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Monascus vinegar alleviates high-fat-diet-induced inflammation in rats by regulating the NF-κB and PI3K/AKT/mTOR pathways

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Food Science and Human Wellness 2022年第4期11卷 943-953页

作者： Huanmei Meng Jia Song Bingqian Fan Yingqi Li Jiaojiao Zhang Jinping Yu Yu Zheng Min Wang State Key laboratory of Food Nutrition and Safety Key Laboratory of Industrial Fermentation MicrobiologyTianjin Engineering Research Centre of Microbial Metabolism and Fermentation Process ControlCollege of BiotechnologyTianjin University of Science&TechnologyTianjin 300457China Shandong Engineering Research Centre of Condiment Fermentation Technology Shandong Yutu Co.LtdZibo 255300China

Monascus vinegar(MV) is a typical fermented food with various health-promoting effects. This study aimed to evaluate the role of MV in alleviating high-fat-diet-induced inflammation in rats with hyperlipidemia and elucidate the possible regulatory mechanisms. In the study, serum lipid profiles, liver pathology and liver inflammatory cytokines were analyzed in hyperlipidemia rats with MV(0.5 mL/kg mb, 2 mL/kg mb). Results showed that the administration of MV alleviated dyslipidemia by decreasing the serum and liver levels of triglyceride and total cholesterol. Increase in hepatic lipase and carnitine palmitoyl transferase 1(CPT-1)levels and decrease in hepatocyte steatosis, nephritis, and intestinal tissue injury in the HD group showed that high-dose MV can significantly suppress hepatic lipid accumulation and steatosis. In addition, compared with the model(MOD) group, the HD group showed significantly down-regulated the level of serum or hepatic alanine aminotransferase(ALT), aspartate aminotransferase(AST), CPT-1, interleukin(IL)-2, IL-6, IL-12,and tumor necrosis factor α(TNF-α). Moreover, the HD group showed repressed hepatic nuclear factor κB(NF-κB) pathway and inactivated phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt)/mammalian target of rapamycin(mTOR) pathway mitigated liver inflammation. Similar results were obtained from cell experiments. Collectively, these findings revealed that MV might attenuate high-fat-diet-induced inflammation by inhibiting the NF-κB and PI3K/Akt/mTOR pathways.

关键词： Monascus vinegar Hyperlipidemia Inflammation NF-κB PI3K/AKT/mTOR

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Fast Determination of Vitamin B<sub>2</sub>Based on Molecularly Imprinted Electrochemical Sensor

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Engineering（科研） 2012年第10期4卷 129-134页

作者： Peidong Xu Changsheng Qiao Shufeng Yang Lijuan Liu Mingwei Wang Juankun Zhang Key laboratory of industrial microbiology Ministry of Education College of Bioengineering Tianjin Key Laboratory of Industry Microbiology College of Bioengineering Tianjin University of Science & Technology TUST Tianjin P.R.China

Under the condition of weak acidity of pH 5.2, a sensitive vitamin B2 electrochemical sensor based on molecularly imprinted nonconducting polymer of o-aminophenol by potentiostatic polymerization in the presence of template(vitamin B2) on a glassy carbon electrode was prepared, and its performance was studied. The sensor exhibited good sensitivity and selectivity to VB2. The detection limit went down to 2.3851nM, and a linear relationship between the current incremental and the concentration was found in the range of 10~120nM. And the sensor could use in detection of VB2 real sample for a long time and show good reproducibility. The average recovery rate to VB2 was 98.41%.

关键词： Vitamin B2 Molecularly Imprinted Technique Potentiostatic Polymerization O-aminophenol Drug Determination

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Isolation of a salt tolerant laccase secreting strain of Trichoderma sp. NFCCI-2745 and optimization of culture conditions and assessing its efectiveness in treating saline phenolic efuents

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Journal of Environmental Sciences 2013年第12期25卷 2410-2416页

作者： L.M.Divya G.K.Prasanth C.Sadasivan Department of Biotechnology and microbiology Kannur University

Most of the hazardous pollutants are phenolic in nature and persists in the environment. The ability of laccases to oxidize phenolic compounds and reduce molecular oxygen to water has led to intensive studies of these enzymes. Therefore the fungal strains with high laccase activity and substrate affinity that can tolerate harsh environmental conditions have a potential for biotechnological applications. Salt tolerant laccase secreting fungi can be utilized in treatment of saline and phenolic rich industrial effluents such as coir effluent and textile effluent that needed to be diluted several fold before microbial treatment. This is the first study describing the isolation and optimization of a salt tolerant strain of Trichoderma sp. potential for industrial applications. The fungus was identified based on morphological characteristics and was subsequently confirmed with molecular techniques and deposited at National Fungal Culture Collections of India （NFCCI） under the Accession No. Trichoderma viride NFCCI 2745. In contrast to other laccase secreting fungi, light conditions did not exert much influence on laccase production of this strain and salinity enhanced its laccase secretion. The fungus effectively removed the phenolic content of the textile effluent, coir-ret liquor and wood processing effluent within 96 hr of incubation. The tolerance of the fungus to high salinity and phenolic compounds makes this strain ideal for treating saline and phenolic rich industrial effluents.

关键词： laccase optimization phenol tolerant salt tolerant Trichoderma viride

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Is IL1R1 required for celastrol’s leptin-sensitization and antiobesity effects?

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Traditional Medicine Research 2019年第4期4卷 174-177页

作者： Yuan Yao Long Ma State Key laboratory of Food Nutrition and Safety Key Laboratory of Industrial Fermentation Microbiology (Ministry of Education) Tianjin Key Laboratory of Industry Microbiology School of Biotechnology Tianjin University of Science & Technology Tianjin China

The article by Xudong Feng et al.[1], published on 4 March 2019 in the journal of Nature Medicine, showed the relationship between natural product celastrol and obesity. The researchers demonstrated celastrol was able to sensitize leptin and displayed antiobesity effects through IL1R1 (Interleukin-1 receptor 1). It was proved that IL1R1 was a gatekeeper for celastrol’s metabolic actions.

关键词： receptor Is leptin was act

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Formaldehyde Biosensor with Formaldehyde Dehydrogenase Adsorped on Carbon Electrode Modified with Polypyrrole and Carbon Nanotube

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Engineering（科研） 2012年第10期4卷 135-138页

作者： Mingwei Wang Shuhai Jiang Yi Chen Xiang Chen Li Zhao Juankun Zhang Jinfeng Xu Key laboratory of industrial microbiology Ministry of Education College of Biotechnology Tianjin Key Laboratory of Industry Microbiology College of Biotechnology Tianjin University of Science&Technology Tianjin 300457 China

In this study, a carbon electrode electroless polymerization of polypyrrole, which formed a layer of conductive film interface,then absorped a layer of carbon nanotube particles by the way of self-assembled, is studied. The modified electrode is used to electrostatic adherence of formaldehyde dehydrogenase, with Nafion solution capped. Moreover this paper discusses the work of electrode position,? PH value and scanning speed on the influence of the electrode response, the response potential is -1.2v, the optimal PH value is 7.5, and we also review the linear range of this electrode, we discovered taht this biosensor have a good linear relationship with the concentration of 1ug/ml-360ug/ml, the Correlation coefficient is 0.9983. In addition,with the stability of this electrode tested, it can be stored 15d at4°C.

关键词： Formaldehyde Dehydrogenase Eletrostatic Self-assembly Biosensor

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Efficient enzymatic synthesis of(S)-1-(30-bromo-20-methoxyphenyl)ethanol,the key building block of lusutrombopag

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Green Synthesis and Catalysis 2023年第3期4卷 268-271页

作者： Yunfeng Cui Yangyang Ji Xi Chen Jianjiong Li Jinhui Feng Qing Zhao Peiyuan Yao Qiaqing Wu Dunming Zhu National Technology Innovation Center of Synthetic Biology National Engineering Research Center of Industrial Enzymes and Tianjin Engineering Research Center of Biocatalytic TechnologyTianjin Institute of Industrial BiotechnologyChinese Academy of SciencesTianjin 300308China Key laboratory of industrial Fermentation microbiology Ministry of EducationCollege of BiotechnologyTianjin University of Science&TechnologyTianjin 300457China

(S)-1-(30-Bromo-20-methoxyphenyl)ethanol((S)-1b)is the key precursor for the synthesis of *** bioreduction of 1-(30-bromo-20-methoxyphenyl)ethanone(1a)offers an attractive method to access this important *** screening the available carbonyl reductases,we obtained a carbonyl reductase from Novosphingobium aromaticivorans(CBR),which could completely convert 100 g/L of 1a to(S)-***,a carbonyl reductase from Novosphingobium ***2(NoCR)was identified to completely convert 200 g/L of 1a to(S)-1b with excellent enantioselectivity(>99%ee)and 77%isolated yield using FDH/formate system for NADH *** K_(m) and k_(cat) of recombinant NoCR towards 1a were 0.66 mmol/L and 7.5 s-1,and the catalytic efficiency k_(cat)/K_(m) was 11.3 mmol/***,NoCR showed high catalytic activity and stereoselectivity towards acetophenone derivatives with halogen or methoxy substitution on the benzene ring,indicating that NoCR is a valuable biocatalyst with potential practical applications.

关键词： Biocatalysis Carbonyl reductase Asymmetric reduction Chiral aryl alcohols Lusutrombopag

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OGT Mediated Histone H2B S112 Glc NAcylation Regulates DNA Damage Response

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Journal of Genetics and Genomics 2015年第9期42卷 467-475页

作者： Panfei Wang Changmin Peng Xia Liu Hailong Liu Yali Chen Li Zheng Baolin Han Huadong Pei Key laboratory of industrial Fermentation microbiology Ministry of Education Tianjin Industrial Microbiology Key Lab College of BiotechnologyTianjin University of Science and Technology State Key laboratory of Proteomics Beijing Proteome Research Center Beijing Institute of Radiation Medicine School of Nursing and Medical Technology Jianghan University Department of Radiotherapy Tianjin Baodi Hospital Baodi Clinical College of Tianjin Medical University

O-GlcNAcylation is an important post-translational modification and has been implicated in many fundamental cellular processes. Recent studies showed that O-linked N-acetylglucosamine （GlcNAc） transferase （OGT） mediated O-GlcNAcylation of histone H2B Ser 112 （H2B S 112 GlcNAcylation） plays an important role in gene transcription. However, the role of this histone modification in DNA damage response has not been studied yet. In this study, we found that OGT and OGT mediated H2B S112 GlcNAcylation are involved in DNA damage response for maintaining genomic stability and are required for resistance to many DNA-damaging and replication stress- inducing agents. OGT mediated H2B Sl12 GlcNAcylation increased locally upon the induction of double-strand breaks （DSBs）, and depletion of OGT or overexpression of H2B S 112A mutant impaired homologous recombination （HR） and nonhomologous end-joining （NHEJ）. Mechanistically, H2B Sl12 GlcNAcylation could bind Nijmegen breakage syndrome 1 （NBS1） and regulate NBS1 foci for- mation. Taken together, our results demonstrate a new function of histone O-GlcNAcylation in DNA damage response （DDR）.

关键词： OGT H2B S112 GlcNAcylation DNA damage response NBSI

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Optimization of a fermented pumpkin-based beverage to improve Lactobacillus mali survival and-glucosidase inhibitory activity:A response surface methodology approach

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Food Science and Human Wellness 2018年第1期7卷 57-70页

作者： W.Y.Koh U.Uthumporn A.Rosma A.R.Irfan Y.H.Park Food Technology Division School of Industrial TechnologyUniversiti Sains MalaysiaMinden 11800PenangMalaysia Bioprocess Division School of Industrial TechnologyUniversiti Sains MalaysiaMinden 11800PenangMalaysia Department of Applied microbiology and Biotechnology Yeungnam UniversityGyeongsanSouth Korea

The aim of this research was to develop an optimum fermentation and composition model for a new fermented pumpkin-based beverage with high probiotic survival and-glucosidase inhibitory *** between fermentation temperature,inoculum and ingredient concentration with response variables(fermentation time at the fermentation endpoint pH 4.5,survival rate of Lactobacillus mali K8 in pumpkin-based beverage treated with simulated gastrointestinal tract enzyme fluids,-glucosidase inhibitory activity and sensory overall acceptability after 4 weeks of refrigerated storage)was investigated using response surface *** formulation was obtained at an approximation of 40%pumpkin puree concentration,8 Log CFU/mL inoculum and at 35℃.The product derived from this optimum formula reached the fermentation endpoint after 28.34±0.10 h and the quality change during 4 weeks storage was *** product achieved 88.56±0.67%of *** survival after treatment with simulated gastric and intestinal juices;demonstrated 95.89±0.30% α-glucosidase inhibitory activity,as well as scored 6.99±0.40 on sensory overall acceptability after 4 weeks of *** findings illustrated that the model is effective in improving probiotic survival and α-glucosidase inhibitory activity with excellent sensory acceptability,thus may offer a dietary means for the management of hyperglycaemia.

关键词： Probiotics Response surface methodology Box-Behnken Hyperglycaemia Functional food

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An Amperometric Sensor for Sunset Yellow FCF Detection Based on Molecularly Imprinted Polypyrrole

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Engineering（科研） 2012年第10期4卷 159-162页

作者： Jinfeng Xu Yi Zhang Hao Zhou Mingwei Wang Peidong Xu Juankun Zhang Key laboratory of industrial Fermentation microbiology Ministry of Education College of Biotechnology Tianjin Key Lab of Industrial Microbiology College of BiotechnologyTianjin University of Science and Technology Tianjin 300457 People’s Republic of China

An electrochemical method for fast detecting the concentration of sunset yellow FCF in wine samples was developed in this study. The sensor based on imprinted films which fabricated by electropolymerization of pyrrole on a glassy carbon electrode in the presence of sunset yellow FCF as the template. Comparing to the polypyrrole non-imprinted modified (NIP) electrode, the polypyrrole molecularly imprinted polymer (MIP) electrode improved the electrochemical performance of the sensor significantly. The peak current at about 0.26 V was linear with the concentration of sunset yellow FCF from 0.4 to 2 μM and from 2 to 8 μM. It can be used for more than 10 times to maintain a stable response result. The sensor had the good selectivity on sunset yellow FCF, amaranth and tartrazine, which the selection factors were 1.00, 0.80 and 0.85,respectively.

关键词： Electrochemical Sensor Polypyrrole Sunset Yellow FCF Molecularly Imprinted Polymers

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Effects of some common additives on the antimicrobial activities of alcohol-based hand sanitizers

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Asian Pacific Journal of Tropical Medicine 2018年第3期11卷 222-226页

作者： Nzekwe Ifeanyi Thaddeus Egbuna Chukwuemeka Francis Okpara Ogonna Jane Agubata Chukwuma Obumneme Esimone Charles Okechukwu Department of Pharmaceutics & Pharmaceutical Technology Nnamdi Azikiwe University Department of Pharmaceutical Technology & industrial Pharmacy University of Nigeria Department of Pharmaceutical microbiology and Biotechnology Nnamdi Azikiwe University

Objective: To study the effects of some common additives on the antimicrobial activities of alcohol-based hand sanitizers. Methods: The antibacterial activities of varying aqueous concentrations of ethanol and isopropyl alcohol were tested by the agar well diffusion method. The influences of different concentrations of glycerin was similarly tested. Finally, isopropyl alcohol and benzalkonium chloride were combined in different ratios within the sate use concentrations of each, and the effects of these combinations were compared with values obtained for the two agents used alone. Statistical methods, such as student t test and one-way ANOVA were used when appropriate to evaluate the differences in activity. Results: The activities of the alcohols showed marked concentration dependence, and both showed peak activity at 85%-95% concentration range. Over the concentration range of 60%-100%, isopropyl alcohol inhibited more bacterial and fungal organisms than ethanol, though the inhibition zone diameters it produced were not statistically different from those of ethanol for organisms which were sensitive to both of *** of glycerin reduced the antimicrobial activities of the isopropyl alcohol, as shown by reduction in the inhibition zone diameters produced in vitro, which may be due to reduced drug diffusion with increase in viscosity. Addition of benzalkonium to isopropyl alcohol systems improved the activity of the alcohol, but the overall activity of the combination was not superior to that seen in the use of benzalkonium alone. Conclusion: Alcohol-based hand sanitizers should not be used outside the concentration range of 85%-95% and isopropyl alcohol inhibits more bacterial and fungal organisms than ethanol for most concentrations. Inclusion of benzalkonium improves the antimicrobial spectrum and activity of isopropyl alcohol, and the combination may justifiably be used to achieve both immediate and long lasting effect. Glycerin may adversely affect the an

关键词： Infection control Disinfection Hand sanitizer Alcohol Optimization

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