;the changes that are taking place there are often abstract and hidden.However,the environmental challenges in the ocean are less visible but no less grave;they come from multiple sources:pollution,plastic waste,ocean surface warming,ocean acidification(IPCC,2019).But the most direct and age-old impact we exert on the ocean and its ecosystems is overfishing(FAO,2018).

关键词： Fisheries Monitoring program Special issue

Optically-generated focused ultrasound for noninvasive brain stimulation with ultrahigh precision

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Light(Science & Applications) 2022年第11期11卷 2817-2831页

作者： Yueming Li Ying Jiang Lu Lan Xiaowei Ge Ran Cheng Yuewei Zhan Guo Chen Linli Shi Runyu Wang Nan Zheng Chen Yang Ji-Xin Cheng Department of Mechanical Engineering Boston UniversityBostonMA 02215USA Graduate Program for Neuroscience Boston UniversityBostonMA 02215USA Department of Electrical and Computer Engineering Boston UniversityBostonMA 02215USA Department of Chemistry Boston UniversitBostonMA 02215USA Department of Biomedical Engineering Boston UniversityBostonMA 02215USA Division of materials Science and Engineering Boston UniversityBostonMA 02215USA Department of Biological Engineering Massachusetts Institute of TechnologyCambridgeMA 02142USA

High precision neuromodulation is a powerful tool to decipher neurocircuits and treat neurological diseases.Current non-invasive neuromodulation methods offer limited precision at the milimeter level.Here,we report opticallygenerated focused ultrasound(OFUS)for non-invasive brain stimulation with ultrahigh precision.OFUS is generated by a soft optoacoustic pad(SOAP)fabricated through embedding candle soot nanoparticles in a curved polydimethylsiloxane film.SOAP generates a transcranial ultrasound focus at 15 MHz with an ultrahigh lateral resolution of 83μm,which is two orders of magnitude smaller than that of conventional transcranial-focused ultrasound(tFUS).Here,we show effective OFUS neurostimulation in vitro with a single ultrasound cycle.We demonstrate submillimeter transcranial stimulation of the mouse motor cortex in vivo.An acoustic energy of 0.6 mJ/cm?,four orders of magnitude less than that of tFUS,is suffcient for successful OFUS neurostimulation.OFUS offers new capabilities for neuroscience studies and disease treatments by delivering a focus with ultrahigh precision noninvasively.

关键词： SOAP stimulation precision

Fisheries monitoring: Perspectives from the United States

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Aquaculture and Fisheries 2020年第3期5卷 131-138页

作者： Robert Boenish Daniel Willard Jacob P.Kritzer Kathleen Reardon Environmental Defense Fund 18 Tremont St.Suite 850BostonMA02108USA Environmental Defense Fund 301 Congress Ave.Suite 1300AustinTX78701USA maine Department of marine Resources 194 McKown Point RdWest Boothbay HarborME04575USA

Fisheries monitoring in the United States exists in many forms and serves many functions due to geographically varying objectives,practices,technology,institutional structures,and funding.In the U.S and abroad,diverse catch methods commonly exist for the same stock,thus monitoring and reporting strategies need to be tailored to unique operational needs.Common management challenges include funding limitation,survey design,coverage,and implementation.We describe three innovative examples of fisheries monitoring in the United States.These stories of success and failure can inform the design and implementation of new monitoring pilots and aid crafting both regional and national policies.We explore the innovative vessel monitoring and electronic logbook practices across multiple sectors for Gulf of Mexico red snapper(Lutjanus campechanus).Then,we examine a unique monitoring program that produces critical,near real-time genetic and population surveys for sockeye salmon(Oncorhynchus nerka)in Bristol Bay,Alaska.Our final case study describes the many fishery-dependent and-independent data streams for American lobster(Homarus americanus)in New England.Across all monitoring cases exists an explicit focus on the most critical aspects of organism life history.We find strong cross-institutional working relationships and adept agency coordination are imperatives in instances of stocks occupying multiple state or federal boundaries.Our results suggest the most effective approaches address the unique data needs of a fishery,and for this,thorough understanding of both biological and socioeconomic aspects of the fishery is a prerequisite.Ultimately,the monitoring program should jointly incentivize compliance while promoting continued and evolving interaction between resources users,scientists,and management.

关键词： Fisheries Fisheries monitoring Fisheries management Sustainability

Workability and Strength of Ceramsite Self-Compacting Concrete with Steel Slag Sand

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Journal of Renewable materials 2023年第2期11卷 881-904页

作者： Suiwei Pan Anqi Ren Yongli Peng Min Wu Wanguo Dong Chunlin Liu Depeng Chen School of Construction Engineering Ma’anshan UniversityMa’anshan243100China School of Architectural and Civil Engineering Anhui University of TechnologyMa’anshan243032China School of management Science and Engineering Anhui University of TechnologyMa’anshan243032China

This study focuses on the workability and compressive strength of ceramsite self-compacting concrete with fine aggregate partially substituted by steel slag sand(CSLSCC)to prevent the pollution of steel slag in the environment.The SF,J-ring,visual stability index,and sieve analysis tests are primarily employed in this research to investigate the workability of freshly mixed self-compacting concrete containing steel slag at various steel slag sand replacement rates.The experiment results indicate that CSLSCC with the 20%volume percentage of steel slag(VPS)performs better workability,higher strength,and higher specific strength.The 7-day compressive strength of CSLSCC with the 0.4 of the water-binder ratio(W/B),decreases with the increase of steel slag content,while the 28-day compressive strength increases significantly.The ceramsite self-compacting concrete with good comprehensive performance can be obtained when the substitution rate of steel slag sand for fine aggregate is less than 20%(volume percentage).

关键词： Steel slag ceramsite self-compacting concrete workability compressive strength

Bacterial extracellular vesicle applications in cancer immunotherapy

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Bioactive materials 2023年第4期22卷 551-566页

作者： Kanika Suri Anisha D’Souza Di Huang Aashray Bhavsar mansoor Amiji Department of Bioengineering College of EngineeringNortheastern UniversityBostonMA02115USA Department of Pharmaceutical Sciences School of PharmacyNortheastern UniversityBostonMA02115USA massachusetts Eye and Ear Infirmary Harvard Medical SchoolBostonMA20115USA Department of Chemical Engineering College of EngineeringNortheastern UniversityBostonMA02115USA

Cancer therapy is undergoing a paradigm shift toward immunotherapy focusing on various approaches to activate the host immune system.As research to identify appropriate immune cells and activate anti-tumor immunity continues to expand,scientists are looking at microbial sources given their inherent ability to elicit an immune response.Bacterial extracellular vesicles(BEVs)are actively studied to control systemic humoral and cellular immune responses instead of using whole microorganisms or other types of extracellular vesicles(EVs).BEVs also provide the opportunity as versatile drug delivery carriers.Unlike mammalian EVs,BEVs have already made it to the clinic with the meningococcal vaccine(Bexsero®).However,there are still many unanswered questions in the use of BEVs,especially for chronic systemically administered immunotherapies.In this review,we address the opportunities and challenges in the use of BEVs for cancer immunotherapy and provide an outlook towards development of BEV products that can ultimately translate to the clinic.

关键词： Cancer immunotherapy Bacterial extracellular vesicles Membrane vesicles Outer membrane vesicles mammalian extracellular vesicles