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Validation of Microchip Based RT-PCR ABC Test (InfA/B &COVID-19) in Clinical Samples

Journal of Biosciences and Medicines 2022年第8期10卷 172-187页

作者： Gabriel Martinez Ryan Nunley Michelle Gaines Timea Majoros Rajwant K. Gill Irina Gelimson Natallia Varankovich Maxim Slyadnev Sikander S. Gill 1CARE Medical Diagnostics Benicia CA USA lumex instruments canada Mission Canada

To contain the rapid and global spread of SARS-CoV-2, it is essential to develop an accurate and sensitive test system to address pandemic bottlenecks, simplified sample collection, and no sample prep. While meeting the demand of testing large populations, the miniaturized volume of assay reagents and offering rapid results is the need in such scenarios. Moreover, in view of the reports of co-infections and overlapping symptoms of Influenza caused by Influenza A or Influenza B, and COVID-19 caused by SARS-CoV-2, a test system with three targets can be supportive for accurate clinical diagnosis. In this presentation, we evaluated the performance of a test comprising Microchip RT-PCR Influenza and COVID-19 Detection System for identifying these three viral pathogens in nasal swabs and saliva specimens. A rapid and simplified total nucleic acid extraction method was developed and validated for the reliable, high-throughput simultaneous detection of respiratory viruses causing Influenza (type A and type B viruses) and COVID-19 (SARS-CoV-2 virus) using the microchip-based AriaDNATM platform deriving the name ABC Test. The test system was evaluated using 81 nasal swab samples, 77 clinical saliva samples, 5 blind CAP reference samples, and RNA standards. The limit of detection (LoD) was assessed using SARS-CoV-2, Influenza A, and Influenza B RNA standards. The multiplex ABC Test microchip displayed LoD of 14 copies/μL for SARS-CoV-2 and approximately 26 copies/μL for Influenza A, and 140 copies/μL for Influenza B, respectively. The ABC Test offers rapid multiplex one-step RT-PCR in 32 minutes for 45 cycles as the miniaturized reaction of 1.2 μL offering a highly sensitive, robust, and accurate assay for the detection of Influenza A/B, and SARS-CoV-2.

关键词： SARS-CoV-2 Influenza Multiplex Microchip PCR Nasal Swab and Saliva

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Identification and Quantitation of Cashmere (Pashmina) Fiber and Wool Using Novel Microchip Based Real-Time PCR Technology

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Journal of Textile Science and Technology 2018年第4期4卷 141-150页

作者： Rajwant Gill Sikander Gill Maxim Slyadnev Alexander Stroganov lumex instruments canada Mission Canada

The textile industrial chain all over the world is facing a challenge of differentiating cashmere fiber from mixture of wool and other fibers in case cashmere stocks are adulterated with wool or other fibers. For identification of cashmere in such mixtures, the development of microchip based real-time PCR technology offers a very sensitive, specific, and accurate solution. The technology has been validated with cashmere and wool samples procured from distant farms, and from cashmere goats and sheep of different age and sex. Model samples with incremental raw cashmere or wool content were tested. The experimentally determined content was found to be comparable to the weighed content of the respective fibers in the samples. This technology may prove a cost cutter since it needs only 1.2 μl of the PCR reagent mix. It is substantially faster than traditional real-time PCR systems for being carried as miniature reaction volume in metal microchip. These features allow faster thermal equilibrium and thermal uniformity over the entire array of microreactors. For routine tests or in commercial set up, the microchips are available as ready-to-run with lyophilized reagents in its microreactors to which only 1 μl of the 10-fold diluted isolated DNA sample is added. The lyophilized microchips offer user-friendly handling in testing laboratories and help minimize human error.

关键词： Microchip Real-Time PCR Identification Quantitation Cashmere Wool

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Validation of Microchip RT-PCR COVID-19 Detection System

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Journal of Biosciences and Medicines 2021年第9期9卷 8-24页

作者： Kelsey de Campos-Stairiker Asha Shravanthi Pidathala Rajwant K. Gill Irina Gelimson Natallia Varankovich Sikander S. Gill Maxim Slyadnev Sonia Kapur Ayumetrix Lake Oswego OR USA lumex instruments canada Mission BC Canada

While meeting the pandemic demand of SARS-CoV-2 testing, clinical laboratories worldwide tend to adopt new test systems offering cost-effective and faster test outcomes. However, the reliability of SARS-CoV-2 test results has paramount importance in the management of such a health crisis. Therefore, this study sought to determine the accuracy of the test results from a novel duplex Microchip RT-PCR test system using patient saliva samples and nasal swabs stabilized in Viral Transport Medium (VTM) with reference threshold Cycle Values (Ct). The VTM used to stabilize these samples during transport was found to be inhibitory to the RT-PCR. Therefore, all the samples were subjected to spin column purification of total RNA to remove the influence of VTM. A total of 70 patient samples, including 24 positive- and 31 negative-saliva in VTM samples and 15 positive nasal swab samples, were tested. Results obtained from both the sample types were compared to their reference values and no false positive or false negatives were observed. From this data, accuracy, specificity, and sensitivity were determined to be 100% applying the corresponding formulae. The limit of detection with 95% confidence probability was determined to be 2.5 copies/μl in the original sample.

关键词： SARS-CoV-2 Microchip RT-PCR Duplex Nasal Saliva LoD

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Color-Coding of Microchip RT-PCR Test System for SARS-CoV-2 Detection

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Journal of Biosciences and Medicines 2021年第5期9卷 94-119页

作者： Rajwant K. Gill Sikander S. Gill Irina Gelimson Maxim Slyadnev Gabriel Martinez Michelle Gaines Ryan Nunley Timea Majoros lumex instruments canada Mission BC Canada 1CARE Medical Diagnostics Benicia CA USA

An RT-PCR based microchip test system for the detection of SARS-CoV-2 offers pre-loaded and lyophilized reagents in the microchip. However, the 30- and 48-microwell formats of the microchip being miniaturized and performing 1.2 μl reaction, seek visual attention during sample addition. Therefore, adding colorants as color indicator in the lyophilized matrix in the microchips or adding to sample or master mix can impart not only user-friendliness to the task of liquid handling but also precision, and color-codes for easy identification of multiple kits in the layout of the microchip without compromising PCR data quality. A panel of colorants was screened for their background intensity, spectral inertness towards detection channels of AriaDNATM analyzer, interference with the reporter dyes (FAM, Cy5 and ROX), and visibility of optimal concentration in the microwell. The concentration of the colorant displaying insignificant impact on the quality of the amplification (Ct, fluorescence, and sensitivity) in comparison to no-colorant control was chosen for inclusion in the test kit. Tartrazine, Acid Red, Brilliant Blue and FAST Green colorants lyophilized with the reagents in the SARS-CoV-2 microchips were found to be stable and suitable. Storage of microchips with Fast Green colorant was tested at 40°C, 22°C, 4°C, and -20°C for 70 days and was found to be suitable and compatible with different master mixes available as liquid or lyophilized. Additionally, the microchips pre-loaded with lyophilized reagents in the presence and absence of two colorants Tartrazine and Fast Green were validated with clinical samples of SARS-COV-2. No significant impact of these colorants both intra- and inter-microchips was observed on the Ct and intensity of amplification for the tested samples in comparison to no-colorant control. The data su

关键词： SARS-CoV-2 Colorants Microchip PCR 2-Plex

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Ground-based and additional science support for SMILE

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Earth and Planetary Physics 2024年第1期8卷 275-298页

作者： J.A.Carter M.Dunlop C.Forsyth K.Oksavik E.Donovon A.Kavanagh S.E.Milan T.Sergienko R.C.Fear D.G.Sibeck M.Connors T.Yeoman X.Tan M.G.G.T.Taylor K.McWilliams J.Gjerloev R.Barnes D.D.Billet G.Chisham A.Dimmock M.P.Freeman D.-S.Han M.D.Hartinger S.-Y.W.Hsieh Z.-J.Hu M.K.James L.Juusola K.Kauristie E.A.Kronberg M.Lester J.Manuel J.Matzka I.McCrea Y.Miyoshi J.Rae L.Ren F.Sigernes E.Spanswick K.Sterne A.Steuwer T.Sun M.-T.Walach B.Walsh C.Wang J.Weygand J.Wild J.Yan J.Zhang Q.-H.Zhang Planetary Sciences Group School of Physics and AstronomyUniversity of LeicesterUniversity RoadLeicesterLE17RHUK Rutherford Appleton Laboratory Space Science Technology Facilities CouncilOxfordshireUK School of Space and Environment Beihang UniversityBeijing 100191China Key Laboratory of Space Environment Monitoring and Information Processing Ministry of Industry and Information TechnologyBeijing 100017China Department of Space and Climate Physics UCLMullard Space Science LaboratoryHolmbury St.MaryDorkingSurreyRH56NTUK Birkeland Centre for Space Science Department of Physics and Technology.University of BergenBergenNorway Department of Physics and Astronomy University of Calgary2500 University DriveCalgaryAlbertaCanada T2N 1N4 British Antarctic Survey CambridgeUK Swedish Institute of Space Physics KirunaSweden West Highfield Campus University of SouthamptonUniversity RoadSO171BJUK Goddard Space Flight Center NASAGreenbeltMarylandUSA Athabasca University AthabascaCanada ESTEC European Space AgencyNetherlands University of Saskatchewan SaskatoonCanada The Johns Hopkins University Applied Physics Laboratory LaurelMarylandUSA Swedish Institude of Space Physics UppsalaSE 75121Sweden Tongji University Shanghai 200092China Center for Space Plasma Physics Space Science Institute4765 Walnut Street Suite BBoulderColorado80301USA Polar Research Institute of China Shanghai 200136China Finnish Meteorological Institute(FMI) Finland Department of Earth and Environmental Sciences(Geophysics) Ludwig Maximilian University of Munich(LMU)MunichTheresienstr.41MunichD-80333Germany Canadian Space Agency MontrealCanada GFZ German Research Centre for Geosciences PotsdamGermany Nagoya University Institute for Space Earth Environment ResearchCenter for Integrated Data ScienceNagoyaAichiJP 464-8601 Northumbria University Newcastle upon TyneTyne and WearUK NE18ST National Space Science Center Chinese Academy SciencesBeijing 100190China Arctic Geophysics University Centre in Svalbard

The joint European Space Agency and Chinese Academy of Sciences Solar wind Magnetosphere Ionosphere Link Explorer(SMILE)mission will explore global dynamics of the magnetosphere under varying solar wind and interplanetary magnetic field conditions,and simultaneously monitor the auroral response of the Northern Hemisphere *** these large-scale responses with medium and fine-scale measurements at a variety of cadences by additional ground-based and space-based instruments will enable a much greater scientific impact beyond the original goals of the SMILE ***,we describe current community efforts to prepare for SMILE,and the benefits and context various experiments that have explicitly expressed support for SMILE can offer.A dedicated group of international scientists representing many different experiment types and geographical locations,the Ground-based and Additional Science Working Group,is facilitating these *** include constructing an online SMILE Data Fusion Facility,the discussion of particular or special modes for experiments such as coherent and incoherent scatter radar,and the consideration of particular observing strategies and spacecraft *** anticipate growing interest and community engagement with the SMILE mission,and we welcome novel ideas and insights from the solar-terrestrial community.

关键词： magnetosphere ionosphere magnetosphere-ionosphere coupling ground-based experimentation SMILE conjunctions missions

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Photographic Analysis and Optical Diagnosis of Kilowatt Microwave Plasma Torch with Air Carrier Gas

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Chemical Research in Chinese Universities 2023年第6期39卷 1051-1057页

作者： YU Dengjie YU Bingwen ZHANG Xuchen HUANG Shiluo YING Yangwei YAN Yuwei JIN Yining JIN Wei Research Center for Analytical Instrumentation Institute of Cyber-Systems and ControlCollege of Control Science and EngineeringZhejiang UniversityHangzhou 310058P.R.China Research Center for Analytical instruments and Intelligent Systems Huzhou Institute of Zhejiang UniversityHuzhou 313002P.R.China Faculty of Engineering University of Alberta116 St.and 85 Ave.EdmontonAlbertaCanada

The spatiotemporal motion characteristics of the kilowatt argon microwave plasma torch with the air carrier gas(kW-AC-ArMPT)and the behavior of the plasma filaments are investigated with a digital single-lens reflex(SLR)camera and a high-speed *** with the introduction of the air,both the volume of the central channel and the rotational frequency of the plasma filament are ***,the excitation temperature(Texc),rotational temperature(Trot),and density of electron number(ne)of the kW-AC-ArMPT are measured with optical *** is clearly shown that the introduction of air contributed to the rise of Trot and ne of the plasma,which is beneficial to improving the analytical performance of the *** the detection limits of some heavy metal elements are measured by kW-AC-ArMPT,which are in the ppb *** experimental results show that the kW-ArMPT has a high tolerance to air injection at least 1.0 L/min,which allows the direct extraction of air from the environment for analysis and therefore has the potential for online and in-situ detection of ambient air quality and industrial exhaust gases.

关键词： Air carrier gas Microwave plasma torch Photographic analysis Optical diagnosis

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Simultaneous, hybrid single-molecule method by optical tweezers and fluorescence

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Nanotechnology and Precision Engineering 2019年第4期2卷 145-156页

作者： Guoteng Ma Chunguang Hu Shuai Li Xiaoqin Gao Hongbin Li Xiaotang Hu State Key Laboratory of Precision Measuring Technology and instruments Tianjin University300072China Nanchang Institute for Microtechnology of Tianjin University Tianjin 300072China Department of Chemistry University of British ColumbiaVancouverBC V6T 1Z1Canada

As studies on life sciences progress toward the single-molecule level,new experiments have put forward more requirements for simultaneously displaying the mechanical properties and conformational changes of *** tweezers and fluorescence microscopy have been combined to solve this *** combination of instruments forms a new generation of hybrid single-molecule technology that breaks through the limitations of traditional biochemical *** and fluorescence visualization have beenwidely used,and these techniques provide new possibilities for studying complex biochemical reactions at the singlemolecule *** paper explains the features of this combined technique,including the application characteristics of single-trap and dual-traps,the anti-bleaching method,and optical tweezers combined with epifluorescence,confocal fluorescence,total internal reflection fluorescence,and other fluorescence *** typical experiments,we analyze technical solutions and explain the factors and principles that instrument designers should *** review aims to give an introduction to this novel fusion technology process and describe important biological results.

关键词： Single-molecule Optical tweezers Confocal fluorescence FRET TIRF

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Force application of laparoscopic surgeons under the impact of heavy personal protective equipment during COVID-19 pandemic

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Laparoscopic, Endoscopic and Robotic Surgery 2022年第3期5卷 106-110页

作者： Yao Zhang Shuyi(Kiana)Wang Bin Zheng Surgical Simulation Research Lab University of AlbertaEdmontonAlbertaCanada

Objective:Surgeons are required to wear heavy personal protective equipment while delivering care to patients during the COVID-19 *** examined the impact of wearing double gloves on surgeons’performance in laparoscopic ***:Eleven surgeons-in-training at the Surgical Simulation Research Lab of the University of Alberta were recruited to perform laparoscopic cutting tasks in simulation while wearing none,one pair,and two pairs of surgical *** applied to laparoscopic instruments were ***:Wearing gloves prolonged task times(one pair of gloves:301.6±61.7 s;two pairs of gloves:295.8±65.3 s)compared with no gloves(241.7±46.9 s;p=0.043).Wearing double gloves increased cutting errors(20.4±5.1 mm^(2))compared with wearing one pair of gloves(16.9±5.5 mm^(2))and no gloves(14.4±4.6 mm^(2);p=0.030).Wearing gloves reduced the peak force(one pair of gloves:2.4±0.7 N;two pairs of gloves:2.7±0.6 N;no gloves:3.4±1.4 N;p=0.049),and the total force(one pair of gloves:10.1±2.8 N;two pairs of gloves:10.3±2.6 N;no glove:12.6±1.9 N;p=0.048)delivered onto lapa-roscopic scissors compared with wearing no ***:The combined effects of wearing heavy gloves and using tools reduced the touching sensation,which limited the surgeons’confidence in performing surgical *** practice in simulation is suggested to allow surgeons to overcome difficulties brought by personal protective equipment.

关键词： Personal protective equipment Surgical care Force application Errors Patient safety Simulation

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Fucoidan and topography modification improved in situ endothelialization on acellular synthetic vascular grafts

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生物活性材料(英文) 2023年第4期22卷 535-550页

作者： Yuan Yao Aung Moe Zaw Deirdre E.J.Anderson YeJin Jeong Joshua Kunihiro Monica T.Hinds Evelyn K.F.Yim Department of Chemical Engineering University of Waterloo200 University Avenue WestWaterlooONN2L 3G1Canada Department of Biomedical Engineering Oregon Health&Science UniversityPortlandOR97239USA Waterloo Institute for Nanotechnology University of Waterloo200 University Avenue WestWaterlooONN2L 3G1Canada Center for Biotechnology and Bioengineering University of Waterloo200 University Avenue WestWaterlooONN2L 3G1Canada

Thrombogenesis remains the primary failure of synthetic vascular *** coverage is crucial to provide an antithrombogenic ***,most synthetic materials do not support cell adhesion,and transanastomotic endothelial migration is ***,a surface modification strategy using fucoidan and topography was developed to enable fast in situ endothelialization of polyvinyl alcohol,which is not endothelial *** three different immobilization approaches compared,conjugation of aminated-fucoidan promoted endothelial monolayer formation while minimizing thrombogenicity in both in vitro platelet rich plasma testing and ex vivo non-human primate shunt *** of six topographical patterns showed that 2μm gratings increased endothelial cell migration without inducing inflammation responses of endothelial *** studies demonstrated that fucoidan could attract fibronectin,enabling integrin binding and focal adhesion formation and activating focal adhesion kinase(FAK)signaling,and 2μm gratings further enhanced FAK-mediated cell *** a clinically relevant rabbit carotid artery end-to-side anastomosis model,60%in situ endothelialization was observed throughout the entire lumen of 1.7 mm inner diameter modified grafts,compared to 0%of unmodified graft,and the four-week graft patency also *** work presents a promising strategy to stimulate in situ endothelialization on synthetic materials for improving long-term performance.

关键词： Blood-contacting devices Small diameter vascular graft Endothelialization Topography Fucoidan

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Diffractive optical elements 75 years on:from micro-optics to metasurfaces

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Photonics Insights 2023年第4期2卷 43-101页

作者： Qiang Zhang Zehao He Zhenwei Xie Qiaofeng Tan Yunlong Sheng Guofan Jin Liangcai Cao Xiaocong Yuan Nanophotonics Research Center Institute of Microscale Optoelectronics&State Key Laboratory of Radio Frequency Heterogeneous IntegrationShenzhen UniversityShenzhenChina Department of Precision instruments Tsinghua UniversityBeijingChina Center for Optics Photonics and LasersLaval UniversityQuebecCanada Research Institute of Intelligent Sensing Research Center for Humanoid SensingZhejiang LabHangzhouChina

Diffractive optical elements(DOEs)are intricately designed devices with the purpose of manipulating light fields by precisely modifying their *** concept of DOEs has its origins dating back to 1948 when *** first introduced ***,researchers introduced binary optical elements(BOEs),including computer-generated holograms(CGHs),as a distinct category within the realm of *** was the first revolution in optical *** next major breakthrough in light field manipulation occurred during the early 21st century,marked by the advent of metamaterials and *** are particularly appealing due to their ultra-thin,ultra-compact properties and their capacity to exert precise control over virtually every aspect of light fields,including amplitude,phase,polarization,wavelength/frequency,angular momentum,*** advancement of light field manipulation with micro/nano-structures has also enabled various applications in fields such as information acquisition,transmission,storage,processing,and *** this review,we cover the fundamental science,cutting-edge technologies,and wide-ranging applications associated with micro/nano-scale optical devices for regulating light *** also delve into the prevailing challenges in the pursuit of developing viable technology for real-world ***,we offer insights into potential future research trends and directions within the realm of light field manipulation.

关键词： diffractive optical elements metasurfaces metamaterials

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