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GreenPhos,a universal method for in-depth measurement of plant phosphoproteomes with high quantitative reproducibility

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Molecular Plant 2024年第1期17卷 199-213页

作者： Xiaoxiao Duan Yuanya Zhang Xiahe Huang Xiao Ma Hui Gao Yan Wang Zhen Xiao Chengcheng Huang Zhongshu Wang Bolong Li Wenqiang Yang Yingchun Wang State Key laboratory of Molecular Developmental Biology Institute of Genetics and Developmental BiologyChinese Academy of SciencesBejing 100101China University of Chinese Academy of Sciences Bejing 100049China Key laboratory of photobiology Instute of BotanyChinese Academy of SciencesBejing 100093China

Protein phosphorylation regulates a variety of important cellular and physiological processes in ***-depth profiling of plant phosphoproteomes has been more technically challenging than that of animal *** is largely due to the need to improve protein extraction efficiency from plant cells,which have a dense cell wall,and to minimize sample loss resulting from the stringent sample clean-up steps required for the removal of a large amount of biomolecules interfering with phosphopeptide purification and mass spectrometry *** this end,we developed a method with a streamlined workflow for highly efficient purification of phosphopeptides from tissues of various green organisms including Arabidopsis,rice,tomato,and Chlamydomonas reinhardtii,enabling in-depth identification with high quantitative reproducibility of about 11000 phosphosites,the greatest depth achieved so far with single liquid chromatography-mass spectrometry(LC-MS)runs operated in a data-dependent acquisition(DDA)*** mainstay features of the method are the minimal sample loss achieved through elimination of sample clean-up before protease digestion and of desalting before phosphopeptide enrichment and hence the dramatic increases of time-and *** method,named GreenPhos,combined with single-shot LC-MS,enabled in-depth quantitative identification of Arabidopsis phosphoproteins,including differentially phosphorylated spliceosomal proteins,at multiple time points during salt stress and a number of kinase substrate *** is expected to serve as a universal method for purification of plant phosphopeptides,which,if samples are further fractionated and analyzed by multiple LC-MS runs,could enable measurement of plant phosphoproteomes with an unprecedented depth using a given mass spectrometry technology.

关键词： plant phosphoproteomics phosphopeptides LC–MS Arabidopsis salt stress

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On breakthroughs and limits: Resolving structural fine-tuning of photosynthetic supercomplexes

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Molecular Plant 2024年第2期17卷 224-226页

作者： Eunchul Kim Marcel Dann Division of Environmental photobiology National Institute for Basic BiologySOKENDAlOkazaki 444-8585Japan Department of Basic Biology The Graduate University for Advanced StudiesSOKENDAlOkazaki 444-8585Japan Bio-Inspired Energy Conversion Technical University of DarmstadtSchnittspahnstraBe 1064287 DarmstadtGermany

Dynamic regulation of photosynthetic supercomplexes is a key strategy allowing plants to adjust their light-harvesting system in response to varying environmental light conditions(Bassi and Dall'Osto,2021).State transition(i.e.,acclimative re-distribution of peripheral antennae between photosystems[PSs]I and II)is one such regulation mechanism that maintains excitation balance between PSl and PSll.

关键词： complexes breakthrough maintain

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Plant photobiology: From basic theoretical research to crop production improvement

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Journal of Integrative Plant Biology 2024年第5期66卷 847-848页

作者： Hongtao Liu Jigang Li College of Life Sciences and Oceanography Shenzhen UniversityShenzhen 518061China State Key laboratory of Plant Environmental Resilience College of Biological SciencesChina Agricultural UniversityBeijing 100193China

As one of the most important environmental signals for plants,light plays a profound role in regulating virtually every aspect of plant growth and *** signals are perceived by plants via several families of photoreceptors,among which phytochromes are responsible for absorbing the red(R)and far-red(FR)wavelengths(600–750 nm),cryptochromes(CRYs)perceive the blue(B)/ultraviolet-A(UV-A)wavelengths(320–500 nm),and UV RESISTANCE LOCUS 8(UVR8)is a recently-characterized UV-B(280–320 nm)photoreceptor.

关键词： UVR8 photoreceptor development

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Green innovation for green light

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Science China(Life Sciences) 2023年第10期66卷 2443-2444页

作者： Junling Huai Rongcheng Lin Key laboratory of photobiology Institute of BotanyChinese Academy of SciencesBeijing 100093China

In darkness,plants undergo skotomorphogenesis,which is characterized by long hypocotyls and closed *** contrast,light,such as far-red light,red light,and blue light,inhibits hypocotyl *** sense different wavelengths o... 详细信息

关键词： light closed

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Regulation of chloroplast protein degradation

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Journal of Genetics and Genomics 2023年第6期50卷 375-384页

作者： Yang Sun Jialong Li Lixin Zhang Rongcheng Lin State Key laboratory of Crop Stress Adaptation and Improvement Schoolof Life SciencesHenan UniversityKaifengHenan 475001China Key laboratory of photobiology Institute of BotanyChinese Academy of SciencesBejing 100093China

Chloroplasts are unique organelles that not only provide sites for photosynthesis and many metabolic processes,but also are sensitive to various environmental *** proteins are encoded by genes from both nuclear and chloroplast *** chloroplast development and responses to stresses,the robust protein quality control systems are essential for regulation of protein homeostasis and the integrity of chloroplast *** this review,we summarize the regulatory mechanisms of chloroplast protein degradation refer to protease system,ubiquitin-proteasome system,and the chloroplast *** mechanisms symbiotically play a vital role in chloroplast development and photosynthesis under both normal or stress conditions.

关键词： Chloroplast Protein homeostasis Protease Ubiquitin-proteasome Chlorophagy

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Autophagy targets Hd1 for vacuolar degradation to regulate rice flowering

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Molecular Plant 2022年第7期15卷 1137-1156页

作者： Zhi Hu Zhipan Yang Yi Zhang Aihong Zhang Qingtao Lu Ying Fang Congming Lu Key laboratory of photobiology Institute of BotanyChinese Academy of SciencesBeijing 100093China University of Chinese Academy of Sciences Beijing 100049China State Key laboratory of Crop Biology College of Life ScienceShandong Agricultural UniversityTaianShandong 271018China

Flowering time(heading date)is a critical agronomic trait that determines the yield and regional adaptability of *** date 1(Hd1)is a central regulator of photoperiodic flowering in rice(Oryza sativa).However,how the homeostasis of Hd1 protein is achieved is poorly ***,we report that the nuclear autophagy pathway mediates Hd1 degradation in the dark to regulate *** of autophagy function results in an accumulation of Hd1 and delays flowering under both short-day and long-day *** the dark,nucleus-localized Hd1 is recognized as a substrate for autophagy and is subjected to vacuolar degradation via the autophagy protein *** Hd1-0sATG8 interaction is required for autophagic degradation of Hd1 in the *** study reveals a new mechanism by which Hd1 protein homeostasis is regulated by autophagy to control rice *** study also indicates that the regulation of flowering by autophagic degradation of Hd1 orthologs may have arisen over the course of mesangiosperm evolution,which would have increased their flexibility and adaptability to the environment by modulating flowering time.

关键词： autophagy heading date rice Hd1 homeostasis

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A new milestone for photosynthesis

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National Science Review 2018年第4期5卷 444-445页

作者： Chunxi Zhang Tingyun Kuang laboratory of Photochemistry Institute of Chemistry Chinese Academy of Sciences laboratory of photobiology Institute of BotanyChinese Academy of Sciences

The photosynthetic water-splitting reaction catalysed by the oxygen-evolving center(OEC)in the photosystem II of plants releases protons,electrons and dioxygen,which is one of the most important processes for energy and material conversions on *** is a Mn4Cacluster and cycles through five different states(Sn,n=0–4),wherein S0is the most reduced state,S1is the dark stable state and S2,S3and S4are intermediate

关键词： OEC A new milestone for photosynthesis

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Cloning, Characterization and Functional Analysis of Two Type 1 Diacylglycerol Acyltransferases (DGAT1s) from Tetraena mongolica

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Journal of Integrative Plant Biology 2013年第6期55卷 490-503页

作者： Minchun Li Mingming Zhao Hanying Wu Wang Wu Yinong Xu Key laboratory of photobiology Institute of Botany the Chinese Academy of Sciences University of Chinese Academy of Sciences

Two cDNAs encoding putative type 1 acyl-CoA： diacylglycerol acyltransferases （DGAT1, EC 2.***.1.20）, were cloned from Tetraena mongolica Maxim., an extreme xerophyte with high oil content in the stems. The 1,488-bp and 1,485-bp of the open reading frame （ORF） of the two cDNAs, designated as TmDGAT1a and TmDGAT1b, were both predicted to encode proteins of 495 and 494 amino acids, respectively. Southern blot analysis revealed that TmDGAT1a and TmDGAT1b both had low copy numbers in the T. mongolica genome. In addition to ubiquitous expression with different intensity in different tissues, including stems, leaves and roots, TmDGAT1a and TmDGAT1b, were found to be strongly induced by high salinity, drought and osmotic stress, resulting in a remarkable increase of triacylglycerol （TAG） accumulation in T. mongolica plantlets. TmDGAT1a and TmDGAT1b activities were confirmed in the yeast H1246 quadruple mutant （DGA1, LRO1, ARE1, ARE2） by restoring DGAT activity of the mutant host to produce TAG. Overexpression of TmDGAT1a and TmDGAT1b in soybean hairy roots as well as in T. mongolica calli both resulted in an increase in oil content （ranging from 37% to 108%）, accompanied by altered fatty acid profiles.

关键词： Diacylglycerol acyltransferase1 Tetraena mongolica triacylglycerol.

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Histology, physiology, and transcriptomic and metabolomic profiling reveal the developmental dynamics of annual shoots in tree peonies (Paeonia suffruticosa Andr.)

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Horticulture Research 2023年第9期10卷 68-84页

作者： Ningning Tong Qingyan Shu Baichen Wang Liping Peng Zheng’an Liu State Key laboratory of Plant Diversity and Specialty Crops Institute of BotanyChinese Academy of SciencesBeijing 100093China China National Botanical Garden Beijing 100093China University of Chinese Academy of Sciences Beijing 100049China Photosynthesis Research Center Key Laboratory of PhotobiologyInstitute of BotanyChinese Academy of SciencesBeijing 100093China

The development of tree peony annual shoots is characterized by“withering”,which is related to whether there are bud points in the leaf axillaries of annual ***,the mechanism of“withering”in tree peony is still *** this study,Paeonia ostii‘Fengdan’and ***‘Luoyanghong’were used to investigate dynamic changes of annual shoots through anatomy,physiology,transcriptome,and *** results demonstrated that the developmental dynamics of annual shoots of the two cultivars were *** withering degree of ***‘Luoyanghong’was higher than that of ***‘Fengdan’,and their upper internodes of annual flowering shoots had a lower degree of lignin deposition,cellulose,C/N ratio,showing no obvious sclerenchyma,than the bottom ones and the whole internodes of vegetative shoot,which resulted in the“withering”of upper internodes.A total of 36 phytohormone metabolites were detected,of which 33 and 31 were detected in ***‘Fengdan’and ***‘Luoyanghong’,*** addition,302 and 240 differentially expressed genes related to lignin biosynthesis,carbon and nitrogen metabolism,plant hormone signal transduction,and zeatin biosynthesis were screened from the two ***,36 structural genes and 40 transcription factors associated with the development of annual shoots were highly co-expressed,and eight hub genes involved in this developmental process were ***,this study explained the developmental dynamic on the varied annual shoots through multi-omics,providing a theoretical foundation for germplasm innovation and the mechanized harvesting of tree peony annual shoots.

关键词： harvesting annual dynamics

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Different B-Type Methionine Sulfoxide Reductases in Chlamydomonas May Protect the Alga against High-Light, Sulfur-Depletion, or Oxidative Stress

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Journal of Integrative Plant Biology 2013年第11期55卷 1054-1068页

作者： Lei Zhao Mei Chen Dongmei Cheng Haomeng Yang Yongle Sun Heyi Zhou Fang Huang Key laboratory of photobiology Institute of Botany the Chinese Academy of Sciences University of Chinese Academy of Sciences

The genome of unicellular green alga Chlamydomonas reinhardtii contains four genes encoding B-type methionine sulfoxide reductases, MSRBI.1, MSRB1.2, MSRB2.1, and MSRB2.2, with functions largely unknown. To understand the cell defense system mediated by the methionine suifoxide reductases in Chlamydomonas, we analyzed expression and physiological roles of the MSRBs under different abiotic stress conditions using immunoblotting and quantitative polymerase chain reaction （PCR） analyses. We showed that the MSRB2.2 protein was accumulated in cells treated with high light （1,300 μE-/m2 per s）, whereas MSRBI.1 was accumulated in the cells under 1 mmol/L H2O2 treatment or sulfur depletion. We observed that the cells with the MSRB2.2 knockdown and overexpression displayed increased and decreased sensitivity to high light, respectively, based on in situ chlorophyll a fluorescence measures. We also observed that the cells with the MSRBI.1 knockdown and overexpression displayed decreased and increased tolerance to sulfur-depletion and oxidative stresses, respectively, based on growth and H2- producing performance. The physiological implications revealed from the experimental data highlight the importance of MSRB2.2 and MSRBI.1 in protecting Chlamydomonas cells against adverse conditions such as high-light, sulfur-depletion, and oxidative stresses.

关键词： Chlamydomonas high light MSRB oxidative stress sulfur deprivation.

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