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Synthesis dynamic and developmental profile of prothoracicotropic hormone between diapause-and nondiapause-destined individuals in Helicoverpa armigera

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Chinese Science Bulletin 2007年第15期52卷 2095-2099页

作者： HOU ChengLin XU WeiHua Department of Molecular and cell biology School of Life Sciences University of Science and Technology of China Hefei 230027 China State Key laboratory of Biocontrol and School of Life Sciences Sun Yat-sen University Guangzhou 510275 China

Biosynthesis and secretion of prothoracicotropic hormone (PTTH) of diapause-and nondiapause-destined individuals in Helicoverpa armigera were studied using whole-mount immunocytochemistry and enzyme-linked immunosorbent assay (ELISA). The immunocytochemistry revealed that PTTH is expressed in two pairs of lateral neurosecretory cells of the brain. The presence of immunoreactivity has not significant difference between the brains of the diapause-and nondiapause-destined 6th instar larvae. However,the obvious differences of expressional pattern from day 4 pupae were observed be-tween the two types. PTTH titers in hemolymph from the 6th instar larvae to pharate adults were measured by the ELISA. Although there were similar titer changes between the two types of individuals at the larval stage,a significant difference from developmental expression was detected at the pupal stage,suggesting that the expression and secretion of PTTH does play a crucial role in regulation of pupal diapause of H. armigera.

关键词：激素免疫细胞化学酶免疫吸收剂生物合成

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Differentiation of human hepatoma cells induced by the expression of antisense PKCα

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Progress in Natural Science:Materials International 1998年第3期 80-86页

作者：王向阳柳惠图 Beijing 100875 Beijing Normal Universtiy China Department of biology The State Key laboratory of cell Proliferation and Regulation biology

The model of human hepatoma cells expressing antisense PKCα in vitro was previously establishedand characterized. It was found that PKCα acted as a positive regulator of proliferation in BEL-7402 human hepatomacells. Further studies indicated that cell morphology altered, dependent on serum recovered in human hepatoma cellsexpressing antisense PKCα (HT. AS6). In addition, RNA hybridization demonstrated the decreased expression ofoncogene Ha-ras, myc and increased expression of tumer-suppressor gene p53. In contrast to control cells (HTC1)and parent cells (BEL-7402), the ability of AFP secretion reduced and γ-GT activity decreased apparently. The re-sults indicate for the first time that the expression of antisense PKCα caused differentiation in human hepatoma cellsand PKCα may be an important chemotherapeutic target for the treatment of hepatomas.

关键词： protein kinase Cα,human hepatoma cells cell differentiation AFP γ-GT

Structural Analysis of Fibroin Heavy Chain Signal Peptide of Silkworm Bombyx mori

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Acta Biochimica et Biophysica Sinica 2006年第7期38卷 507-513页

作者： Sheng-Peng WANG~(1,2)Ting-Qing GUO~1 Xiu-Yang GUO~1 Jun-Ting HUANG~2 Chang-De LU~(1*) 1 Institute of Biochemistry and cell biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China 2 Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang 212018,China Institute of Biochemistry and cell biology Shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200031 China Sericultural Research Institute Chinese Academy of Agricultural SciencesZhenjiang 212018 China

To study the minimal length required for the secretion of recombinant proteins and silkproteins in posterior silk gland,the signal peptide(SP)of the fibroin heavy chain(FibH)of silkwormBombyx mori was systematically shortened from the *** effect on the secretion of protein wasobserved using enhanced green fluorescent protein(EGFP)as a *** of EGFP fusion proteinswas examined under fluorescence *** SPs with lengths of 20,18,16 and 12 *** directthe secretion of the reporter,yet those with lengths of 11, 10, 9, 8 and 1 a.a. can not. When the FibH SP wasshortened to 12a.a., the secretion efficiency was decreased slightly and cleavage occurred within *** *** the FibH SP were used,the secretion of fusion protein was normal and the cleavage sitewas between the Gly-Ser linker and Met,the starting amino acid of EGFP. These findings are applicable forthe expression of foreign proteins in silkworm silk *** cleavage site of the SP is discussed andcompared with the predictive results of the SignalP 3.0 online prediction program.

关键词： signal peptide fibroin heavy chain silkworm Bombyx mori rAcMNPV

Neuropathophysiology of functional gastrointestinal disorders

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World Journal of Gastroenterology 2007年第9期13卷 1313-1332页

作者： Jackie D Wood Department of Physiology and cell biology and Internal Medicine The Ohio State University College of Medicine Columbus Ohio 43210-1218 United States

The investigative evidence and emerging concepts in neurogastroenterology implicate dysfunctions at the levels of the enteric and central nervous systems as underlying causes of the prominent symptoms of many of the functional gastrointestinal disorders. Neurogastroenterological research aims for improved understanding of the physiology and pathophysiology of the digestive subsystems from which the arrays of functional symptoms emerge. The key subsystems for defecation-related symptoms and visceral hyper- sensitivity are the intestinal secretory glands, the musculature and the nervous system that controls and integrates their activity. Abdominal pain and discomfort arising from these systems adds the dimension of sensory neurophysiology. This review details current concepts for the underlying pathophysiology in terms of the physiology of intestinal secretion, motility, nervous control, sensing function, immuno-neural communication and the brain-gut axis.

关键词： Neurogastroenterology Visceral pain Diarrhea Irritable bowel syndrome Constipation Stress Enteritis Enteric nervous system Neuroimmune communication Mast cells

Cytocompatibility of regenerated silk fibroin film:a medical biomaterial applicable to wound healing

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第1期11卷 10-16页

作者： Tie-lian LIU Jing-cheng MIAO Wei-hua SHENG Yu-feng XIE Quan HUANG Yun-bo SHAN Ji-cheng YANG cell and Molecular biology Institute College of Medicine Soochow University Suzhou 215123 China laboratory Department of the Third Affiliated Hospital of Southern Medical University Guangzhou 510630 China Department of Renal Medicine Nantong Municipal No. 1 People's Hospital Nantong 226001 China

Objective： To explore the feasibility of using regenerated silk fibroin membrane to construct artificial skin substitutes for wound healing, it is necessary to evaluate its cytocompatibility. Methods： The effects of regenerated silk fibroin film on cytotoxicity, adhesion, cell cycle, and apoptosis of L929 cells, growth and vascular endothelial growth factor （VEGF） expression of ECV304 cells, and VEGF, angiopoietin-1 （Ang-1）, platelet-derived growth factor （PDGF） and fibroblast growth factor 2 （FGF2） expression of WI-38 cells were assessed by 3-（4,5）-dimethylthiahiazo （-z-yl）-3,5-di-phenytetrazoliumromide （MTT） assay, viable cell counting, flow cytometry （FCM）, and enzyme-linked immunosorbant assay （ELISA）. Results： We showed that the regenerated silk fibroin film was not cytotoxic to L929 cells and had no adverse influence on their adhesion, cell cycle or apoptosis; it had no adverse influence on the growth and VEGF secretion of ECV304 cells and no effect on the secretion of VEGF, Ang-1, PDGF and FGF2 by WI-38 cells. Conclusion： The regenerated silk fibroin film should be an excellent biomaterial with good cytocompatibility, providing a framework for reparation after trauma in clinical applications.

关键词： Regenerated silk fibroin film Cytocompatibility Cytotoxicity

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HTR2A agonists play a therapeutic role by restricting ILC2 activation in papain-induced lung inflammation

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cellular & Molecular Immunology 2023年第4期20卷 404-418页

作者： Zhishuo Wang Chenghua Yan Qizhen Du Yuying Huang Xuezhen Li Dan Zeng Ruizhi Mao Rama Krishna Gurram Shipeng Cheng Wangpeng Gu Lin Zhu Weiguo Fan Liyan Ma Zhiyang Ling Ju Qiu Dangsheng Li Enmei Liu Yaguang Zhang Yiru Fang Jinfang Zhu Bing Sun state Key laboratory of cell biology CAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesUniversity of Chinese Academy of Sciences320 Yueyang RoadShanghai 200031China College of Life Sciences Jiangxi University of Chinese MedicineNanchang 330004China Department of Respiratory Medicine Children's Hospital of Chongqing Medical University National Clinical Research Center for Child Health and DisordersMinistry of Education Key Laboratory of Child Development and DisordersChongqing Key Laboratory of PediatricsChongqingChina Department of Allergy Chongqing General HospitalChongqingChina Clinical Research Center and Division of Mood Disorders Shanghai Mental Health CenterShanghai Jiao Tong University School of MedicineShanghai 200030China laboratory of Immune System biology National Institute of Allergy and Infectious DiseasesNational Institutes of HealthBethesdaMD 20892USA CAS Key laboratory of Tissue Microenvironment and Tumor Shanghai Institute of Nutrition and HealthUniversity of Chinese Academy of SciencesChinese Academy of SciencesShanghai 200031China CAS Center for Excellence in Brain Science and Intelligence Technology Shanghai 200031China Shanghai Key laboratory of Psychotic Disorders Shanghai 201108China.

Group 2 innate lymphoid cells(ILC2s)are a category of heterogeneous cells that produce the cytokines IL-5 and IL-13,which mediate the type 2 immune ***,specific drug targets on lung ILC2s have rarely been *** studies have shown that type 2 cytokines,such as IL-5 and IL-13,are related to ***,we demonstrated the negative correlation between the depression-associated monoamine neurotransmitter serotonin and secretion of the cytokines IL-5 and IL-13 by ILC2s in individuals with ***,serotonin ameliorates papain-induced lung inflammation by suppressing ILC2 *** data showed that the serotonin receptor HTR2A was highly expressed on ILC2s from mouse lungs and human ***,an HTR2A selective agonist(DOI)impaired ILC2 activation and alleviated the type 2 immune response in vivo and in *** with ILC2-specific depletion of HTR2A(Il5^(cre/+)·Htr2a^(flox/flox)mice)abolished the DOI-mediated inhibition of ILC2s in a papain-induced mouse model of *** conclusion,serotonin and DOI could restrict the type 2 lung immune response,indicating a potential treatment strategy for type 2 lung inflammation by targeting HTR2A on ST2+ILC2s.

关键词： Serotonin(5-HT) HTR2A DOl Group 2 innate lymphoid cell Type 2 lung inflammation

Identification and Validation of SLC9A2 as A Potential Tumor Suppressor in Colorectal Cancer:Integrating Bioinformatics Analysis with Experimental Confirmation

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Current Medical Science 2024年第3期44卷 529-544页

作者： Yan-min LIU Tie-cheng YANG Xiao-chang FANG Li-jie YANG Li-wen SHI Hua-qiao WANG Ting-ting DOU Lin SHU Tian-Liang CHEN Jun HU Xiao-ming YU Xuan-fei LI Department of Gastroenterology the Central Hospital of WuhanKey Laboratory for Molecular Diagnosis of Hubei ProvinceTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430014China Department of Gastrointestinal Surgery Zhongnan Hospital of Wuhan UniversityWuhan430071China Clinical Medical Research Center of Peritoneal Cancer of Wuhan Wuhan430071China Hubei Provincial Clinical Research Center for Cancer Hubei Key Laboratory of Tumor Biological Behavi Wuhan430071China Department of General Surgery Taihe HospitalHubei University of MedicineShiyan442000China Department of Gastrointestinal Surgery Huangshi Central HospitalAffiliated Hospital of Hubei Polytechnic UniversityWuhan435001China

Objective To uncover the mechanisms underlying the development of colorectal cancer(CRC),we applied bioinformatic analyses to identify key genes and experimentally validated their possible roles in CRC onset and *** We performed Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis on differentially expressed genes(DEGs),constructed a protein-protein interaction(PPI)network to find the top 10 hub genes,and analyzed their expression in colon adenocarcinoma(COAD)and rectum adenocarcinoma(READ).We also studied the correlation between these genes and immune cell infiltration and prognosis and validated the expression of SLC9A2 in CRC tissues and cell lines using qRT-PCR and Western *** experiments were conducted in vitro to investigate the effects of SLC9A2 on tumor growth and *** We found 130 DEGs,with 45 up-regulated and 85 down-regulated in *** analysis indicated that these DEGs were primarily enriched in functions related to the regulation of cellular pH,zymogen granules,and transmembrane transporter *** pathway analysis revealed that the DEGs played pivotal roles in pancreatic secretion,rheumatoid arthritis,and the IL-17 signaling *** identified 10 hub genes:CXCL1,SLC26A3,CXCL2,MMP7,MMP1,SLC9A2,SLC4A4,CLCA1,CLCA4,and *** enrichment analysis showed that these hub genes were predominantly involved in the positive regulation of *** expression analysis revealed that CXCL1,CXCL2,MMP1,and MMP7 were highly expressed in CRC,whereas CLCA1,CLCA4,SLC4A4,SLC9A2,SLC26A3,and ZG16 were expressed at lower *** analysis revealed that 5 key genes were significantly associated with the prognosis of *** mRNA and protein expression levels of SLC9A2 were markedly reduced in CRC tissues and cell ***,SLC9A2 overexpression in SW480 cells led to a notable inhibition of cell proliferation,migration,and *** blotting analysis revealed tha

关键词： SLC9A2 colorectal cancer bioinformatic analysis biomarkers immune infiltrate

Rab1A mediates proinsulin to insulin conversion in β-cells by maintaining Golgi stability through interactions with golgin-84

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Protein & cell 2016年第9期7卷 692-696页

作者： Xiaojing Liu Zhenguo Wang Ying Yang Qingrun Li Rong Zeng Jiuhong Kang Jiarui Wu Key laboratory of Systems biology Institute of Biochemistry and Cell BiologyShanghai Institutes for Biological SciencesChinese Academy of Sciences Clinical and Translational Research Center of Shanghai First Maternity and Infant Health Hospital Shanghai Key Laboratory of Signaling and Disease ResearchSchool of Life Science and TechnologyTongji University School of Life Science and Technology ShanghaiTech University Shanghai Advanced Research Institute Chinese Academy of Sciences

Dear Editor,Insulin plays a critical role in mammalian glucose homeostasis,and dysfunctional insulin secretion results in *** maturation in pancreaticβcells can be generally divided into three stages:the first stage is the biosynthesis of proinsulin in the endoplasmic reticulum(ER),the second stage is the transport of proinsulin from the ER

关键词： Endoplasmic Reticulum Stress Proinsulin Insulin Content Golgi Cisterna Rab1A Expression

Effect of Brefeldin A on the Development of <i>Sargassum cymosum</i>Zygotes (Phaeophyceae, Fucales): Structural and Ultrasctructural Analysis

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American Journal of Plant Sciences 2020年第2期11卷 245-261页

作者： Ticiane Rover Zenilda L. Bouzon Carmen Simioni Plant cell biology laboratory Department of Cell Biology Embryology and Genetics Federal University of Santa Catarina Florianópolis SC Brazil Central laboratory of Electron Microscopy Federal University of Santa Catarina Florianópolis SC Brazil Postdoctoral Research of Post Graduate Program in cell biology and Development Department of Cell Biology Embryology and Genetics Federal University of Santa Catarina Florianópolis SC Brazil

To evaluate the effects of brefeldin A (BFA) on Golgi bodies and indirectly on the polarized development of S. cymosum zygotes, zygotes were cultured at concentrations of 4 μM, 8 μM, 16 μM and 32 μM of BFA, prepared in sterilized seawater. After 12 hours, the samples were fixed and processed for transmission electron microscopy (TEM), confocal laser scanning microscopy, light microscopy and cell counting. For recovery analysis, after treatment, the samples were again placed in sterile seawater for over 12 hours. In the control, after 12 hours, the embryos were adhered and with advanced stage of development, presenting up to 6 cell divisions. In the treated samples, the embryos did not adhere and only in the lower concentrations were they visualized with cellular divisions, is that in the highest concentration, the majority of the zygotes were dead. After recovery, embryos showed development only in the lowest concentrations. By confocal microscopy, the treated samples had an irregular distribution of chloroplasts and physodes. Also through TEM, it was possible to observe the loss of organization of Golgi bodies, in turn leading to the formation of vesicles and fusion of physodes. We can conclude that Golgi bodies are responsible for the production and secretion of molecules related to the adhesion and formation of cell wall membrane, also aiding in the polarization and orientation of physodes. The fused physodes occupied much of the cytoplasm, preventing other cytoplasmic processes, thus directly affecting the development of this alga.

关键词： Transmission Electron Microscopy Confocal Laser Scanning Microscopy Golgi Bodies Polarization Physodes