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Open Journal of Veterinary Medicine 2017年第4期7卷 38-47页

作者： Edgar G. Valdivia Lara Andrés ángeles Aldana Jesica I. ángeles Solis Patricia Miranda Castro Juan C. Del Río García Guillermo Valdivia Anda laboratory of microbial pathogenicity Multidisciplinary Research Unit Faculty of Higher Education Cuautitlán National Autonomous University of Mexico Cuautitlán Izcalli Mexico laboratory DIVETTM Cuautitlan Izcalli Mexico Biotechnology laboratory UNAM Cuautitlan Izcalli Mexico laboratory of Mycotoxins Multidisciplinary Research Unit Faculty of Higher Education Cuautitlan National Autonomous University of Mexico Cuautitlan Izcalli Mexico

Neutrophils are the most important circulating phagocytes. Circulating mono-cytes and precursors of tissue macrophages also have the ability to phagocytize. Pidotimod (ADIMODTM) exerts immunostimulatory and immunoregulatory effects through the stimulation and regulation of cellular immune responses by lymphocytes Canine herpesvirus (CHV) mainly affect puppies between the first and second weeks of age, causing high morbidity in the litter. To date, there is only one commercial vaccine in Europe to prevent disease. In this work, inactivated CHV cultures were inoculated in rabbits, adsorbed and not adsorbed to chitosan nanoparticles. Phagocytosis in the presence or absence of specific antibodies was measured. Response of virus neutralizing antibodies was also evaluated. AdimodTM enhanced the nonspecific and specific phagocytotic response. The association of the virus to the nanoparticles increased the phagocytic ability of blood cells;however, AdimodTM alone had a greater effect on phagocytic activity and generated a stronger immune response that corresponded to the increased phagocytosis (p TM was used.

关键词： Phagocytosis Canine Herpesvirus Pidotimod Stimulation

Regulation of eight avr genes by hrpG and hrpX in Xanthomonas campestris pv. campestris and their role in pathogenicity

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Progress in Natural Science:Materials International 2006年第12期16卷 1288-1294页

作者： XU Rongqi~ 1,3 , LI Xianzhen~3, WEI Hongyu~3, JIANG Bole~2, LI Kai~3, HE Yongqiang~ 2,3 , FENG Jiaxun~ 1,3 and TANG Jiliang~ 1,2 **(1. Guangxi Key laboratory of Subtropical Bioresources Conservation and Utilization 2. The Key laboratory of Ministry of Education for microbial and Plant Genetic Engineering 3. College of Life Science and Technology, Guangxi University, Nanning 530004, China) Guangxi Key laboratory of Subtropical Bioresources Conservation and Utilization Guangxi University Nanning 530004 China College of Life Science and Technology Guangxi University Nanning 530004 China The Key laboratory of Ministry of Education for microbial and Plant Genetic Engineering Guangxi University Nanning 530004 China

Eight putative avirulence genes in Xanthomonas campestris pv. campestris (Xcc) strain 8004 were characterized by Tn5gusA5 mutagenesis and gene expression analysis. The virulence test of mutants on Chinese radish showed that all mutants in individual avr genes except avrBs2 mutant were not significantly different from the wild type in virulence. The avrBs2 mutant showed reduced virulence and bacterial growth in planta. Gene expression analysis using β-glucuronidase as reporter indicated that avrBs1.1,avrBs1,avrXccB,avrXccC,avrXccE1 were regulated by hrpG, whereas avrXccA1, avrXccA2 and avrBs2 were not. RT-PCR analysis showed that all hrpG-regulated genes except avrBs1 were also regulated by hrpX. In addition, it was demonstrated that avrBs1 was responsible for elicitation of a type III dependent hypersensitive reaction (HR) on nonhost plant pepper ECW-10R, and wild type Xcc 8004 was unable to cause HR on pepper ECW-20R.

关键词： Xanthomonas campestris pv. campestris avirulence gene virulence expression analysis.

Evaluation of Beauveria bassiana （Hyphomycetes） isolates as potential agents for control of Dendroctonus valens

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Insect Science 2011年第2期18卷 209-216页

作者： Long-Wa Zhang Yu-Jun Liu Jian Yao Bin Wang Bo Huang Zeng-Zhi Li Mei-Zhen Fan Jiang-Hua Sun Anhui Provincial Key laboratory of microbial Pest Control Anhui Agricultural University Heifei Anhui Entry-Exit Inspection and Quaran- tine Bureau Hefei State Key laboratory of Integrated Management Agricultural Pest & Rodents Institute of Zoology Chinese Academy of Sciences Beijing China

The red turpentine beetle （RTB）, Dendroctonus valens LeConte, as a destructive invasive pest, has become one of the most economically important forest pest in China. Effective control measures are desperately needed. Entomopathogenic fungi, such as Beauveria bassiana, have shown great potential for the management of some bark beetle species. In this study, 12 isolates of B. bassiana from bark beetle were examined for biological characteristics and virulence, to assess their potential as biocontrol agents for RTB. There were significant differences （at P = 0.05） in colony growth rate, conidial yield, conidial germination, tolerance to UV light and extracellular proteases activity among the tested B. bassiana isolates. Isolates, including Bbl801, Bb1906, Bb789 and Bb773, exhibited the best characteristics, because they have faster hyphal growth rate, higher spore production and faster spore germination, higher UV tolerance and protease （Prl） production. The results of a pathogenicity test ofB. bassiana on RTB larvae showed that most isolates of B. bassiana have demonstrated high efficacy and the highest virulent isolate was Bb 1801, which killed 100% of the treated insects and had a median lethal time （LT50） of 4.60 days at a concentration of 1 ×107 conidia/mL. Therefore, isolate Bb1801 has a great potential for sustainable control of RTB in the forest. The correlation between biological characteristics and virulence of the fungal isolates is discussed and the possibility of combination of entomopathogenic fungi with semiochemicals, as one of the promising strategy for RTB control, is considered.

关键词： Beauveria bassiana biological characteristics microbial control Dendroctonus valens virulence

Chaetocin reactivates the lytic replication of Epstein-Barr virus from latency via reactive oxygen species

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Science China(Life Sciences) 2017年第1期60卷 66-71页

作者： Shilun Zhang Juan Yin Jiang Zhong Department of Microbiology and microbial Engineering School of Life Sciences Fudan University Shanghai 200438 China

Oxidative stress, regarded as a negative effect of free radicals in vivo, takes place when organisms suffer from harmful stimuli. Some viruses can induce the release of reactive oxygen species （ROS） in infected cells, which may be closely related with their pathogenicity. In this report, chaetocin, a fimgal metabolite reported to have antimicrobial and cytostatic activity, was studied for its effect on the activation of latent Epstein-Barr virus （EBV） in B95-8 cells. We found that chaetocin remarkably up-regulated EBV lytic transcription and DNA replication at a low concentration （50 nmol L-l）. The activation of latent EBV was accompanied by an increased cellular ROS level. N-acetyl-L-cysteine （NAC）, an ROS inhibitor, suppressed chaetocin-induced EBV activation. Chaetocin had little effect on histone H3K9 methylation, while NAC also significantly reduced H3K9 methylation. These results suggested that chaetocin reactivates latent EBV primarily via ROS pathways.

关键词： Epstein-Barr virus reactive oxygen species chaetocin

PKC-SWI6 signaling regulates asexual development,cell wall integrity,stress response,and lifestyle transition in the nematode-trapping fungus Arthrobotrys oligospora

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Science China(Life Sciences) 2022年第12期65卷 2455-2471页

作者： Meihua Xie Ni Ma Na Bai Le Yang Xuewei Yang Ke-Qin Zhang Jinkui Yang State Key laboratory for Conservation and Uilization of Bio-Resources Key Laboratory for Microbial Resources of the Ministry of EducationSchool of Life SciencesYumman UniversityKunming 650091China School of Resource Emvironment and ChemistryChuciong Normal UniversityChnuciong 675000China Yunnan Center for Disease Control and Prevention Kunming 650022China

Predatory fungi possess intricate signal transduction systems that regulate their development and support successful infection of the ***,we characterized three components of the cell wall integrity-controlling pathway,namely protein kinase C(Ao PKC),SLT2-MAPK(Ao SLT2),and SWI6(Ao SWI6),in a representative nematode-trapping fungus Arthrobotrys oligospora,using gene disruption and multi-omics *** phenotypic traits(such as mycelia development,conidiation,stress response,and trap morphogenesis) and metabolic profiles of ΔAopkc and ΔAoswi6 mutants were similar but differed from those of the ΔAoslt2 *** analysis indicated that the genes differentially expressed in the absence of Aoswi6 were involved in DNA replication,repair,and recombination during trap ***,the yeast two-hybrid assay showed that Ao PKC interacted with Ao SWI6,suggesting that in ***,PKC can directly regulate SWI6,bypassing the SLT2signaling ***,our findings deepen our understanding of the regulatory mechanism of asexual development and lifestyle switching in nematode-trapping fungi.

关键词： Arthrobotrys oligospora conidiation stress response trap formation differential expression analysis metabolic profile pathogenicity

Food Safety Risks and Contributing Factors of Cronobacter spp.

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Engineering 2022年第5期8卷 128-138页

作者： Na Ling Xiuting Jiang Stephen Forsythe Danfeng Zhang Yizhong Shen Yu Ding Juan Wang Jumei Zhang Qingping Wu Yingwang Ye School of Food and Biological Engineering Hefei University of TechnologyHefei 230009China Guangdong Provincial Key laboratory of microbial Culture Collection and Application State Key Laboratory of Applied Microbiology Southern ChinaGuangdong Institute of MicrobiologyGuangdong Academy of SciencesGuangzhou 510070China *** Keyworth NG125GYUK

Cronobacter species are a group of Gram-negative opportunistic pathogens,which cause meningitis,sep-ticemia,and necrotizing enterocolitis in neonates and infants,with neurological sequelae in severe *** in Cronobacter has increased significantly in recent years due to its high virulence in *** this review,we summarize the current understanding of the prevalence of Cronobacter species in several important food *** discuss the response mechanisms enabling persistence in adverse growth con-ditions,as well as its *** emphasize the food safety concerns caused by Cronobacter and subsequent control methods and clinical treatments.

关键词： Cronobacter Food contamination Pathogenesis Virulence factors Stress response

Innovation for ascertaining genomic islands in PAO1 and PA14 of Pseudomonas aeruginosa

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Chinese Science Bulletin 2009年第21期54卷 3991-3999页

作者： SONG Lei ZHANG XueHong Key laboratory of microbial Metabolism Ministry of Education School of Life Sciences and Biotechnology Shanghai Jiao Tong University Shanghai 200240 China College of Life and Environment Science Shanghai Normal University Shanghai 200234 China

Based on three distinct traits of genomic islands, a novel approach was developed to search for and determine genomic islands in special strains. Two genomic islands in Pseudomonas aeruginosa PAO1 and 7 genomic islands in Pseudomonas aeruginosa PA14 were defined with this method. Among the 9 genomic islands, 4 islands had been characterized before, while the other 5 islands were initially determined. The insert sites of 6 genomic islands are tRNA sequences, direct repeats of PA14GI-3 are relative to tRNALeu, and direct repeats of PA14GI-2 are at the 3′ end of bifunctional GMP synthase/ glutamine amidotransferase. Only direct repeats of PA14GI-4 are not clear. Among the 5 newly-found genomic islands, it was supposed that PA14GI-2 is a genomic island related to Hg2+ uptake, PA14GI-3 is a secretory activity genomic island, PA14GI-6 is a pathogenicity island, and functions of PA14GI-1 and PA14GI-5 are not clear. Finally, the tyrosine type integrases in PAO1GI-1, PA14GI-5 and PA14GI-7 were analyzed, and their binding and restriction sites were predicted.

关键词：基因组铜绿假单胞菌岛屿创新 PA 绿脓杆菌 tRNA 谷氨酰胺

Resolving the Colletotrichum siamense species complex using ApMat marker

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Fungal Diversity 2015年第2期71卷 247-264页

作者： Gunjan Sharma Anil Kumar Pinnaka Belle Damodara Shenoy microbial Type Culture Collection and Gene Bank CSIR-Institute of Microbial TechnologySector 39-AChandigarh 160036India Biological Oceanography Division CSIR-National Institute of OceanographyDona PaulaGoa 403004India

Colletotrichum gloeosporioides sensu lato has been associated with anthracnose in diverse commercial *** is now established that *** sensu lato comprises 33 phylogenetic species and *** sensu stricto is not a common pathogen of tropical *** this study,we investigated the phylogenetic relationships of 85 Colletotrichum isolates associated with select tropical fruits and flowering plants from *** the ApMat marker analysis,the 85 isolates clustered with 7 known Colletotrichum species(***,***,***,***,***,***,Glomerella cingulata ***)and six novel *** of the novel lineages is described and illustrated in this paper as Colletotrichum communis ***.,while new-host pathogen associations for ***,***,*** and *** are reported from *** of the 85 isolates analysed in this paper,73 isolates clustered within the *** species complex,indicating that *** species complex,not *** sensu stricto,is common on tropical *** comparison with act,cal,gapdh,ITS and tub2 gene markers,we recommend the use of the ApMat marker for accurate identification of cryptic species within the *** species *** believe that the ApMat marker,in combination with one or two similar‘phylogenetically superior’gene markers,is a better candidate for specieslevel classification of fungi that were traditionally identified as‘Colletotrichum gloeosporioides’.

关键词： Apn2/MAT IGS Ascomycota pathogenicity testing Phylogeny Tropical crops

Camouflaging attenuated Salmonella by cryo-shocked macrophages for tumor-targeted therapy

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Signal Transduction and Targeted Therapy 2024年第2期9卷 709-723页

作者： Leyang Wu Zengzheng Du Lin Li Liyuan Qiao Shuhui Zhang Xingpeng Yin Xiaoyao Chang Chenyang Li Zichun Hua The State Key laboratory of Pharmaceutical Biotechnology and Department of Neurology of Nanjing Drum Tower Hospital School of Life Sciences and The Affiliated Hospital of Nanjing University Medical SchoolNanjing UniversityNanjing 21008 JiangsuChina Nanjing Generecom Biotechnology Co. LtdNanjing 210023China Changzhou High-Tech Research Institute of Nanjing University and Jiangsu TargetPharma Laboratories Inc Changzhou 213164 JiangsuChina School of Biopharmacy China Pharmaceutical UniversityNanjing 210023 JiangsuChina

Live bacteria-mediated antitumor therapies mark a pivotal point in cancer ***,the difficulty in reconciling the safety and efficacy of bacterial therapies has limited their *** bacterial tumor-targeted delivery while maintaining biosafety is a critical hurdle for the clinical translation of live microbial therapy for ***,we developed“dead”yet“functional”Salmonella-loaded macrophages using liquid nitrogen cold shock of an attenuated Salmonella typhimurium VNP20009-contained macrophage cell *** obtained“dead”macrophages achieve an average loading of approximately 257 live bacteria per 100 *** engineered cells maintain an intact cellular structure but lose their original pathogenicity,while intracellular bacteria retain their original biological activity and are delay freed,followed by ***“Trojan horse”-like bacterial camouflage strategy avoids bacterial immunogenicity-induced neutrophil recruitment and activation in peripheral blood,reduces the clearance of bacteria by neutrophils and enhances bacterial tumor enrichment efficiently after systemic ***,this strategy also strongly activated the tumor microenvironment,including increasing antitumor effector cells(including M1-like macrophages and CD8+Teffs)and decreasing protumor effector cells(including M2-like macrophages and CD4+Tregs),and ultimately improved antitumor efficacy in a subcutaneous H22 tumor-bearing mouse *** cryo-shocked macrophage-mediated bacterial delivery strategy holds promise for expanding the therapeutic applications of living bacteria for cancer.

关键词： shock holds maintain

Rapid Identification of Legionella pathogenicity by Surface-Enhanced Raman Spectroscopy

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Biomedical and Environmental Sciences 2015年第6期28卷 437-444页

作者： LI Jing QIN Tian JIA Xiao Xiao DENG Ai Hua ZHANG Xu FAN Wen Hui HUO Shuai Dong WEN Ting Yi LIU Wen Jun CAS Key laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of Sciences National Institute for Communicable Disease Control and Prevention and State Key laboratory for Infectious Disease Prevention and Control Chinese Centre for Disease Control and Prevention Collaborative Innovation Centre for Diagnosis and Treatment of Infectious Diseases University of Chinese Academy of Sciences CAS Key laboratory of microbial Physiological and Metabolic Engineering Institute of MicrobiologyChinese Academy of Sciences National Center for Nanoscience and Technology Chinese Academy of Sciences

Objective To establish Surface-enhanced Raman Spectroscopy（SERS） can be used as a rapid and reliable method to distinguish virulent strain and mild strain of L. pneumophila. Methods We isolated and characterized of bacterial strains from ATCC and water samples strains, while we analyzed data from SERS technology using gold nanoparticles as a base and cell infections were employed to rapidly detect L. pneumophila strains. Origin 8.0 was used to collect Raman spectra, smooth and homogenize data, and to contrast spectra. Principal component analysis（PCA） was conducted to discriminate differences between groups using the multivariate analysis package Py Chem 3.0.5. Results Our results indicated that the peaks of high virulence strains reached ≥4000. This criterion was verified by subsequent cell experiments. In addition, we also conducted SERS rapid identification on the virulence of several collected clinical strains and obtained accurate results. Conclusion The present study indicates that the established SERS protocol can be used as a rapid and reliable method to distinguish virulent and mildly virulent strains of L. pneumophila, which can be further used in clinical samples.

关键词： Legionella pneumophila Surface-enhanced Raman Spectroscopy pathogenicity Gold nanoparticles