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Protective action of NADPH oxidase inhibitors and role of NADPH oxidase in pathogenesis of colon inflammation in mice

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World Journal of Gastroenterology 2014年第35期20卷 12533-12541页

作者： Rima Ramonaite Jurgita Skieceviciene Simonas Juzenas Violeta Salteniene Juozas Kupcinskas Paulius Matusevicius Vilmante Borutaite Limas Kupcinskas Institute for Digestive Research Academy of MedicineLithuanian University of Health Sciences Department of Gastroenterology Academy of MedicineLithuanian University of Health Sciences Department of Animal Nutrition Academy of VeterinaryLithuanian University of Health Sciences Institute for Neurosciences Academy of MedicineLithuanian University of Health Sciences

AIM:To investigate the role of nicotinamide adenine dinucleotide phosphate(NADPH) oxidase in colon epithelial cells in the pathogenesis of acute and chronic colon inflammation in a mouse model of dextran sulphate sodium(DSS)-induced colitis.METHODS:Balb/c mice were divided into three groups:8 mice with acute DSS-induced colitis(3.5% DSS solution;7 d),8 mice with chronic DSS-induced colitis(3.5% DSS solution for 5 d + water for 6 d;4 cycles;total:44 d) and 12 mice without DSS supplementation as a control group.Primary colonic epithelial cells were isolated using chelation method.The cells were cultivated in the presence of mediators(lipopolysaccharide(LPS),apocynin or diphenyleneiodonium).Viability of cells was assessed by fluorescent microscopy.Production of reactive oxygen species(ROS) by the cells was measured fluorometrically using Amplex Red.Production of tumour necrosis factor-alpha(TNF-α) by the colonic epithelial cells was analysed by ELISA.Nox1 gene expression was assessed by real-time PCR.RESULTS:Our study showed that TNF-α level was increased in unstimulated primary colonic cells both in the acute and chronic colitis groups,whereas decreased viability,increased ROS production,and expression of Nox1 was characteristic only for chronic DSS colitis mice when compared to the controls.The stimulation by LPS increased ROS generation via NADPH oxidase and decreased cell viability in mice with acute colitis.Treatment with NADPH oxidase inhibitors increased cell viability and decreased the levels of ROS and TNF-α in the LPS-treated cells isolated from mice of both acute and chronic colitis groups.CONCLUSION:Our study revealed the importance of NADPH oxidase in the pathogenesis of both acute and chronic inflammation of the colon.

关键词： Apocynin Diphenyleneiodonium Dextran sulphate sodi

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Acute phase reactants, challenge in the near future of animal production and veterinary medicine

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2005年第10期6卷 941-947页

作者： GRUYS E. ,TOUSSAINT M.J.M. ,UPRAGARIN N. ,VAN EDEREN A.M. ,ADEWUYI A.A. ,CANDIANI D. ,NGUYEN T.K.A. ,SABECKIENE (BALCIUTE) J. the Netherlands Department of Pathobiology Faculty of veterinary Medicine Utrecht University Utrecht the Netherlands Department of Pathobiology Faculty of veterinary Medicine Utrecht University Utrecht Department of Pathobiology the Netherlands Department of Pathobiology Faculty of veterinary Medicine Utrecht University Utrecht the Netherlands Department of Pathobiology Faculty of veterinary Medicine Utrecht University Utrecht Faculty of veterinary Medicine the Netherlands Department of Pathobiology Faculty of veterinary Medicine Utrecht University Utrecht the Netherlands Department of Pathobiology Faculty of veterinary Medicine Utrecht University Utrecht Utrecht University the Netherlands Department of Farm Resources and Production Medicine Faculty of veterinary Medicine Kasetsart University Nakhon Pathom Thailand Department of veterinary Morphophysiology University of Torino Italy Department of Embryo Transfer and Reproduction the National Institute of Animal Husbandry Utrecht Hanoi Vietnam Department of Infectious Diseases lithuanian veterinary academy Kaunas Lithuania) the Netherlands Department of Pathobiology Faculty of veterinary Medicine Utrecht University Utrecht

The future of acute phase proteins (APPs) in science is discussed in this paper. Many functions and associated pathological processes of APPs are unknown. Extrahepatic formation in local tissues needs attention. Local serum amyloid A (SAA) formation may be involved in deposition of AA-amyloid induced by conformational change of SAA resulting in amyloid formation, having tremendous food safety implications. Amyloidogenesis is enhanced in mouse fed beta pleated sheet-rich pro- teins. The local amyloid in joints of chicken and mammary corpora amylacea is discussed. Differences in glycosylation of glycoproteins among the APPs, as has been shown for α1-acid glycoprotein, have to be considered. More knowledge on the reactivity patterns may lead to implication of APPs in the diagnostics and staging of a disease. Calculation of an index from values of several acute phase variables increases the power of APPs in monitoring unhealthy individuals in animal populations. Vaccinations, just as infections in eliciting acute phase response seem to limit the profitability of vaccines because acute phase reactions are contraproductive in view of muscle anabolism. Interest is focused on amino acid patterns and vitamins in view of dietary nutrition effect on sick and convalescing animals. When inexpensive methodology such as liquid phase methods (nephelometry, turbidimetry) or protein array technology for rapid APPs measurement is available, APPs have a future in routine diagnostics. Specific groups of patients may be screened or populations monitored by using APPs.

关键词： Acute phase protein Amino acid Joint Mammary gland Mastitis Serum amyloid A (SAA) Vitamin A

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The Microflora of Gills, Gut and Skin of European Eels （Anguilla anguilla） in Lakes of Latvia

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Journal of Food Science and Engineering 2015年第3期5卷 130-136页

作者： Vita Strazdina Margarita Terentjeva Olga Valcina Inga Eizenberga Aleksandr Novoslavskij Jevgenija Osmjana Aivars Berzins Latvia Institute of Food Safety Animal Health and Environment "BIOR " Lejupes Street 3 Riga L V 1076 Latvia Institute of Food and Environmental Hygiene Faculty of Veterinary Medicine Latvia University of Agriculture Helmaga Street 8Jelgava L V 3004 Latvia lithuanian veterinary academy Lithuanian University of Health Sciences Til~ds Street 18 Kaunas 47181 Lithuania

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