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molecular cloning and expression patterns of the cholesterol side chain cleavage enzyme(CYP11A1) gene during the reproductive cycle in goose(Anas cygnoides)

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Journal of Animal Science and Biotechnology 2016年第2期7卷 141-148页

作者： Qi Xu Yadong Song Yang Chen Ran Liu Yang Zhang Yang Li Zhengyang Huang Wenming Zhao Guobin Chang Guohong Chen Key laboratory of Animal Genetics and Breeding and molecular Design of Jiangsu Province Yangzhou University

Background： CYP11A1, a gene belonging to the family 11 of cytochrome P450, encodes a crucial steroidogenic enzyme that catalyzes the initial step in the production of all classes of steroids. Many studies show that CYP11A1 plays a role in ovary function. However, the role of CYP11A1 in goose reproductive cycle remains largely ***： In this study, full-length CYP11A1 c DNA of Zhedong goose was obtained using reverse transcription polymerase chain reaction（RT-PCR） and rapid amplification of c DNA ends（RACE）. The c DNA consisted of a 96-base pair（bp） 5′untranslated region（UTR）, a 179-bp 3′UTR and a 1509-bp open reading frame. The open reading frame encodes a putative 503 amino acid protein that shares high homology with CYP11A1 of other birds. The amino acid sequence possesses conserved domains of the P450 superfamily, which include the steroid-binding domain and the heme-binding region. Real-time quantitative polymerase chain reaction（q PCR） analysis revealed CYP11A1 mR NA was expressed ubiquitously in every Zhedong goose tissue analyzed, including the heart, liver, glandular stomach,lung, spleen, kidney, intestinum tenue, intestinum crassum, cerebrum, cerebellum, muscle, oviduct, pituitary,hypothalamus and ovary.. The relatively low levels of CYP11A1 m RNA were detected in pituitary, ovary and oviduct tissues at ovulation when compared with levels at oviposition. Interestingly, higher expression was observed in ovary and oviduct tissues during brooding. Lastly, higher m RNA expression of Yangzhou geese was detected during the ovulation period than that of Zhedong ***： Our findings reveal the sequence characterization and expression patterns of the CYP11A1 gene during the goose reproductive cycle, which may provides correlative evidence that CYP11A1 expression is important in reproduction activity.

关键词： CYP11A1 Gene expression Goose Reproduction

Optical molecular Imaging Frontiers in Oncology: The Pursuit of Accuracy and Sensitivity

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Engineering 2015年第3期1卷 309-323页

作者： Kun Wang Chongwei Chi Zhenhua Hu Muhan Liu Hui Hui Wenting Shang Dong Peng Shuang Zhang Jiezuo Ye Haixiao Liu Jie Tian Key laboratory of molecular Imaging Institute of Automation Chinese Academy of Sciences

Cutting-edge technologies in optical molecular imaging have ushered in new frontiers in cancer research, clinical translation, and medical practice, as evidenced by recent advances in optical multimodality imaging, Cerenkov luminescence imaging(CLI), and optical imageguided surgeries. New abilities allow in vivo cancer imaging with sensitivity and accuracy that are unprecedented in conventional imaging approaches. The visualization of cellular and molecular behaviors and events within tumors in living subjects is improving our deeper understanding of tumors at a systems level. These advances are being rapidly used to acquire tumor-to-tumor molecular heterogeneity, both dynamically and quantitatively, as well as to achieve more effective therapeutic interventions with the assistance of real-time imaging. In the era of molecular imaging, optical technologies hold great promise to facilitate the development of highly sensitive cancer diagnoses as well as personalized patient treatment—one of the ultimate goals of precision medicine.

关键词： optical molecular imaging multimodality molecular imaging optical multimodality tomography Cerenkov luminescence imaging intraoperative image-guided surgery

Mechanisms underlying key agronomic traits and implications for molecular breeding in soybean

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Journal of Genetics and Genomics 2024年第4期51卷 379-393页

作者： Chao Fang Haiping Du Lingshuang Wang Baohui Liu Fanjiang Kong Guangdong Key laboratory of Plant Adaptation and molecular Design Guangzhou Key Laboratory of Crop Gene EditingInnovative Center of Molecular Genetics and EvolutionSchool of Life SciencesGuangzhou UniversityGuangzhouGuangdong 510006China

Soybean(Glycine max[L.]Merr.)is an important crop that provides protein and vegetable oil for human *** soybean is a photoperiod-sensitive crop,its cultivation and yield are limited by the photoperiodic conditions in the *** contrast to other major crops,soybean has a special plant architecture and a special symbiotic nitrogen fixation system,representing two unique breeding ***,flowering time,plant architecture,and symbiotic nitrogen fixation are three critical or unique yielddetermining *** review summarizes the progress made in our understanding of these three critical yield-determining factors in ***,we propose potential research directions to increase soybean production,discuss the application of genomics and genomic-assisted breeding,and explore research directions to address future challenges,particularly those posed by global climate changes.

关键词： Soybean Grain yield Flowering time Plant architecture Nodulation Symbiotic nitrogen fixation Genome study

Theoretical study of the low-lying electronic excited states for molecular aggregates

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Science China Chemistry 2013年第9期56卷 1258-1262页

作者： SHUAI ZhiGang LIU WenJian LIANG WanZhen SHI Qiang CHEN Hui MOE Key laboratory of Organic Optoelectronics and molecular Engineering Department of ChemistryTsinghua University Beijing National laboratory for molecular Sciences (BNLMS) Institute of Theoretical and Computational ChemistryState Key Laboratory of Rare Earth Materials Chemistry and ApplicationsCollege of Chemistry and Molecular Engineeringand Center for Computational Science and EngineeringPeking University Department of Chemistry Xiamen University Beijing National laboratory for molecular Science (BNLMS) Institute of ChemistryChinese Academy of Sciences

We present here a brief summary of a National Natural Science Foundation Major Project entitled "Theoretical study of the low-lying electronic excited state for molecular aggregates". The project focuses on theoretical investigation of the electronic structures and dynamic processes upon photo-and electric-excitation for molecules and aggregates. We aim to develop reliable methodology to predict the optoelectronic properties of molecular materials related to the electronic excitations and to apply in the experiments. We identify two essential scientific challenges: (i) nature of intramolecular and intermolecular electronic excited states; (ii) theoretical description of the dynamic processes of the coupled motion of electronic excitations and nucleus. We propose the following four subjects of research: (i) linear scaling time-dependent density-functional theory and its application to open shell system; (ii) computational method development of electronic excited state for molecular aggregates; (iii) theoretical investigation of the time evolution of the excited state dynamics; (iv) methods to predict the optoelectronic properties starting from electronic excited state investigation for organic materials and experimental verifications.

关键词： linear scaling TDDFT relativistic TDDFT excited-state dynamics theory molecular aggregates opto-electronic properties for organic materials charge mobility and exciton diffusion

Experiencing winter for spring flowering: A molecular epigenetic perspective on vernalization

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Journal of Integrative Plant Biology 2020年第1期62卷 104-117页

作者： Xiao Luo Yuehui He National Key laboratory of Plant molecular Genetics & Shanghai Center for Plant Stress Biology Center for Excellence in Molecular Plant Sciences The Chinese Academy of Sciences

Many over-wintering plants, through vernalization, overcome a block to flowering and thus acquire competence to flower in the following spring after experiencing prolonged cold exposure or winter cold. The vernalization pathways in different angiosperm lineages appear to have convergently evolved to adapt to temperate climates. molecular and epigenetic mechanisms for vernalization regulation have been well studied in the crucifer model plant Arabidopsis ***, we review recent progresses on the vernalization pathway in Arabidopsis. In addition, we summarize current molecular and genetic understandings of vernalization regulation in temperate grasses including wheat and Brachypodium, two monocots from Pooideae, followed by a brief discussion on divergence of the vernalization pathways between Brassicaceae and Pooideae.

关键词： winter spring temperate

molecular-based conducting magnet

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Science China Chemistry 2012年第6期55卷 883-892页

作者： ZHANG Bin ZHU DaoBen Beijing National laboratory of molecular Science Center of Molecular Science Organic Solid Laboratory Institute of Chemistry Chinese Academy of Sciences Beijing 100190 China

molecular-based conducting magnet or magnetic conductor, is an overlap of organic conductor and molecular magnet. Due to the existence of ferromagnetism, antiferromagnetism and quantum magnetism in insulated charge-transfer salt, it becomes a common sense that magnetism is not good for conductivity. After the discovery of first molecular-based metallic ferromagnet, molecular-based conducting magnet with n-unit from organic conductor and magnetism from coordination counterion became a hot area. The metallic ferromagnet, semiconductor room-temperature ferrimagnet, metallic weak ferromagnet and supercon- ducting antiferromagnet have been discovered. The new molecular-based conducting magnet with higher conductivity and higher magnetic ordering temperature is expected.

关键词： molecular magnet molecular conductor dual-function molecular crystal conductivity magnetism

molecular dynamics study of the nucleation behavior and lamellar mergence in polyethylene globule crystallization

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Science China Chemistry 2013年第2期56卷 195-202页

作者： WANG SiMiao YU Xiang KONG Bin YANG XiaoZhen Beijing National laboratory for molecular Sciences State Key Laboratory of Polymer Science and Engineering Joint Laboratory of Polymer Science and Materials Institute of Chemistry Chinese Academy of Sciences

The site order parameter (SOP) has been adopted to analyze various orders of structure formation and distribution during the crystallization of a polyethylene globule simulated by molecular dynamics. The SOP not only identifies different orders among the structures but also different phases. The results showed that intermediate structures with medium order exist unevenly in the early stage of nucleation, which differs from traditional models. We found that the nucleation relies on crystallinity fluctuations with increasing amplitude, and a baby nucleus with different shape and increasing size suddenly appears in the fluctuations. Once its size is large enough, the baby nucleus becomes the nucleus. In the growth stage, a number of lamellar mergences were observed and their selective behaviors were related to the orientation differences between the lamellae to be merged. The SOP distribution of all atoms in the system during crystallization had two peaks: one for the amorphous phase and the other for the crystalline phase. The intermediate structures with medium orders are located between the two peaks as an order promotion pathway. The data show that the medium order structure fluctuates at the growth front and is not always available, and that the medium order structure existing at the front is not always good for development. It is possibly caused by chain entanglement. Structure fluctuation at the growth front enables the system showing thousands of the most probable configurations to approach the precursor. The growth front is thus active for a while and inactive at other times.

关键词： molecular dynamics nucleation behavior lamellar mergence polyethylene globule crystallization site order parameter

molecular cloning and characterization of a cotton phosphoenolpyruvate carboxylase gene

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Progress in Natural Science:Materials International 2008年第5期18卷 539-545页

作者： Zhixin Qiao, Jin-Yuan Liu laboratory of molecular Biology and Protein Science laboratory of the Ministry of Education, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China laboratory of molecular Biology and Protein Science laboratory of the Ministry of Education Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China

Phosphoenolpyruvate carboxylase (PEPC) plays diverse physiological functions during plant development. In this study, a new phos- phoenolpyruvate carboxylase gene GhPEPC2 is isolated from cotton (Gossypium hirsutum cv. zhongmian 35) by RACE-PCR. The cloned cDNA of GhPEPC2 is 3364 bp in length, and has an open reading frame of 2913 bp, encoding for 971 putative amino acids with a calculated molecular mass of 110.6 kD and pI of 5.56. The deduced amino acid sequence of GhPEPC2 shares high similarity with other reported plant PEPCs. Southern blot analysis indicates that the cotton PEPC exists as a small gene family and the GhPEPC2 might have two copies in the cotton genome. The semi-quantitative RT-PCR reveals that GhPEPC2 constitutively expresses in all the tissues of cot- ton and accumulated highly in roots, owers and embryos but relatively low in stems and bers. In addition, the recombinant GhPEPC2 has been puri ed by expressing it in Escherichia coli and the catalytic properties of it were also investigated. The results showed that GhPEPC2 is a typical C3 PEPC with a higher Km (83.6 lM) and lower Vmax (8.0 lmol min 1 mg 1) compared with the C3 PEPCs pre- viously reported.

关键词： Phosphoenolpyruvate carboxylase Recombinant protein Southern blot Cotton

Application of machine learning method in optical molecular imaging: a review

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Science China(Information Sciences) 2020年第1期63卷 5-20页

作者： Yu AN Hui MENG Yuan GAO Tong TONG Chong ZHANG Kun WANG Jie TIAN CAS Key laboratory of molecular Imaging Beijing Key Laboratory of Molecular ImagingThe State Key Laboratory of Management and Control for Complex SystemsInstitute of Automation Chinese Academy of Sciences Beijing Advanced Innovation Center for Big Data-Based Precision Medicine School of MedicineBeihang University University of Chinese Academy of Sciences

Optical molecular imaging(OMI) is an imaging technology that uses an optical signal, such as near-infrared light, to detect biological tissue in organisms. Because of its specific and sensitive imaging performance, it is applied in both preclinical research and clinical surgery. However, it requires heavy data analysis and a complex mathematical model of tomographic imaging. In recent years, machine learning(ML)-based artificial intelligence has been used in different fields because of its ability to perform powerful data processing. Its analytical capability for processing complex and large data provides a feasible scheme for the requirement of OMI. In this paper, we review ML-based methods applied in different OMI modalities.

关键词： optical molecular imaging machine learning artificial intelligence