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A near-infrared fluorescent probe for visualizing transformation pathway of Cys/Hcy and H2S and its applications in living system

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Chinese Chemical Letters 2024年第5期35卷 296-301页

作者： Yudi Cheng Xiao Wang Jiao Chen Zihan Zhang Jiadong Ou Mengyao She Fulin Chen Jianli Li Key laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education Xi’an Key Laboratory of Functional Supramolecular Structure and MaterialsCollege of Chemistry&Materials ScienceNorthwest UniversityXi’an 710127China Key laboratory of Resource Biology and Biotechnology in Western China Ministry of EducationBiomedicine Key Laboratory of Shaanxi ProvinceLab of Tissue Engineeringthe College of Life SciencesFaculty of Life Science&MedicineNorthwest UniversityXi’an 710069China

Sulfydryl-contained(-SH)substances including hydrogen sulfide(H_(2)S),cysteine(Cys),homocysteine(Hcy)and glutathione(GSH)play crucial roles in living systems,and their variations are closely associated with various ***,we developed a near-infrared intramolecular charge transfer(ICT)based fluorescent probe Y-NBD,achieving detection of Cys/Hcy and H_(2)S with different fluorescent signals(green-red for Cys/Hcy,red for H_(2)S),large Stokes shifts(∼100/105nm or 191 nm)and high signal-background-ratio,but not responding to GSH.Y-NBD was successfully applied to image exogenous/endogenous Cys/Hcy and H_(2)S in various living cancer cells(HeLa,A549,and HepG2)and in *** not only visualized the transformation pathway of several thiols in HepG2 cells but also verified that the intestine is the main site for the activation and metabolism of Y-NBD in zebrafish,as well as realized to evaluate the degree of drug-induced liver *** work provides a promising tool for imaging Cys/Hcy and H_(2)S in living systems and shows great potency in evaluating drug-induced liver injury and its treatment.

关键词： Fluorescent probe Near-infrared Hydrogen sulfide Cysteine/homocysteine Large Stokes shift Dicyanoisophorone Drug-induced liver injury

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The structure of TRAF7 coiled-coil trimer provides insight into its function in zebrafish embryonic development

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Journal of Molecular Cell Biology 2024年第1期16卷 43-53页

作者： Xiaozhen Song Ruixing Hu Yi Chen Man Xiao Hong Zhang Shengnan Wu Qing Lu Molecular Diagnostic laboratory Shanghai Children’s HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200040China Bio-X Institutes Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders(Ministry of Education)Shanghai Jiao Tong UniversityShanghai 200030China laboratory of Development and Diseases and State Key laboratory for Medical Genomics Shanghai Institute of HematologyRuĳin HospitalShanghai JiaoTong University School of MedicineShanghai 200025China

TRAF7 serves as a crucial intracellular adaptor and E3 ubiquitin ligase involved in signal transduction pathways,contributing to immune responses,tumor progression,and embryonic *** mutations within the coiled-coil(CC)domain and WD40 repeat domain of TRAF7 could cause brain tumors,while germline pathogenic mutations contribute to severe developmental ***,the precise molecular mechanism underlying TRAF7 involvement in embryonic development remains *** this study,we employed zebrafish as an in vivo model ***7 knock down caused defects in zebrafish embryonic *** determined the crystal structure of TRAF7 CC domain at 3.3Åresolution and found that the CC region trimerization was essential for TRAF7 functionality during zebrafish embryonic ***,disease-causing mutations in TRAF7 CC region could impair the trimer formation,consequently impacting early embryonic development of ***,our study sheds light on the molecular mechanism of TRAF7 CC trimer formation and its pivotal role in embryonic development.

关键词： TRAF7 coiled-coil domain crystal structure trimerization embryonic development

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Advancements in research on transcutaneous electrical acupoint stimulation for alleviating pelvic pain in women

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Gynecology and Obstetrics Clinical Medicine 2024年第1期4卷 65-72页

作者： YI Ling Wang Xin Lei Lu Shang Wang Xiao Hong Chang Rong Zhang Hong Lan Zhu Department of Obstetrics and Gynecology Peking University People's HospitalBeijingChina Peking University Neuroscience research Institute BeijingChina Department of neurobiology Peking University School of Basic Medical SciencesBeijingChina

Background Pelvicpain isacommon complaint of discomfort in women,with an increasing incidence and seriously affecting patients'quality of *** etiology involves multiple systems and disciplines,and therefore treatment modalities are *** electrical acupoint stimulation(TEAS)therapy is a classic analgesic means,and electrical stimulation of local acupoints can *** to discuss the application of TEAS for the treatment of various types of pelvic pain,the selection of optimal parameters and the renewal of *** Result TEAS fortreatmentof pelvic pain in women has been reported mainly for endometriosis,pelvic inflammatory disease(PID),intrauterine adhesion(IUA),pelvic stasis syndrome,interstitial cystitis,primary dysmenorrhoea and other *** for parameter selection,the most used of frequency is dilatational wave,the most used stimulation intensity is 10-20 mA,and the acupoint selection should be based on the specific conditions of *** The analgesic mechanism of TEAS has not been fully elucidated,but the efficacy of the treatment is remarkable and thereare no significant adverse *** selection of the parameters for TEAS and the combinationof acupoints fordifferent pains are stillthe maindirections offutureresearch.

关键词： stimulation pain pelvic

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Aagab is required for zebrafish larval development by regulating neural activity

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Journal of Genetics and Genomics 2024年第6期51卷 630-641页

作者： Shihui Ding Tursunjan Aziz Anming Meng Shunji Jia State Key laboratory of Membrane Biology Tsinghua-Peking Center for Life SciencesSchool of Life SciencesTsinghua UniversityBeijing 100084China Guangzhou laboratory GuangzhouGuangdong 510320China State Key laboratory of Molecular Developmental Biology Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China

Clathrin-mediated endocytosis has been implicated in various physiological processes,including nutrient uptake,signal transduction,synaptic vesicle recycling,maintenance of cell polarity,and antigen *** prior knowledge of its importance as a key regulator in promoting clathrin-mediated endocytosis,the physiological function of α-and γ-adaptin binding protein(aagab)remains *** this study,we investigate the biological function of aagab during zebrafish *** establish a loss-of-function mutant of aagab in zebrafish,revealing impaired swimming and early larval *** the high expression level of aagab in the brain,we probe into its physiological role in the nervous *** mutants display subdued calcium responses and local field potential in the optic tectal neurons,aligning with reduced neurotransmitter release(e.g.,norepinephrine)in the tectal neuropil of aagab *** aagab mRNA or nervous stimulant treatment in mutants restores neurotransmitter release,calcium responses,swimming ability,and ***,our observations show delayed release of FM 1-43 in AAGAB knockdown differentiated neuroblastoma cells,pointing towards a probable link to defective clathrin-mediated synaptic vesicle *** conclusion,our study underscores the significance of Aagab in neurobiology and suggests its potential impacts on neurological disorders.

关键词： Aagab Endocytosis Neural activity Synaptic transmitter release zebrafish

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Modulation of p75 neurotrophin receptor mitigates brain damage following ischemic stroke in mice

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Neural Regeneration research 2024年第10期19卷 2093-2094页

作者： Golnoush Mirzahosseini Tauheed Ishrat Department of Anatomy and neurobiology College of MedicineThe University of Tennessee Health Science CenterMemphisTNUSA Pharmaceutical Sciences College of PharmacyThe University of Tennessee Health Science CenterMemphisTNUSA Neuroscience Institute The University of Tennessee Health Science CenterMemphisTNUSA

Stroke is a significant leading cause of death and disability in the United States(Tsao et al.,2022).Approximately 87% of strokes fall into the ischemic category,mainly caused by arterial blockage(Jayaraj et al.,2019).Although the only FDA-approved effective medication is tissue plasminogen activator(tPA),it should be administrated within 4.5 hours of ischemic ***,tPA has been an integral part of managing acute ischemic stro ke.

关键词： damage arterial death p75

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Slc43a2^(+)T cell metastasis from spleen to brain in RGNNV infected teleost

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Science China(Life Sciences) 2024年第4期67卷 733-744页

作者： Qing Wang Yali Liu Minlin Zhang Min Yang Jiantao Liang Xiaoling Zuo Shaowen Wang Xianze Jia Huihong Zhao Han Jiang Qiang Lin Qiwei Qin College of Marine Sciences South China Agricultural UniversityGuangzhou 510642China CAS Key laboratory of Tropical Marine Bio-Resources and Ecology South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China Nansha-South China Agricultural University Fishery research Institute Guangzhou 511457China Joint University laboratory of Guangdong Province Hong Kong and Marco Region on Marine Bioresource Conservation and ExploitationGuangzhou 510642China University of Chinese Academy of Sciences Beijing 100101China

The origin of T cells in the teleost's brain is *** viewing the central nervous system(CNS)as immune privileged has been widely accepted,previous studies suggest that T cells residing in the thymus but not in the spleen of the teleost play an essential role in communicating with the peripheral ***,we identified nine T cell subpopulations in the thymus and spleen of orange-spotted grouper(Epinephelus coioices)through single-cell RNA-sequencing *** viral CNS infection with red-spotted grouper nervous necrosis virus(RGNNV),the number of slc43a2^(+)T cells synchronously increased in the spleen and *** the infection tests in asplenic zebrafish(tlx1^▲zebrafish model),no increase in the number of slc43a2^(+)T cells was observed in the ***-cell transcriptomic analysis indicated that slc43a2^(+)T cells mature and functionally differentiate within the spleen and then migrate into the brain to trigger an immune *** study suggests a novel route for T cell migration from the spleen to the brain during viral infection in fish.

关键词： central nervous system slc43a2^(+)T cell RGNNV T cell migration teleost

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A polymyxin B loaded hypoxia-responsive liposome with improved biosafety for efficient eradication of bacterial biofilms

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Nano research 2024年第9期17卷 8325-8336页

作者： Fang Liu Lingyun Zou Yongcheng Chen Zuolong Liu Yue Huang Qiao Jin Jian Ji MOE Key laboratory of Macromolecule Synthesis and Functionalization of Ministry of Education Department of Polymer Science and EngineeringZhejiang UniversityHangzhou 310027China State Key laboratory of Transvascular Implantation Devices The Second Affiliated Hospital Zhejiang University School of MedicineHangzhou 310009China

Biofilm-associated bacterial infection brings serious threats to global public health owing to serious antibiotic *** is urgently needed to develop innovative strategies to combat biofilm-associated bacterial *** stand out as the last line of defense against Gram-negative ***,serious nephrotoxicity of polymyxins severely limits their clinical ***,a hypoxia-responsive liposome is designed as the nanocarrier of polymyxin B(PMB)to combat biofilms developed by Gram-negative bacteria.A metronidazole modified lipid(hypoxia-responsive lipid(HRLipid))is synthesized to fabricate hypoxia-responsive liposomes(HRLip).PMB loaded hypoxia-responsive liposomes(HRL-PMB)is then prepared to mitigate the nephrotoxicity of PMB while preserving its excellent bactericidal ***-PMB shows very low hemolysis and cytotoxicity due to liposomal encapsulation of *** can be readily released from HRL-PMB in response to hypoxic biofilm microenvironment,exerting its bactericidal activity to realize biofilm *** excellent in vivo antibiofilm ability of HRL-PMB is confirmed by a Pseudomonas aeruginosa infected zebrafish model and a *** pneumonia infection ***,HRL-PMB can greatly reduce the nephrotoxicity of PMB after intravenous *** hypoxia-sensitive liposomes held great promise to improve the biosafety of highly toxic antibiotics while preserving their intrinsic bactericidal ability,which may provide an innovative strategy for combating biofilm-associated infections.

关键词： bacterial biofilm hypoxia liposome polymyxin B nephrotoxicity

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Differences in pectoral fin spine morphology between vocal and silent clades of catfishes (Order Siluriformes): Ecomorphological implications

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Current Zoology 2010年第1期56卷 73-89页

作者： Ingrid M.KAATZ Donald J.STEWART Aaron N.RJCE Phillip S.LOBEL Department of Environmental and Forest Biology SUNY College of Environmental Science and Forestry Department of neurobiology and behavior Cornell University Bioacoustics research Program Cornell Laboratory of Ornithology Cornell University Department of Biology Boston University

Stridulatory sound-producing behavior is widespread across catfish families, but some are silent. To understand why, we compared spine morphology and ecotype of silent and vocal clades. We determined vocal ability of laboratory specimens during disturbance behavior. Vocal families had bony （not flexible or segmented） spines, well-developed anterior and/or posterior serrations, and statistically significantly longer spines. We compared morphology of the proximal end of the pectoral spine between vocal and silent species. For vocal taxa, microscopic rounded or bladed ridges or knobs were present on the dorsal process. Most silent species had reduced processes with exclusively smooth, convoluted, or honeycombed surfaces very similar to spine-locking surfaces, or they had novel surfaces （beaded, vacuolated, cobwebbed）. Most callichthyids had ridges but many were silent during disturbance. All doradid, most auchenipterid and most mochokid species were vocal and had ridges or knobs. Within the Auchenipteridae, vocal species had spines with greater weight and serration development but not length. Silent auchenipterids had thin, brittle, distally segmented spines with few microscopic serrations on only one margin and a highly reduced dorsal process lacking any known vocal morphology. Silent auchenipterids are derived and pelagic, while all vocal genera are basal and benthopelagic. This is the first phylogenetic evidence for stridulation mechanism loss within catfishes. Phylogenetic mapping of vocal ability, spine condition, and ecotype revealed the repeated presence of silence and vocal taxa, short and long spines, and ecotype shifts within clades. The appearance and loss of vocal behavior and supporting morphologies may have facilitated diversification among catfishes [Current Zoology 56 （1）： 73 89 2010].

关键词： Bioacoustic Defense mechanisms Historical biology Stridulation

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Phase-modulated multi-foci microscopy for rapid 3D imaging

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Photonics research 2024年第7期12卷 1548-1555页

作者： WEIQI WANG LI GONG ZHIWEI HUANG Optical Bioimaging laboratory Department of Biomedical EngineeringCollege of Design and EngineeringNational University of SingaporeSingapore 117576Singapore

3D imaging technology is pivotal in monitoring the functional dynamics and morphological alterations in living cells and ***,conventional volumetric imaging associated with mechanical z-scanning encounters challenges in limited 3D imaging speed with inertial ***,we present a unique phase-modulated multifoci microscopy (PM^(3)) technique to achieve snapshot 3D imaging with the advantages of extended imaging depths and adjustable imaging intervals between each focus in a rapid *** accomplish the tasks,we utilize a spatial light modulator (SLM) to encode the phases of the scattered or fluorescence light emanating from a volumetric sample and then project the multiple-depth images of the sample onto a single charge-coupled device camera for rapid 3D *** demonstrate that the PM^(3)technique provides~55-fold improvement in imaging depth in polystyrene beads phantom compared to the depth of field of the objective lens ***^(3)also enables the real-time monitoring of Brownian motion of fluorescent beads in water at a 15 Hz volume *** precisely manipulating the phase of scattered light on the SLM,PM^(3)can pinpoint the specific depth information in living zebrafish and rapidly observe the 3D dynamic processes of blood flow in the zebrafish *** work shows that the PM^(3)technique developed is robust and versatile for fast 3D dynamic imaging in biological and biomedical systems.

关键词： alterations scattered beads

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Establishment and transcriptome analysis of single blastomerederived cell lines from zebrafish

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Journal of Genetics and Genomics 2024年第9期51卷 957-969页

作者： Jia Xu Siqi Liu Yirui Ai Yunbin Zhang Shifeng Li Yiping Li Key laboratory of Multi-Cell Systems Shanghai Key Laboratory of Molecular AndrologyShanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceUniversity of Chinese Academy of SciencesChinese Academy of SciencesShanghai 200031China

Maintaining chromosome euploidy in zebrafish embryonic cells is challenging because of the degradation of genomic integrity during cell *** this study,we report the derivation of zebrafish cell lines from single *** cell lines have a stable chromosome status attributed to BMP4 and exhibit continuous proliferation in *** zebrafish cell lines are successfully established from single ***-cell transcriptome sequencing analysis confirms the fidelity of gene expression profiles throughout long-term culturing of at least 45 *** long-term cultured cells are specialized into epithelial cells,exhibiting similar expression patterns validated by integrative transcriptomic ***,this work provides a protocol for establishing zebrafish cell lines from single blastomeres,which can serve as valuable tools for in vitro investigations of epithelial cell dynamics in terms of lifeedeath balance and cell fate determination during normal homeostasis.

关键词： zebrafish Single embryo Cell lines Sngle-cell RNA sequencing(scRNA-Seq) Embryonic epidermis Epithelium

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