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Acta Oceanologica Sinica 2020年第10期39卷 162-170页

作者： Bingxin Huang Linhong Teng Jingjing Jiang Lanping Ding Collage of Life Sciences Tianjin Normal UniversityTianjin 300387China Institute of Oceanology Chinese Academy of SciencesQingdao 266071China Tianjin Key laboratory of Animal and plant Resistance Tianjin 300387China

Due to extensive morphological plasticity,the taxonomy of Cladophora species remains *** one of the widely distributed species,*** was reported to hold many varieties,which make it difficult to identify the *** study explored the morphology diversity of nine *** samples collected along the coast of western Yellow *** samples showed extremely varied characteristics,by which one cannot classify them ***,18S rDNA and nuclear ribosomal DNA internal transcribed spacer regions(ITS)sequences were employed to delimit *** 18S rDNA,sequence similarity ranged from 99.6%to 100%.For ITS region,the similarity ranged from 98.7%to 100%.Molecular data strongly suggested that the morphologically heterogeneous samples were actually the same *** comparison of the samples revealed that the taxonomy criteria including branching pattern and density,thallus color,height and texture varied widely,influenced by environmental conditions and age of ***,cell dimensions,as the relatively stable criterion,also exhibited intraspecific *** application of 18S rDNA and ITS sequences indicated that molecular method can be a powerful assistant as DNA barcodes to traditional morphology taxonomy.

关键词： Cladophora gracilis morphology observation molecular identification 18S rDNA ITS

Characterization of laccase gene StLAC6 and its involvement in the pathogenicity and peroxisome function in Setosphaeria turcica

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Journal of Integrative Agriculture 2022年第7期21卷 2019-2030页

作者： LIU Ning ZHANG Qian-qian JIA Hui ZHAO Bin ZHU Zi-ping CAO Zhi-yan DONG Jin-gao State Key laboratory of North China Crop Improvement and Regulation/Key laboratory of Hebei Province for plant Physiology and Molecular Pathology Baoding 071001P.R.China College of plant Protection Hebei Agricultural UniversityBaoding 071001P.R.China College of Life Sciences Hebei Agricultural UniversityBaoding 071001P.R.China Langfang Academy of Agriculture and Forestry Science Langfang 065000P.R.China

Laccases, as a kind of multicopper oxidase, play an important role in pigment synthesis and growth in fungi and are involved in their interactions with host plants. In Setosphaeria turcica, 9 laccase-like multicopper oxidases have been identified, and StLAC2 is involved in the synthesis of the melanin that accumulates in the cell wall. The function of another major laccase gene, StLAC6, was studied here. The knockout of StLAC6 had no effect on the growth, morphology or invasion ability of S. turcica, but the morphology and function of peroxisomes of knockout mutants were abnormal. The knockout of the StLAC6 gene resulted in increased contents of phenolic compounds and melanin and the sensitivity to fungicides increased compared with wild type strains. In the mutants of StLAC6, there is a significant change of the expression levels of other laccase genes. This study provides a new insight into laccase functions and the relationship of the laccase gene family in plant pathogenic fungi.

关键词： Setosphaeria turcica laccase StLAC6 peroxisome fungicides

Breeding ecology of two sympatric laughingthrushes(Trochalopteron milnei and Garrulax berthemyi)in southwestern China

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Avian Research 2022年第2期13卷 226-228页

作者： Canchao Yang Xiaogang Yao Yan Cai Guangrong Li Wei Liang Ministry of Education Key laboratory for Ecology of Tropical Islands Key Laboratory of Tropical Animal and Plant Ecology of Hainan ProvinceCollege of Life SciencesHainan Normal UniversityHaikou571158China Kuankuoshui National Nature Reserve Suiyang563300China

Here we documented up-to-date information on breeding ecology of Buffy Laughingthrush(Garrulax berthemyi),an endemic species of China,and a sympatric coordinal Red-tailed Laughingthrush(Trochalopteron milnei),in southwestern ***,we compared breeding ecology of these two sympatric *** significant differences were found in clutch size,egg size,nest size,nest height,nest habitat and predation rate between these two species except nests depth,nests cover and eggs *** Red-tailed Laughingthrush nestlings differed from those of the Buffy Laughingthrush in gape morphology and the extent of *** of breeding behavior showed that cooperative breeding might exist in the population of Buffy Laughingthrush.

关键词： Breeding density Cooperative breeding Predation rate Reproduction

Identification and virulence test of a new pathogen that causes verticillium striping on rapeseed in northwestern China

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Oil Crop Science 2024年第1期9卷 8-19页

作者： We Si Ruisheng Wang Mingde Wu Long Yang Guoqing Li Jing Zhang State Key laboratory of Agricultural Microbiology and Key laboratory of plant Pathology of Hubei Province Huazhong Agricultural UniversityWuhan 430070China Qinghai Academy of Agriculture and Forestry Sciences Qinghai UniversityXiningQinghai Province810016China

Five stems of rapeseed with abundant black microsclerotia were collected from Huangyuan County of Qinghai Province,China,and fungal isolates were obtained from the *** were identified based on morphology,molecular features and specific PCR *** results showed that the 10 fungal isolates belonged to Verticillium longisporum lineage A1/*** of the 10 isolates(HW7-1)was tested for virulence on three species of rapeseed,including *** Zhongshuang 9,*** Qingyou 9 and *** Tayou 2 by conidia inoculation of HW7-1 on roots of young *** seedlings were inoculated with *** conidia or water *** seedlings of these treatments were transplanted in culture mix and incubated in a growth chamber(20℃).Results suggested that the control seedlings of three cultivars appeared quite healthy,while the seedlings inoculated with HW7-1 turned yellowing leaves,seedling stunting or even death after 22 days *** was re-isolated from he yellow leaves,thus fulfilling Koch's ***,compared to the control treatments,inoculation with HW7-1 caused flowering delay and seed yield reduction on Tayou 2 with production of microsclerotia on the *** our knowledge,this is the first report of *** lineage A1/D3 on rapeseed in northwestern China.

关键词： Verticillium longisporum lineage A1/D3 Virulence Rapeseed China

Identification and characterization of cDNA sequences encoding the HIS3 and LEU2 genes of the fungus Alternaria tenuissima

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Journal of Genetics and Genomics 2008年第4期35卷 251-256页

作者： Ying Wan Xuli Wang Yun Huang Dewen Qiu Linghuo Jiang State Key laboratory for Biology of plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100094 China Departrnent of plant Protection College of Agricultural Sciences Sichuan Agricultural University Ya＇an 625014 China

Alternaria tenuissima is a fungus widely present in the environment and could cause diseases in plants and humans. In this study, through a yeast genetic approach, cDNA sequences were isolated and characterized for the AtHIS3 and AtLEU2 genes. AtHIS3 cDNA encodes a protein of 238 amino acids, while AtLEU2 cDNA encodes a protein of 363 amino acids. Based on the phylogenetic analysis of amino acid sequences of AtHis3p and AtLeu2p, A. tenuissima is closely related to the plant pathogenic fungus Phaeosphaeria nodorum. This study provides two genetic markers for studies of functions of genes regulating development, morphology, and virulence of A. tenuissima.

关键词： Alternaria tenuissima AtHIS3 AtLEU2 cDNA genetic marker pathogen

Vacuole Integrity Maintained by DUF300 Proteins Is Required for Brassinosteroid Signaling Regulation

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Molecular plant 2018年第4期11卷 553-567页

作者： Qinsong Liu Thomas Vain Corrado Viotti Siamsa M, Doyle Danuse Tarkowska Ondrej Novak Cyril Zipfel Folke Sitbon Stephanie Robert Daniel Hofius Department of plant Biology Uppsala BioCenter Swedish University of Agricultural Sciences (SLU) and Linnean Center for Plant Biology PO Box 7080 750 07 Uppsala Sweden Department of Forest Genetics and plant Physiology Umea Plant Science Centre Swedish University of Agricultural Sciences (SLU) 90183 Umea Sweden Umea plant Science Centre Umea University 90187 Umea Sweden Institute of Biochemistry and Biology Plant Physiology University of Potsdam 14476 Potsdam Germany laboratory of Growth Regulators Centre of the Region Hana for Biotechnological and Agricultural Research institute of Experimental Botany ASCR and Palacky University 783 71 Olomouc Czech Republic The Sainsbury laboratory Norwich Research Park Norwich NR4 7UH UK These authors contributed equally to this article. Present addresses: Key laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education) College of Life Science China West Normal University Nanchong 637009 Sichuan China Institut de Biologie Moleculaire des plantes du CNRS IBMP-CNRS UPR2357 12 rue du General Zimmer 67084 Strasbourg France

Brassinosteroid （BR） hormone signaling controls multiple processes during plant growth and development and is initiated at the plasma membrane through the receptor kinase BRASSINOSTEROID INSENSITIVE1 （BRI1） together with co-receptors such as BRI1-ASSOClATED RECEPTOR KINASE1 （BAK1）. BRI1 abun- dance is regulated by endosomal recycling and vacuolar targeting, but the role of vacuole-related proteins in BR receptor dynamics and BR responses remains elusive. Here, we show that the absence of two DUF300 domain-containing tonoplast proteins, LAZARUS1 （LAZl） and LAZl HOMOLOG1 （LAZlH1）, causes vacuole morphology defects, growth inhibition, and constitutive activation of BR signaling. Intriguingly, tonoplast accumulation of BAK1 was substantially increased and appeared causally linked to enhanced BRI1 trafficking and degradation in lazl lazlhl plants. Since unrelated vacuole mutants exhibited normal BR responses, our findings indicate that DUF300 proteins play distinct roles in the regulation of BR signaling by maintaining vacuole integrity required to balance subcellular BAK1 pools and BR receptor distribution.

关键词： brassinosteroid signaling vacuole integrity DUF300 proteins tonoplast Arabidopsis

Cdk phosphorylation licenses Kif4A chromosome localization required for early mitotic progression

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Journal of Molecular Cell Biology 2018年第4期10卷 358-370页

作者： Zhixiong Dong Changjun Zhu Qimin Zhan Wei Jiang State Key laboratory of Molecular Oncology National Cancer Center/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100021 China Key laboratory of Molecular and Cellular Systems Biology Tianjin Normal University Tianjin 300387 China Tianjin Key laboratory of Animal and plant Resistance College of Life Sciences Tianjin Normal University Tianjin 300387 China

The chromokinesin Kif4A controls proper chromosome condensation, congression/alignment, and cytokinesis to ensure faithful genetic inheritance. Here, we report that Cdk phosphorylation of human Kif4A at T1161 licenses Kif4A chromosomal localization, which, in turn, controls Kif4A early mitotic function. Phosphorylated Kif4A （Kif4A^WT） or Cdk phospho-mimetic Kif4A mutant （Kif4A^TE） associated with chromosomes and condensin I （non-SMC subunit CAP-G and core subunit SMC2） to regulate chromosome condensation, spindle morphology, and chromosome congression/alignment in early mitosis. In contrast, Cdk non-phosphorylatable Kif4A mutant （Kif4A^TA） could neither localize on chromosomes nor associate with CAP-G and SMC2. Furthermore, Kif4A^TA could not rescue defective chromosome condensation, spindle morphology, or chromosome congression/alignment in cells depleted of endogenous Kif4A, which activated a mitotic checkpoint and delayed early mitotic progression. However, targeting Kif4A^TA to chromosomes by fusion of Kif4A^TA with Histone H1 resulted in restoration of chromosome and spindle functions of Kif4A, similar to Kif4A^WT and Kif4A^TE, in cells depleted of endogenous Kif4A. Thus, our results demonstrate that Cdk phosphorylation-licensed chromosomal localization of Kif4A plays a critical role in regulating early mitotic functions of Kif4A that are important for early mitotic progression.

关键词： Kif4A chromokinesin Cdk phosphorylation mitosis

Endocytic Trafficking towards the Vacuole Plays a Key Role in the Auxin Receptor SCF^TIR-Independent Mechanism of Lateral Root Formation in A. thaliana

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Molecular plant 2012年第6期5卷 1195-1209页

作者： Patricio Perez-Henrique Natasha V. Raikhel Lorena Norambuena plant Molecular Biology laboratory Department of Biology Faculty of Sciences University of Chile Santiago Chile Millenium Nucleus in plant Cell Biotechnology Chile Center for plant Cell Biology and Department of Botany and plant Sciences University of California Riverside CA 92521 USA

plants＇ developmental plasticity plays a pivotal role in responding to environmental conditions. One of the most plastic plant organs is the root system. Different environmental stimuli such as nutrients and water deficiency may induce lateral root formation to compensate for a low level of water and/or nutrients. It has been shown that the hor- mone auxin tunes lateral root development and components for its signaling pathway have been identified. Using chemi- cal biology, we discovered an Arabidopsis thaliana lateral root formation mechanism that is independent of the auxin receptor SCFTM. The bioactive compound Sortin2 increased lateral root occurrence by acting upstream from the morpho- logical marker of lateral root primordium formation, the mitotic activity. The compound did not display auxin activity. At the cellular level, Sortin2 accelerated endosomal trafficking, resulting in increased trafficking of plasma membrane recy- cling proteins to the vacuole. Sortin2 affected Late endosome/PVC/MVB trafficking and morphology. Combining Sortin2 with well-known drugs showed that endocytic trafficking of Late E/PVC/MVB towards the vacuole is pivotal for Sortin2- induced SCFTm-independent lateral root initiation. Our results revealed a distinctive role for endosomal trafficking in the promotion of lateral root formation via a process that does not rely on the auxin receptor complex SCFTM.

关键词： lateral root development endocytic trafficking Sortin2 endosomes Arabidopsis.

Characterization and Fine Mapping of Non-panicle Mutant (nop) in Rice

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Rice science 2009年第3期16卷 165-172页

作者： Wu Kun RAO Yu-chun Hu Jiang ZHU Guan-lin ZHANG Guang-hen Hu Xin-ming Guo Long-biao WANG Yong-hong QIAN Qian ZENG Da-li State Key laboratory of Rice Biology China National Rice Research Institute Hangzhou 310006 China State Key laboratory of plant Genomics and National Center for plant Gene Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China Agricultural College Yangzhou University Yangzhou 225009 China

A mutant of panicle differentiation in rice called non-panicle （nop） was discovered in the progeny of a cross between 93-11 and Nipponbare. The mutant exhibits normal plant morphology but has apparently few tillers. The most striking change in nop is that its panicle differentiation is blocked, with masses of fluffy bract nodes generate from the positions where rachis branches normally develop in wild-type plants. Genetic analysis suggests that nop is controlled by a single recessive gene, which is temporarily named Nop（t）. Based on its mutant phenotype, Nop（t） represents a key gene controlling the initiation of inflorescence differentiation, By using simple sequence repeat markers and sequence tagged site markers, Nop（t） gene was fine mapped in a 102-kb interval on the long arm of chromosome 6. These results will facilitate the positional cloning and functional studies of the gene.

关键词： non-panicle gene panicle differentiation fine mapping rice (Oryza sativa)

morphology and Taxonomy of Xiphinema(Nematoda：Longidoridae) Occurring in Arkansas,USA

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江西农业大学学报 2010年第5期32卷 928-945页

作者：叶为民 ROBBINS R.T. Department of plant Pathology Nematology Laboratory2601 N.Young Ave.University of Arkansas Present address:Nematode Assay laboratory North Carolina Department of Agriculture and Consumer Services

In a survey,primarily from the rhizosphere of hardwood trees growing on sandy stream banks,for longidorids,828 soil samples were collected from 37 Arkansas counties in 1999—*** hundred twenty-seven populations of Xiphinema were recovered from 452 of the 828 soil samples(54.6%),including 71 populations of *** sensu lato,33 populations of ***,23 populations of *** and one population of *** morphological and morphometric characteristics of these Arkansas species are *** and morphometric characteristics are also given for two populations of *** from Hawaii and North Carolina.

关键词： Arkansas morphology SEM survey taxonomy Xiphinema americanum X.bakeri X.chambersi X.krugi.