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Rational design of a biosensor circuit with semi-log dose-response function in Escherichia coli

Frontiers of Electrical and Electronic Engineering in China 2013年第3期8卷 209-220页

作者： Haoqian Zhang Ying Sheng Qianzhu Wu Ao Liu Yuheng Lu Zhenzhen Yin Yuansheng Cao Weiqian Zeng Qi Ouyang Peking University Team for the International Genetic Engineering Machine Competition (iGEM) Peking University Beijng 100871 China Peking-Tsinghua Joint Center for Life Sciences School of Life Sciences Peking University Beijing 100871 China Center for Quantitative Biology Peking University Beijng 100871 China State Key laboratory for Artificial Microstructures and Mesoscopic Physics School of Physics Peking University Beijing 100871. China

A central goal of synthetic biology is to apply successful principles that have been developed in electronic and chemical engineering to construct basic biological functional modules, and through rational design, to build synthetic biological systems with predetermined functions. Here, we apply the reverse engineering design principle of biological networks to synthesize a gene circuit that executes semi-log dose-response, a logarithmically linear sensing function, in Escherichia coil cells. We first mathematically define the object function semi-log dose-response, and then search for tri-node network topologies that can most robustly execute the object function. The simplest topology, transcriptional coherent feed-forward loop （TCFL）, among the searching results is mathematically analyzed; we find that, in TCFL topology, the semi-log dose-response function arises from the additive effect of logarithmical linearity intervals of Hill functions. TCFL is then genetically implemented in E. coil as a logarithmically linear sensing biosensor for heavy metal ions [mercury （II）]. functional characterization shows that this rationally designed biosensor circuit works as expected. Through this study we demonstrated the potential application of biological network reverse engineering to broaden the computational power of synthetic biology.

关键词： synthetic biology gene circuit design reverse engineering logarithmically linear sensing

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Efficient Removal of NO in The Air with Bi OI-calcined Photocatalyst under Solar Energy Irradiation

Efficient Removal of NO in The Air with Bi OI-calcined Photo...

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第十八届中国科协年会——分12大气雾霾及PM2.5...

作者： Wei WANG Junji CAO Yu HUANG Key Lab of Aerosol Chemistry & Physics Institute of Earth Environment Chinese Academy of Sciences State Key laboratory of Loess and Quaternary Geology(SKLLQG) Institute of Earth Environment Chinese Academy of Sciences

Nitrogen oxides(NO) and volatile organic compounds(VOCs), which are important precursor of secondary fine particle content, have crucial contributions to the formation of the ash haze phenomena1]. Hence, NOx abatement is a crucial issue that must be addressed. Recently, bismuth-layered compounds have received remarkable attention in photocatalytic degradation of organic and inorganic pollutants in view of their unique thin-layered structure, which benefits photo-induced charge separation and thus improves quantum yields2]. We report a flower-like BiO I photocatalyst with 41% NO removal efficiency under visible light through in situ thermal treatment of hierarchical architectures Bi OI synthesized using a facile hydrothermal process(Figure 1a-b). Experiment results and density functional theory calculation suggest that the signally visible light photocatalytic activity relative to the proper band energy, oxidizing the O molecule to ·OH radicals with two electrons on conduction band(Figure 1a and c). Multiple runs of photocatalytic experiments showed that BiO I was not significantly deactivated during long-term NO oxidation(Figure 1d). This result indicates that the BOC-based heterojunction is a stable photocatalyst with promising application in gaseous NOx abatement.

关键词： photocatalysis NO removal bismuth system

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Fast High-resolution Miniature Two-photon Microscopy for Brain Imaging in Freely-behaving Mice at the Single-spine Level

Fast High-resolution Miniature Two-photon Microscopy for Bra...

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中国神经科学学会第十二届全国学术会议

作者： Weijian Zong Runlong Wu Mingli Li Yanhui Hu Yijun Li Jinghang Li Hao Rong Haitao Wu Yangyang Xu Yang Lu Hongbo Jia Ming Fan Zhuan Zhou Yunfeng Zhang Aimin Wang Liangyi Chen Heping Cheng State Key laboratory of Membrane Biology Institute of Molecular Medicine PekingTsinghua Center for Life Sciences Peking University Department of Cognitive Sciences Institute of Basic Medical Sciences Department of Neurobiology Institute of Basic Medical Sciences Brain Research Instrument Innovation Center Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of Sciences State Key laboratory of Advanced Optical Communication System and Networks School of Electronics Engineering and Computer Science Peking University

Recent developments in miniaturized microscopes have furthered the quest to visualize brain activities and structural dynamics in animals engaged in self-determined behaviors. However, it remains an unmet challenge to resolve activity at single dendritic spines, the elemental units underlying neuronal computation, in freely-behaving animals. Here, we report the design, testing, and application of a fast, high-resolution, miniaturized two-photon microscope（FIRM-TPM） that accomplishes this goal. With a headpiece weighing 2.15 g and a new type of hollow-core photonic crystal fiber to deliver 920-nm femtosecond laser pulses, the FIRM-TPM is capable of imaging commonly used biosensors at high spatiotemporal resolution（0.64 μm laterally and 3.35 μm axially, 40 Hz at 256 × 256 pixels）. Its micro-electromechanical systems scanner also enables random-access capability and free-line scanning at up to 10,000 Hz. It compares favorably with benchtop two-photon microscopy and miniature wide-field fluorescence microscopy in the structural and functional imaging of Thy1-GFP-or GCa MP6 f-labeled neurons. Further, we demonstrate its unique application and robustness with hour-long recording of neuronal activities down to the level of spines in mice experiencing vigorous body and head movements or engaging in social interaction. Thus, our new generation miniature microscope provides neuroscientists the long-sought tool-of-choice for imaging the brain at the synaptic level in freely-behaving animals.

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Use of Open Source Software for the Development of Web GIS for Accessibility to Tourist Attractions

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Journal of Civil Engineering and Architecture 2013年第4期7卷 472-486页

作者： Maria Teresa Francoso Diogenes Cortijo Costa Mariana Mello Valin Rogerio Rodrigues Amarante Department of Geotechnics and Transports School of Civil Engineering Architecture and Urban Design University of CampinasSao Paulo 13083-852 Brazil JBS S.A. Corporation Lins 16402-161 Brazil Campinas Town Hall Americana 13468-100 Brazil

Based on the concepts of social inclusion, universal design and accessibility, this paper aims to describe the stages of the development and implementation of a Web GIS (geographic information system for the internet) based on OS (open source) software. The Web GIS developed is called "accessibility to Campinas tourist attractions", which provides information about cultural and natural attractions in Campinas/SP-Brazil, especially for people with disabilities or reduced mobility that prevents access these sites via public and private transportation. The verification of the existence or lack of accessibility equipment at bus stops and parking areas close to tourist attractions relies on current legislation (NBR 9050/1994) that establishes the right to accessibility for disabled people to transportation, local culture and leisure. An application that facilitates this recognition with low operating costs and high performance was developed using the OS software quantum GIS 1.0.2, Apache 2.2.4, Mapserver 5.0.2, I3GEO 4.0, PostgreSQL 8.3 and PostGIS 1.3.5. This software is able to integrate the management of tourist areas and the advertisement of tourist information regarding accessibility devices at sights recognized as tourist attractions by Campinas city hall.

关键词：旅游景点开源软件 GIS Web PostgreSQL 地理信息系统操作系统软件应用程序开发

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Isolation of Six Phosphate Dissolving Rhizosphere Bacteria （Bacillus subtilis） and Their Effects on the Growth, Phosphorus Nutrition and Yield of Maize （Zea mays L.） in Mali

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Journal of Agricultural Science and Technology(B) 2016年第2期6卷 93-107页

作者： Lamine Traore Hamadoun Babana Hani Antoun Messaoud Lahbib Ousmane Sacko Cindy Nakatsu Diane Stott Institute of Rural Economy BP 258 Bamako Mali University of Bamako BP 3206 Colline de Badalabougou Bamako Mali Department of Soils and Agri-Food Engineering & Horticulture Research Center Faculty of Agricultural and Food SciencesUniversity of Laval Quebec 2480 Canada Department of Agronomy Purdue University West Lafayette IN 47907-2053 USA National Soil Erosion Research laboratory Department of Agronomy Purdue University West Lafayette IN 47907-2053 USA

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Anti-aging theory of quantum nature and brain reprogramming

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Life Research 2019年第3期2卷 113-118页

作者： Yurii Pozniak 1Private laboratory of Advanced Studies Head Lab science of human life-stop aging. Rezond company KrakowPoland. Sevastopol CrimeaRussia

As many scientists believe, human aging is a very important part of the program of life-human activity rather than a disease. The human brain may be the controller, performer and participant of the natural program of life-human activity, from birth to death. The program of life-activities of the brain and the human body is very similar to a conventional computer program and it may also be malfunctioning. The work of the natural program of life-activity resembles the work of AI. Each program of life-activity of an individual is repeatedly subjected to natural correction of program tasks in the life of a biophysical being. However, in recent years, scientists from different countries began to seek to reprogram the brain and find the real cause of death. Some biologists and evolutionists believe that this process is nonrandom and that it is controlled by a kind of “death program.” So they call a special set of genes, forcing the body to become decrepit and die, giving way to a new generation of their own kind. The aim of our work is to design new technologies and methods to adjust the life-activity programs from biophysical views, increasing the useful life of bodies by delaying destructive processes and the aging process. Our life-activity program is carried out through the device and IT programs in a mobile application, using contact/contactless frequency code eff ects on the brain, which is decoded and accepted by the human brain for execution (reprogramming), as a natural addition and an integral part of the brain work. Code programs are compiled according to the working algorithms of the Cosmo-terrestrial structure of life-activity of biophysical objects on the planet Earth. Our technology is one of the varieties of brain-computer technology.

关键词： Brain reprogramming quantum medical Biophysics Armarium

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纳米制备与表征(英文)

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现代科学仪器 2003年第3期20卷 25-25页

作者：郭全民 Nanoscale Physics Research laboratory School of Physics and Astronomy University of Birmingham Birmingham B15 2TT UK

Are there great things to be discovered at the nanoscale?The answer is almost certainly'yes'.From the microscale to the nanoscale,it is far from just scaling down,it is a 'great leap forward'into a complete new regime where the physical and chemical properties of materials exhibit size dependent behaviour.A thorough understanding of the new physics and chemistry at the nanometer scale will lead to the design and fabrication of smart new functional materials and devices, for instance,electronic devices that operate on the basic principles of quantum *** will not go far without reliable measurement and characterisation ***,the boom in nanotechnology all started when the scanning tunnelling microscopy (STM) was invented in 1982, which enabled surface imagning with atomic resolution.

关键词：纳米材料制备表征扫描隧道显微镜 SPM

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