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Engineered extracellular vesicles efficiently deliver CRISPR-Cas9 ribonucleoprotein(RNP)to inhibit herpes simplex virus1 infection in vitro and in vivo

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Acta Pharmaceutica Sinica B 2024年第3期14卷 1362-1379页

作者： Yuanda Wan liren Li Ruilin Chen Jiajia Han Qiyun Lei Zhipeng Chen Xiaodong Tang Wenyu Wu Shuwen Liu Xingang Yao NMPA Key laboratory for Research and Evaluation of Drug Metabolism Guangdong Provincial Key Laboratory of New Drug ScreeningSchool of Pharmaceutical SciencesSouthern Medical UniversityGuangzhou 510515China Center of Clinical Pharmacy Nanfang HospitalSouthern Medical UniversityGuangzhou 510515China State Key laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangzhou 510060China Key laboratory of Infectious Diseases Research in South China(Southern Medical University) Ministry of EducationGuangzhou 510515China

Extracellular vesicles(EVs)have recently emerged as a promising delivery platform for CRISPR/Cas9 ribonucleoproteins(RNPs),owing to their ability to minimize off-target effects and immune ***,enhancements are required to boost the efficiency and safety of Cas9 RNP enrichment within *** response,we employed the Fc/Spa interaction system,in which the human Fc domain was fused to the intracellular domain of PTGFRN-Δ687 and anchored to the EV ***,the B domain of the Spa protein was fused to the C domain of cargos such as Cre or *** to the robust interaction between Fc and Spa,this method enriched nearly twice the amount of cargo within the *** loaded with spCas9 RNP targeting the HSV1 genome exhibited significant inhibition of viral replication in vitro and in ***,following neuron-targeting peptide RVG modification,the in vivo dosage in neural tissues substantially increased,contributing to the clearance of the HSV1 virus in neural tissues and exhibiting a lower off-target *** findings establish a robust platform for efficient EV-based SpCas9 delivery,offering potential therapeutic advantages for HSV1 infections and other neurological disorders.

关键词： Extracellular vesicles CRISPR/Cas9 Ribonucleoproteins PTGFRN Fc/Spa HSV1 Neuron-targeting Delivery

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Oil Pocket's Bearing Capacity Analysis of Liquid Hydrostatic Worktable in Gantry Moving Milling Center

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Chinese Journal of Mechanical Engineering 2014年第5期27卷 1008-1017页

作者： ZHAO Jianhua LIANG Yingna GAO Dianrong Hebei Provincial Key laboratory of Heavy Machinery Fluid Power Transmission and Control Yanshan University liren College Yanshan University

Durning the design process of hydrostatic rotary worktable,the processing and assembly tolerance,（the offset of worktable and the gap of the oil film’s thickness）is *** it will cause that the real bearing of oil pocket deviates from the initial design value,and then the performance of rotary worktable will be reduced *** to now,no effort is found toward the research of influence of the processing and assembly tolerance on the performance of the rotary *** the hydrostatic oil film is assumed as the elastomer in this paper,and then the bearing capacity of the oil pocket is studied with and without the mass offset of the worktable by taking an expression between the bearing capacity and the oil film’s thickness of the oil pocket as the deform compatibility *** influence of the processing tolerance of the oil sealing belt’s gap on the bearing capacity of the oil pocket is *** the light of the liquid hydrostatic worktable of Gantry Moving Milling Center using on the scene,the oil pocket’s pressure of the worktable is tested using Rotary Worktable Test System under the circumstance of the mass offset of the worktable and the gap tolerance of the oil sealing belt,and then the equivalent offset of worktable,the average pressure of the oil pocket and the actual thickness of the oil film are analyzed *** test results show that the bearing capacity component of the oil pocket caused by G is consistent,and the component caused by M is relative to the position of the oil *** the oil sealing belt’s gap is larger than the theoretical value,the bearing capacity of the oil pocket is smaller than the others;whereas the bearing capacity of the oil pocket is larger than the *** maximum and minimum equivalent offsets are 0.256 4 mm and 0.047 5 mm,respectively,and the average oil pocket pressure varies from 0.345 MPa to 0.460 MPa,the maximum and minimum value of the actual oil film thickness are 109.976？m（No.7 oil

关键词： Gantry Moving Milling Center bearing capacity processing and assembly tolerance mass offset

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Regulation of swelling-activated chloride channels in embryonic chick heart cells

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Cell Research 2003年第1期13卷 21-28页

作者： HUA WEI, VAN AI MEI, JIA TING SUN, HAN QING ZHOU, ZHI HONG ZHANGDepartment of Physiology and Biophysics, liren laboratory, School of Life Sciences, Fudan University, Shanghai 200433, China Fishery College, Shanghai Fisheries University, Shanghai 200090, China 2Fishery College Shanghai Fisheries University Shanghai 200090 China 1Department of Physiology and Biophysics Liren Laboratory School of Life Sciences Fudan University Shanghai 200433 China 1Department of Physiology and Biophysics Liren Laboratory School of Life Sciences Fudan University Shanghai 200433 China 1Department of Physiology and Biophysics Liren Laboratory School of Life Sciences Fudan University Shanghai 200433 China 1Department of Physiology and Biophysics Liren Laboratory School of Life Sciences Fudan University Shanghai 200433 China

Swelling-activated Cl- currents, I(ci,swell), were measured during hyposmotic shock in white Leghorn embryonic chick heart cells using the whole-cell recording of patch-clamp technique. Genistein, an inhibitor of protein tyrosine kinase (PTK), suppressed I(ci,swell), Under isosmotic condition phorbol 12-myristate 13-acetate (PMA), an activator of PKC, elicited the Cl~ current similar to that in hyposmotic solution, whereas hyposmotic shock did not elicit I(ci,swell) in chelerythrine chloride(an inhibitor of PKC)-treated cells. Con-focal microscopy experiments using FITC-phalloidin as a fluorescent label of F-actin showed that the actin network was moved from cortical region of the cell to the center after hyposmotic shock as compared with the image under isosmotic condition. When the cells were treated with cytochalasin B (CB) or cytochalasin D (CD) under isosmotic condition the disruption of the F-actin integrity was observed, and I(ci,Sweii) was not elicited. With combination treatment of CB with PMA,

关键词： swelling activation myocardium chloride current F-actin phosphorylation.

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A general method for large-scale fabrication of Cu nanoislands/dragonfly wing SERS flexible substrates

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Chinese Physics B 2018年第1期27卷 564-569页

作者：王玉红王明利沈琳朱艳英孙鑫史国超许晓娜李瑞峰马万里 Key laboratory for Microstructural Material Physics of Hebei Province School of Science Yanshan University liren College of Yanshan University Department of Mathematics NC State University

Noble metal nanorough surfaces that support strong surface-enhanced Raman scattering （SERS） is widely applied in the practical detection of organic molecules. A low-cost, large-area, and environment-friendly SERS-active substrate was acquired by sputtering inexpensive copper （Cu） on natural dragonfly wing （DW） with an easily controlled way of magnetron sputtering. By controlling the sputtering time of the fabrication of Cu on the DW, the performance of the SERS substrates was greatly improved. The SERS-active substrates, obtained at the optimal sputtering time （50 min）, showed a low detection limit （10-6M ） to 4-aminothiophenol （4-ATP）, a high average enhancement factor （EF, 1.98 x10^4）, excellent signal uniformity, and good reproducibility. In addition, the results of the 3D finite-difference time-domain （3D- FDTD） simulation illustrated that the SERS-active substrates provided high-density ＂hot spots＂, leading to a large SERS enhancement.

关键词： surface-enhanced Raman scattering dragonfly wing copper magnetron sputtering

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Influence of Non-smooth Surface on Tribological Properties of Glass Fiber-epoxy Resin Composite Sliding against Stainless Steel under Natural Seawater Lubrication

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Chinese Journal of Mechanical Engineering 2015年第6期28卷 1171-1176页

作者： WU Shaofeng GAO Dianrong LIANG Yingna CHEN Bo School of Mechanical Engineering Yanshan University Heibei Provincial Key laboratory of Heavy Machinery Fluid Power Transmission and Control Yanshan University liren College Yanshan University

With the development of bionics, the bionic non-smooth surfaces are introduced to the field of tribology. Although non-smooth surface has been studied widely, the studies of non-smooth surface under the natural seawater lubrication are still very fewer, especially experimental research. The influences of smooth and non-smooth surface on the frictional properties of the glass fiber-epoxy resin composite（GF/EPR） coupled with stainless steel 316 L are investigated under natural seawater lubrication in this paper. The tested non-smooth surfaces include the surfaces with semi-spherical pits, the conical pits, the cone-cylinder combined pits, the cylindrical pits and through holes. The friction and wear tests are performed using a ring-on-disc test rig under 60 N load and 1000 r/min rotational speed. The tests results show that GF/EPR with bionic non-smooth surface has quite lower friction coefficient and better wear resistance than GF/EPR with smooth surface without pits. The average friction coefficient of GF/EPR with semi-spherical pits is 0.088, which shows the largest reduction is approximately 63.18% of GF/EPR with smooth surface. In addition, the wear debris on the worn surfaces of GF/EPR are observed by a confocal scanning laser microscope. It is shown that the primary wear mechanism is the abrasive wear. The research results provide some design parameters for non-smooth surface, and the experiment results can serve as a beneficial supplement to non-smooth surface study.

关键词： non-smooth surface friction coefficient wear resistance glass fiber-epoxy resin composite seawater lubrication stainless steel 316L

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The corrosion characteristic and mechanism of Mg-5Y-1.5Nd-xZn-0.5Zr(x=0,2,4,6 wt.%)alloys in marine atmospheric environment

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Journal of Magnesium and Alloys 2024年第1期12卷 139-158页

作者： Quantong Jiang Dongzhu Lu liren Cheng Nazhen Liu Baorong Hou CAS Key laboratory of Marine Environmental Corrosion and Bio-fouling Institute of OceanologyChinese Academy of SciencesNo.7 Nanhai RoadQingdao 266071PR China State Key laboratory of Rare Earth Resource Utilization Changchun Institute of Applied ChemistryChinese Academy of SciencesNo.5625 Renmin StreetChangchun 130022PR China Open Studio for Marine Corrosion and Protection Pilot National Laboratory for Marine Science and Technology(Qingdao)No.1 Wenhai RoadQingdao 266071PR China Sanya Institute of Oceanology South China Sea Institute of OceanologyChinese Academy of SciencesZhenzhou RoadSanya 572000PR China

The microstructure and precipitated phases of as-cast Mg-5Y-1.5Nd-x Zn-0.5Zr(x=0,2,4,6 wt.%)alloys were investigated by optical microscopy,scanning electron microscopy,energy-dispersive spectrometry and X-ray *** exposure corrosion experiment of these magnesium alloys was tested in South China Sea and KEXUE vessel atmospheric *** corrosion characteristic and mechanism of magnesium alloys of Mg-5Y-1.5Nd-x Zn-0.5Zr(x=0,2,4,6 wt.%)alloys were analyzed by weight loss rate,corrosion depth,corrosion products and corrosion *** electrochemical corrosion tests were also measured in the natural *** comprehensive results showed that Mg-5Y-1.5Nd-4Zn-0.5Zr magnesium alloy existed the best corrosion resistance whether in the marine atmospheric environment and natural seawater *** depended on the microstructure,type and distribution of precipitated phases in Mg-5Y-1.5Nd-4Zn-0.5Zr magnesium *** quantity anodic precipitated phases in the microstructure of Mg-5Y-1.5Nd-4Zn-0.5Zr alloy played the key role in the corrosion resistance.

关键词： Marine atmospheric environment Exposure corrosion Magnesium alloy Corrosion rate Corrosion mechanism

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Friction and Wear Study on Friction Pairs with a Biomimetic Non-smooth Surface of 316L Relative to CF/PEEK under a Seawater Lubricated Condition

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Chinese Journal of Mechanical Engineering 2019年第4期32卷 47-60页

作者： Yingna Liang Dianrong Gao Bo Chen Jianhua Zhao School of Mechanical Engineering Yanshan University Qinhuangdao 066004China liren College Yanshan University Qinhuangdao 066004 China Heibei Provincial Key laboratory of Heavy Machinery Fluid Power Transmission and Control Yanshan University Qinhuangdao 066004 China

Current studies of a seawater axial piston pump mainly solve the problems of corrosion and wear in a slipper pair by selecting materials with corrosion resistance, self-lubrication, and wear resistance. In addition, an appropriate biomimetic non-smooth surface design for the slipper pair can further improve the tribological behavior. In this paper, 316 L stainless steel and CF/PEEK were selected to process the upper and bottom specimens, and the biomimetic non-smooth surface was introduced into the interface between the friction pair. The friction and wear tests were performed on a MMD-5 A tester at a rotation speed of 1000 r/min and load of 200 N under seawater lubricated condition. The results indicate that the main friction form of the smooth surface friction pair corresponds to abrasive wear and adhesive wear and that it exhibits a friction coe cient of 0.05–0.07, a specimen temperature of 56 ℃, a high wear rate, and surface roughness. Pits on the non-smooth surface friction pairs produced hydrodynamic lubrication and reduced abrasive wear, and thus the plowing e ect is their main friction form. The non-smooth surface friction pairs exhibit a friction coe cient of 0.03–0.04, a specimen temperature of 48 ℃, a low wear rate, and surface roughness. The study has important theoretical significance for enriching the lubrication, friction, and wear theory of a seawater axial piston pump, and economic significance and military significance for promoting the marine development and the national defense military.

关键词： Biomimetic non-smooth surface 316L CF/PEEK Seawater lubrication Friction and wear

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Experimental Study on Influence of Dimples on Lubrication Performance of Glass Fiber-epoxy Resin Composite under Natural Seawater Lubrication

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Chinese Journal of Mechanical Engineering 2017年第1期30卷 110-117页

作者： WU Shaofeng GAO Dianrong LIANG Yingna CHEN Bo School of Mechanical Engineering Yanshan UniversityQinhuangdao 066004China Heibei Provincial Key laboratory of Heavy Machinery Fluid Power Transmission and Control Yanshan UniversityQinhuangdao 066004China liren College Yanshan UniversityQinhuangdao 066004China

Bionic non-smooth surface is widely applied in metal and ceramics materials. In order to introduce this technology to high pressure seawater pump, the influence of bionic non-smooth surface on the engineering plastics used in pump should be investigated. The comparative tests are carried out with a ring-on-disc configuration under 800, 1000, 1200 and 1400 r/min in order to research the influence of the bionic non-smooth surface on glass fiber-epoxy resin composite（GF/EPR） under natural seawater lubrication. The disc surfaces are textured with five kinds of pits, which are semi-spherical, conical, cone-cylinder combined, cylindrical pits and through holes, respectively. A smooth surface is tested as reference. The results show that the lubrication performance of dimpled GF/EPR sample is much better than that of the smooth sample under all rotational speeds. The semi-spherical pits surface has more obvious friction reduction than the others, which shows that the least reduction is approximately 43.29% of smooth surface under 1200 r/rain. However, the wear level is only marginally influenced by dimples. The surface morphology investigations disclose severe modifications caused by abrasive wear primarily. The results are helpful to vary friction properties of GF/EPR by non-smooth surface, or provide references to the design of non-smooth surfaces under certain condition.

关键词： !bionic non-smooth surface seawater lubrication friction coefficient glass fiber-epoxy resin composite stainless steel 316L

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Actuation Spaces Synthesis of Lower-Mobility Parallel Mechanisms Based on Screw Theory

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Chinese Journal of Mechanical Engineering 2021年第1期34卷 280-291页

作者： Shihua Li Yanxia Shan Jingjun Yu Yaxiong Ke Hebei Provincial Key laboratory of Parallel Robot and Mechatronic Systems Yanshan UniversityQinhuangdao 066004China School of Mechanical Engineering Yanshan UniversityQinhuangdao 066004China School of liren Yanshan UniversityQinhuangdao 066004China Robotics Institute Beihang UniversityBeijing 100191China

The lower-mobility parallel mechanism has been widely used in the engineering field due to its numerous excellent ***,little work has been devoted to the actuator selection and placement that best satisfy the system's functional requirements during concept *** this study,a unified approach for synthesizing the actuation spaces of both rigid and flexure parallel mechanisms has been presented,and all possible combinations of inputs could be obtained,laying a theoretical foundation for the subsequent optimization of *** to the linear independence of actuation space and constraint space of the lower-mobility parallel mechanism,a general expression of actuation spaces in the format of screw systems is deduced,a unified synthesis process for the lower-mobility parallel mechanism is derived,and the efficiency of the method is validated with two selective examples based on screw *** study presents a theoretical framework for the input selection problems of parallel mechanisms,aiming to help designers select and place actuators in a correct and even optimal way after the configuration design.

关键词： Lower-mobility parallel mechanism Screw theory Actuation space Actuator placement Input selection

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Talbot effect in anti-PT symmetric synthetic photonic lattices

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Chinese Optics Letters 2024年第3期22卷 107-111页

作者：刘兆峰唐思维温增润高垣梅蔡阳健郑立仁 Shandong Provincial Engineering and Technical Center of Light Manipulations&Shandong Provincial Key laboratory of Optics and Photonic Device School of Physics and ElectronicsShandong Normal UniversityJinan 250358China School of Physical Science and Technology Soochow UniversitySuzhou 215006China

We investigated the Talbot effect in an anti-parity-time(PT)symmetric synthetic photonic lattice composed of two coupled fiber *** calculated the band structures and found that with an increase in the gain-loss parameter,the band transitions from a real spectrum to a complex *** study the influence of phase in the Hermitian operator on the Talbot effect,and the Talbot effect disappears when the period of the input field is N>*** study shows that the variation of Talbot distance can also be modulated by non-Hermitian coefficients of gain and *** work may find significant applications in pulse repetition-rate multiplication,temporal invisibility,and tunable intensity amplifiers.

关键词： Talbot effect synthetic dimensions anti-PT symmetry photonic lattices

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