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Zinc lactate alleviates oxidative stress by modulating crosstalk between constitutive androstane receptor signaling pathway and gut microbiota profile in weaned piglets

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Animal Nutrition 2024年第1期16卷 23-33页

作者： Wenjie Tang Xuan Xiang Houfu Wang Wentao Zhou Liuqin He Yulong Yin Tiejun Li College of Animal Science and Technology Hunan Agricultural University Sichuan Academy of Animal Sciences Animal Breeding and Genetics Key Laboratory of Sichuan Province Livestock and Poultry Biological Products Key laboratory of Sichuan Province Sichuan Animtech Feed Co. Ltd Hunan Provincial Key laboratory of Animal Nutritional Physiology and Metabolic Process CAS Key Laboratory of Agro-Ecological Processes in Subtropical Region National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production Institute of Subtropical Agriculture Chinese Academy of Sciences College of Advanced Agricultural Science University of Chinese Academy of Sciences Hunan Provincial Key laboratory of Animal Intestinal Function and Regulation Laboratory of Animal Nutrition and Human Health College of Life Sciences Hunan Normal University

This study aimed to determine the regulatory mechanism of dietary zinc lactate (ZL) supplementation on intestinal oxidative stress damage in a paraquat (PQ)-induced piglet model. Twenty-eight piglets (mean body weight 9.51±0.23 kg) weaned at 28 d of age were randomly divided into control, ZL, PQ, and ZL+PQ groups (n=7 in each group). The ZL-supplemented diet had little effect on growth performance under normal physiological conditions. However, under PQ challenge, ZL supplementation significantly improved average daily gain (Ptransport and absorption in PQ-induced piglets, which reduced intestinal permeability. ZL supplementation also enhanced the expression of antioxidant and antiinflammatory factor-related genes and decreased inflammatory cytokine expression and secretion in PQinduced piglets. Furthermore, ZL treatment significantly inhibited the activation of constitutive androstane receptor (CAR) signaling (Psignaling and gut microbiota.

关键词： Zinc lactate Intestinal barrier Oxidative stress Inflammation Constitutive androstane receptor Gut microbiota

Structural and biochemical basis of FLS2-mediated signal activation and transduction in rice

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Plant Communications 2024年第3期5卷 216-231页

作者： Qiaoqiao Zhao Jinlin Bao Huailong Li Wei Hu Yanqiong Kong Yifeng Zhong Qiang Fu Guolyu Xu Fenmei Liu Xi Jiao Jian Jin Zhenhua Ming State Key laboratory for Conservation and Utilization of Subtropical Agro-bioresources College of Life Science and TechnologyGuangxi Key Laboratory for Sugarcane BiologyGuangxi UniversityNanning 530004P.R.China

The receptor-like kinase FLAGELLIN-SENSITIVE 2(FLS2)functions as a bacterialflagellin receptor local-ized on the cell membrane of *** Arabidopsis,the co-receptor BRI1-ASSOCIATED RECEPTOR KI-NASE 1(BAK1)cooperates with FLS2 to detect theflagellin epitopeflg22,resulting in formation of a signaling complex that triggers plant defense ***,the co-receptor responsible for recog-nizing and signaling theflg22 epitope in rice remains to be determined,and the precise structural mecha-nism underlying FLS2-mediated signal activation and transduction has not been *** study pre-sents the structural characterization of a kinase-dead mutant of the intracellular kinase domain of OsFLS2(OsFLS2-KDD1013A)in complex with ATP or ADP,resolved at resolutions of 1.98 A˚and 2.09 A˚,*** analysis revealed that OsFLS2 can adopt an active conformation in the absence of phosphorylation,although it exhibits only weak basal catalytic activity for ***-quent investigations demonstrated that OsSERK2 effectively phosphorylates OsFLS2,which reciprocally phosphorylates OsSERK2,leading to complete activation of OsSERK2 and rapid phosphorylation of the downstream substrate receptor-like cytoplasmic kinases OsRLCK176 and *** mass spectrometry experiments,we successfully identied critical autophosphorylation sites on OsSERK2,as well as sites transphosphorylated by ***,we demonstrated the interaction between OsSERK2 and OsFLS2,which is enhanced in the presence *** evidence suggests that OsRLCK176 and OsRLCK185 may function downstream of the OsFLS2-mediated signaling *** study reveals the molecular mechanism by which OsFLS2 mediates signal transduction pathways in rice and provides a valuable example for understanding RLK-mediated signaling pathways in plants.

关键词： FLS2 active conformation kinase activation transphosphorylation signal transduction

Monitoring of Cell Membrane Microenvironment Based on DNA Nanodevices

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Chemical Research in Chinese Universities 2024年第2期40卷 255-267页

作者： NAN Hexin CAI Ming KUANG Shi NIE Zhou State Key laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringHunan Provincial Key Laboratory of Biomacromolecular Chemical BiologyHunan UniversityChangsha410082P.R.China

The cell membrane is a critical barrier for cellular homeostasis,integral to signaling and intercellular communication,and vital for understanding cellular functions and disease *** its microenvironment is crucial for uncovering the molecular basis of physiological and pathological processes associated with the cell membrane,driving the development of bioanalytical toolkits capable of dynamically monitoring the cell surface *** the continuous advancement of functional nucleic acids and dynamic DNA nanotechnology,DNA nanodevices with controllable nanosized geometry,specific molecular recognition,and selective membrane-localization properties offer a versatile platform for probing the cell membrane *** this review,we summarize the current biosensing and membrane-anchoring mechanisms of DNA nanodevices and highlight their use in studying key cell membrane events,including membrane lipid dynamics,transmembrane transport,receptor dimerization,and signal ***,we discuss the challenges and potential future applications of DNA nanodevices in advancing cell membrane biology research and biomedical applications.

关键词： DNA nanodevice Cell membrane Cellular micro-environment Monitoring

The miR159a-DUO1 module regulates pollen development by modulating auxin biosynthesis and starch metabolism in citrus

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Journal of Integrative Plant Biology 2024年第7期66卷 1351-1369页

作者： Yanhui Xu Wenxiu Tian Minqiang Yin Zhenmei Cai Li Zhang Deyi Yuan Hualin Yi Juxun Wu National Key laboratory for Germplasm Innovation&Utilization of Horticultural Crops Huazhong Agricultural UniversityWuhan 430070China Key laboratory of Cultivation and Protection for Non‐Wood Forest Trees of the Ministry of Education and Key laboratory of Non‐Wood Forest Products of the Forestry Ministry Central South University of Forestry and TechnologyChangsha 410004China

Achieving seedlessness in citrus varieties is one of the important objectives of citrus *** sterility associated with abnormal pollen development is an important factor in ***,our understanding of the regulatory mechanism underlying the seedlessness phenotype in citrus is still ***,we determined that the miR159a-DUO1 module played an important role in regulating pollen development in citrus,which further indirectly modulated seed development and fruit *** the overexpression of csi-miR159a and the knocking out of DUO1 in Hong Kong kumquat(Fortunella hindsii)resulted in small and seedless fruit ***,pollen was severely aborted in both transgenic lines,with arrested pollen mitotic I and abnormal pollen starch *** additional cross-pollination experiments,DUO1 was proven to be the key target gene for miR159a to regulate male sterility in *** on DNA affinity purification sequencing(DAP-seq),RNA-seq,and verified interaction assays,YUC2/YUC6,SS4 and STP8 were identified as downstream target genes of DUO1,those were all positively regulated by *** transgenic *** lines,the miR159a-DUO1 module down-regulated the expression of YUC2/YUC6,which decreased indoleacetic acid(IAA)levels and modulated auxin signaling to repress pollen mitotic *** miR159a-DUO1 module reduced the expression of the starch synthesis gene SS4 and sugar transport gene STP8 to disrupt starch metabolism in ***,this work reveals a new mechanism by which the miR159a-DUO1 module regulates pollen development and elucidates the molecular regulatory network underlying male sterility in citrus.

关键词： auxin citrus male sterility miRNA pollen development seedlessness starch metabolism

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The RhoA nuclear localization changes in replicative senescence:New evidence from in vitro human mesenchymal stem cells studies

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BIOCELL 2022年第9期46卷 2053-2058页

作者： DANILA BOBKOV ANASTASIA POLYANSKAYA ANASTASIA MUSORINA GALINA POLJANSKAYA Collection of Vertebrate Cell Cultures Institute of CytologyRussian Academy of SciencesSt.Petersburg194064Russia laboratory of intracellular signaling and transport Smorodintsev Research Institute of InfluenzaSt.Petersburg197376Russia Research laboratory of Translational Oncology Almazov National Research CentreSt.Petersburg197341Russia

All non-immortalized mesenchymal stem cells have a limited proliferative potential,that is,replicative senescence(RS)is an integral characteristic of the life of all mesenchymal stem cells(MSCs).It is known that one of the important signs of RS is a decrease of cell motility,and that violations of migration processes contribute to the deterioration of tissue ***,the characterization of the properties of the cell line associated with RS is a prerequisite for the effective use of MSCs in restorative *** of the key proteins regulating cell motility is the small GTPase *** main purpose of this work was to study the nuclear-cytoplasmic redistribution of the RhoA protein during RS in MSC lines recently obtained and characterized in our *** study found that a comparative analysis of the intracellular localization of RhoA in three cell lines(MSCWJ-1,FetMSC,DF2)showed a decrease in the nuclear localization of RhoA during RS.

关键词： Mesenchymal stem cells RhoA Actin cytoskeleton Replicative senescence

Examination of wnt signaling mediated melanin transport and shell color formation in Pacific oyster(Crassostrea gigas)

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Marine Life Science & Technology 2024年第3期6卷 488-501页

作者： Yue Min Qi Li Hong Yu Shaojun Du Key laboratory of Mariculture Ministry of Educationand College of FisheriesOcean University of ChinaQingdao 266003China laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China Institute of Marine and Environmental Technology Department of Biochemistry and Molecular BiologyUniversity of Maryland School of MedicineBaltimore 21240USA

Mollusca exhibit remarkable diversity in shell coloration,attributed to the presence of melanin,a widely distributed pigment with various essential roles,such as mechanical strengthening,antioxidation and ***,the regulatory network governing melanogenesis and melanin transport in molluscs remains poorly *** this study,we conducted a systematic analysis of melanin distribution and transport in the Pacific oyster,utilizing light microscopy and high-resolution transmission electron *** addition,we characterized CgWnt1 and CgWnt2b-a in Crassostrea gigas,and analyzed Wnt signaling in melanocyte *** analysis revealed that these genes were predominantly expressed in the mantle of black-shelled individuals,particularly in the outer fold of the ***,we employed RNA interference and inhibitors to specifically inhibit Wnt signaling in both in vivo and in *** results revealed impaired melanogenesis and diminished tyrosinase activity upon Wnt signaling *** findings suggest the crucial role of Wnt ligands and downstream factors in *** summary,our study provides valuable insights into the regulatory mechanism of shell pigmentation in *** demonstrating the promotion of melanogenesis through Wnt signaling modulation,we contribute to a better understanding of the complex processes underlying molluscan melanin production and shell coloration.

关键词： Melanogenesis Melanosome trafficking WIF-1

Synthesis and potential osteogenic applications of Wnt3a-loaded ZIF-8 nanoparticles

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Chinese Chemical Letters 2024年第3期35卷 399-405页

作者： Hengfei Wang Song Chen Zihan He Junyu Chen Zhou Zhu Qianbing Wan Jian Wang Xibo Pei State Key laboratory of Oral Diseases National Clinical Research Center for Oral DiseasesDepartment of ProsthodonticsWest China Hospital of StomatologySichuan UniversityChengdu 610041China Chongqing Key laboratory of Oral Diseases and Biomedical Sciences Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher EducationStomatological Hospital of Chongqing Medical UniversityChongqing 400016China

The Wnt signaling pathway plays a critical role in bone homeostasis,and the related protein therapy strategies have been reported to have great potential in osseointegration;however,they face formidable challenges such as complex external environments and unavoidable protein *** this work,we report a novel approach combining the synthesis of metal–organic frameworks(MOFs)and protein encapsulation in a one-pot process based on zeolitic imidazolate framework-8(ZIF-8)and Wnt3a protein,with improved biomechanical behavior and enhanced protein biological *** combination was designed to enhance the Wnt3a protein function through the improved chemical stability provided by the ZIF-8 ***,the zinc ions contained in the ZIF-8 crystals induced bone homeostasis,further favoring the *** results showed that the Wnt3a protein-loaded ZIF-8 crystals served as efficient drug delivery vehicles to promote osteogenesis,preventing protein *** particular,Wnt3a-loaded ZIF-8 nanoparticles(Wnt3a@ZIF-8 NPs)had higher efficacy on bone marrow mesenchymal stem cells(BMSCs)than ZIF-8 NPs or Wnt3a proteins,contributing to the osteogenesis through ZIF-8 crystals and intracellular Wnt3a proteins released from Wnt3a@ZIF-8 ***,polymerase chain reaction(PCR)analysis showed that the osteogenic pathways were ***,the present one-pot process can open up new avenues to develop signaling protein-delivery systems for applications in protein therapy strategies.

关键词： Metal–organic frameworks Nanoparticle Drug release Osteogenesis Bone defects

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A reference-grade genome of the xerophyte Ammopiptanthus mongolicus sheds light on its evolution history in legumes and droughttolerance mechanisms

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Plant Communications 2024年第7期5卷 157-177页

作者： Lei Feng Fei Teng Na Li Jia-Cheng Zhang Bian-Jiang Zhang Sau-Na Tsai Xiu-Le Yue Li-Fei Gu Guang-Hua Meng Tian-Quan Deng Suk-Wah Tong Chun-Ming Wang Yan Li Wei Shi Yong-Lun Zeng Yue-Ming Jiang Weichang Yu Sai-Ming Ngai Li-Zhe An Hon-Ming Lam Jun-Xian He School of Life Sciences and State Key laboratory of Agrobiotechnology The Chinese University of Hong KongSha TinNew TerritoriesHong KongChina Guangdong Provincial Key laboratory of Biotechnology for Plant Development School of Life SciencesSouth China Normal UniversityGuangzhou 510631China BGI-Shenzhen Tech Co. Ltd.Beishan Industrial ZoneYantian DistrictShenzhen 518083China School of Life Sciences and Ministry of Education Key laboratory of Cell Activities and Stress Adaptations Lanzhou UniversityLanzhou 730030China State Key laboratory of Subtropical Silviculture Zhejiang Agriculture and Forestry UniversityHangzhou 311300China State Key laboratory of Plant Diversity and Specialty Crops South China Botanical GardenChinese Academy of SciencesGuangzhou 510650China Guangdong Provincial Key laboratory of Applied Botany&Key laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement South China Botanical GardenChinese Academy of SciencesGuangzhou 510650China College of Life Sciences and Oceanography Shenzhen UniversityShenzhen 518060China State Key laboratory of Efficient Production of Forest Resources Beijing Forestry UniversityBeijing 100083China

Plants that grow in extreme environments represent unique sources of stress-resistance genes and *** mongolicus(Leguminosae)is a xerophytic evergreen broadleaf shrub native to semi-arid and desert regions;however,its drought-tolerance mechanisms remain poorly ***,we report the assembly of a reference-grade genome for ***,describe its evolutionary history within the legume family,and examine its drought-tolerance *** assembled genome is 843.07 Mb in length,with 98.7%of the sequences successfully anchored to the nine chromosomes of *** genome is predicted to contain 47611 protein-coding genes,and 70.71%of the genome is composed of repetitive sequences;these are dominated by transposable elements,particularly longterminal-repeat *** analyses revealed two whole-genome duplication(WGD)events at 130 and 58 million years ago(mya)that are shared by the genus Ammopiptanthus and other legumes,but no species-specific WGDs were found within this *** genome reconstruction revealed that the *** genome has undergone fewer rearrangements than other genomes in the legume family,confirming its status as a"relict plant".Transcriptomic analyses demonstrated that genes involved in cuticular wax biosynthesis and transport are highly expressed,both under normal conditions and in response to polyethylene glycol-induced *** induction of genes related to ethylene biosynthesis and signaling was also observed in leaves under dehydration stress,suggesting that enhanced ethylene response and formation of thick waxy cuticles are two major mechanisms of drought tolerance in *** expression of AmERF2,an ethylene response factor unique to ***,can markedly increase the drought tolerance of transgenic Arabidopsis thaliana plants,demonstrating the potential for application of *** genes in crop improvement.

关键词： Ammopiptanthus mongolicus genome sequencing genome evolution drought tolerance cuticular wax ethylene

Artemisia vulgaris Induces Tumor-Selective Ferroptosis and Necroptosis via Lysosomal Ca^(2+) signaling

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Chinese Journal of Integrative Medicine 2024年第6期30卷 525-533页

作者： Lucas dos Santos Zamarioli Michele Rosana Maia Santos Adolfo Garcia Erustes Vanessa Marques Meccatti Thais Cristine Pereira Soraya S Smaili Maria Cristina Marcucci Carlos Rocha Oliveira Gustavo JS Pereira Claudia Bincoletto Department of Pharmacology Paulista School of MedicineFederal University of São PauloSão PauloSP04044-020Brazil Research Group on Phytocomplexes and Cell signaling School of Health SciencesAnhembi Morumbi UniversitySão PauloSP03164-000Brazil Department of Biosciences and Oral Diagnosis Institute of Science and TechnologySão Paulo State University(ICT-UNESP)São PauloSP12231-280Brazil

Objective To evaluate the chemical composition and effects of Artemisia vulgaris(AV)hydroalcoholic extract(HEAV)on breast cancer cells(MCF-7 and SKBR-3),chronic myeloid leukemia(K562)and NIH/3T3 *** Phytochemical analysis of HEAV was done by high-performance liquid chromatography-mass(HPLC)*** and cell death studies were performed using trypan blue and Annexin/FITC-7AAD,***-1(Fer-1)and necrostatin-1(Nec-1)were used to assess the mode of HEAV-induced cell death and acetoxymethylester(BAPTA-AM)was used to verify the involvement of cytosolic calcium in this *** calcium measurements were made using *** HEAV decreased the viability of MCF-7,SKBR-3 and K562 cells(Pintracellular Ca^(2+)chelator,decreased the number of non-viable cells treated with HEAV(P<0.05).Conclusions HEAV is cytotoxic and activates several modalities of cell death,which are partially dependent on lysosomal release of Ca^(2+).These effects may be related to artemisinin and caffeoylquinic acids,the main compounds identified in HEAV.

关键词： Artemisia vulgaris necroptosis ferroptosis late apoptosis Ca^(2+)signaling

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