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Journal of Integrative Agriculture 2019年第4期18卷 797-804页

作者： LU Yan-hui ZHENG Xu-song LU Zhong-xian State Key laboratory Breeding Base for Zhejiang Sustainable Pest and disease control Institute of Plant Protection and Microbiology Zhejiang Academy of Agricultural Sciences

Rice is an important staple food in China and it is at risk of attack by rice striped stem borer Chilo suppressalis, which occurs in most rice growing areas. In recent years, severe C. suppressalis outbreaks have been observed in China mainly due to changes in the rice cultivation systems, wide adoption of hybrid varieties and resistance to the dominant insecticides. Management relies primarily on chemical insecticides and resistance is an important contributing factor in these outbreaks. As a result, food safety of agricultural produce is reduced and the ecological and environmental integrities are threatened as well. Recently, environmentally friendly pest management measures, such as trap plants have been introduced for C. suppressalis management and this method can greatly reduce insecticide use. Our previous results indicated that the vetiver grass(Vetiveria zizanioides) is a dead-end trap plant that can effectively attract the adult females of C. suppressalis to lay eggs on it but where larvae are unable to complete their life cycle. This paper further explored the application of vetiver grass as a trap plant to manage C. suppressalis in the paddy fields. This environment-friendly tool can not only reduce C. suppressalis populations, it can also increase the diversity and abundance of natural enemies that can provide better environmental conditions for rice production.

关键词： rice vetiver trap plants Chilo suppressalis control application

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Rice science 2022年第3期29卷 268-276页

作者： WANG Chenjiaozi ZHAO Mei SHU Canwei ZHOU Erxun Guangdong Province Key laboratory of Microbial Signals and disease control Department of Plant PathologySouth China Agricultural UniversityGuangzhou 510642China

Trehalose metabolism is related to the sclerotial development of Rhizoctonia solani AG-1 IA,the causal agent of rice sheath blight(RSB).Here,we further elucidated the functions of three genes Rstre,Rstps1 and Rstpp that encode three key enzymes trehalase(TRE),alpha,alpha-trehalosephosphate synthase(TPS1)and trehalose 6-phosphate phosphatase(TPP)in the sclerotial development of *** AG-1 *** to the lack of a stable genetic transformation system for ***,the heterologous expression of these three genes in Pichia pastoris GS115 was *** results showed that reactive oxygen species(ROS)contents and enzyme activities in *** decreased significantly in the treatments of the fermentation broths of Rstps1 and Rstpp transformants,and that in the treatment of the fermentation broth of Rstre transformant visibly ***,the fermentation broths of the transformants of all the three genes were added to potato dextrose agar(PDA)medium for the cultivation of ***,as a result,the dry weight of sclerotia in each PDA plate containing the fermentation broths of Rstps1 and Rstpp transformants significantly increased compared with the control,and that of Rstre transformant obviously ***,178 proteins were found to interact with RSTPS1,and 16 of them were associated with *** together,the findings suggest that all these three genes related to trehalose metabolism play important roles in the sclerotial development of *** AG-1 IA,and can be used as new targets for the development of novel high-efficiency fungicides for the controlling of RSB.

关键词： Rhizoctonia solani AG-1 IA rice sheath blight reactive oxygen species trehalose metabolism gene functional analysis sclerotial development

Characterization of Xanthomonas citri *** from China based on spoligotyping

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Horticultural plant Journal 2022年第6期8卷 727-736页

作者： Wenting Li Zhenxi Li Jiaquan Huang Meirong Xu Zheng Zheng Xiaoling Deng Guangdong Province Key laboratory of Microbial Signals and disease control College of Plant ProtectionSouth China Agricultural UniversityGuangzhou 510642China

Xanthomonas citri ***(Xcc),a gram-negative bacterium,is the causal agent of citrus canker,one of the most devastating diseases threatening the citrus industry *** the diversity and population structure of Xcc is a prerequisite for disease epidemiological monitoring and effective disease *** characterization of the clustered regularly interspaced short palindromic repeats(CRISPR)/cas(CRISPR-associated proteins genes)system with a highly variable repeat number among species provides a new molecular typing method for bacterial genetic *** this study,we performed systematic in silico analyses of 28 Xcc genomes and identified a credible CRISPR/cas in Xcc *** analysis of CRISPR polymorphisms(repeat number and spacer types)in 129 Xcc A strains collected from six provinces in China identified 15 types of CRISPR arrays with 25 *** analysis of Xcc strains based on the CRISPR locus produced a more reliable and accurate typing result compared to the commonly used *** addition,seven associated cas genes—cas1,cas2,cas3,cas4,cas5,cas7(csd2),and cas8(csd1)—were found located adjacent to the CRISPR *** results showed>99%similarity of seven cas genes among Xcc *** analysis of spacer sequences showed that six spacers had possible phage/prophage *** characterization of the CRISPR/cas system among Xcc strains provided an updated strain typing method for Xcc diversity analysis and yielded a panoramic view of CRISPR evolution for further studies of Xcc-phage interactions.

关键词： Xanthomonas citri pv.citri CRISPR Diversity Spacers Typing method

Influence of Temperature on Symbiotic Bacterium Composition in Successive Generations of Egg Parasitoid, Anagrus nilaparvatae

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Rice science 2016年第4期23卷 203-210页

作者： WANG Xin XU Hong-xing LIU Shu-ping TANG Jiang-wu ZHENG Xu-song LV Zhong-xian State Key laboratory Breeding Base for Zhejiang Sustainable Pest and disease control Institute of Plant Protection and Microbiology Zhejiang Academy of Agricultural Sciences

Anagrus nilaparvatae is the dominant egg parasitoid of rice planthoppers and plays an important role in biological control. Symbiotic bacteria can significantly influence the development, survival, reproduction and population differentiation of their hosts. To study the influence of temperature on symbiotic bacterial composition in the successive generations of A. nilaparvatae, A. nilaparvatae were raised under different constant temperatures of 22 °C, 25 °C, 28 °C, 31 °C and 34 °C. Polymerase chain reaction-denaturing gradient gel electrophoresis was used to investigate the diversity of symbiotic bacteria. Our results revealed that the endophytic bacteria of A. nilaparvatae were Pantoea sp., Pseudomonas sp. and some uncultured bacteria. The bacterial community composition in A. nilaparvatae significantly varied among different temperatures and generations, which might be partially caused by temperature, feeding behavior and the physical changes of hosts. However, the analysis of wsp gene showed that the Wolbachia in A. nilaparvatae belonged to group A, sub-group Mors and sub-group Dro. Sub-group Mors was absolutely dominant, and this Wolbachia composition remained stable in different temperatures and generations, except for the 3rd generation under 34 °C during which sub-group Dro became the dominant Wolbachia. The above results suggest that the continuous high temperature of 34 °C can influence the Wolbachia community composition in A. nilaparvatae.

关键词： temperature Anagrus nilaparvatae Wolbachia bacterial community polymerase chain reaction-denaturing gradient gel electrophoresis rice brown planthopper

Fusarium-produced vitamin B6 promotes the evasion of soybean resistance by Phytophthora sojae

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Journal of Integrative plant Biology 2023年第9期65卷 2204-2217页

作者： Shuchen Wang Xiaoyi Zhang Zhichao Zhang Yun Chen Qing Tian Dandan Zeng Miao Xu Yan Wang Suomeng Dong Zhonghua Ma Yuanchao Wang Xiaobo Zheng Wenwu Ye Department of plant Pathology Nanjing Agricultural UniversityNanjing 210095China Key laboratory of plant immunity Nanjing Agricultural UniversityNanjing 210095China Key laboratory of Soybean disease and Pest control(Ministry of Agriculture and Rural Affairs) Nanjing Agricultural UniversityNanjing 210095China Key laboratory of Molecular Biology of Crop Pathogens and Insects Institute of BiotechnologyZhejiang UniversityHangzhou 310058China

plants can be infected by multiple pathogens concurrently in natural systems. However,pathogen–pathogen interactions have rarely been studied. In addition to the oomycete Phytophthora sojae, fungi such as Fusarium spp. also cause soybean root rot. In a 3-year field investigation, we discovered that P. sojae and Fusarium spp. frequently coexisted in diseased soybean roots. Out of 336 P. sojae–soybean–Fusarium combinations,more than 80% aggravated disease. Different Fusarium species all enhanced P. sojae infection when co-inoculated on soybean. Treatment with Fusarium secreted non-proteinaceous metabolites had an effect equal to the direct pathogen coinoculation. By screening a Fusarium graminearum mutant library, we identified Fusarium promoting factor of Phytophthora sojae infection 1(Fpp1),encoding a zinc alcohol dehydrogenase. Fpp1 is functionally conserved in Fusarium and contributes to metabolite-mediated infection promotion, in which vitamin B6(VB6) produced by Fusarium is key. Transcriptional and functional analyses revealed that Fpp1 regulates two VB6 metabolism genes, and VB6 suppresses expression of soybean disease resistance-related genes. These results reveal that co-infection with Fusarium promotes loss of P. sojae resistance in soybean, information that will inform the sustainable use of diseaseresistant crop varieties and provide new strategies to control soybean root rot.

关键词： fungal metabolites pathogen–host interaction Phytophthora sojae soybean root rot vitamin B6

Functional map of the macroglomerular complex of male Helicoverpaarmigera

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Insect Science 2023年第1期30卷 109-124页

作者： Xiao-Lan Liu Xi Chu Long-Long Sun Ya-Nan Wang Gui-Ying Xie Wen-Bo Chen Yang Liu Bente GBerg Shi-Heng An Gui-Rong Wang Xin-Ming Yin Xin-Cheng Zhao Henan International Joint laboratory of Green Pest control College of Plant ProtectionHenan Agricultural UniversityZhengzhouChina State Key laboratory for Biology of plant disease and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBejingChina Chemosensory laboratory Department of PsychologyNorwegian University of Science and TechnologyTrondheimNorway

The mechanism of sex pheromone reception in the male cotton bollworm Helicoverpa armigera has been extensively studied because it has become an important model system for understanding insect ***,the pathways of pheromone processing from the antenna to the primary olfactory center in *** have not yet been ***,the physiology and morphology of male *** olfactory sensory neurons(OSNs)were studied using single sensillum recording along with anterograde filling and intracellular recording with retrograde *** localized in type A sensilla responded to the major pheromone component cis-11-hexadecenal,and the axonal terminals projected to the cumulus(Cu)of the macroglomerular complex(MGC).The OSNs in type B sensilla respondcd to the bchavioral antagonist cis-9-tetradecenal,and the axonal terminals projected to the dorsomedial anterior(DMA)unit of the *** type C sensilla,there were 2 OSNs:one that responded to cis-9-tetradecenal and cis-11-hexadecenol with the axonal terminals projecting to the DMA,and another that responded to the secondary pheromone components cis-9-hexadecenal and cis-9-tetradecenal with the axonal terminals projecting to the dorsomedial posterior(DMP)unit of the *** A and type B sensilla also housed the secondary OSNs,which were silent neurons with axonal terminals projected to the glomerulus G49 and ***,the neural pathways that carry information on attractiveness and aversiveness in response to female pheromone components in *** exhibit distinct projections to the MGC units.

关键词： intracellular recording/retrograde filling macroglomerular complex olfactory sensory neuron single sensillum recording/anterograde filling

VSITA, an Improved Approach of Target Amplification in the Identification of Viral Pathogens

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Biomedical and Environmental Sciences 2018年第4期31卷 272-279页

作者： ZHANG Yi ZHANG Chen LI Bo LI Yang HE Xiao Zhou LI Acher WU Wei DUAN Su Xia QIU Fang Zhou WANG Ji SHEN Xin Xin YANG Meng Jie LI De Xin MA Xue Jun Key laboratory for Medical Virology National Health and Family Planning Commission National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention State Key laboratory of plant Genomics and National Center for plant Gene Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences Pediatric Research Institute Children's Hospital of Hebei Province

Objective Unbiased next generation sequencing（NGS） is susceptible to interference from host or environmental sequences. Consequently, background depletion and virome enrichment techniques are usually needed for clinical samples where viral load is much lower than background sequences. Methods A viral Sequence Independent Targeted Amplification（VSITA） approach using a set of non-ribosomal and virus-enriched octamers（V8） was developed and compared with traditionally used random hexamers（N6）. Forty-five archived clinical samples of different types were used in parallel to compare the V8 and N6 enrichment performance of viral sequences and removal performance of ribosomal sequences in the step of reverse transcription followed by quantitative PCR（qP CR）. Ten sera samples from patients with fever of unknown origin and 10 feces samples from patients with diarrhea of unknown origin were used in comparison of V8 and N6 enrichment performance following NGS analysis. Results A minimum 30 hexamers matching to viral reference sequences（sense and antisense） were selected from a dataset of random 4,096（4~6） hexamers（N6）. Two random nucleotides were added to the 5＇ end of the selected hexamers, and 480（30 × 4~2） octamers（V8） were obtained. In general, VSITA approach showed higher enrichment of virus-targeted c DNA and enhanced ability to remove unwanted ribosomal sequences in the majorities of 45 predefined clinical samples. Moreover, VSITA combined with NGS enabled to detect not only more viruses but also achieve more viral reads hit and higher viral genome coverage in 20 clinical samples with diarrhea or fever of unknown origin. Conclusion The VSITA approach designed in this study is demonstrated to possess higher sensitivity and broader genome coverage than traditionally used random hexamers in the NGS-based identification of viral pathogens directly from clinical samples.

关键词： Virus Next generation sequencing Non-ribosomal Virus targeted

Microencapsulated chlorpyrifos: Degradation in soil and influence on soil microbial community structures

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Journal of Environmental Sciences 2014年第11期26卷 2322-2330页

作者： Liezhong Chen Yanli Li Ting Wang Yali Jiang Kai Li Yunlong Yu College of Agriculture and Biotechnology Zhejiang University State Key laboratory Breeding Base for Zhejiang Sustainable Pest and disease control Institute of Plant Protection and MicrobiologyZhejiang Academy of Agricultural Sciences

Degradation kinetics of microencapsulated chlorpyrifos （CPF-MC） in soil and its influence on soil microbial community structures were investigated by comparing with emulsifiable concentration of chlorpyrifos （CPF-EC） in laboratory. The residual periods of CPF-MC with fortification levels of 5 and 20 mg/kg reached 120 days in soil, both of the degradation curves did not fit the first-order model, and out-capsule residues of chlorpyrifos in soil were maintained at 1.76 （±0.33） and 5.92 （±1.20） mg/kg in the period between 15 and 60 days, respectively. The degradation kinetics of CPF-EC fit the first-order model, and the residual periods of 5 and 20 mg/kg treatments were 60 days. Bacterial community structures in soil treated with two concentrations of CPF-MC showed similarity to those of the control during the test period, as seen in the band number and relative intensities of the individual band on DGGE gels （p 〉 0.05）. Fungal community structures were slightly affected in the 5 mg/kg treatments and returned to the control levels after 30 days, but initially differed significantly from control in the 20 mg/kg treatments （p 〈 0.05） and did not recover to control levels until 90 days later. The CPF-EC significantly altered microbial community structures （p 〈 0.05） and effects did not disappear until 240 days later. The results indicated that the microcapsule technology prolonged the residue periods of chlorpyrifos in soil whereas it decreased its side-effects on soil microbes as compared with the emulsifiable concentration formulation.

关键词： Pesticide controlled-release formulation Non-controlled-release formulation Side effects Soil microbiology

Tebufenozide resistance is associated with sex-linked inheritance in Plutella xylostella

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Insect Science 2015年第2期22卷 235-242页

作者： Guang-Chun Cao Zhao-Jun Han Entomology Department/Key laboratory of Monitoring and Management of plant diseases and Insects Ministry of Agriculture NanjingAgricultural University Nanjing State Key laboratory for Biology of plant disease and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural Sciences Beijing China

The diamondback moth （DBM）, Plutella xylostella （L.）, is a major pest of cruciferous crops. Tebufenozide, a novel nonsteroidal ecdysone agonist, exhibits good efficacy and has played an increasingly important role in the control of Lepidopteran pests in China. For its resistance management, the genetic basis of tebufenozide resistance was studied using a laboratory selected resistant strain of DBM （resistant ratio, RR = 268）. A series of crosses with laboratory susceptible and resistant strains revealed that tebufenozide resistance in this pest was partially biased toward female heredity, with a large difference in RR for F1 （RR = 29） and rF1 progeny （RR = 147）. The dominance calculated for these 2 cross progeny was -0.788 and 0.09, respectively. Further analysis showed that the susceptible male and female larvae were similar in theft sensitivity to tebufenozide, but the resistant female larvae showed significantly higher resistance than the resistant male larvae. The heredity of tebufenozide resistance in DBM might be linked with the W sex chromosome, which suggested that DBM has the ability to develop high levels of resistance to tebufenozide. This is the first report of sex-linked inheritance oftebufenozide resistance in P xylostella （L.）.

关键词： insecticide resistance Plutella xylostella sex linkage tebufenozide

Structural Analyses of Short-Chain Prenyltransfemses Identify an Evolutionarily Conserved GFPPS Clade in Brassicaceae plants

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Molecular plant 2016年第2期9卷 195-204页

作者： ChengyuanWang Qingwen Chen Dongjie Fan Jianxu Li Guodong Wang Peng Zhang National Key laboratory of plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China State Key laboratory of plant Genomics and National Center for plant Gene Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China University of Chinese Academy of Sciences Beijing 100039 China These authors contributed equally to this article Present address: National Institute for Communicable disease control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China

Terpenoids are the largest and most diverse class of plant-specialized metabolites, which function in diverse physiological processes during plant development. In the biosynthesis of plant terpenoids, short-chain prenyltransferases （SC-PTs）, together with terpene synthases （TPSs）, play critical roles in determining terpenoid diversity. SC-PTs biosynthesize prenyl pyrophosphates with different chain lengths, and these compounds are the direct precursors of terpenoids. Arabidopsis thaliana possesses a subgroup of SC-PTs whose functions are not clearly known. In this study, we focus on 10 geranylgeranyl pyro- phosphate synthase-like [GGPPSL] proteins, which are commonly thought to produce GGPP [C20]. We found that a subset of members of the Arabidopsis GGPPSL gene family have undergone neo- functionalization： GGPPSL6, 7, 9, and 10 mainly have geranylfarnesyl pyrophosphate synthase activity （C25; renamed AtGFPPS1, 2, 3, and 4）, and GGPPSL8 produces even longer chain prenyl pyrophosphate （〉C30; renamed polyprenyl pyrophosphate synthase 2, AtPPPS2）. By solving the crystal structures of AtGFPPS2, AtPPPS2, and AtGGPPS11, we reveal the product chain-length determination mechanism of SC-PTs and interpret it as a ＂three floors＂ model. Using this model, we identified a novel GFPPS clade distributed in Brassicaceae plants and found that the GFPPS gene typically occurs in tandem with a gene encoding a TPS, forming a GFPPS-TPS gene cluster.

关键词： terpenoid prenyltransferase molecular basis product length determination geranylfarnesyl diphos-phate synthase crystal structure