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Glucose alleviates cadmium toxicity by increasing cadmium fi xation in root cell wall and sequestration into vacuole in Arabidopsis

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Journal of Integrative plant Biology 2015年第10期57卷 830-837页

作者： Yuan-Zhi Shi Xiao-Fang Zhu Jiang-Xue Wan Gui-Xin Li Shao-Jian Zheng Tea Research Institute of the Chinese Academy of Agricultural Sciences and the Key laboratory for plant Biology and Resource Application of Tea the Ministry of Agriculture State Key laboratory of plant physiology and Biochemistry College of Life SciencesZhejiang University State Key laboratory of soil and Sustainable Agriculture China Institute of Soil Science the Chinese Academy of Science College of Agronomy and Biotechnology Zhejiang University

Glucose (Glu) is involved in not only plant physiological and developmental events but also plant responses to abiotic stresses. Here, we found that the exogenous Glu improved root and shoot growth, reduced shoot cadmium (Cd) concentration, and rescued Cd-induced chlorosis in Arabidopsis thaliana (Columbia ecotype, Col-0) under Cd stressed conditions. Glucose increased Cd retained in the roots, thus reducing its translocation from root to shoot significantly. The most Cd retained in the roots was found in the hemicellulose 1. Glucose combined with Cd (Glu t Cd) treatment did not affect the content of pectin and its binding capacity of Cd while it increased the content of hemicelluloses 1 and the amount of Cd retained in it significantly. Furthermore, Leadmium Green staining indicated that more Cd was compartmented into vacuoles in Glu t Cd treatment compared with Cd treatment alone, which was in accordance with the significant upregulation of the expression of tonoplast-localized metal transporter genes, suggesting that com-partmentation of Cd into vacuoles also contributes to the Glu-alleviated Cd toxicity. Taken together, we demonstrated that Glu-alleviated Cd toxicity is mediated through increas-ing Cd fixation in the root cell wall and sequestration into the vacuoles.

关键词： Arabidopsis cadmium tolerance glucose plant cell wall plant vacuole

Jasmonic acid alleviates cadmium toxicity in Arabidopsis via suppression of cadmium uptake and translocation

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Journal of Integrative plant Biology 2020年第2期62卷 218-227页

作者： Gui Jie Lei Li Sun Ying Sun Xiao Fang Zhu Gui Xin Li Shao Jian Zheng State Key laboratory of plant physiology and Biochemistry College of Life SciencesZhejiang UniversityHangzhou 310058China State Key laboratory of soil and Sustainable Agriculture Institute of Soil ScienceChinese Academy of SciencesNanjing 210008China College of Agronomy and Biotechnology Zhejiang UniversityHangzhou 310058China

Jasmonic acid(JA)is thought to be involved in plant responses to cadmium(Cd)stress,but the underlying molecular mechanisms are poorly ***,we show that Cd treatment rapidly induces the expression of genes promoting endogenous JA synthesis,and subsequently increases the JA concentration in Arabidopsis ***,exogenous methyl jasmonate(MeJA)alleviates Cd-generated chlorosis of new leaves by decreasing the Cd concentration in root cell sap and shoot,and decreasing the expression of the AtIRT1,AtHMA2 and AtHMA4 genes promoting Cd uptake and long-distance translocation,*** contrast,mutation of a key JA synthesis gene,At AOS,greatly enhances the expression of AtIRT1,AtHMA2 and AtHMA4,increases Cd concentration in both roots and shoots,and confers increased sensitivity to *** Me JA recovers the enhanced Cd-sensitivity of the ataos mutant,but not of atcoi1,a JA receptor *** addition,exogenous Me JA reduces NO levels in Cd-stressed Arabidopsis root *** together,our results suggest that Cd-induced JA acts via the JA signaling pathway and its effects on NO levels to positively restrict Cd accumulation and alleviates Cd toxicity in Arabidopsis via suppression of the expression of genes promoting Cd uptake and long-distance translocation.

关键词： Jasmonic acid cadmium uptake translocation

Responses of Aerobic Rice (Oryza sativa L.) to Iron Deficiency

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Journal of Integrative Agriculture 2012年第6期11卷 938-945页

作者： SHI Rong-li HAO Hong-mei FAN Xiao-yun Md Rezaul Karim ZHANG Fu-SUO ZOU Chun-qin Key laboratory of plant-soil Interaction Ministry of Education/Center for ResourcesEnvironment and Food SecurityDepartment of Plant NutritionChina Agricultural University

Aerobic rice has the advantage of saving water. Most published work has focused on improving its yield, while few reported on its micronutrient status. In fact, Fe deficiency is a common nutritional problem in the production of aerobic rice. Short- term hydroponic culture experiments were conducted to study the response of aerobic rice to Fe deficiency and the effect of root exudates from Fe-deficient wheat on its Fe uptake ability. The results indicate that the amount of phytosiderophores （PS） released from aerobic rice did not increase under Fe deficient conditions. The Fe（III） reducing capacity of Fe-deficient aerobic rice did not increase and the solution pH did not decrease significantly. What＇s more, no obvious swelling was observed in the root tips. Aerobic rice did not show special responses to improve their Fe nutrition under Fe deficiency as both strategy I and II plants though they were very sensitive to Fe deficiency. This may be a reason which causes Fe deficiency problem in aerobic rice. However, root exudates from Fe-deficient wheat （PSw） could improve its Fe nutrition in the presence of insoluble Fe（OH）3. This suggests that aerobic rice could utilize Fe activated by PSw.

关键词： aerobic rice Fe(III)-reduction iron nutrition phytosiderophore release root tip swelling

Isolation and Characterization of Chlorothalonil-Degrading Bacterial Strain H4 and Its Potential for Remediation of Contaminated soil

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Pedosphere 2014年第6期24卷 799-807页

作者： ZHANG Man-Yun TENG Ying ZHU Ye WANG Jun LUO Yong-Ming P.CHRISTIE LI Zhen-Gao T.K.UDEIGWE Key laboratory of soil Environment and Pollution Remediation Institute of Soil ScienceChinese Academy of Sciences University of Chinese Academy of Sciences Department of plant and soil Science Texas Tech.University

A chlorothalonil(CTN)-degrading bacterial strain H4 was isolated in this study from a contaminated soil by continuous enrichment culture to identify its characteristics and to investigate its potential for remediation of CTN in contaminated soil. Based on the morphological, physiological and biochemical tests and 16 S r DNA sequence analysis, the strain was identified as Stenotrophomonas sp. After liquid culture for 7 d, 82.2% of CTN was removed by strain H4. The isolate could degrade CTN over a broad range of temperatures and p H values, and the optimum conditions for H4 degradation were p H 7.0 and 30℃. Reintroduction of the bacteria into artificially contaminated soil resulted in substantial removal of CTN(> 50%) after incubation for 14 d. soil samples treated by H4 showed significant increases(P soil dehydrogenase activity, soil polyphenol oxidase activity, average well-color development obtained by the Biolog Eco plate TM assay and Shannon-Weaver index, compared with the control. Strain H4 might be a promising candidate for application in the bioremediation of CTN-contaminated soils.

关键词： biodegradation fungicide soil bioremediation Stenotrophomonas sp.

Refinement of Methodology for Cadmium Determination in soil Micro-Arthropod Tissues

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Pedosphere 2017年第3期27卷 491-501页

作者： ZHU Dong KE Xin WU Longhua HUANG Yujuan Peter CHRISTIE LUO Yongming Key laboratory of soil Environment and Pollution Remediation Institute of Soil Science Chinese Academy of Sciences University of Chinese Academy of Sciences Institute of plant physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Key laboratory of Coastal Zone Environmental Processes Yantai Institute of Coastal Zone Research Chinese Academy of Sciences

Highly precise and reliable determination of heavy metals in soil micro-arthropod tissues remains a challenge because of the small size of the animals and their typical low abundance in metal-contaminated agricultural soils. The present study sought to develop a method for cadmium （Cd） determination in soil micro-arthropods by optimizing the sample digestion procedure, reducing sample weight, modifying sample pre-treatment and validating the methodology with field samples. The optimized digestion conditions comprised a sample mass of 50-150 μg, digestion reagent of nitric acid：hydrogen peroxide （3：1）, digestion temperature of 105 ℃, digestion period of 3 h and digestion volume of 30 μL. Defecation of the standard Collembola Folsomia candida （92 h） and the indigenous Collembola Onychiurus yodai （42 h） and ultrasonic cleaning of F. candida increased the accuracy of Cd determination. The recovery of Cd using the refined procedure was 98.9% and the limits of detection and quantification were 0.002 and 0.008 μg L-1, respectively. The within-batch precision values were 〈 3%. The Cd concentrations in the tissues of the Collembola Isotorna sp. collected from a range of metal-contaminated fields determined by the improved method were consistent with the Cd concentrations in the field soils. The results indicate that the optimized method can be used for more accurate or reliable determination of Cd concentrations in soil micro-arthropod tissues.

关键词： digestion defecation Folsomia candida micro-samples predatory mite sample pre-treatment ultrasonic cleaning

Toxicity of lead pollution to the collembolan Folsomia candida in Ferri-Udic Cambosols

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Pedosphere 2021年第4期31卷 627-637页

作者： Ying DING Zhu LI Xin KE Longhua WU Shengpeng ZUO Key laboratory of soil Environment and Pollution Remediation Institute of Soil ScienceChinese Academy of SciencesNanjing 210008(China) College of Environmental Science and Engineering Anhui Provincial Engineering Laboratory of Water and Soil Pollution Control and RemediationAnhui Normal UniversityWuhu 241003(China) Institute of plant physiology and Ecology Shanghai Institute for Biological SciencesChinese Academy of SciencesShanghai 200032(China)

Toxic effect of lead(Pb) pollution on Collembola in soils has seldom been studied in depth, which is especially true for growth responses since the juveniles are very small and numerous. A single species test was conducted using the collembolan Folsomia candida as the indicator species, and soil Pb pollution(at Pb concentrations of 0, 300, 600, 1 200, 2 400, and 4 800 mg kg^(-1)) was simulated by mixing Pb(NO_(3))_(2) with a natural unpolluted soil. Adult survival, reproduction, body length, and body Pb concentration were determined. Adult survival and reproduction started to decrease at soil Pb concentrations of 1 200 and 600 mg kg^(-1), respectively. Lead concentrations in adult and juvenile bodies increased with increasing Pb concentration in soil. The percentage and individual abundances based on body length analysis showed that the larger adults(2.1–2.6 mm, P soil Pb concentration than the intermediate(1.9–2.1 mm, P soil Pb concentration than the intermediate(0.6–1.0 mm, P soil Pb concentration, suggesting that larger individuals were more sensitive to soil Pb pollution. Therefore, body length features, especially the percentage and abundance of larger individuals, would be potential bio-indicators of soil Pb pollution and possibly other types of soil pollution.

关键词： bio-indicators body length Collembola large individuals soil Pb pollution

Effects of specific expression of iaaL gene in tobacco tapetum on pollen embryogenesis

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Science China(Life Sciences) 1997年第4期40卷 384-391页

作者：杨洪全卫志明许智宏 State Key laboratory of plant Molecular Genetics Shanghai Institute of Plant Physiology Chinese Academy of Sciences Shanghai 200032 China

The indoleacetic-acid-lysine synthetase (iaaL) gene from Pseudomonas syringae subsp. savastanoi was fused to tobacco tapetum-specific expression promoter TA29, and introduced into tobacco. The expression pattern of this chimeric gene was studied, and the endogenous indoleacetic acid (IAA) levels in different organs were assayed. The results demonstrated that TA29 promoter was only able to direct the specific expression of iaaL gene in transgenic tobacco anther, and resulted in the decrease of endogenous IAA levels in transgenic tobacco anther. No significant phe-notype variation was observed among the transgenic plants at the whole plant level. However, the percentage of pollen embryogenesis was reduced to 11 % when anthers of the transgenic plants were cultured on the modified hormone-free Nistch H (NH) medium, while those of both CK1 and CK2 (see sec. 1.2.2) were more than 50% ; when the an-thers were cultured on NH medium supplemented with 0. 2 mg/L IAA, the percentage of pollen embryogenesis re-stored to the same level of that of the wild type (up to 55. 7% ). This study demonstrates that the IAA metabolism in anther tapetum cells is of significant importance to the androgenic development in anther culture.

关键词： tapetum-specific expression indoleacetic-acid-lysine synthetase gene transgenic tobacco pollen embryogenesis.

Mutant Selection of Glyphosate-tolerant Soybean

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Chinese Science Bulletin 1993年第21期38卷 1816-1820页

作者：何卓培徐淑平 Jun 徐俊 State Key laboratory of plant Molecular Genetics Shanghai Institute of Plant PhysiologyAcademia SinicaShanghai 200032PRC

Over 10% loss of agricultural incomes results from the weeds in the crop fields. But the cost of developing a new herbicide is 20-100 times as much as that of breeding a new cultivar of herbicide-tolerant crop. So such crops are bred abroad and there appear an Atrazine-tolerant cultivar of rape by hybridization breeding, an Imazapyr-tolerant line of maize selected from tissue culture, a Chlorsulfuron-tolerant soybean

关键词： herbicide tolerance glyphosate soybean mutant selection

Effects of copper, lead and zinc in soil on egg development and hatching of Folsomia candida

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Insect Science 2009年第1期16卷 51-55页

作者： Jie Xu Yin Wang Yong-Ming Luo Jing Song Xin Ke Institute of plant physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghal soil and Environment Bioremediation Research Centre Institute of Soil Sciences Chinese Academy of Sciences Nanjing China

Effects of CaCl2, CuCl2, ZnCl2 and PbCl2 on development and hatching success of eggs of Folsomia candida （Collembola） were studied under laboratory conditions. Thousands of healthy eggs from synchronized cultures were incubated in soils treated with different concentrations of the metals. Compared with the water control, egg hatch significantly decreased when concentrations of Cu, Pb and Zn reached 400, 1 600 and 800 mg/kg dry soil, respectively. Values of EC50（hatching）, calculated according to the exponential model （with 95% confidence limits in brackets）, were 625 （407-875）, 2 361 （2 064-2 687） and 1 763 （1 548-2 000） mg/kg dry soils for Cu, Pb and Zn, respectively. When Cu concentration reached 1 600 mg/kg dry soil, eggs became green and the percentage of green eggs changed from 5%-20% after incubation for 2 days to 15%- 30% after incubation for 4 days. At 3 200 mg Cu/kg dry soil, tissues inside eggs were black and shrunken.

关键词： metal pollution soil springtail hatching success metamorphosis toxicity test,ultrathin sectioning