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Identifying the molecular basis of Jinhong tablets against chronic superficial gastritis via chemical profile identification and symptom-guided network pharmacology analysis

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Journal of Pharmaceutical Analysis 2022年第1期12卷 65-76页

作者： Danfeng Shi Lingxian Liu Haibo Li Dabo Pan Xiaojun Yao Wei Xiao Xinsheng Yao Yang Yu Institute of Traditional Chinese Medicine&Natural Products College of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drug ResearchJinan UniversityGuangzhou510632China Kanion Pharmaceutical Co. Ltd.State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical ProcessLianyungangJiangsu222001China State Key Laboratory of Quality Research in Chinese Medicine Macao Institute for Applied Research in Medicine and HealthMacao University of Science and TechnologyTaipaMacaoChina

Chronic superficial gastritis(CSG)is a common disease of the digestive system that possesses a serious *** tablet(JHT),a traditional Chinese medicine(TCM)prescription,exerts therapeutic effects against ***,the molecular basis of its therapeutic effect has not been ***,we employed ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry(UPLC-Q/TOF-MS)based chemical profile identification to determine the chemical components in ***,we applied network pharmacology to illustrate its molecular mechanisms.A total of 96 chemical constituents were identified in JHT,31 of which were confirmed using reference *** on the bioinformatics analysis using the symptom-guided pharmacological networks of“chi,”“blood,”“pain,”and“inflammation,”and target screening through the interaction probabilities between compounds and targets,matrix metalloproteinase 2(MMP2),dopamine d2 receptor(DRD2),and Aldo-keto reductase family 1 member B1(AKR1B1)were identified as key targets in the therapeutic effect exhibited by JHT against ***,according to the inhibitory activities presented in the literature and binding mode analysis,the structural types of alkaloids,flavonoids,organic acids,including chlorogenic acid(10),caffeic acid(13),(-)-corydalmine(33),(-)-isocorypalmine(36),isochlorogenic acid C(38),isochlorogenic acid A(41),quercetin-3-O-a-L-rhamnoside(42),isochlorogenic acid B(47),quercetin(63),and kaempferol(70)tended to show remarkable activities against *** to the above findings,we systematically identified the chemical components of JHT and revealed its molecular mechanisms based on the symptoms associated with CSG.

关键词： Chronic superficial gastritis Jinhong tablets UPLC-Q/TOF-MS Symptom-guided network pharmacology Molecular docking

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Work-family conflict and job burn-out among Chinese doctors:the mediating role of coping styles

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General Psychiatry 2018年第4期31卷 24-30页

作者： Ling Chen Junjun Liu Hailong Yang Hui Ma Hui Wang Yun Huang Hao Cheng Dianzhen Tang Miao Liu Houyuan Luo Haitao Qu Diwen Shen Ning Zhang Department of Clinical Psychology Jiangsu Province Geriatric InstituteNanjingChina Department of Psychiatry Nanjing Meishan HospitalNanjingChina Department of Clinical Psychology Nanjing Drum Tower Hospital Clinical College of Nanjing Medical UniversityNanjingChina Department of Clinical Psychology Nanjing Brain Hospital Affliated to Nanjing Medical UniversityNanjingChina Department of Neurology lianyungang Hospital of Traditional Chinese Medicine LianyungangChina Medical Insurance Audit Department Medical Insurance Management and Service BureauHangzhouChina Educational and Counseling Department of Mental Health Nanjing University of Posts and TelecommunicationsNanjingChina Customer Service Department Tuniu CorporationNanjingChina College of Education Weifang University of Science and TechnologyHuaifangChina Department of Educational Psychology University of AlbertaEdmontonAlbertaCanada Institute of Health Management Jiangsu Jiankang Vocational CollegeNanjingChina

Background Burn-out among doctors threatens their own health, and that of their patients. To identify risk factors of the doctor burn-out is vital to improving their health and increasing the quality of healthcare services. This study aims to explore the relationship between work-family confict （WFC） and burn-out among Chinese doctors and the mediating role of coping styles in this *** A cross-sectional survey was conducted in China, with a questionnaire packet which consisted of the Chinese Maslach Burnout Inventory （CMBI）, WFC Scale and the Simplifed Coping Style Questionnaire （SCSQ）. A total of 2530 doctors participated in the survey. Correlation analysis was performed to explore the relationship between CMBI, WFC and SCSQ scores. A linear regression model was set to determine the mediating role of coping styles on the relationship between WFC and *** Doctors who had higher scores on work interfering with family confict, reported more emotional exhaustion （r=0.514, P〈0.001） and had a sense of accomplishment （r= 0.149, P〈0.001）. Simultaneously, family interfering with work （FIW） was positively associated with all dimensions of burn-out （r=0.213, 0.504, 0.088, respectively, P〈0.001）. Coping styles had partial, complete and even mediating effects on the relationship between WFC and burn-out among Chinese *** WFC was correlated with burn-out, and coping style was a mediator in this relationship among Chinese doctors. Coping style was a positive resource against burn-out.

关键词： Work-family confict and job burn-out among Chinese doctors the mediating role of coping styles

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Cu-DNAzyme facilitates highly sensitive immunoassay

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Chinese Chemical Letters 2019年第9期30卷 1652-1654页

作者： Zhi Shan Mingsheng Lyu Dennis Curry David Oakley Ken Oakes Xu Zhang Verschuren Centre for Sustainability in Energy & the Environment Cape Breton University Sydney Now Scotia B1P 612 Canada College of Life Science Sichuan Agriculture University Yaan 625014 China Jiangsu Key Laboratory of Marine Bioresources and Environment Huaihai Institute of Technology Lianyungang 222005 China Department of Biology Cape Breton University Sydney Nova Scotia BJP 612 Canada Department of Chemistry Cape Breton University Sydney Nova Scotia B1P 612 Canada

Fast and sensitive antigen detection is important in biomedical research and development. Despite, being invented 48 years ago, the enzyme-linked immunosorbent assay(ELISA) remains one of the most successful and widely employed bioanalytical techniques in research and clinical diagnostics due to its reliability and simplistic design. Recently, nanotechnology has offered efficient signal reporting. In spite of some improvements in these systems, there are typically increased material costs involved and the need for expensive equipment or complicated chemical processes, thus negating any possible benefits over ELISA. Herein, we communicate a simple Cu-DNAzyme system for signal transduction of a CuO nanoparticle-labeled immunoassay. The reported immunoassay amplifies signal generation similar to traditional ELISA and is fast, simple, cost-effective, and sensitive, holding promise for biomedical applications and point-of-care testing.

关键词： Cu-DNAzyme Immunoassay ELISA CuO Nanoparticles

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Novel potential XOD inhibitory peptides derived from Trachinotus ovatus:Isolation,identification and structure-function analysis

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Food Bioscience 2022年第3期47卷 383-393页

作者： Mengfan Hou Huan Xiang Xiao Hu Shengjun Chen Yanyan Wu Jiachao Xu Xianqing Yang Key Laboratory of Aquatic Product Processing Ministry of Agriculture and RuralSouth China Sea Fisheries Research InstituteChinese Academy of Fishery SciencesGuangzhou510300China Co-Innovation Center of Jiangsu Marine Bio-industry Technology Jiangsu Ocean UniversityLianyungang222005China College of Food Science and Engineering Ocean University of ChinaQingdao266003China Collaborative Innovation Center of Provincial and Ministerial Co-construction for Marine Food Deep Processing Dalian116034China

This study aimed to isolate and identify the novel potential Xanthine oxidase(XOD)inhibitory peptides derived from Trachinotus ovatus hydrolysate(TOH)(hydrolysis by neutral protease),and investigate their structure-function ***,TOH was separated to four fractions(F1–F4)by gel filtration chromatography and F4 was chosen with higher XOD inhibitory rate(85.16%±0.566,25 mg/mL)and lower molecular weight(95.73%,<1000 Da).F4 was further characterized by reversed-phase high performance liquid chromatography(RP-HPLC)coupled with Nano-HPLC-MS/MS and four peptides(FPAW,LLPW,WLLP and FHLP)were identified with IC50 value of 3.81±0.18 mM,4.17±0.12 mM,43.06±0.73 mM,and over 50.00 mM *** can be concluded that Trp-containing peptides especially located in C-terminal could inhibit XOD ***,the XOD-inhibiting actions of these peptides involvedπ-πstacking(Phe914 and Phe1009 in XOD),hydrogen bonding via molecular ***-Burk equation showed the inhibition type of FPAW was a mixed *** dichroism(CD)spectra indicated FPAW could combine and change the secondary structure of ***,two peptides of FPAW and LLPW might have potential to be used to alleviate hyperuricemia in the future.

关键词： Trachinotus ovatus XOD inhibitory Activity Trp-containing peptides Nano-HPLC-MS/MS Molecular docking

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Evaluation of Reference Genes for Quantitative Real-Time PCR Analysis in Manila Clam Ruditapes philippinarum Under Hypoxic Stress

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Journal of Ocean University of China 2023年第4期22卷 1059-1067页

作者： JING Hao ZHOU Liqing GONG Miao TU Kang LIU Zhihong WU Biao SUN Xiujun College of Fisheries and Life Science Shanghai Ocean UniversityShanghai 201306China State Key Laboratory of Mariculture Biobreeding and Sustainable Goods Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Laboratory for Marine Fisheries Science and Food Production Processes Laoshan LaboratoryQingdao 266237China School of Marine Science and Fisheries Jiangsu Ocean UniversityLianyungang 222005China Putian Institute of Aquaculture Science of Fujian Province Putian 351100China

Quantitative real-time PCR(qRT-PCR)has been widely used for gene expression analysis,and selection of reference genes is a key point to obtain accurate *** find out optimal reference genes for qRT-PCR in Manila clam Ruditapes philippinarum in response to hypoxia,different tissues were used and compared to evaluate the stability of candidate reference genes under low oxygen stress(DO 0.5mgL^(−1) and DO 2.0mgL^(−1))and normal condition(DO 7.5mgL^(−1)).Seven candidate reference genes were selected to evaluate the stability of their expression *** reference genes were evaluated by Delta Ct,BestKeeper,NormFinder and geNorm,and then screened by RefFinder *** hypoxic stress of 0.5mgL^(−1),the most suitable reference gene for gill and hepatopancreas was RPL31,and the optimal reference genes for axe foot and adductor muscle were TUB and HIS,*** hypoxic stress of 2.0mgL^(−1),the most stable reference gene for gill and hepatopancreas was RPL31,and the optimal reference genes for axe foot and adductor muscle were RPS23 and EF1A,*** the normal condition,HIS and EF1A were identified as the optimal internal reference genes in gill and hepatopancreas respectively,and GFRP2 was the best internal reference gene for axe foot and adductor *** present findings will provide important basis for the selection of reference genes for qRT-PCR analysis of gene expression level in bivalves under hypoxic stress,which might be helpful for the analysis of other molluscs too.

关键词： clam reference gene hypoxia quantitative real-time PCR

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Study on Prediction Model of Number of Rainstorm Days in Summer Based on C5.0 Decision Tree Algorithm

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Meteorological and Environmental Research 2019年第2期10卷 56-60页

作者： Shi Yimin Chen Weiwei Zhu Yunfeng lianyungang Meteorological Bureau

Based on the data of daily precipitation in lianyungang area from 1951 to 2012 and various climate signal data from the National Climate Center website and the NOAA website,a model for predicting whether the number of rainstorm days in summer in lianyungang area is large was established by the classical C5. 0 decision tree algorithm. The data samples in 48 years( accounting for about 80% of total number of samples)was as the training set of a model,and the training accuracy rate of the model was 95. 83%. The data samples in the remaining 14 years( accounting for about 20% of total number of samples) were used as the test set of the model to test the model,and the test accuracy of the model was 85. 71%. The results showed that the prediction model of number of rainstorm days in summer constructed by C5. 0 algorithm had high accuracy and was easy to explain. Moreover,it is convenient for meteorological staff to use directly. At the same time,this study provides a new idea for short-term climate prediction of number of rainstorm days in summer.

关键词： C5. 0 algorithm Number of rainstorm days Prediction model

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连续排水边界下成层地基一维非线性固结解析解

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Journal of Central South University 2023年第2期30卷 503-516页

作者：宗梦繁吴文兵张驿梅国雄闻敏杰 School of Civil and Ocean Engineering Jiangsu Ocean UniversityLianyungang 222005China Faculty of Engineering China University of GeosciencesWuhan 430073China College of Civil Engineering and Architecture Guangxi UniversityNanning 530004China Research Center of Coastal Urban Geotechnical Engineering Zhejiang UniversityHangzhou 310058China

为了研究边界排水时间效应对固结过程的影响,引入连续排水边界研究成层地基一维非线性固结问题。通过引入孔隙比与有效应力之间的非线性关系,建立了瞬时荷载作用下成层地基固结问题的控制方程,进而获得按沉降定义和按超静孔隙水压力定... 详细信息

为了研究边界排水时间效应对固结过程的影响,引入连续排水边界研究成层地基一维非线性固结问题。通过引入孔隙比与有效应力之间的非线性关系,建立了瞬时荷载作用下成层地基固结问题的控制方程,进而获得按沉降定义和按超静孔隙水压力定义的平均固结度以及成层地基沉降的解析解。通过与有限差分解析解对比以及现有解析解对比验证了所得解析解的正确性。基于所得解,通过详细的参数分析研究了成层地基一维非线性固结特性。结果表明,排水界面参数对成层地基的固结性状有很大影响,固结速率随界面参数的增大而增大。在连续排水边界条件下,按沉降定义的平均固结度随有效应力比的增大而增大,而按超静孔隙水压力定义的平均固结度随有效应力比的增大而减小。

关键词：非线性固结成层地基连续排水边界界面参数解析解

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Cell-free plasma hypermethylated CASZ1, CDH13 and ING2 are promising biomarkers of esophageal cancer

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The Journal of Biomedical Research 2018年第6期32卷 424-433页

作者： Huanqiang Wang Congying Yang Siyuan Wang Tian Wang Jingling Han Kai Wei Fucun Liu Jida Xu Xianzhen Peng Jianming Wang Department of Public Health and Preventive Medicine Kangda College of Nanjing Medical University Department of Pathology The First People's Hospital of Lianyungang Department of Clinical Medicine Kangda College of Nanjing Medical University Department of Epidemiology Nanjing Medical University Department of Social Medicine and Health Education Nanjing Medical University The Innovation Center for Social Risk Governance in Health School of Public Health Nanjing Medical University

Identifying sensitive and specific biomarkers for early detection of cancer is immensely imperative for early diagnosis and treatment and better clinical outcome of cancer patients. This study aimed to construct a specific DNA methylation pattern of cancer suppressor genes and explore the feasibility of applying cell-free DNA based methylation as a biomarker for early diagnosis of esophageal squamous cell carcinoma(ESCC). We recruited early stage ESCC patients from Yangzhong County, China. The Illumina Infinium 450 K Methylation BeadChip was used to construct a genome-wide DNA methylation profile. Then, differentiated genes were selected for the validation study using the Sequenom MassARRAY platform. The frequency of methylation was compared between cancer tissues, matched cell-free DNAs and normal controls. The specific methylation profiles were constructed, and the sensitivity and specificity were calculated. Seven CG sites in three genes CASZ1, CDH13 and ING2 were significantly hypermethylated in ESCC as compared with normal controls. A significant correlation was found between the methylation of DNA extracted from cancer tissues and matched plasma cell-free DNA, either for individual CG site or for cumulative methylation analysis. The sensitivity and specificity reached 100% at an appropriate cut-point using these specific methylation biomarkers. This study revealed that aberrant DNA methylation is a promising biomarker for molecular diagnosis of esophageal cancer. Hypermethylation of CASZ1,CDH13 and ING2 detected in plasma cell-free DNA can be applied as a potential noninvasive biomarker for diagnosis of esophageal cancer.

关键词： esophageal neoplasms DNA methylation epigenesist biological markers tumor suppressor

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Electric field-driven building blocks for introducing multiple gradients to hydrogels

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Protein & Cell 2020年第4期11卷 267-285页

作者： Gang Xu Zhaozhao Ding Qiang Lu Xiaoyi Zhang Xiaozhong Zhou Liying Xiao Guozhong Lu David L K aplan Department of Orthopedics The Second Affiliated Hospital of Soochow UniversitySuzhou 215000China Department of Bums and Plastic Surgery Engineering Research Center of the Ministry of Education for Wound Repair TechnologyThe Affiliated Hospital of Jiangnan UniversityWuxi 214041China National Engineering Laboratory for Modern Silk&Collaborative Innovation Center of Suzhou Nano Science and Technology Soochow UniversitySuzhou 215123China Department of Orthopedics Affiliated Hospital of Xuzhou Medical UniversityLianyungang 222061China Department of Biomedical Engineering Tufts UniversityMedfordMA 02155USA

Gradient biomaterials are considered as preferable matrices for tissue engineering due to better simulation of native *** introduction of gradient cues usually needs special equipment and complex process but is only effective to limited *** of multiple gradients in the hydrogels remains ***,betasheet rich silk nanofibers(BSNF)were used as building blocks to introduce multiple gradients into different hydrogel systems through the joint action of crosslinking and electric *** blocks migrated to the anode along the electric field and gradually stagnated due to the solution-hydrogel transition of the systems,finally achieving gradient distribution of the blocks in the formed *** gradient distribution of the blocks could be tuned easily through changing different factors such as solution viscosity,which resulted in highly tunable gradient of mechanical *** blocks were also aligned under the electric field,endowing orientation gradient *** cargos could be loaded on the blocks and form gradient cues through the same crosslinking-electric field *** building blocks could be introduced to various hydrogels such as Gelatin and NIPAM,indicating the *** niches with multiple gradient cues could be achieved through the ***-based hydrogels with suitable mechanical gradients were fabricated to control the osteogenesis and ***-osteogenic gradient transition was obtained,which stimulated the ectopic osteochondral tissue regeneration in *** versatility and highly controllability of the strategy as well as multifunction of the building blocks reveal the applicability in complex tissue engineering and various interfacial tissues.

关键词： silk building blocks gradients hydrogel tissue regeneration

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A Preliminary Study of the Key Technologies in Rice Seed Production

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Asian Agricultural Research 2015年第12期7卷 90-92页

作者： Guodong HUA Guanxi LI Delong MENG Duoming WANG lianyungang Academy of Agricultural Sciences

To study the production technology of rice seeds,with Lianjing 7 as test material,this paper explores the effects of the key rice seed production,processing and storage technologies( such as nitrogen fertilizer application and management,weeding mode in seed breeding field,temperature control of mechanical drying and water control on seed storage) on the quality of rice seeds. The results show that the optimal application proportion of tillering stage dressing and earing fertilizer is 8: 2,followed by 7 : 3; the best time of weeding is booting stage,with the highest purity; the effect of drying and germination rate is best when the temperature is controlled at 44℃ in mechanical drying process; the seed storage quality and storage costs can be guaranteed when the water content of rice seed is controlled at 18%.

关键词： Rice seed Nitrogen fertilizer application and management Weeding period Mechanical drying Water content

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