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New generation of multi-scale NWP system (GRAPES):general scientific design

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Chinese Science Bulletin 2008年第22期53卷 3433-3445页

作者： CHEN DeHui XUE JiShan YANG XueSheng ZHANG HongLiang SHEN XueShun HU JiangLin WANG Yu JI liren CHEN JiaBin State Key laboratory of Server Weather Chinese Academy of Meteorological Sciences Beijing 100081 China National Meteorological Center Beijing 100081 China Institute of Atmospheric Physics Chinese Academy of Sciences Beijing 100089 China

A new generation of numerical prediction system GRAPES (a short form of Global/Regional Assimilation and PrEdiction System) was set up in China Meteorological Administration (CMA). This paper focuses on the scientific design and preliminary results of the numerical prediction model in GRAPES, including basic idea and strategy of the general scientific design, multi-scale dynamic core, physical package configuration, architecture and parallelization of the codes. A series of numerical experiments using the real data with horizontal resolutions from 10 to 280 km and idealized experiments with very high resolution up to 100 m are conducted, giving encouraging results supporting the multi-scale application of GRAPES. The results of operational implementation of GRAPES model in some NWP centers are also presented with stress at evaluations of the capability to predict the main features of precipitation in China. Finally the issues to be dealt with for further development are discussed.

关键词：数值天气预报气象学天气预测 GRAPES

Wintertime extreme precipitation event over southern China and its typical circulation features

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Chinese Science Bulletin 2014年第10期59卷 1036-1044页

作者： Haifeng Zong Cholaw Bueh liren Ji The International Center for Climate and Environment Sciences Institute of Atmospheric Physics Chinese Academy of Sciences State Key laboratory of Numerical Modeling for Atmospheric Sciences and Geophysical Fluid Dynamics Institute of Atmospheric Physics Chinese Academy of Sciences

This study objectively defined an extreme precipitation event(EPE)over southern China and investigated the associated typical circulation pattern on the intraseasonal time *** occurrence of the EPE resulted from the joint operations of anomalous circulations over the subtropics and mid-high *** the EPE,simultaneous enhancements of the trough over the Bay of Bengal(BBT)and the Western Pacific subtropical high(WPSH)facilitated the abundant water vapor conditions over southern China,whereas the weakened East Asian major trough(EAT)led to a moderate cold air invasion to that region,producing a persistent convergence zone over southern *** train pattern in North Atlantic and Europe may be viewed as precursory signals for the EPE over southern China,and it contributed to the Rossby wave propagation in association with the *** wave packets propagated toward East Asia mainly along the North African-Asian subtropical westerly jet ***-frequency disturbances along this waveguide contributed to the enhancement of the BBT and WPSH and the weakening of the EAT,constituting favorable circulation conditions for the EPE.

关键词： Extreme precipitation event Troughover the Bay of Bengal - Western Pacific subtropicalhigh Precursory signal

Progress in Electrolyte Engineering of Aqueous Batteries in a Wide Temperature Range

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Transactions of Tianjin University 2023年第5期29卷 321-346页

作者： Lingjun He Chuyuan Lin Peixun Xiong Hui Lin Wenbin Lai Jingran Zhang Fuyu Xiao liren Xiao Qingrong Qian Qinghua Chen Lingxing Zeng Engineering Research Center of Polymer Green Recycling of Ministry of Education Fujian Key Laboratory of Pollution Control and Resource ReuseCollege of Environmental and ResourcesFujian Normal UniversityFuzhou 350007China Inorganic Chemistry I Technische Universität DresdenBergstrasse 6601069 DresdenGermany College of Chemistry and Materials Science Fujian Normal UniversityFuzhou 350007China Key laboratory of Advanced Energy Materials Chemistry(Ministry of Education) College of ChemistryNankai UniversityTianjin 300071China

Aqueous rechargeable batteries are safe and environmentally friendly and can be made at a low cost;as such,they are attracting attention in the field of energy ***,the temperature sensitivity of aqueous batteries hinders their practical *** solvent water freezes at low temperatures,and there is a reduction in ionic conductivity,whereas it evaporates rapidly at high temperatures,which causes increased side *** review discusses recent progress in improving the performance of aqueous batteries,mainly with respect to electrolyte engineering and the associated strategies employed to achieve such improvements over a wide temperature *** review focuses on fi ve electrolyte engineer-ing(aqueous high-concentration electrolytes,organic electrolytes,quasi-solid/solid electrolytes,hybrid electrolytes,and eutectic electrolytes)and investigates the mechanisms involved in reducing the solidifi cation point and boiling point of the electrolyte and enhancing the extreme-temperature electrochemical ***,the prospect of further improving the wide temperature range performance of aqueous rechargeable batteries is presented.

关键词： Aqueous batteries Electrolyte engineering Wide temperature range Hydrogen bond

Enhanced genome editing to ameliorate a genetic metabolic liver disease through co-delivery of adeno-associated virus receptor

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Science China(Life Sciences) 2022年第4期65卷 718-730页

作者： Shuming Yin Lie Ma Tingting Shao Mei Zhang Yuting Guan liren Wang Yaqiang Hu Xi Chen Honghui Han Nan Shen Wenjuan Qiu Hongquan Geng Yongguo Yu Shichang Li Weishi Yu Mingyao Liu Dali Li Shanghai Key laboratory of Regulatory Biology Institute of Biomedical Sciences and School of Life SciencesEast China Normal UniversityShanghai200241China Bioray Laboratories Inc. Shanghai200241China Xinhua Hospital Shanghai Jiao Tong University School of MedicineShanghai200092China College of Physical Education and Health East China Normal UniversityShanghai200241China Key laboratory of Adolescent Health Assessment and Exercise Intervention Ministry of EducationCollege of Physical Education and HealthEast China Normal UniversityShanghai200241China CIPHER GENE LLC Beijing100089China

Genome editing through adeno-associated viral(AAV) vectors is a promising gene therapy strategy for various diseases,especially genetic disorders. However, homologous recombination(HR) efficiency is extremely low in adult animal models. We assumed that increasing AAV transduction efficiency could increase genome editing activity, especially HR efficiency, for in vivo gene therapy. Firstly, a mouse phenylketonuria(PKU) model carrying a pathogenic R408W mutation in phenylalanine hydroxylase(Pah) was generated. Through co-delivery of the general AAV receptor(AAVR), we found that AAVR could dramatically increase AAV transduction efficiency in vitro and in vivo. Furthermore, co-delivery of SaCas9/sgRNA/donor templates with AAVR via AAV8 vectors increased indel rate over 2-fold and HR rate over 15-fold for the correction of the single mutation in Pah;mice. Moreover, AAVR co-injection successfully increased the site-specific insertion rate of a 1.4 kb Pah cDNA by 11-fold, bringing the HR rate up to 7.3% without detectable global off-target effects. Insertion of Pah cDNA significantly decreased the Phe level and ameliorated PKU symptoms. This study demonstrates a novel strategy to dramatically increase AAV transduction which substantially enhanced in vivo genome editing efficiency in adult animal models, showing clinical potential for both conventional and genome editing-based gene therapy.

关键词： CRISPR/Cas9 gene therapy adeno-associated virus(AAV) AAVR Pah PKU

HIF-1α-induced expression of m6A reader YTHDF1 drives hypoxia-induced autophagy and malignancy of hepatocellular carcinoma by promoting ATG2A and ATG14 translation

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Signal Transduction and Targeted Therapy 2021年第3期6卷 988-1000页

作者： Qing Li Yong Ni liren Zhang Runqiu Jiang Jing Xu Hong Yang Yuanchang Hu Jiannan Qiu Liyong Pu Jinhai Tang Xuehao Wang Hepatobiliary Center The First Affiliated Hospital of Nanjing Medical UniversityKey Laboratory of Liver TransplantationChinese Academy of Medical SciencesNHC Key Laboratory of Living Donor Liver Transplantation(Nanjing Medical University)NanjingJiangsu ProvinceChina School of Medicine Southeast UniversityNanjingChina department of Hepatopancreatobiliary Surgery Shenzhen Second People's HospitalThe First Affiliated Hospital of Shenzhen UniversityShenzhenGuangdongChina Department of Hepatobiliary Surgery The Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjingJiangsu ProvincePeople's Republic of China Medical School of Nanjing University NanjingJiangsuChina Department of Oncology The First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu ProvinceChina Department of Immunology Key Laboratory of Immune Microenvironment and DiseaseNanjing Medical UniversityNanjingJiangsu ProvinceChina Department of General Surgery The First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu ProvinceChina

N6-methyladenosine(m6A),and its reader protein YTHDF1,play a pivotal role in human tumorigenesis by affecting nearly everystage of RNA *** activation is one of the ways by which cancer cells survive ***,the possibleinvolvement of m6A modification of mRNA in hypoxia-induced autophagy was unexplored in human hepatocellular carcinoma(HCO).In this study,specific variations in YTHDF1 expression were detected in YTHDF1-overexpressing,knockout,and-knockdownHCC cells,HCC organoids,and HCC patient-derived xenograft(PDX)murine ***1 expression and hypoxia inducedautophagy were significantly correlated in vitro;signifhcant overexpression of YTHDF1 in HCC tissues was associated with poorprognosis,Multivariate cox regression analysis identihed YTHDF1 expression as an independent prognostic factor in patients *** HC models conhrmed that YTHDF1 deficiency inhibited HCC autophagy,growth,and *** reporterassays and chromatin immunoprecipitation demonstrated that HlIF-1a regulated YTHDF1 transcription by directly binding to itspromoter region under *** results of methylated RNA immunoprecipitation sequencing,proteomics,and polysomeprofling indicated that YTHDF1 contibuted to the translation of autophagy-related genes ATG2A and ATG14 by binding to m6A-modifhed ATG2A and ATG14 mRNA,thus facilitating autophagy and autophagy-related malignancy of *** together,HlE-1d-induced YTHDF1 expression was associated with hypoxia-induced autophagy and autophagy-related HCC progression via promoting translation of autophagy-related genes ATG2A and ATG14 in a m6A-dependent *** fndings suggest thatYTHDF1 is a potential prognostic biomarker and therapeutic target for patients with HCC.

关键词： malignancy hepatocellular inhibited

Gene editing and its applications in biomedicine

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Science China(Life Sciences) 2022年第4期65卷 660-700页

作者： Guanglei Li Xiangyang Li Songkuan Zhuang liren Wang Yifan Zhu Yangcan Chen Wen Sun Zeguang Wu Zhuo Zhou Jia Chen Xingxu Huang Jin Wang Dali Li Wei Li Haoyi Wang Wensheng Wei School of Life Science and Technology ShanghaiTech UniversityShanghai201210China Department of Clinical laboratory Shenzhen Institute of Translational MedicineThe First Affiliated Hospital of Shenzhen UniversityShenzhen Second People’s HospitalShenzhen518035China Shanghai Frontiers Science Research Base of Genome Editing and Cell Therapy Shanghai Key Laboratory of Regulatory BiologySchool of Life SciencesEast China Normal UniversityShanghai200241China State Key laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing100101China University of Chinese Academy of Sciences Beijing100049China Institute for Stem Cell and Regenerative Medicine Chinese Academy of SciencesBeijing100101China Biomedical Pioneering Innovation Center Beijing Advanced Innovation Center for GenomicsPeking-Tsinghua Center for Life SciencesPeking University Genome Editing Research CenterState Key Laboratory of Protein and Plant Gene ResearchSchool of Life SciencesPeking UniversityBeijing100871China Gene Editing Center School of Life Science and TechnologyShanghaiTech UniversityShanghai201210China Bejing Institute for Stem Cell and Regenerative Medicine Beijing100101China HIT Center for Life Sciences Harbin Institute of TechnologyHarbin150001China

The steady progress in genome editing, especially genome editing based on the use of clustered regularly interspaced short palindromic repeats(CRISPR) and programmable nucleases to make precise modifications to genetic material, has provided enormous opportunities to advance biomedical research and promote human health. The application of these technologies in basic biomedical research has yielded significant advances in identifying and studying key molecular targets relevant to human diseases and their treatment. The clinical translation of genome editing techniques offers unprecedented biomedical engineering capabilities in the diagnosis, prevention, and treatment of disease or disability. Here, we provide a general summary of emerging biomedical applications of genome editing, including open challenges. We also summarize the tools of genome editing and the insights derived from their applications, hoping to accelerate new discoveries and therapies in biomedicine.

关键词： gene editing CRISPR high-throughput functional genomics diagnostics animal model therapeutics

A roughness parameter considering joint material properties and peak shear strength model for rock joints

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International Journal of Mining Science and Technology 2021年第3期31卷 413-420页

作者： liren Ban Weisheng Du Tianwei Jin Chengzhi Qi Xiaozhao Li School of Civil and Transportation Engineering Beijing University of Civil Engineering and ArchitectureBeijing 100044China Deep Mining and Rock Burst Research Institute China Coal Research InstituteBeijing 100013China State Key laboratory of Coal Mining and Clean Utilization China Coal Research InstituteBeijing 100013China

This study aims at proposing a reasonable roughness parameter that can reflect the peak shear strength(PSS)of rock ***,the contribution of the asperities with different apparent dip angles to shear strength is *** the shear strength of the entire joint asperities is *** results showed that the PSS of the entire joint asperities is proportional to a key parameter hs,which is related to the geometric character of the joint surface and the joint material *** parameter hsis taken as the new roughness parameter,and it is reasonable to associate the PSS with the geometric characteristics of the joint *** on the new roughness parameter and shear test results of 20 sets of joint specimens,a new PSS model for rock joints is *** new model is validated with the artificial joints in this paper and real rock joints in published *** showed that it is suitable for different types of rock joints except for gneiss *** new model has the form of the Mohr-Coulomb model,which can directly reflect the relationship between the 3 D roughness parameters and the peak dilation angle.

关键词： Roughness Joint material properties Rock joints Peak shear strength Asperities

ADS1 mediates brassinosteroids-induced auxin biosynthesis in Arabidopsis thaliana

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ADS1 mediates brassinosteroids-induced auxin biosynthesis in...

Efficient photoactivatable Dre recombinase for cell type specific spatiotemporal control of genome engineering in the mouse

2018全国植物生物学大会

作者： Hongyong Shi Zengxiu Feng liren Du Jingjie Zhang Dongzhi Zhang Jia Li Ministry of Education Key laboratory of Cell Activities and Stress Adaptations School of Life SciencesLanzhou University

A genome-wide activation tagging screen for genetic modifers of det2-1,a brassinosteroid(BR)-deficient mutant,led to the identification of adsl-D,a dominant suppressor of det2-l.A det 2-1 ads1-Ddouble mutant shows a significantly elongated leaf phenotype compared to *** ads1-D single mutant shows elongated leaf petiole,bend-down leaf tip and elongated hypocotyl relative to wild type *** analyses also indicated that ads1-Dcan partially suppress the defective phenotypes ofbri1-9 and *** expression patterns of ADS1 are similar to those of *** two-hybrid,bimoleculer fluorescence complementation(BIFC),coimmunoprecipitation,and pull-down assays demonstrated that ADS1 can physically interact with BIN2 and its closest homologs,BILl and *** vitro phosphorylation assay indicated that ADS1 can be phosphorylated by *** ads1-D can rescue weak BR signaling mutants,it cannot rescue the strong mutantbril-701 brllbrl3,suggesting that the function of ADS 1 depends on functional BR ***1 expression levels were not significantly alteredeither after BL(the most active form of BRs) treatment or in a BR-deficient mutant,suggesting that ADS1 may not be transcriptionally regulated by *** addition,our data indicated that BL can induce the accumulation of ADS1 in the *** ADS1-OXseedlings showed a phenotype similar to that of auxin overproducing mutants,whereasadsl-Cas9 exhibits a phenotype which is reminiscent of auxindeficient ***,ADS1 may be genetically related to an auxin signaling or biosynthetic *** the differentially expressed genes(DEGs) in ADS1-OXwith auxin-induced DEGs revealed that 466 of 835(55%) genes in ADS1-OX were co-regulated by *** biosynthetic genes,YUC3 and YUC7,were significantly upregulated in ADS1-OXand downregulated in ***,YUC3 and YUC7 were significantly up-regulated in BRI1-OX and bin2-3 billbil2 and down-regulated in bri1-701 *** addition,BL trea

关键词： Brassinosteroids ADS1 BIN2 Auxin YUCCA3 YUCCA7

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Efficient photoactivatable Dre recombinase for cell type spe...