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Insect Science 2024年第1期31卷 211-224页

作者： Yulong Wang Zhen Liu Xuebing Yin Shihong Liu Kai Wang Rongjie Wan Haoran Chen Xinyang Li Bo Huang Anhui Provincial Key laboratory of microbial Pest Control Anhui Agricultural UniversityHefeiChina

Entomopathogenic fungi are protected by a cell wall with dynamic structure for adapting to various environmental conditions.β-1,3-Glucan recognition proteins activate the innate immune system of insects by recognizing surface molecules of ***,the associations between pathogenicity and the different components of entomopathogenic fungal cell walls remain *** Beauveria bassiana strains were selected that have significantly differing virulence against Bombyx *** molecular mechanisms underlying the immune response in *** were investigated using RNA sequencing,which revealed differences in the immune response to different *** strains at 12 h *** assays revealed thatβ-1,3-glucan content had an opposite trend to that of fungal virulence.β-1,3-Glucan injection upregulated BmβGRP4 expression and significantly reduced the virulence of the high-virulence strain but not that of the medium-virulence or low-virulence ***βGRP4 silencing in *** with RNA interference resulted in the opposite virulence pattern,indicating that the virulence of *** was affected by the cell walls’content ofβ-1,3-glucan,which could be recognized by Bmβ***,interference with the gene CnA(calcineurin catalytic A subunit)involved inβ-1,3-glucan synthesis eliminated differences in virulence between *** *** results indicate that strains of a single species of pathogenic fungi that have differing cell wall components are recognized differently by the innate immune system of ***.

关键词： ombyx mori BmβGRP4 innate immunity insect fungal pathogen virulence

Mechanistic research:Selenium regulates virulence factors,reducing adhesion ability and inflammatory damage of Helicobacter pylori

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World Journal of Gastroenterology 2024年第1期30卷 91-107页

作者： Chun Qin Gan-Rong Huang Ai-Xing Guan Wen-Ting Zhou Hao Chen Pei-Pei Luo Xian-Ke Luo Yan-Qiang Huang Zan-Song Huang Guangxi Technology Innovation Cooperation Base of Prevention and Control Pathogenic Microbes with Drug Resistance Youjiang Medical University for NationalitiesBaise 533000Guangxi Zhuang Autonomous RegionChina Department of Digestive Diseases Affiliated Hospital of Youjiang Medical University for NationalitiesBaise 533000Guangxi Zhuang Autonomous RegionChina Key laboratory of the Prevention and Treatment of Drug Resistant microbial Infecting Youjiang Medical University for NationalitiesBaise 533000Guangxi Zhuang Autonomous RegionChina Department of Pathology Wannan Medical CollegeWuhu 241002Anhui ProvinceChina Department of Gastroenterology Wujin People’s Hospital affiliated to Jiangsu UniversityChangzhou 213004Jiangsu ProvinceChina Department of Gastroenterology Guangzhou Liwan District People's HospitalGuangzhou 510370Guangdong ProvinceChina

BACKGROUND The pathogenicity of Helicobacter pylori is dependent on factors including the environment and the *** selenium is closely related to pathogenicity as an environmental factor,the specific correlation between them remains *** To investigate how selenium acts on virulence factors and reduces their *** *** strains were induced by sodium *** expression of cytotoxin-associated protein A(CagA)and vacuolating cytotoxin gene A(VacA)was determined by quantitative PCR and Western *** was used to analyze CagA,CagM,CagE,Cag1,Cag3,and CagT.C57BL/6A mice were infected with the attenuated strains subjected to sodium selenite induction,and *** colonization,inflammatory reactions,and the cell adhesion ability of *** were *** CagA and VacA expression was upregulated at first and then downregulated in the *** strains after sodium selenite *** expression was significantly and steadily downregulated after the 5th cycle(10 d).Transcriptome analysis revealed that sodium selenite altered the levels affect *** virulence factors such as CagA,CagM,CagE,Cag1,Cag3,and *** these factors,CagM and CagE expression was continuously downregulated and further downregulated after 2 h of induction with sodium ***,CagT expression was upregulated before the 3rd cycle(6 d)and significantly downregulated after the 5th ***1 and Cag3 expression was upregulated and downregulated,respectively,but no significant change was observed by the 5th cycle.C57BL/6A mice were infected with the attenuated strains subjected to sodium selenite *** extent of *** colonization in the stomach increased;however,sodium selenite also induced a mild inflammatory reaction in the gastric mucosa of ***-infected mice,and the cell adhesion ability of *** was significantly *** These results demonstrate that *** displayed virulence attenuation after

关键词： Helicobacter pylori Sodium selenite Virulence factors Adherence Inflammation

Investigation of DNA Sequences Related to Latency-Associated Transcripts in the Genome of Canine Herpesvirus Type 1 (CHV-1) by Means of Bioinformatics Tools

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Open Journal of Veterinary Medicine 2019年第10期9卷 147-160页

作者： Ortiz M. A. Herná ndez Verde C. Cuenca Lara E. G. Valdivia Anda G. Valdivia laboratory of microbial pathogenicity Multidisciplinary Research Unit Faculty of Higher Education Cuautitlan National Autonomous University of Mexico Cuautitlan Izcalli Mexico

A characteristic common to herpesviruses is the ability to establish a latent infection in the hosts, a transcriptionally active region has detected during latency as well as a set of RNA that are known as Latency Associated Transcripts (LATs), their functions have been clarified in recent work. The present work was carried using different bioinformatics method in order to determine if Herpesvirus Canine 1 (CHV-1) has a region associated with latency. Our result was the selection of nine sequences candidate of micro RNA (miRNA) (MIREval 2.0 software), and 26 miRNA (miRNAFold v.1.0 software), of them, were selected 14 with real precursors of miRNA, two were found between the RL2 and RS1 genes, one in the RL2 gene and 11 in the RS1 gene. The results showed that the similarities of these regions are very low among the herpesviruses analyzed, so it was not possible to deduce the presence of the LAT gene in canine herpesvirus type 1 with bioinformatics. On the other hand, the comparison showed that the miRNA predicted: chv1-mir-mirnafold-8 has similarity with the ebv-mir-BART7-3p of Epstein-Barr Virus (EBV), in this way, the microRNAs predicted by means of bioinformatic programs met the theoretical requirements of these molecules, however at not having a degree of preservation in other herpesviruses, the expression by CHV-1 in latency cannot be confirmed and it is necessary to identify through experimental tests.

关键词： Latency Canine Herpesvirus Latency-Associated Transcripts

Nematode-induced trap formation regulated by the histone H3K4 methyltransferase AoSET1 in the nematode-trapping fungus Arthrobotrys oligospora

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Science China(Life Sciences) 2023年第11期66卷 2663-2679页

作者： Qiao Miao Zhengqi Wang Ziyu Yin Xiaoying Liu Ran Li Ke-Qin Zhang Juan Li State Key laboratory for Conservation and Utilization of Bio-Resources Key Laboratory for Microbial Resources of the Ministry of EducationSchool of Life SciencesYunnan UniversityKunming 650091China

The methylation of lysine 4 of histone H3(H3K4),catalyzed by the histone methyltransferase KMT2/SET1,has been functionally identified in many pathogenic fungi but remains unexplored in nematode-trapping fungi(NTFs).Here,we report a regulatory mechanism of an H3K4-specific SET1 orthologue,AoSET1,in the typical nematode-trapping fungus Arthrobotrys *** the fungus is induced by the nematode,the expression of AoSET1 is *** of AoSet1 led to the abolishment of ***,the yield of traps and conidia ofΔAoSet1 was significantly lower than that of the WT strain,and the growth rate and pathogenicity were also ***,H3K4 trimethylation was enriched mainly in the promoter of two bZip transcription factor genes(AobZip129 and AobZip350)and ultimately up-regulated the expression level of these two transcription factor *** theΔAoSet1 and AoH3K4A strains,the H3^(K4)me modification level was significantly decreased at the promoter of transcription factor genes AobZip129 and *** results suggest that AoSET1-mediated H3KEme serves as an epigenetic marker of the promoter region of the targeted transcription factor ***,we found that AobZip129 negatively regulates the formation of adhesive networks and the pathogenicity of downstream AoPABP1 and *** findings confirm that the epigenetic regulatory mechanism plays a pivotal role in regulating trap formation and pathogenesis in NTFs,and provide novel insights into the mechanisms of interaction between NTFs and nematodes.

关键词： Arthrobotrys oligospora trap formation epigenetic regulatory histone modification pathogenicity

Species-level Microbiota of Biting Midges and Ticks from Poyang Lake

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Biomedical and Environmental Sciences 2024年第3期37卷 266-277,I0001-I0003页

作者： GONG Jian WANG Fei Fei LIU Yang Qing PU Ji DONG Ling Zhi ZHANG Si Hui HUANG Zhen Zhou HUANG Yu Yuan LI Ya Ben YANG Cai Xin TAO Yuan Meihui ZHAO Li Jun JIN Dong LIU Li Yun YANG Jing LU Shan Department of Epidemiology School of Public HealthShanxi Medical UniversityTaiyuan 030001ShanxiChina National Key laboratory of Intelligent Tracking and Forecasting for Infectious Diseases National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Jiangxi Provincial Key laboratory of Animal-Origin and Vector-Borne Diseases Nanchang Center for Disease Control and PreventionNanchang 330038JiangxiChina Research Units of Discovery of Unknown Bacteria and Function Chinese Academy of Medical SciencesBeijing 100730China

Objective The purpose of this study was to investigate the bacterial communities of biting midges and ticks collected from three sites in the Poyang Lake area,namely,Qunlu Practice Base,Peach Blossom Garden,and Huangtong Animal Husbandry,and whether vectors carry any bacterial pathogens that may cause diseases to humans,to provide scientific basis for prospective pathogen discovery and disease prevention and *** Using a metataxonomics approach in concert with full-length 16S rRNA gene sequencing and operational phylogenetic unit(OPU)analysis,we characterized the species-level microbial community structure of two important vector species,biting midges and ticks,including 33 arthropod samples comprising 3,885 individuals,collected around Poyang *** A total of 662 OPUs were classified in biting midges,including 195 known species and 373 potentially new species,and 618 OPUs were classified in ticks,including 217 known species and 326 potentially new ***,OPUs with potentially pathogenicity were detected in both arthropod vectors,with 66 known species of biting midges reported to carry potential pathogens,including Asaia lannensis and Rickettsia bellii,compared to 50 in ticks,such as Acinetobacter lwoffii and Staphylococcus *** found that Proteobacteria was the most dominant group in both midges and ***,the outcomes demonstrated that the microbiota of midges and ticks tend to be governed by a few highly abundant *** sp7 was predominant in biting midges,while Coxiella sp1 was enriched in ***,Coxiella spp.,which may be essential for the survival of Haemaphysalis longicornis Neumann,were detected in all tick *** identification of dominant species and pathogens of biting midges and ticks in this study serves to broaden our knowledge associated to microbes of arthropod *** Biting midges and ticks carry large numbers of known and potentially novel bacteria,and carry a wide

关键词： Biting midge Tick Microbiota Pantoea Coxiella

Action modes of transcription activator-like effectors(TALEs) of Xanthomonas in plants

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Journal of Integrative Agriculture 2017年第12期16卷 2736-2745页

作者： XU Zheng-yin ZOU Li-fang MA Wen-xiu CAI Lu-lu YANG Yang-yang CHEN Gong-you School of Agriculture and Biology/State Key laboratory of microbial Metabolism Shanghai Jiao Tong University Shanghai200240 P.R.China

Plant-pathogenic Xanthomonas infects a wide variety of host plants and causes many devastating diseases on crops. Transcription activator-like effectors（TALEs） are delivered by a type III secretion system（T3 SS） of Xanthomonas into plant nuclei to directly bind specific DNA sequences（TAL effector-binding elements, EBEs） on either strand of host target genes with an unique modular DNA-binding domain and to bidirectionally drive host gene transcription. The target genes in plants consist of host susceptibility（S） genes promoting disease（ETS） and resistance（R） genes triggering defense（ETI）. Here we generally summarized the discovery of TALEs in Xanthomonas species, their functions in bacterial pathogenicity in plants and their target genes in different host plants, and then focused on the newly revealed modes of protein action in triggering or suppressing plant defense.

关键词： Xanthomonas TALE iTALE ETS ETI suppressor

AoATG5 plays pleiotropic roles in vegetative growth,cell nucleus development,conidiation,and virulence in the nematode-trapping fungus Arthrobotrys oligospora

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Science China(Life Sciences) 2022年第2期65卷 412-425页

作者： Duanxu Zhou Yingmei Zhu Na Bai Le Yang Meihua Xie Jiangliu Yang Meichen Zhu Ke-Qin Zhang Jinkui Yang State Key laboratory for Conservation and Utilization of Bio-Resources and Key laboratory for microbial Resources of the Ministry of Education Yunnan UniversityKunming 650091China School of Life Sciences Yunnan UniversityKunming 650091China

Autophagy is an evolutionarily conserved process in eukaryotes,which is regulated by autophagy-related genes(ATGs).Arthrobotrys oligospora is a representative species of nematode-trapping(NT)fungi that can produce special traps for nematode *** elucidate the biological roles of autophagy in NT fungi,we characterized an orthologous Atg protein,AoAtg5,in *** found that AoATG5 deletion causes a significant reduction in vegetative growth and conidiation,and that the transcript levels of several sporulation-related genes were significantly downregulated during sporulation *** addition,the cell nuclei were significantly reduced in theΔAoATG5 mutant,and the transcripts of several genes involved in DNA bio-synthesis,repair,and ligation were significantly ***ΔAoATG5 mutants,the autophagic process was significantly impaired,and trap formation and nematocidal activity were significantly *** transcriptome analysis results showed that AoAtg5 is involved in the regulation of multiple cellular processes,such as autophagy,nitrogen metabolism,DNA biosynthesis and repair,and vesicular *** summary,our results suggest that AoAtg5 is essential for autophagy and significantly contributes to vegetative growth,cell nucleus development,sporulation,trap formation,and pathogenicity in ***,thus providing a basis for future studies focusing on related mechanisms of autophagy in NT fungi.

关键词： Arthrobotrys oligospora autophagy-related gene AoATG5 phenotypic traits transcriptome analysis sporulation cell nucleus development

MAPKs and acetyl-CoA are associated with Curvularia lunata pathogenicity and toxin production in maize

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Journal of Integrative Agriculture 2018年第1期17卷 139-148页

作者： NI Xuan GAO Jin-xin YU Chuan-jin WANG Meng SUN Jia-nan LI Ya-qian CHEN Jie School of Agriculture and Biology Shanghai Jiao Tong University/State Key Laboratory of Microbial Metabolism Shanghai JiaoTong UniversityKey Laboratory of Urban Agriculture (South) Ministry of Agriculture Shanghai 200240 P.R.China

Mitogen-activated protein kinase （MAPK） cascades play an important role in extracellular signal transduction and are involved in the pathogenicity of fungal pathogens to host plants. In Curvularia lunata, the roles of two MAPK genes, Clkl and CIm 1, have already been studied. Clkl is involved in conidia formation and pathogenicity, and Clmf is closely related to pathogen cell wall formation and pathogenicity to maize leaves. In this study, a third C. lunata MAPK gene, Clhl, which is homologous to hog1, was successfully cloned. We found that a Clhl deletion mutant had lower intracellular glycerol accumulation than the wild-type stain and was unable to grow normally under osmotic stress conditions. Furthermore, the deletion mutants of three C. lunata MAPK genes （Clkl, Clml and Clhl） had lower levels of acetyI-CoA, which is an important intermediate product in the synthesis of melanin and furan toxin, and down-regulated expression of pathogenicity-associated genes. Furthermore, pathogenicity and the ability to produce toxin were restored after adding acetyI-CoA to the culture medium, suggesting that acetyI-CoA is closely involved in the pathogen MAPK signaling pathway.

关键词： Curvularia lunata MAP kinase acetyI-CoA pathology

Sod gene of Curvularia lunata is associated with the virulence in maize leaf

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Journal of Integrative Agriculture 2017年第4期16卷 874-883页

作者： GAO Shi-gang NI Xuan LI Ying-ying FU Ke-he YU Chuan-jin GAO Jin-xin WANG Meng LI Ya-qian CHEN Jie School of Agriculture and Biology Shanghai Jiao Tong University/State Key Laboratory of Microbial MetabolismShanghai Jiao Tong University/Key Laboratory of Urban Agriculture(South)Ministry of Agriculture School of Biology Nanchang Normal University

Curvularia leaf spot, caused mainly by Curvularia lunata, is a widespread plant disease in China. In the recent years, di- rectional host selection by the pathogen, which likely results in the virulence differentiation in pathogens, is widely reported. Among the hallmarks potentially associated to pathogen variation in virulence, superoxide dismutase gene Sod has been found to be closely related to the enhancement of virulence. In the present study, the full-length of Sod was obtained via Blastn alignment against GenBank and the whole genome of C. lunata. In order to understand the role of Sod in the vir- ulence variation in C. lunata, targeted gene disruption was performed to construct Sod mutants. The cell wall degrading enzyme （CWDE） activities and toxin production of ASod were not distinctly different from wild-type strain CX-3 and its complon. However, at an early stage of infection, 3Sod virulence appeared to be lower than CX-3 and the complon, while at a later stage, its virulence gradually returned to the level of CX-3 and the complon. Furthermore, the melanin production of ASod was significantly reduced compared to CX-3 and the complon, suggesting that Sod gene influences the virulence by regulating melanin production at an early stage of infection but is not essential for pathogenicity. However, the disruption of Sod did not significantly affect the transcriptional expression of the melanin biosynthesis-associated genes, bml and scd. Therefore, we infer that Sod in C. lunata are involved, to some extent, with the virulence in maize leaf, but still needs further studies to have a clear understanding of its mechanism.

关键词： Curvularia lunata Sod gene function infection

Triggering the biocontrol of Botrytis cinerea by Trichoderma harzianum through inhibition of pathogenicity and virulence related proteins

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Frontiers of Agricultural Science and Engineering 2018年第2期5卷 271-279页

作者： Kandasamy SARAVANAKUMAR Zhixiang LU Hai XIA Meng WANG Jianan SUN Shaoqing WANG Qiang-qiang WANG Yaqian LI Jie CHEN School of Agriculture and Biology/State Key laboratory of microbial Metabolism/Key laboratory of Urban Agriculture (South) of the Ministry of Agriculture Shanghai Jiao Tong University

This study reports a strain of Trichoderma harzianum CCTCC-SBW0162 with potential to enhance biocontrol activity against gray mold pathogen, Botrytis cinerea, and with a pivotal role in tomato(Solanum esculentum) plant growth enhancement. A total of 254 Trichoderma isolates were screened by in vitro antagonistic assay. Of these, 10 were selected for greenhouse experiments based on their greater inhibition of B. *** in vitro antagonistic assay and greenhouse experiments indicated that T. harzianum CCTCC-SBW0162 gave the highest inhibition rate(90.6%) and disease reduction(80.7%). Also, to study the possible mechanism associated with antifungal activity of CCTCC-SBW0162 against B. cinerea, molecular docking was used to assess the interactions between CCTCC-SBW0162-derived metabolites, and pathogencity and virulence related proteins of B. cinerea. The molecular docking results indicated that the combination of harzianopyridone,harzianolide and anthraquinone C derived from CCTCCSBW0162 could synergistically improve antifungal activity against B. cinerea through the inhibition/modification of pathogenicity and virulence related ***, this computerized modeling work emphasized the need for further study in the laboratory to confirm the effect T. harzianum-derived metabolites against the proteins of B. cinerea and their interactions.

关键词： anthraquinone Botrytis cinerea harzianolide harzianopyridone molecular docking Trichoderma harzianum