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Cultivar-dependent rhizobacteria community and cadmium accumulation in rice: Effects on cadmium availability in soils and iron-plaque formation

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Journal of Environmental Sciences 2022年第6期34卷 90-102页

作者： Feng Zhang Di Peng Lu Liu Huidan Jiang Lianyang Bai longping branch Graduate School of Hunan UniversityChangsha 410125China Biotechnology Research Institute Hunan Academy of Agricultural SciencesChangsha 410125China

The association between the rhizospheric microbial community and Cd accumulation in rice is poorly understood.A field trial was conducted to investigate the different rhizobacterial communities of two rice cultivars with high Cd accumulation(HA)and low Cd accumulation(LA)at four growth stages.Results showed that the Cd content in the roots of the HA cultivar was 1.23-27.53 higher than that of the LA cultivar(0.08-10.5μg/plant)at four stages.The LA cultivar had a significantly lower Cd availability in rhizosphere and a higher quantity of iron plaque(IP)on the root surface than the HA cultivar at four stages.This resulted in the reduction of Cd concentration in IPs and Cd translocation from IP-to-root.Microbial analysis indicated that the LA cultivar formed a distinct rhizobacterial community from the HA cultivar and had lessα-diversity.The rhizosphere of the LA cultivar was enriched in specific bacterial taxa(e.g.,Massilia and Bacillus)involved in Cd immobilization by phosphate precipitation and IP formation by iron oxidization.However,the rhizosphere in the HA cultivar assembled abundant sulfur-oxidizing bacteria(e.g.,Sulfuricurvum)and iron reduction bacteria(Geobacter).They promoted Cd mobilization and reduced IP formation via the metal redox process.This study reveals a potential approach in which specific rhizobacteria decrease or increase Cd accumulation in rice on contaminated soil and provides a new perspective for secure rice production.

关键词： Rice cultivar Cd accumulation Rhizosphere Bacterial community Iron plaque Cd availability

A novel single-base mutation in CaSGR1 confers the stay-green phenotype in pepper

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Horticultural Plant Journal 2023年第2期9卷 293-305页

作者： Yuhua Liu Lijun Ou Zhoubin Liu Junheng Lyu JingWang Jingshuang Song Bozhi Yang Wenchao Chen Sha Yang Wei Liu Xuexiao Zou Zhuqing Zhang College of Life Sciences and Environment Hengyang Normal UniversityHengyangHenan 421008China Vegetable Institution of Hunan Academy of Agricultural Science ChangshaHunan 410125China College of Horticulture Hunan Agricultural UniversityChangshaHunan 410128China longping branch Graduate School of Hunan UniversityChangshaHunan 410125China

The stay-green trait is of considerable importance in extending the shelf life of green pepper fruit(Capsicum annuum L.)and in enhancing the appearance of ornamental plants.The study revealed the genetic and regulatory mechanisms of the stay-green trait in pepper,which will aid in the selection of ornamental pepper varieties.In this study,a pepper mutant with stay-green fruit named TNX348 was identified from a germplasm resource bank.Two segregating populations were constructed using the stay-green mutant TNX348 and then used in bulked segregant analysis combined with RNA sequencing and linkage analyses.The causal gene of the stay-green trait was mapped to an approximately 131-kb region,and a senescence-induced chloroplast protein gene,CaSGR1(Capana01g000359),was identified as a candidate gene.Sequencing analysis revealed a G→A single-base mutation of CaSGR1 in TNX348 that led to early termination of translation.Based on the single-base mutation,a single nucleotide polymorphism(SNP)marker co-segregating with the stay-green trait was developed.Furthermore,in transcriptome analysis,expression patterns of 11 hormone transduction-related transcription factors,such as abscisic acid-insensitive(ABI),abscisic acidresponsive element-binding factor(ABF),and NAC transcription factor,were similar or opposite to that of CaSGR1.The results indicated that the transcription factors might mediate chlorophyll degradation by regulating the expression of CaSGR1.

关键词： Pepper Stay-green trait Chlorophyll degradation CaSGR1 Hormone transduction-related transcription factors

Development and Application of a Novel Functional Marker for Fragrance in Rice

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Rice science 2023年第3期30卷 176-180,I0014页

作者： SUN Pingyong ZHANG Wuhan ZHANG Li SHU Fu HE Qiang XU Na PENG Zhirong ZENG Jia FANG Pengpeng DENG Huafeng State Key Laboratory of Hybrid Rice/Hunan Hybrid Rice Research Center Hunan Academy of Agricultural SciencesChangsha 410125China longping branch College of BiologyHunan UniversityChangsha 410125China Nuclear Agriculture and Space Breeding Research Institute Changsha 410125China

Fragrant rice is favored worldwide by consumers and rice breeders,due to its full aroma and high nutritional value.Loss of function of the betaine aldehyde dehydrogenase gene(OsBADH2)leads to aroma in rice.Our previous sequencing results showed that fragrant rice Chuan 7 belongs to badh2-E14 type,with a 1 bp insertion mutation in exon 14(Sun et al,2021),and this allele mutation is mainly distributed in Nepal and India(Kovach et al,2009).

关键词： Marker insertion breed

A global view of transcriptome dynamics during flower development in Capsicum annuum L.

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Horticultural Plant Journal 2023年第5期9卷 999-1012页

作者： Bingqian Tang Huiping Yang Xinhao Zhang Juan Du LingLing Xie Xiongze Dai Xuexiao Zou Feng Liu Engineering Research Center of Education Ministry for Germplasm Innovation and Breeding New Varieties of Horticultural Crops Key Laboratory for Vegetable Biology of Hunan ProvinceCollege of HorticultureHunan Agricultural UniversityChangsha 410125China longping branch Graduate School of Hunan UniversityChangshaHunan 410125China College of Horticulture Nanjing Agricultural UniversityNanjingJiangsu 210095China

An analysis of genome-wide gene expression profiles during floral organ development can provide important clues about the molecular basis of gene functions and developmental processes.In this study,we analyzed the transcriptome data of 36 samples obtained during floral organ development from pepper‘6421’and detected 30016 genes that were expressed in at least one sample.K-means clustering analysis was used to classify the data into 16 clusters based on the similarities between the dynamic expression profiles of genes.Of these,15 clusters exhibited notable up-regulation or down-regulation trends in different developmental stages or tissues of floral organs.We identified transcription factors expressed at the early,medium,and late stages of bud development(F1,F5,F9).Transcription factor families such as AP2-ERF,MADS-box,MYB,bHLH,and NAC showed significant levels of enrichment.In comparison with genes expressed in vegetative tissues at different stages,certain genes were specifically up-regulated during flower development;among these,the number of genes specifically up-regulated during the stamen(Sta10)and bud tetrad development(F4)stages was the highest.Through extensive studies of the ABCDE model of flower development in Arabidopsis,we identified 17 ABCDE model candidate genes in pepper,most of which were up-regulated at specific stages of flower bud development.The expression data provided in this study is the most comprehensive dataset available for pepper to date and will serve as a resource for identifying the functions of many specific genes involved in flower development in pepper and other Solanaceae plants.

关键词： Pepper Transcriptome Flower development Period-specific expression Transcription factor ABCDE model

Hybrid rice:achievement,challenges and opportunity

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Science Bulletin 2017年第17期62卷 1163-1164页

作者： Fangming Xie Yuan longping High-Tech Agriculture Co. LtdChangsha 410000China

In commemoration of the publication of Professor Yuan longping’s article,‘‘Rice Male Sterility',Chinese Science Bulletin produced a special issue on hybrid rice for its 50th anniversary[1].This special issue reviewed and summarized all breakthroughs,achievements,and research progress made in the last 50 years for hybrid rice in China.From the simple concept of using male sterility to explore heterosis in rice,hybrid rice development

关键词：中国杂交水稻雄性不育系三系杂交水稻水稻生产杂种优势细胞水平籼粳杂交恢复系

The osmolyte-producing endophyte Streptomyces albidoflavus OsiLf-2 induces drought and salt tolerance in rice via a multi-level mechanism

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The Crop Journal 2022年第2期10卷 375-386页

作者： Shuqi Niu Yan Gao Huixian Zi Ying Liu Xuanming Liu Xianqiu Xiong Qingqing Yao Ziwei Qin Ning Chen Liang Guo Yuanzhu Yang Peng Qin Jianzhong Lin Yonghua Zhu Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation College of BiologyHunan UniversityChangsha 410008HunanChina Yuan longping High-Tech Agriculture Co. Ltd.Changsha 410001HunanChina

Drought and salinity are major environmental stresses that impair crop growth and productivity worldwide. Improving drought and salt tolerance of crops with microbial mutualists is an effective and environmentally sound strategy to meet the demands of the ever-growing world population. In the present study, we found that the Streptomyces albidoflavus Osi Lf-2, a moderately salt-tolerant endophytic actinomycete, produced abundant osmolytes, including proline, polysaccharides, and ectoine. Inoculation with Osi Lf-2 increased the osmotic-adjustment ability of the rice host by increasing the proline content(by250.3% and 49.4%) and soluble sugar(by 20.9% and 49.4%) in rice under drought and salt conditions, relative to the uninoculated control. Osi Lf-2 increased stress responses in the rice host at the physiological and biochemical levels(photosynthesis efficiency, osmolytes and antioxidant content), and the gene level(osmolytes synthesis, stress-responsive and ion-transport related genes), raising rice yields under both greenhouse and saline–alkaline soil conditions. The use of endophytic actinomycetes offers a promising biotechnological approach to developing stress-tolerant plants.

关键词： Rice Endophytic actinomycete Osmolytes Salt tolerance Drought tolerance

Construction of chimeric viruses based on pepper mild mottle virus using a modiffed Cre/loxP system

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Journal of Integrative Agriculture 2022年第8期21卷 2456-2463页

作者： YIN Yue-yan HUA Meng-ying ZHAO Kuang-jie WAN Qiong-lian BU Shan LU Yu-wen ZHENG Hong-ying RAO Shao-fei YAN Fei PENG Jie-jun CHEN Hai-ru CHEN Jian-ping College of Plant Protection Yunnan Agricultural UniversityKunming 650201P.R.China Institute of Alpine Economic Plants Yunnan Academy of Agricultural SciencesLijiang 674100P.R.China State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts Institute of Plant VirologyNingbo UniversityNingbo 315211P.R.China longping branch of Graduate College Hunan UniversityChangsha 410125P.R.China

Cre/loxP,a site-specific recombination system,has been widely used for various purposes,including chromosomal translocations,generation of marker-free transgenic plants,tissue-specific activation of a reporter gene and efficient heterologous gene expression in plants.However,stable or transient expression of Cre recombinase in plants can cause chlorosis or necrosis.Here,we describe a modified Cre/loxP recombination system using a DNA fragment flanked with loxP sites in the same orientation in which necrosis induced by Cre recombinase in Nicotiana benthamiana leaves was alleviated.The modified system was successfully used to create functional GFP-tagged pepper mild mottle virus(PMMoV)and a chimeric virus with coat protein(CP)substitution assembled from separate pro-vector modules.Our results provide a new strategy and flexible technique to construct chimeric virus and infectious clones for plant viruses with large genomes.

关键词： pepper mild mottle virus Cre/loxP necrosis infectious cDNA clone chimeric virus

Rapid CO_(2)-laser scribing fabrication of an electrochemical sensor for the direct detection of Pb^(2+) and Cd^(2+)

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Nano Research 2023年第5期16卷 7671-7681页

作者： Guanglei Chu Yanyan Zhang Zhongrui Zhou Weixuan Zeng Dongfei Chen Siping Yu Jiemin Wang Yemin Guo Xia Sun Ming Li Hunan Agricultural Equipment Research Institute Hunan Academy of Agricultural SciencesChangsha 410125China School of Agriculture Engineering and Food Science Shandong University of TechnologyZibo 255049China School of Chemistry University of New South WalesSydneyNSW 2052Australia Hunan Rice Research Institute Hunan Academy of Agricultural SciencesChangsha 410125China longping branch College of BiologyHunan UniversityChangsha 410125China Department of Materials Science and Engineering National University of SingaporeNo.21 Lower Kent Ridge RoadSingapore 119077Singapore

Laser-induced graphene(LIG)is a highly promising preparation material for electrochemical sensors;however,its preparation speed and nanomaterial modification steps significantly limit its mass production.Herein,this study proposed a new laser printing strategy that considerably improved the preparation speed of LIG with excellent electrochemical performance.Using the optimal parameters(laser power of 1%,scribing spacing of 0.12 mm,scribing speed of 100 mm·s^(−1)),it took only 14.2 s to complete the preparation of the detection electrode.Thus,we successfully detected Cd^(2+)and Pb^(2+)without any toxic reagents or electrode modification steps.The limits of detection of the sensor were 0.914 and 0.916μg·L^(−1)for Cd^(2+)and Pb^(2+),respectively,which are significantly lower than the required values for drinking-water quality,according to the World Health Organization guidelines.This study provides a novel approach for the rapid detection of heavy-metal ions.

关键词： laser-induced graphene electrochemical sensor rapid fabrication heavy-metal ions

Novel structural annotation and functional expression analysis of GTP_EFTU conserved genes in pepper based on the PacBio sequencing data

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Horticultural Plant Journal 2021年第5期7卷 443-456页

作者： Bingqian Tang Lingling Xie Xuefeng Li Huiping Yang Yi Liu Xiang Cheng Chenliang Liang Juan Chen Shudong Zhou Duanhua Wang Jingyuan Zheng Xiongze Dai Xuexiao Zou Feng Liu longping branch Graduate School of Hunan UniversityChangshaHunan 410125China Vegetable Institution of Hunan Academy of Agricultural Science ChangshaHunan 410125China College of Landscape and Horticulture Hunan Agricultural UniversityChangshaHunan 410125China

Genes containing GTP_EFTU domain mainly express elongation factors(EF),Small GTPases,and GTP-binding proteins,which are closely related to protein synthesis,extension and ATP synthesis.In this study,we identified 39 genes containing GTP_EFTU domains from peppers.The evolutionary trees constructed from capsicum,Arabidopsis,rice,and tomato are mainly divided into 7 subfamilies.Using PacBio(Pacific Biosciences)sequencing and assembly data,we extracted these 39 gene sequences,fromwhich 25 genes had alternative splicing.Particularly,the Capana08g000545 had 16 alternative splicing processes.Accordingly,we performed promoter sequence analysis,subcellular location prediction,the expression analysis of different tissues and periods,and also the GO(Gene ontology)analysis of co-expressed genes.Lastly we did the qRTPCR analysis in 5 stages of pepper fruit development.These analyses revealed important structural and functional information for the identified 39 genes that contain GTP_EFTU domains,providing important references for further follow-up experiments to verify the genes function on plants or their unique roles in peppers.

关键词： Capsicum GTP_EFTU PacBio Phylogenetic analysis Expression analysis GO analysis