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Cultivation Techniques for Polygonatum Cyrtonema Hua in Hunan

Agricultural Science & Technology 2019年第2期20卷 28-33页

作者： SONG Rong YAN Bei YI Zi-li SUN Yi-zhong ZHU Xiao-qi ZHOU Jia-min CAO Liang LI Xiang-rong College of Bioscience and Biotechnology Hunan Agricultural UniversityChangsha 410128PRC Hunan Institute of Agricultural Environment and Ecology Changsha 410125PRC College of plant Protection Hunan Agricultural UniversityChangsha 410128PRC Xinhua Lvyuan Agriculture and Forestry Science and Technology Co. Ltd.Xinhua 417600PRC

This study works on setting a scientific standard for Polygonatum Cyrtonema Hua regarding its growth site condition,seed-stem choice,cultivation technique,field management,harvest,processing and *** aims at standardizing the cultivation of Polygonatum Cyrtonema Hua and providing technical support for the sustainable development of Polygonatum Cyrtonema Hua in Hunan province.

关键词： Traditional Chinese medicine Polygonatum cyrtonema Hua Technical regulation Hunan

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regulation of A 9-cis-epoxycarotenoid Dioxygenase(NCED)Gene from Raspberry on High Salinity and Cold Tolerance

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Journal of Northeast Agricultural University(English Edition) 2023年第2期30卷 32-42页

作者： Yang Guo-hui Li Bo-lin Wang Ze-min Yu Hang Wang Jia-ming Li Tie-mei The Key laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region) Ministry of Agriculture and Rural Affairs/National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions/College of Horticulture&Landscape ArchitectureNortheast Agricultural UniversityHarbin 150030China Basic Teaching Department Heilongjiang University of Industry and BusinessHarbin 150025China

Abscisic acid(ABA)is a plant hormone that plays an important role in plant development and abiotic *** 9-cis-epoxycarotenoid dioxygenase(NCED)is a key rate-limiting enzyme in the ABA biosynthetic *** physiological and molecular mechanisms of NCED regulating plant development and abiotic stress tolerance have been reported in many plant species,but gene function of RiNCEDs in Rubus idaeus *** rarely *** this study,the open reading frame(ORF)sequence of RiNCED2 in red raspberry fruit was isolated and the function of this gene under abiotic stress was *** RiNCED2 was induced by cold,high salinity,drought and ABA,it was highly expressed in new leaves as measured by real-time *** of RiNCED2 in Arabidopsis under both high-salt and cold stress increased ABA content,demonstrating that RiNCED2 was involved in ABA ***,the leaf wilting degree of transgenic Arabidopsis was less,while the content of malondialdehyde(MDA)was significantly reduced,and the chlorophyll content,proline content,peroxidase(POD),catalase(CAT)and superoxide dismutase(SOD)activities were significantly *** results indicated that overexpression of RiNCED2 enhanced the resistance of transgenic Arabidopsis to high salt and cold.

关键词： 9-cis-epoxycarotenoid dioxygenase raspberry high salt cold

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Identification of endophytic fungi responsive miRNA involved in the regulation of sesquiterpenoid biosynthesis in Atractylodes lancea

Identification of endophytic fungi responsive miRNA involved...

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中国植物学会第十六次全国会员代表大会暨85周年学术年会

作者： Jie Yuan Fei Chen Hurong Yang Piaoxue Chen Wei Zhang Mengjun Tang Xia Li Chuanchao Dai Jiangsu Key laboratory for Microbes and Functional Genomics Jiangsu Engineering and Technology Research Center for Industrialization of Microbial ResourcesCollege of Life SciencesNanjing Normal University Jiangsu High-Quality Rice Research and Development Center Nanjing Branch of China National Center for Rice ImprovementInstitute of Food CropsJiangsu Academy of Agricultural Sciences

Endophytes have been considered long-term safe and effective methods for inducing plant secondary metabolite ***,the underlying microRNAs associated with endophyte induced terpenoid biosynthesis are *** elucidate the molecular mechanism at the miRNA level,we identified endophytic fungiresponsive miRNAs in *** shoots and analyzed the function of their targets using a high-throughput platform.A total of 153 known miRNAs and 83 novel miRNAs were differentially expressed in *** after endophytes Gilmaniella ***12 ***,51,570 potential targets were *** ontology(GO) enrichment analyses showed prominent enrichment in signal transduction for the targets of down-regulated ***,novelir5,novelir44,and novelir37 were predicted to be involved in regulating sesquiterpenoid biosynthesis in ***.,Moreover,we proposed that novelir44 may be a hub miRNA functioning in regulating sesquiterpenoids biosynthesis by targeting 1-deoxyD-xylulose 5-phosphate synthase(DXS) and many transcription factors such as WRKY,APETALA2/Ethylene-Response Factors(AP2/ERF),basic Helix-Loop-Helix(bHLH),heat stress,and Nuclear transcription factor Y subunit A(NF-YA).This study provides the first insight into endophytes-induced plant terpenoid biosynthesis at the miRNA expression level and will provide knowledge about beneficial plant-endophyte interactions.

关键词： Endophytes Atractylodes lancea Sesquiterpenoids MicroRNA Transcription factors

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秸秆还田量对培肥农田黑土氮素初级转化速率的影响

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应用生态学报 2020年第12期31卷 4109-4116页

作者：霍海南李杰袁磊解宏图朱同彬 Christoph Müller 何红波张旭东中国科学院沈阳应用生态研究所沈阳110016 中国科学院大学北京100049 中国地质科学院岩溶地质研究所/自然资源部、广西壮族自治区岩溶动力学重点实验室广西桂林541004 Department of plant Ecology Justus-Liebig University GiessenHeinrich-Buff-Ring 2635392 GiessenGermany 辽宁省现代保护性耕作与生态农业重点实验室沈阳110016

秸秆还田是土壤培肥的重要措施之一,但培肥后土壤对氮素循环的调控功能是否具有可持续性以及与后续秸秆还田数量的关系尚不清楚。本研究对黑土旱地农田进行9年培肥处理后,设置了连续3年秸秆还田处理,秸秆还田量分别为年均秸秆产量(7500... 详细信息

秸秆还田是土壤培肥的重要措施之一,但培肥后土壤对氮素循环的调控功能是否具有可持续性以及与后续秸秆还田数量的关系尚不清楚。本研究对黑土旱地农田进行9年培肥处理后,设置了连续3年秸秆还田处理,秸秆还田量分别为年均秸秆产量(7500 kg·hm-2)的100%、67%、33%和0,探讨不同秸秆还田量对培肥土壤(0~10 cm)氮素转化过程的影响。结果表明:秸秆还田通过影响氮素初级转化速率,控制培肥土壤NH4+-N和NO3--N的产生与消耗过程。当秸秆还田量低于67%时,NH4+-N生成速率显著降低,而其消耗速率显著提高,土壤保留NH4+-N的能力下降,而NO3--N生成速率增加,土壤NO3--N固持能力下降,增加NO3--N的积累及淋溶损失风险。对于东北旱地农田,利用秸秆归还进行黑土培肥,需要不低于67%收获量的秸秆持续还田才能维持土壤对氮素的保持功能。

关键词：秸秆还田量 15N成对标记 MCMC数值优化模型氮素转化特征

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Effects of Arbuscular Mycorrhizal Fungi on the Physiology and Saponin Synthesis of Paris polyphylla var. yunnanensis at Different Nitrogen Levels

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Journal of Botanical Research 2023年第3期5卷 1-26页

作者： Can Huang Shubiao Qian Xiaoxian Li Xiahong He Shuhui Zi Congfang Xi Rui Shi Tao Liu College of Agriculture and Biotechnology Yunnan Agricultural UniversityKunmingYunnan650201China State Key laboratory of Conservation and Utilization of Bio-resources in Yunnan National&Local Joint Engineering Research Center on Gemplasm Innovation&Utilization of Chinese Medicinal Materials in Southwest ChinaYunnan Agricultural UniversityKunmingYunnan650500China Kunming Institute of Botany Chinese Academy of SciencesKunmingYunnan650201China Southwest Forestry University KunmingYunnan650224China Guangxi Subtropical Crops Research Institute NanningGuangxi530001China

Arbuscular mycorrhizal fungi(AMF)are important members of the plant microbiome and affect the uptake and transfer of mineral elements by forming a symbiotic relationship with plant ***(N),as an important mineral element,can directly affect plant growth and development at different N *** has been confirmed that inoculation with AMF can improve the efficiency of N utilization by ***,there are still fewer reports on the dynamic relationship between arbuscular mycorrhizal and plant secondary metabolites at different nitrogen *** this experiment,the physiological indexes and genes related to saponin synthesis were determined by applying different concentration gradients of nitrogen to the medicinal plant *** *** infested by AMF as the test *** was found that nitrogen addition increased the biomass,chlorophyll content,and nutrient content of above-and below-ground plant parts and increased the content of saponin content of *** *** to some extent,but AMF inoculation increased the saponin content of *** *** more *** inoculation also promoted the expression of genes related to the saponin synthesis pathway,including 3-hydroxy-3-methylglutaryl coenzyme A synthase(HMGS),squalene epoxidase 1(SE1),and cycloartenol synthase(CAS),which is in according with the accumulation of saponin in *** also may increase the saponin content of AMF plants by altering the expression of P450s and UGTs related to saponin synthesis.

关键词： Nitrogen Arbuscular mycorrhizal fungi Saponin P.polyphylla var.yunnanensis

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