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Phylogenetic relationships among Hystrix species and related species based on expressed sequence tag-polymerase chain reaction

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Journal of Systematics and Evolution 2011年第1期49卷 65-71页

作者： Hisashi TSUJIMOTO laboratory of plant genetics and Breeding Science Faculty of Agriculture Tottori University

Twelve species, including three Hystrix species, five Leymus species, Hordeum bogdanii, Pseudoroegneria spicata, Psathyrostachys huashanica, and Roegneria ciliaris, were used for expressed sequence tag-polymerase chain reaction (EST-PCR) assay. A total of 125 products were amplified by 72 sets of EST-PCR markers developed in barley, among which 106 (84.8%) products were found to be polymorphic. Each EST-PCR marker produced 0–6 polymorphic bands, with an average of 1.47. The relationship between H. duthiei ssp. duthiei and H. duthiei ssp. longearistata is close, but they are remote to H. patula. Hystrix duthiei ssp. duthiei and H. duthiei ssp. longearistata were clustered with Leymus species and Psathyrostachys huashanica, which suggested that they have close genetic relationships. The results of EST-PCR analysis are basically comparable with those obtained from studies on cytology, which indicated that EST-PCR can be used to assess the genetic relationships among the perennial species in Triticeae.

关键词： cluster analysis expressed sequence tag Hystrix molecular marker phylogenetic relationship.

Extensive profiling of the expressions of tRNAs and tRNA-derived fragments(tRFs)reveals the complexities of tRNA and tRF populations in plants

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Science China(Life Sciences) 2021年第4期64卷 495-511页

作者： Xuan Ma Chunyan Liu Xiaocong Kong Jing Liu Siju Zhang Shanshan Liang Weijiang Luan Xiaofeng Cao Tianjin Key laboratory of Animal and plant resistance College of Life SciencesTianjin Normal UniversinTianjin 300387China State Key laboratory of plant Genomics and National Center for plant Gene Research Institute of Genetics and Developmental BiologyChinese Academy of Sciences.Beijing 100101China

Evidence is emerging that t RNA-derived fragments(t RFs)are regulatory *** of t RFs in plants have been based on conventional small RNA sequencing,and focused on profiling of t RF-5 and t RF-3 species.A more comprehensive and quantitative analysis of the entire t RF population is highly ***,we employ t RNA-seq and YAMAT-seq,and develop a bioinformatics tool to comprehensively profile the expressions of t RNAs and t RFs in *** show that in Arabidopsis,approximately half of t RNA genes are extremely weakly expressed,accounting for only 1%of total t RNA abundance,while~12%of t RNA genes contribute to~80%of t RNA *** t RNA sequencings in various plants reveal that t RNA expression profiles exhibit a cross-species conserved *** characterizing the composition of a highly heterogeneous t RF population,we show that t RNA halves and previously unnoticed 10–16-nt tiny t RFs represent substantial *** highly accumulated 13-nt and 16-nt tiny t RFs in Arabidopsis indicate that tiny t RFs are not random t RNA degradation ***,we provide a user-friendly database for displaying the dynamic spatiotemporal expressions of t RNAs and t RFs in the model plants Arabidopsis and rice.

关键词： tRNA tRFs tRNA halves tiny tRFs tRNA-seq

Alteration of Terminal Heterochromatin and Chromosome Rearrangements in Derivatives of Wheat-Rye Hybrids

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Journal of genetics and Genomics 2013年第8期40卷 413-420页

作者： Shulan Fu Zhenling Lv Xiang Guo Xiangqi Zhang Fangpu Han State Key laboratory of plant Cell and Chromosome Engineering Institute of Geneticsand Developmental BiologyChinese Academy of Sciences Key laboratory of plant Breeding and genetics Sichuan Agriculture University

Wheat-rye addition and substitution lines and their self progenies revealed variations in telomeric heterochromatin and centromeres, Furthermore, a mitotically unstable dicentric chromosome and stable multicentric chromosomes were observed in the progeny of a Chinese Spring-lmperial rye 3R addition line. An unstable multicentric chromosome was found in the progeny of a 6R/6D substitution line. Drastic variation of terminal heterochromatin including movement and disappearance of terminal heterochromatin occurred in the progeny of wheat- rye addition line 3R, and the 5RS ditelosomic addition line. Highly stable minichromosomes were observed in the progeny ofa monosomic 4R addition line, a ditelosomic 5RS addition line and a 6R/6D substitution line. Minichromosomes, with and without the FISH signals for telomeric DNA （TTTAGGG）n, derived from a monosomic 4R addition line are stable and transmissible to the next generation. The results indicated that centromeres and terminal heterochromatin can be profoundly altered in wheat-rye hybrid derivatives.

关键词： Wheat-Rye addition lines Chromosome rearrangements Multiple centromeres Minichromosomes Heterochromatin

Therapeutic targeting of cellular prion protein: toward the development of dual mechanism anti-prion compounds

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Neural Regeneration Research 2025年第4期20卷 1009-1014页

作者： Antonio Masone Chiara Zucchelli Enrico Caruso Giovanna Musco Roberto Chiesa laboratory of Prion Neurobiology Department of NeuroscienceIstituto di Ricerche Farmacologiche Mario Negri IRCCSMilanItaly Biomolecular NMR Unit Division of Genetics and Cell BiologyIRCCS Ospedale San RaffaeleMilanItaly Department of Biotechnology and Life Sciences University of InsubriaVareseItaly

PrPSc,a misfolded,aggregation-prone isoform of the cellular prion protein(PrPC),is the infectious prion agent responsible for fatal neurodegenerative diseases of humans and other *** adopt different pathogenic conformations(prion strains),which can be resistant to potential drugs,or acquire drug resistance,posing challenges for the development of effective *** PrPCis the obligate precursor of any prion strain and serves as the mediator of prion neurotoxicity,it represents an attractive therapeutic target fo r prion *** this minireview,we briefly outline the approaches to target PrPCand discuss our recent identification of Zn(Ⅱ)-Bn PyP,a PrPC-targeting porphyrin with an unprecedented bimodal mechanism of *** argue that in-depth understanding of the molecular mechanism by which Zn(Ⅱ)-Bn PyP targets PrPCmay lead toward the development of a new class of dual mechanism anti-prion compounds.

关键词： anti-prion drug anti-PrPC antibody antisense oligonucleotide neurodegeneration pharmacological chaperone porphyrin prion disease PrPC degrader PrPC shedding zinc finger repressor

Characterization and fine mapping of the rice blast resistance gene Pia

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Science China(Life Sciences) 2011年第4期54卷 372-378页

作者： ZENG XiaoShan YANG XianFeng ZHAO ZhengHong LIN Fei WANG Ling PAN QingHua laboratory of plant resistance and genetics College of Natural Resources and Environment Sciences South China Agricultural University Guangzhou 510642 China Hunan Agricultural University Changsha 410128 China

Blast, caused by Magnaporthe oryzae, is one of the most widespread and destructive diseases of rice. Breeding durable resistant cultivars （cvs） can be achieved by pyramiding of various resistance （R） genes. Pia, carded by cv. Aichi Asahi, was evaluated against 612 isolates of M. oryzae collected from 10 Chinese provinces. The Pia gene expresses weak resistance in all the provinces except for Jiangsu. Genomic position-ready marker-based linkage analysis was carded out in a mapping population consisting of 800 F2 plants derived from a cross of Aichi Asahi×Kasalath. The locus was defined in an interval of approximately 90 kb, flanked by markers A16 and A21. Four candidate genes （Pia-1, Pia-2, Pia-3, and Pia-4）, all having the R gene conserved structure, were predicted in the interval using the cv. Nipponbare genomic sequence. Four candidate resistance gene （CRG） markers （A17, A25, A26, and A27）, derived from the four candidates, were subjected to genotyping with the recombinants detected at the flanking markers. The first three markers completely co-segregated with the Pia locus, and the fourth was absent in the Aichi Asahi genome and disordered with the Pia locus and its flanking markers, indicating that the fourth candidate gene, Pia-4, could be excluded. Co-segregation marker-based genotyping of the three sets of differentials with known R gene genotypes revealed that the genotype of A26 （Pia-3） perfectly matched the R gene genotype of Pia, indicating that Pia-3 is the strongest candidate gene for Pia.

关键词： rice blast resistance gene Pia t'me mapping genotype

Fine-mapping of an Arabidopsis cell death mutation locus

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Science China(Life Sciences) 2000年第2期43卷 138-145页

作者：牟中林戴亚李家洋 plant Biotechnology laboratory Institute of Genetics Chinese Academy of Sciences Beijing China

An Arabidopsis cell death mutation locus was mapped to chromosome 2 between lGS1 and mi421. The YAC clone ends, CIC9A3R, CIC11C7L, CIC2G5R and RFLP marker CDs3 within this interval, were used to probe TAMU BAC library and 31 BAC clones were obtained. A BAC contig encompassing the mutation locus, which consists of T6P5, T7M23, T12A21, T8L6 and T18A18, was identified by Southern hybridization with the BAC ends as probes. 11 CAPS and 12 STS markers were developed in this region. These results will facilitate map-based cloning of the genes and sequencing of the genomic DNA in this region.

关键词： Arabidopsis cell death mod1 mutant physical map CAPS marker.

Isolation and characterization of a new defense gene from soybean

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Science China(Life Sciences) 2001年第4期44卷 409-420页

作者：贺超英吴晓雷东方阳杜保兴张劲松陈受宜 plant Biotechnology laboratory Institute of Genetics Chinese Academy of Sciences Beijing China

A cDNA clone of a single-copy gene designated SbPRP was isolated and characterized from 2-week-old soybean seedlings. It putatively encodes a bimodular protein similar to develop-mentally regulated proteins in other plant species. The deduced amino acid sequence consists of 126 amino acids with a distinct proline-rich domain (17 amino acids) and a long hydrophobically cysteine-rich domain (84 amino acids), plus a signal peptide of 25 amino acids in N terminal. SbPRP mRNA transcripts accumulated in an organ specific manner. It can be detected in leaves and epicotyls of soybean seedlings, whereas virtually expression signal of SbPRP was not detected in cotyledons, hypocotyls and roots. Further Northern hybridization suggested that SbPRP steady-state mRNA level accumulated differentially not only in response to salicylic acid, but to the inoculation of soybean mosaic virus Sa strain. Also it was responsive to drought treatment and salt (NaCI) stress. Therefore it is likely that SbPRP functions as a defense gene in soybean.

关键词： soybean (Glycine max), defense gene, salicylic acid, water deficit, bimodular protein.

Expressions of Toll Like Receptor(TLR)Genes in Paralichthys olivaceus After Induced by Different Extracts of Edwardsiella tarda

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Journal of Ocean University of China 2022年第4期21卷 1027-1036页

作者： SHAN Yanan ZHENG Jinhui GAO Hong SUN Jinsheng Tianjin Key laboratory of Animal and plant resistance College of Life SciencesTianjin Normal UniversityTianjin 300387China

Toll like receptors(TLRs)are the main innate immune‘pattern recognition receptors’of animals,which play a central role in host cell recognition and responses to invasive pathogens,particularly common structures of microbial *** this study,the gene expression profiles of TLRs in the spleen,head kidney,gill,small intestine,liver,muscle,and heart of healthy Paralichthys olivaceus were detected by real-time quantitative PCR(qPCR).The TLR family members were widely expressed in different tissues with different basic expression *** highest expressions of TLR1,5m,7,8,9,14,and 21 were found in the spleen;the highest expressions of TLR3 and TLR21 were found in the gill;the highest expressions of TLR2 and 5s were found in the small *** second highest expressions of TLR3,7,and 8 were found in small *** gene expression profiles of TLRs stimulated with Edwardsiella tarda DNA,RNA,and lipopolysaccharide(LPS)were also detected in spleen,head kidney and ***9 and TLR21 were sensitive to *** DNA;TLR 8 and TLR21 were sensitive to *** RNA;and TLR1 and TLR14 were sensitive to *** *** expressions of the other TLR genes showed no significant *** results imply that the expressions of these TLR genes in *** are differently regulated in the whole body and play important roles in the immune response against *** infection.

关键词： Edwardsiella tarda Paralichthys olivaceus TLRs expression real-time fluorescence quantitative PCR

Leafy head formation of the progenies of transgenic plants of Chinese cabbage with exogenous auxin genes

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Cell Research 2000年第2期10卷 151-160页

作者： HE YU KE WAN XIN XUE YU DONG SUN XU HONG YU PING LIN LIU(Fax:021-64042090 E-mail: heyk@iris. ***)(National laboratory of plant Molecular genetics, ShanghaiInstitute of plant Physiology, Chinese Academy of Sciences,Shanghai 200032, China) National laboratory of plant Molecular Genetics Shanghai Institute of Plant Physiology Chinese Academy of Sciences Shanghai China

The experiment was performed to evaluate the progenies of plant lines transgenic for auxin synthesis genes derived from Ri T-DNA. Four lines of the transgenic plants were selfcrossed and the foreign auxin genes in plants of T5 generation were confirmed by Southern hybridization. Two lines, D1232 and D1653, showed earlier folding of expanding leaves than untransformed line and therefore had early initiation of leaf y head. Leaf cuttings derived from plant of transgenic line D1653 produced more adventitious roots than the control whereas the cuttings from folding leaves had much more roots than rosette leaves at folding stage, and the cuttings from head leaves had more roots than rosette leaves at heading stage. It is demonstrated that early folding of transgenic leaf may be caused by the relatively higher concentration of auxin. These plant lines with auxin transgenes can be used for the study of hormonal regulation in differentiation and development of plant organs nd for the breeding of new varietywith rapid growth trait.

关键词： Auxin genes Chinese cabbage leafy head transgenic plants