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Transforming growth factor-beta 1 enhances discharge activity of cortical neurons

Neural Regeneration Research 2025年第2期20卷 548-556页

作者： Zhihui Ren Tian Li Xueer Liu Zelin Zhang Xiaoxuan Chen Weiqiang Chen Kangsheng Li Jiangtao Sheng Department of microbiology and Immunology Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular ImmunopathologyShantou University Medical CollegeShantouGuangdong ProvinceChina Department of Neurosurgery First Affiliated Hospital of Shantou University Medical CollegeShantouGuangdong ProvinceChina

Transforming growth factor-beta 1(TGF-β1)has been extensively studied for its pleiotropic effects on central nervous system *** neuroprotective or neurotoxic effects of TGF-β1 in specific brain areas may depend on the pathological process and cell types ***-gated sodium channels(VGSCs)are essential ion channels for the generation of action potentials in neurons,and are involved in various neuroexcitation-related ***,the effects of TGF-β1 on the functional properties of VGSCs and firing properties in cortical neurons remain *** this study,we investigated the effects of TGF-β1 on VGSC function and firing properties in primary cortical neurons from *** found that TGF-β1 increased VGSC current density in a dose-and time-dependent manner,which was attributable to the upregulation of Nav1.3 *** VGSC current density and Nav1.3 expression were significantly abolished by preincubation with inhibitors of mitogen-activated protein kinase kinase(PD98059),p38 mitogen-activated protein kinase(SB203580),and Jun NH2-terminal kinase 1/2 inhibitor(SP600125).Interestingly,TGF-β1 significantly increased the firing threshold of action potentials but did not change their firing rate in cortical *** findings suggest that TGF-β1 can increase Nav1.3 expression through activation of the ERK1/2-JNK-MAPK pathway,which leads to a decrease in the firing threshold of action potentials in cortical neurons under pathological ***,this contributes to the occurrence and progression of neuroexcitatory-related diseases of the central nervous system.

关键词： central nervous system cortical neurons ERK firing properties JNK Nav1.3 p38 transforming growth factor-beta 1 traumatic brain injury voltage-gated sodium currents

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Determinants of quinolone resistance in Escherichia coli causing community-acquired urinary tract infection in Bejaia,Algeria

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Asian Pacific Journal of Tropical Medicine 2014年第6期7卷 462-467页

作者： Yanat Betitra Vinuesa Teresa Vinas Miguel Touati Abdelaziz Laboratoire d'ecologie Microbiologie.Universite A/Mira de Bejaia laboratory of molecular microbiology and antibiotics Dept.Pathology and Experimental therapeutics.Medical SchoolUniversity of Barcelona

Objective:To investigate the mechanisms of quinolone resistance and the association with other resistance markers among Esherichia coli(***) strains isolated from outpatient with urinary tract infection in north of ***:A total of 30 nalidixic acid-resistant *** isolates from outpatient with urinary tract infections from January 2010 to April 2011 in north of Algeria(Bejaia) were *** susceptibility was determined by disc diffusion assay,minimal inhibitory concentrations(MIC) of quinolone were determined by *** in the Quinolone Resistance-Determining Region(QRDR) of gyra and parC genes and screening for qnr(A,B and S) and bla genes were done by PCK and UNA ***:Most of the *** isolates(56.66%) were shown to carry mutations in gyrA and parC,igyra:Ser83Leu + Asp87Asn and parC:***.16.66 had only an alteration in gyrA:*** isolate produced qnrB-like and two *** isolates were CTXM-15 producers associated with TEM-1 producing in one ***-expression of bla_(LTV,M)_(15) and qnrB was determined in one *** ***:Our findings suggested the community emergence of gyrA and parC alterations and Qnr determinants that contributed to the development and spread of fluoroquinolone resistance in Algerian *** isolates.

关键词： Escherichia coli Community-acquired urinary tract infection QRDR Qnr ESBL

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Screening strategies for quorum sensing inhibitors in combating bacterial infections

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Journal of Pharmaceutical Analysis 2022年第1期12卷 1-14页

作者： Lan Lu Mingxing Li Guojuan Yi Li Liao Qiang Cheng Jie Zhu Bin Zhang Yingying Wang Yong Chen Ming Zeng antibiotics Research and Re-evaluation Key laboratory of Sichuan Province Sichuan Industrial Institute of AntibioticsSchool of PharmacyChengdu UniversityChengdu610000China laboratory of molecular Pharmacology Department of PharmacologySchool of PharmacySouthwest Medical UniversityLuzhou646000SichuanChina

Interference with quorum sensing(QS)represents an antivirulence strategy with a significant promise for the treatment of bacterial infections and a new approach to restoring antibiotic *** the past two decades,a novel series of studies have reported that quorum quenching approaches and the discovery of quorum sensing inhibitors(QSIs)have a strong impact on the discovery of anti-infective drugs against various types of *** discovery of QSI was demonstrated to be an appropriate strategy to expand the anti-infective therapeutic approaches to complement classical antibiotics and antimicrobial *** the discovery of QSIs,diverse approaches exist and develop in-step with the scale of screening as well as specific QS *** review highlights the latest findings in strategies and methodologies for QSI screening,involving activity-based screening with bioassays,chemical methods to seek bacterial QS pathways for QSI discovery,virtual screening for QSI screening,and other potential tools for interpreting QS signaling,which are innovative routes for future efforts to discover additional QSIs to combat bacterial infections.

关键词： Quorum sensing inhibitor Quorum quenching Anti-infective agent Screening strategies

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Evaluating the effectiveness of marine actinobacterial extract and its mediated titanium dioxide nanoparticles in the degradation of azo dyes

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Journal of Environmental Sciences 2014年第4期26卷 775-782页

作者： S Priyaragini S Veena D Swetha L Karthik G Kumar K V Bhaskara Rao molecular and microbiology Research laboratory Environmental Biotechnology Division School of Bio Sciences and Technology VIT University

Aim of the present study was to synthesize titanium dioxide nanoparticles （YiO2 NPs） from marine actinobacteria and to develop an eco-friendly azo-dye degradation method. A total of five actinobacterial isolates were isolated from Chennai marine sediments, Tamilnadu, India and analyzed for the synthesis of TiO2 NPs using titanium hydroxide. Among these, the isolate PSV 3 showed positive results for the synthesis of TiO2 NPs, which was confirmed by UV analysis. Further characterization of the synthesized TiO2 NPs was done using XRD, AFM and FI＇-IR analysis. Actinobacterial crude extract and synthesized TiO2 NPs was found efficient in degrading azo dye such as Acid Red 79 （AR-79） and Acid Red 80 （AR-80）. Degradation percentage was found to be 81% for AR-79, 83% for AR-80 using actinobacterial crude extract and 84% for AR-79, 85% for AR-80 using TiO2 NPs. Immobilized actinobacterial ceils showed 88% for AR-79 and 81% for AR- 80, dye degrading capacity. Degraded components were characterized by FT-IR and GC-MS analysis. The phytotoxicity test with 500 μg/mL of untreated dye showed remarkable phenotypic as well as cellular damage to Tagetes erecta plant. Comparatively no such damage was observed on plants by degraded dye components. In biotoxicity assay, treated dyes showed less toxic effect as compared to the untreated dyes.

关键词： actinobacteria azo dye degradation ZiO2 NPs biotoxicity Tagetes erecta

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Distinct evolution process among type I interferon in mammals

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Protein & Cell 2013年第5期4卷 383-392页

作者： Lei Xu Limin Yang Wenjun Liu CAS Key laboratory of Pathogenic microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China China-Japan Joint laboratory of molecular Immunology and molecular microbiology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China

Interferon (IFN) is thought to play an important role in the vertebrate immune system, but systemic knowledge of IFN evolution has yet to be elucidated. To evaluate the phylogenic distribution and evolutionary history of type I IFNs, 13genomes were searched using BLASTn program, and a phylogenetic tree of vertebrate type I IFNs was constructed. In the present study, an IFNδ-like gene in the human genome was identified, refuting the concept that humans have no IFNδ genes, and other mammalian IFN genes were also identified. In the phylogenetic tree, the mammalian IFNβ, IFNɛ, and IFNκ formed a clade separate from the other mammalian type I IFNs, while piscine and avian IFNs formed distinct clades. Based on this phylogenetic analysis and the various characteristics of type I IFNs, the evolutionary history of type I IFNs was further evaluated. Our data indicate that an ancestral IFNα-like gene forms a core from which new IFNs divided during vertebrate evolution. In addition, the data suggest how the other type I IFNs evolved from IFNα and shaped the complex type I IFN system. The promoters of type I IFNs were conserved among different mammals, as well as their genic regions. However, the intergenic regions of type I IFN clusters were not conserved among different mammals, demonstrating a high selection pressure upon type I IFNs during their evolution.

关键词： type I IFN evolutionary history vertebrate gene cluster

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The chemokines CXCL8 and CXCL12:molecular and functional properties,role in disease and efforts towards pharmacological intervention

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Cellular & molecular Immunology 2023年第3期20卷 217-251页

作者： Seppe Cambier Mieke Gouwy Paul Proost laboratory of molecular Immunology Rega InstituteDepartment of MicrobiologyImmunology and TransplantationKU LeuvenLeuvenBelgium

Chemokines are an indispensable component of our immune system through the regulation of directional migration and activation of ***8 is the most potent human neutrophil-attracting chemokine and plays crucial roles in the response to infection and tissue ***8 activity inherently depends on interaction with the human CXC chemokine receptors CXCR1 and CXCR2,the atypical chemokine receptor ACKR1,and ***,(hetero)dimerization and tight regulation of transcription and translation,as well as post-translational modifications further fine-tune the spatial and temporal activity of CXCL8 in the context of inflammatory diseases and *** CxCL8 interaction with receptors and glycosaminoglycans is therefore a promising target for therapy,as illustrated by multiple ongoing clinical ***8-mediated neutrophil mobilization to blood is directly opposed by CXCL12,which retains leukocytes in bone ***12 is primarily a homeostatic chemokine that induces migration and activation of hematopoietic progenitor cells,endothelial cells,and several leukocytes through interaction with CXCR4,ACKR1,and ***,it is an essential player in the regulation of embryogenesis,hematopoiesis,and ***,CXCL12 can also exert inflammatory functions,as illustrated by its pivotal role in a growing list of pathologies and its synergy with CXCL8 and other chemokines to induce leukocyte ***,we review the plethora of information on the CXCL8 structure,interaction with receptors and glycosaminoglycans,different levels of activity regulation,role in homeostasis and disease,and therapeutic ***,we discuss recent research on CXCL12 biochemistry and biology and its role in pathology and pharmacology.

关键词： CXCL8 CXCL12 atypical chemokine receptor GPCR glycosaminoglycan

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In silico characterization of the functional and structural modules of the hemagglutinin protein from the swine-origin influenza virus A (H1N1)-2009

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Science China(Life Sciences) 2010年第6期53卷 633-642页

作者： Christopher VAVRICKA GAO George F CAS Key laboratory of Pathogenic microbiology and Immunology Institute of MicrobiologyChinese Academy of Sciences Graduate University of Chinese Academy of Sciences China-Japan Joint laboratory of molecular Immunology and molecular microbiology Institute of MicrobiologyChinese Academy of Sciences Beijing Institutes of Life Science Chinese Academy of Sciences

The 2009 swine-origin influenza virus (S-OIV,H1N1 subtype) has developed into a new pandemic influenza as announced by the World Health *** order to uncover clues about the determinants for virulence and pathogenicity of the virus,we characterized the functional modules of the surface glycoprotein hemagglutinin (HA),the most important protein in molecular epidemiology and pathogenesis of influenza *** analyzed receptor binding sites,basic patch,neutralization antibody epitopes and T cell epitopes in the HA protein of the current S-OIV according to the corresponding functional and structural modules previously characterized in other H1 HA molecules or HA molecules of other *** compared their differences and similarities *** on the amino acids defined as the functional and structural modules,the HA protein of 2009 S-OIV should specifically bind to the human 2,*** D225G/E mutation in HA,which is found in some isolates,may confer dual binding specificity to the 2,3and 2,6-receptor based on previously reported *** HA variant contains two basic patches,one of which results in increased basicity,suggesting enhanced membrane fusion *** 2009 S-OIV HA also has an extra glycosylation site at position *** of the five antibody neutralization epitopes identified in A/RP/8/34(H1N1) were exposed,but the other was hidden by a glycosylation *** previously identified cytotoxic T cell epitopes in various HA molecules were summarized and their corresponding sequences in 2009 S-OIV HA were *** results are critical for understanding the pathogenicity of the virus and host immune response against the virus.

关键词： 2009 S-OIV hemagglutinin in silico structural and functional modules

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Website for avian flu information and bioinformatics

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Science China(Life Sciences) 2009年第5期52卷 470-473页

作者： GAO George Fu CAS Key laboratory of Pathogenic microbiology and Immunology (CASPMI) Institute of Microbiology Chinese Academy of Sciences (CAS) China-Japan Joint laboratory on molecular microbiology and molecular Immunology Institute of Microbiology Chinese Academy of Sciences Beijing Institutes of Life Science (BioLS) Chinese Academy of Sciences

Highly pathogenic influenza A virus H5N1 has spread out worldwide and raised the public concerns. This increased the output of influenza virus sequence data as well as the research publication and other reports. In order to fight against H5N1 avian flu in a comprehensive way, we designed and started to set up the Website for Avian Flu Information (http://***) from 2004. Other than the influenza virus database available, the website is aiming to integrate diversified information for both researchers and the public. From 2004 to 2009, we collected information from all aspects, i.e. reports of outbreaks, scientific publications and editorials, policies for prevention, medicines and vaccines, clinic and diagnosis. Except for publications, all information is in Chinese. Till April 15, 2009, the cumulative news entries had been over 2000 and research papers were approaching 5000. By using the curated data from Influenza Virus Resource, we have set up an influenza virus sequence database and a bioinformatic platform, providing the basic functions for the sequence analysis of influenza virus. We will focus on the collection of experimental data and results as well as the integration of the data from the geological information system and avian influenza epidemiology.

关键词： avian influenza virus website bioinformatic platform

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Stable non-synonymous substitutions on NS gene (NS1 and NS2 proteins) of Qinghai lake H5N1 influenza virus (Clade 2.2) after successive passages in Muscovy ducks

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Science China(Life Sciences) 2009年第9期52卷 847-853页

作者： GAO George F. Key laboratory of Pathogenic microbiology and Immunology of CAS (CASPMI) Institute of Microbiology Chinese Academy of Sciences (CAS) China-Japan Joint laboratory of molecular Immunology and molecular microbiology Institute of Microbiology Chinese Academy of Sciences Graduate University of Chinese Academy of Sciences Beijing Institutes of Life Science Chinese Academy of Sciences

Although worldwide concern has been raised since the large-scale outbreak of highly pathogenic avian influenza in wild birds at Qinghai Lake,China in 2005,the factors responsible for the ability to kill waterfowl remain unclear. The why and how questions of the H5N1 virus species-jump into its reservoir host need to be answered. In this report we test the pathogenicity and adaptation of Qinghai Lake (Clade 2.2) isolate to Muscovy ducks for further understanding of this virus. The isolate was highly pathogenic in ducks and retained its high pathogenicity even after 20 generations of passage in ducks. During the process of serial passages,only the NS gene developed non-synonymous substitutions,which caused two mutations in NS1 protein (Val23Ala and Leu207Pro) and one in NS2 (Phe55Leu). These mutations persisted immutably through all subsequent passages and the pathogenicity remained high,implying that highly pathogenic H5N1 virus remains stable in aquatic birds through oral transmission. Although the exact functions of these mutations are not known,our results provide an important foundation for further understanding the characteristics of the Qinghai Lake isolates.

关键词： avian influenza virus H5N1 mutation NS1/2 serial passages

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Chemoattractants and cytokines in primary ciliary dyskinesia and cystic fibrosis: key players in chronic respiratory diseases

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Cellular & molecular Immunology 2018年第4期15卷 312-323页

作者： Maaike Cockx Mieke Gouwy Jo Van Damme Sofie Struyf laboratory of molecular Immunology Department of Microbiology and ImmunologyRega Institute for Medical ResearchUniversity of LeuvenLeuven 3000Belgium

Patients with primary ciliary dyskinesia(PCD)and cystic fibrosis(CF),two inherited disorders,suffer from recurrent airway infections characterized by persistent bacterial colonization and uncontrollable *** present in high counts,neutrophils fail to clear infection in the *** levels of C-X-C motif chemokine ligand 8/interleukin-8(CXCL8/IL-8),the most potent chemokine to attract neutrophils to sites of infection,are detected in the sputum of both patient groups and might cause the high neutrophil influx in the ***,in CF,airway neutrophils are highly activated because of the genetic defect and the high levels of proinflammatory chemoattractants and cytokines(e.g.,CXCL8/IL-8,tumor necrosis factor-αand IL-17).The overactive state of neutrophils leads to lung damage and fuels the vicious circle of infection,excessive inflammation and tissue *** inflammatory process in CF airways is well characterized,whereas the lung pathology in PCD is far less *** knowledge of CF lung pathology could be useful to guide molecular investigations of the inflammatory processes in PCD *** available therapies can not completely remedy the chronic airway infections in these *** review gives an overview of the role that chemoattractants and cytokines play in these neutrophil-dominated lung ***,the most frequently applied treatments in CF and PCD and new experimental therapies to reduce neutrophil-dominated airway inflammation are described.

关键词： chemoattractants cystic fibrosis cytokines primary ciliary dyskinesia

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