检索结果-南通市图书馆

A PTEN translational isoform has PTEN-like activity

Chinese Journal of Cancer Research 2015年第5期27卷 524-532页

作者： Xie Zhang Bowei Yin Fangfang Zhu Guochang Huang Hong Li Department of Gastroenterology Ningbo Medical CenterLiHuiLi Hospital School of Medicine Ningbo University ningbo Medical Center Ningbo Medical CenterLiHuiLi hospital Minimally Invasive Abdominal Surgery Ningbo Medical CenterLiHuiLi hospital

Background： To identify PTEN isoform and explore its potential role in tumor suppression. Methods： Western blotting, over-expression, shRNA mediated knocking-down, and bioinformatic analysis were used to identify PTEN isoform and test its effect on PI3K-Akt signaling pathway. Cell proliferation, apoptosis, and migration assays were used to test PTEN isoform＇s biological activities. Results： The PTEN isoform is about 15 kDa bigger than PTEN and its expression is dependent on PTEN status. Immunoprecipitation for PTEN isoform followed by screening with antibodies against ISG15, SUMO1/2/3, Ubiquitin, and Nedd8 showed the identified PTEN isoform is not a general proteinaceous post-translational modification. In addition, overexpression of PTEN cDNA in cells did not generate PTEN isoform whereas knocking-down of PTEN reduced the protein levels of both PTEN and PTEN isoform in a proportional manner. Analysis of PTEN DNA sequence disclosed an alternative translational starting code （CTG） upstream of canonical PTEN coding sequence. Expression of cloned PTEN isoform generated a protein with a size about 15 kDa bigger than PTEN and suppressed PI3K-Akt signaling pathway in cells. Overexpression of PTEN isoform also led to decrease in cell growth and enhanced serum starvation--and UV irradiation--induced apoptosis through activation of Caspase 3. Finally, expression of PTEN isoform inhibited cell migration in scratch assay. Conclusions： PTEN isoform has PTEN-Iike activity and might be a new tumor suppressor.

关键词： PTEN PTEN isoform rumor suppressor PI3K-Akt cell growth apoptosis cell migration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Vitamin D 1,25-Dihydroxyvitamin D_(3) reduces lipid accumulation in hepatocytes by inhibiting M1 macrophage polarization

引用

World Journal of Gastrointestinal Oncology 2024年第12期16卷 4685-4699页

作者： Wen-Jing Luo Xian-Wen Dong Hua Ye Qiao-Su Zhao Qiu-Bo Zhang Wen-Ying Guo Hui-Wei Liu Feng Xu Department of Gastroenterology Ningbo Medical Center Lihuili HospitalNingbo 315000Zhejiang ProvinceChina

BACKGROUND Non-alcoholic fatty liver disease(NAFLD),which is a significant liver condition associated with metabolic syndrome,is the leading cause of liver diseases globally and its prevalence is on the rise in most *** protective impact of vitamin D on NAFLD and its specific mechanism remains *** To examine the role of vitamin D in NAFLD and how vitamin D affects the polarization of hepatic macrophages in NAFLD through the vitamin D receptor(VDR)-peroxisome proliferator activated receptor(PPAR)γ*** Wild-type C57BL/6 mice were provided with a high-fat diet to trigger NAFLD model and administered 1,25-dihydroxy-vitamin D[1,25(OH)_(2)D_(3)]supplementation.1,25(OH)_(2)D_(3) was given to RAW264.7 macrophages that had been treated with lipid,and a co-culture with AML12 hepatocytes was set *** accumulation,lipid metabolism enzymes,M1/M2 phenotype markers,proinflammatory cytokines and VDR-PPARγpathway were *** Supplementation with 1,25(OH)_(2)D_(3) relieved hepatic steatosis and decreased the proinflammatory M1 polarization of hepatic macrophages in *** of 1,25(OH)_(2)D_(3) suppressed the proinflammatory M1 polarization of macrophages induced by fatty acids,thereby directly relieving lipid accumulation and metabolism in *** VDR-PPARγpathway had a notable impact on reversing lipid-induced proinflammatory M1 polarization of macrophages regulated by the administration of 1,25(OH)_(2)D_(3).CONCLUSION Supplementation with 1,25(OH)_(2)D_(3) improved hepatic steatosis and lipid metabolism in NAFLD,linked to its capacity to reverse the proinflammatory M1 polarization of hepatic macrophages,partially by regulating the VDRPPARγ*** involvement of 1,25(OH)_(2)D_(3) in inhibiting fatty-acid-induced proinflammatory M1 polarization of macrophages played a direct role in relieving lipid accumulation and metabolism in hepatocytes.

关键词： Non-alcoholic fatty liver disease Hepatocytes Macrophages Polarization Vitamin D receptor Peroxisome proliferator activated receptorγ

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Comparison on Clinical Characteristics of Multifocal Choroiditis and Punctate Inner Choroidopathy

引用

Eye Science 2011年第3期26卷 161-165页

作者： Zhaomeng Shen linyi Zhang Zhiguo Li Xian Zhang Hongbo Cui lihuili hospital of ningbo Ningbo 315040 China

Purpose:To compare the differences in the clinical characteristics of multifocal choroiditis(MFC) and punctate inner choriodopathy(PIC).Methods:A cross-sectional study,consisting of 22 cases(37 eyes) with MFC and 11 cases(18 eyes) with PIC,was carried *** indexes were measured and analysed to compare the differences in clinical characteristics of the subjects between two groups,including BCVA,avergae age,sex composition,presence of intraocular inflammation,choroidal neovascularization(CNV),and intraocular inflammation complications,such as cataract,cystoid macular edema(CME) and epiretinal membrane(ERM).Results:The avergae age of the MFC and PIC patients were(48.1±13.9) years and(32.1±10.2) years with a significant difference(P=0.043).CNV occurred more frequently in patients with PIC(PIC,55.6%;MFC,21.6%;P=0.016) compared with those with *** patients had a higher frequency of intraocular inflammation complications,such as,cataract(27.0%),CME(35.1%),ERM(5.4%).PIC patients presented none of these *** subjects presented with more visual impairments than PIC *** 51.4% of MFC patients had visual impairments and 22.2% of PIC patients had BCVA < 0.3(P=0.047).Conclusion:Both MFC and PIC are categorized into the umbrella term of "white spot syndromes".Although sharing similar ocular expressions,they present with different clinical *** with PIC had a higher frequency of CNV but a lower frequency of structural complications caused by intraocular inflammation and lower frequency of visual impairments compared with MFC counterparts.

关键词：临床特点脉络膜内蒙古特征和炎症反应视力障碍 MFC PIC

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Down-regulation of eIF5A-2 prevents epithelial-mesenchymal transition in non-small-cell lung cancer cells

引用

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第6期14卷 460-467页

作者： Guo-dong XU Xin-bao SHI Le-bo SUN Qing-yun ZHOU Da-wei ZHENG Huo-shun SHI Yong-liang CHE Zi-shan WANG Guo-feng SHAO Department of Thoracic & Cardiovascular Surgery Lihuili HospitalNingbo Medical CenterAffiliated Hospital of Medical SchoolNingbo University Department of Thoracic & Cardiovascular Surgery Ningbo First HospitalAffiliated Hospital of Medical SchoolNingbo University

Background:Epithelial-mesenchymal transition(EMT) is believed to be the critical process in malignant tumor invasion and metastases,and has a great influence on improving the survival rate in non-small-cell lung cancer(NSCLC) *** studies suggested that eukaryotic initiation factor 5A-2(eIF5A-2) might serve as an adverse prognostic marker of *** detected eIF5A-2 in NSCLC A549 cells,and found that the invasive capability correlates with the eIF5A-2 ***:Transforming growth factor(TGF)-β1 was used to induce EMT in A549 *** blotting,immunofluorescence,wound healing assay,and transwell-matrigel invasion chambers were used to identify phenotype *** blotting was also used to observe changes of the expression of *** down-regulated the eIF5A-2 expression using an eIF5A-2 siRNA and identified the phenotype changes by western blotting and *** tested the change of migration and invasion capabilities of A549 cells by the wound healing assay and transwell-matrigel invasion ***:After stimulating with TGF-β1,almost all A549 cells changed to the mesenchymal phenotype and acquired more migration and invasion *** cells also had higher eIF5A-2 protein ***-regulation of eIF5A-2 expression with eIF5A-2 siRNA transfection could change the cells from mesenchymal to epithelial phenotype and decrease tumor cell migration and invasive capabilities ***:The expression of eIF5A-2 was up-regulated following EMT phenotype changes in A549 cells,which correlated with enhanced tumor invasion and metastatic ***,in the A549 cell line,the process of EMT phenotype change could be reversed by eIF5A-2 siRNA,with a consequent weakening of both invasive and metastatic capabilities.

关键词： Non-small-cell lung cancer(NSCLC) Epithelial-mesenchymal transition(EMT) Eukaryotic initiation factor 5A-2(eIF5A-2) Transforming growth factor(TGF)-β1 A549

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Gallbladder neuroendocrine carcinoma diagnosis, treatment and prognosis based on the SEER database: A literature review

引用

World Journal of Clinical Cases 2022年第23期10卷 8212-8223页

作者： Xing-Chen Cai Sheng-Dong Wu Department of Hepato-Pancreato-Billiary Surgery The affiliated Lihuili HospitalNingbo UniversityNingboZhejiangChina

BACKGROUND Gallbladder neuroendocrine carcinoma(GB-NEC)has a low incidence rate;therefore,its clinical characteristics,diagnosis,treatment and prognosis are not well *** To review recent research and analyze corresponding data in the Surveillance Epidemiology and End Results(SEER)*** Data of GB-NEC(n=287)and gallbladder adenocarcinoma(GB-ADC)(n=19484)patients from 1975 to 2016 were extracted from the SEER *** analysis was performed using Kaplan–Meier and Cox proportional hazards regression.P<0.05 was considered statistically *** also reviewed 108 studies retrieved from PubMed and Reference Citation Analysis(https://***/).The keywords used for the search were:"(Carcinoma,Neuroendocrine)AND(Gallbladder Neoplasms)".RESULTS The GB-NEC incidence rate was 1.6%(of all gallbladder carcinomas),male to female ratio was 1:2 and the median survival time was 7 *** 1-,2-,3-and 5-year overall survival(OS)was 36.6%,17.8%,13.2%and 7.3%*** chromogranin A levels may be a specific tumor marker for the diagnosis of *** carcinoembryonic antigen,carbohydrate antigen(CA)-19-9 and CA-125 levels were associated with poor ***[hazard ratio(HR)=1.027,95%confidence interval(CI):1.006–1.047,P=0.01]and liver metastasis(HR=3.055,95%CI:1.839–5.075,P<0.001)are independent prognostic risk factors for *** with advanced GB-NEC treated with surgical resection combined with radiotherapy and/or chemotherapy may have a better prognosis than those treated with surgical resection *** was no significant difference in OS between GB-NEC and *** The clinical manifestations and prognosis of GB-NEC are similar to GB-ADC,but the treatment is completely *** diagnosis and treatment are the top priorities.

关键词： Clinical features Diagnosis Gallbladder neuroendocrine tumor Pathology Treatment

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Efficacy of epalrestat plus α-lipoic acid combination therapy versus monotherapy in patients with diabetic peripheral neuropathy: a meta-analysis of 20 randomized controlled trials

引用

Neural Regeneration Research 2018年第6期13卷 1087-1095页

作者： Ming Zhao Jia-Yi Chen Yu-Dong Chu Ya-Bin Zhu Lin Luo Shi-Zhong Bu Runliang Diabetes Laboratory Diabetes Research Center Ningbo University Department of Public Health Longsai Hospital Department of Nephrology Ningbo Medical Center Lihuili Eastern Hospital Medical School Ningbo University

OBJECTIVE： To evaluate the efficacy of α-lipoic acid（ALA） plus epalrestat combination therapy in the treatment of diabetic peripheral neuropathy（DPN）. DATA SOURCES： The electronic databases of Pub Med, Medline, Embase, the Cochrane Library, the Chinese National Knowledge Infrastructure, the Wanfang Database and the Chinese Biomedical Database were used to retrieve relevant studies without language restrictions. The search was conducted from the inception of each database to 7 October 2016. The key terms were（diabetic peripheral neuropathy or diabetic neuropathy or DPN） AND（α-lipoic acid or lipoic acid or thioctic acid） AND epalrestat. DATA SELECTION： All of the eligible studies met the following inclusion criteria：（1） Randomized controlled trials that compared efficacy and safety of epalrestat plus ALA combination therapy versus epalrestat or ALA monotherapy in patients with DPN.（2） The minimum duration of treatment was 2 weeks.（3） The DPN patients were diagnosed using the World Health Organization standardized type 2 diabetes mellitus and DPN criteria.（4） Studies contained at least one measure that could reflect the efficacy of the drug and nerve conduction velocities. Studies in which the control group used epalrestat or ALA combined with other drugs were excluded. Statistical analyses were performed using STATA software for meta-analysis. OUTCOME MEASURES： The primary outcomes were the therapeutic efficacy, median motor nerve conduction velocity（MNCV）, median sensory nerve conduction velocity（SNCV）, peroneal MNCV and peroneal ***： Twenty studies with 1894 DPN patients were included, including 864 patients in the ALA plus epalrestat group, 473 in the ALA group and 557 in the epalrestat group. The efficacy of ALA plus epalrestat combination therapy was superior to ALA and epalrestat monotherapies（RR = 1.29, 95% CI： 1.21–1.38; RR = 1.43, 95% CI： 1.34–1.54, respectively）. ALA plus epalrestat combination therapy also significantly improved median MNCV（WMD = 5

关键词： nerve regeneration antioxidant aldose reductase inhibitor diabetic complication diabetes combination therapy nerve conduction velocity nerve electrophysiology peripheral nerve injury neural regeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Evaluation of prognostic markers in severe drug-induced liver disease

引用

World Journal of Gastroenterology 2007年第4期13卷 628-632页

作者： Bo Li Zhi Wang Jian-Jiang Fang Ci-Yi Xu Wei-Xing Chen Department of Emergency Lihuili Hospital of Ningbo Medical Center Ningbo 315041 Zhejiang Province China Department of Pediatrics Ningbo Women and Children's Hospital Ningbo 315012 Zhejiang Province China Department of ICU Lihuili Hospital of Ningbo MedicalCenter Ningbo 315041 Zhejiang Province China Department of Gastroenterology First AffiliatedHospital Zhejiang University School of Medicine Hangzhou 310003 Zhejiang Province China

AIM: To analyze the outcome of patients with severe drug-induced liver disease (DILD) associated with jaundice classified as hepatocellular, cholestatic or mixed liver injury and to evaluate the validity of Hy’s rule and the most important predictors for outcome. METHODS: The Adverse Drug Reaction Advisory Committee was set up in 1997 in our hospital to identify all suspicions of DILD following a structured prospective report form. Liver damage was divided into hepatocellular, cholestatic, and mixed types according to laboratory and histologic criteria when available. Further evaluation of causality assessment was performed. RESULTS: From January 1997 to December 2004, 265 patients were diagnosed with DILD,and 140 (52.8%) of them were female. hepatocellular damage was the most common (72.1%), the incidence of death was 9.9% in patients with hepatocellular damage and 9.5% in patients with cholestatic/mixed damage (P < 0.05). There was no difference in age of dead and recovered patients. The proportion of females and males was similar in recovered and dead patients, no difference was observed in duration of treatment between the two groups. The serum total bilirubin (P < 0.001), direct bilirubin (P < 0.001) and aspartate transaminase (AST) (P = 0.013) values were higher in dead patients than in recovered patients. Chinese herbal medicine was the most frequently prescribed, accounting for 24.2% of the whole series. However, antitubercular drugs (3.4%) were found to be the primary etiological factor for fetal DILD. Factors associated with the development of fulminanthepatic failure were hepatic encephalopathy (OR = 43.66, 95% CI = 8.47-224.95, P < 0.0001), ascite (OR = 28.48, 95% CI = 9.26-87.58, P < 0.0001), jaundice (OR = 11.43, 95% CI = 1.52-85.96, P = 0.003), alcohol abuse (OR = 3.83, 95% CI = 1.26-11.67, P = 0.035) and direct bilirubin (OR = 1.93, 95% CI = 1.25-2.58, P = 0.012). CONCLUSION: Death occurs in 9.8% of patients with DILD. Chinese herbal medicine stands

关键词： Drug-induced liver disease Prognosis Prognostic marker Mortality

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Characterization of Cancer Stem Cells in Laryngeal Squamous Cell Carcinoma by Single-cell RNA Sequencing

引用

Genomics, Proteomics & Bioinformatics 2024年第4期22卷 119-132页

作者： Yanguo Li Chen Lin Yidian Chu Zhengyu Wei Qi Ding Shanshan Gu Hongxia Deng Qi Liao Zhisen Shen The Affiliated lihuili hospital Ningbo UniversityNingbo 315211China School of Public Health Health Science CenterNingbo UniversityNingbo 315211China Institute of Drug Discovery Technology Ningbo UniversityNingbo 315211China The ningbo Diagnostic Pathology Center Ningbo 315021China

Cancer stem cells(CSCs)constitute a pivotal element within the tumor microenvironment(TME),driving the initiation and progression of ***,the identification of CSCs and their underlying molecular mechanisms in laryngeal squamous cell carcinoma(LSCC)remains a formidable ***,we employed single-cell RNA sequencing of matched primary tumor tissues,paracancerous tissues,and local lymph nodes from three LSCC patients to comprehensively characterize the CSCs in *** distinct clusters of stem cells originating from epithelial populations were delineated and verified as CSCs and normal stem cells(NSCs),*** were abundant in the paracancerous tissues compared to those in the tumor *** showed high expression of stem cell marker genes such as PROM1,ALDH1A1,and SOX4,and increased the activity of tumor-related hypoxia,Wnt/β-catenin,and Notch signaling *** then explored the intricate crosstalk between CSCs and the TME cells and identified targets within the TME that related with *** also found eight marker genes of CSCs that were correlated significantly with the prognosis of LSCC ***,bioinformatics analyses showed that drugs such as erlotinib,OSI-027,and ibrutinib selectively targeted the CSC-specifically expressed *** conclusion,our results represent the first comprehensive characterization of CSC properties in LSCC at the single-cell level.

关键词： Laryngeal squamous cell carcinoma Cancer stem cell Single-cell RNA sequencing Cell-cell communication Therapeutic target.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Research status on immunotherapy trials of gastric cancer

引用

World Journal of Clinical Cases 2021年第21期9卷 5782-5793页

作者： Chao Liang Heng-Miao Wu Wei-Ming Yu Wei Chen Department of General Surgery Ningbo Medical Center Lihuili HospitalNingbo 315040Zhejiang ProvinceChina ningbo Medical Center lihuili hospital Ningbo UniversityNingbo 315040Zhejiang ProvinceChina Cancer Institute of Integrated Traditional Chinese and Western Medicine Zhejiang Academy of Traditional Chinese MedicineTongde Hospital of Zhejiang ProvinceHangzhou 310012Zhejiang ProvinceChina

The breakthrough of immune checkpoint inhibitor(ICI)therapy has created extensive opportunities for cancer ***,the block of programmed death-1/programmed death ligand 1(PD-L1)axis using ICIs has become a new therapeutic strategy to treat advanced gastric cancer(GC).However,in the past decade,single-arm and randomized trials for single-drug ICI therapy showed that the therapeutic effect was not satisfactory,including clinical trials for advanced ***,after selecting suitable predictive biomarkers and developing a combination of anti-angiogenic targeted drugs and other chemotherapeutic drugs,the objective response rate and progression-free survival of patients with gastric cancer were improved *** United States Food and Drug Administration has approved treatment with pembrolizumab for patients with advanced GC with PD-L1 expression or microsatellite instabilityhigh/mismatch repair *** this review,the updated data from the latest trial results of combination immunotherapy for GC are *** on the outcome of combination therapy,we discuss its possible molecular mechanism and summarize effective predictive *** also discuss possible problems stemming from results of other clinical trials of ICI treatment and propose other directions for ICI therapy.

关键词： Gastric cancer Immunotherapy Clinical trial Immune checkpoint inhibitor Neoadjuvant therapy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

miRNA-26b suppresses the TGF-β2-induced progression of HLE-B3 cells via the PI3K/Akt pathway

引用

International Journal of Ophthalmology(English edition) 2021年第9期14卷 1350-1358页

作者： En Shi Xiang-Nan Ye Liu-Yi Xie Department of Ophthalmology Ningbo Eye HospitalNingbo 315040Zhejiang ProvinceChina Department of Ophthalmology Ningbo Medical Center Lihuili HospitalNingbo 315041Zhejiang ProvinceChina Department of Ophthalmology Beilun District Peopled HospitalNingbo 315826Zhejiang ProvinceChina

AIM:To study the effect of mi R-26 b on lens epithelial cells induced by transforming growth factor beta(TGF-β)2 and the underlying signaling ***:Human lens epithelial cell line B-3(HLE-B3)was incubated with TGF-β2(5 ng/m L)and then transfected with mi R-26 b *** expression of mi R-26 b was determined using quantitative reverse transcriptase polymerase chain reaction(q RT-PCR),while 5’-bromodeoxyuridine(Brd U)and wound-healing assays were used to measure the growth and migration of HLE-B3 cells,*** expression of epithelialmesenchymal transition(EMT)markers and the activity of the phosphatidylinositol 3-kinase(PI3 K)/Akt pathway were measured by Western blotting assay and immunofluorescence *** microscopy was also used to observe cellular ***:The expression levels of mi R-26 b were significantly reduced in human posterior capsular opacification-attached lens tissue and TGF-β2-stimulated HLE-B3 *** the presence of TGF-β2,the growth,migration,and EMT of HLE-B3 cells were distinctly enhanced;these effects were attenuated by the administration of mi R-26 b ***,the overexpression of mi R-26 b significantly reduced upregulation of the PI3 K/Akt pathway when stimulated by TGF-β2 in HLE-B3 ***,the addition of an activator(740 Y-P)led to the upregulation of the PI3 K/Akt pathway and abolished the protective effect of mi R-26 b on the HLE-B3 cells that was mediated by TGF-β***:The mi R-26 b suppresses TGF-β2-induced growth,migration,and EMT in HLE-B3 cells by regulating the PI3 K/Akt signaling pathway.

关键词： posterior capsule opacification miRNA-26b proliferation migration epithelial-mesenchymal transition

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：