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Bioethanol Production from Molasses by Yeasts with Stress-Tolerance Isolated from Aquatic Environments in Japan

Advances in microbiology 2019年第12期9卷 1000-1011页

作者： Yuka Naito Masahiko Okai Masami Ishida Masachika Takashio Naoto Urano laboratory of Marine Biochemistry Department of Ocean Sciences Tokyo University of Marine Science and Technology Konan Minato-ku Tokyo Japan Zensho Laboratories of Food Technology Zensho Holdings Co. Ltd. Konan Minato-ku Tokyo Japan

Bioethanol is a safe and renewable source of energy that continues to be a research focus, since fossil fuels have been linked to global warming and nuclear energy sources are affected by the increased safety concerns following the 2011 nuclear power plant accident in Japan. In general, bioethanol is converted from a biomass by yeast fermentation. The production efficiency of this bioethanol is not sufficiently high, and its practical use as a substitute for fossil fuels and nuclear energy is thus limited. For the industrial production of bioethanol, the yeast fermentation of biomass cultures containing high concentration sugar, NaCl, and ethanol is necessary, but this might induce phenomena in which the stresses arising in the yeasts weaken their cells during fermentation. As described herein, we isolated 1028 strains of yeasts from natural aquatic environments: Japan’s Tama River and Lake Kasumigaura. Among them, 412 strains were fermentative yeasts and 31 strains showed high fermentation ability under a 30% sorbitol + 10% ethanol condition. These strains were identified as Torulaspola delbrueckii, Wickerhamomyces anomalus, Candida glabrata, Pichia kudriavzevii, Saccharomyces cf. cerevisiae/paradoxus, and Lachancea kluyveri. The strains T. delbrueckii, W. anomalus, and C. glabrata also showed tolerance against 15% NaCl. Most importantly, S. cf. cerevisiae/paradoxus H28 and L. kluyveri F2-67 produced 57.4 g/L and 53.9 g/L ethanol from molasses (sucrose 104.0 g/L, fructose 33.4 g/L, and glucose 24.8 g/L) within 48 hrs at 25°C, respectively.

关键词： Stress-Tolerance Molasses Yeast Species Aquatic Environment Fermentation

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Isolating,screening and biochemical characterization of a novel thermal Fungal polygalacturonase from Penicillium oxalicum

Isolating,screening and biochemical characterization of a no...

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The Ninth National Symposium on Enzyme Engineering

作者： Cheng Zhong Lu Bo Life Science and Technology College Guangxi University State Key laboratory of Non-food Biomass Energy and Enzyme Technology National Engineering Research Center for Non-food BiorefineryGuangxi Academy of Sciences

＜正＞Pectins are a heterogeneous group of high molecular weight complex,acidic structural polysaccharides which consist largely of D-galactopyranosyluronic *** are a heterogeneous group of related enzymes that hydrolyze the pectic *** enzymes have been industrially produced by the filamentous fungi and are among the most important industrial ***（PG）is the main

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Improving ITS sequence data for identification of plant pathogenic fungi

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Fungal Diversity 2014年第4期67卷 11-19页

作者： R.Henrik Nilsson Kevin D.Hyde Julia Pawlowska Martin Ryberg Leho Tedersoo Anders Bjornsgard Aas Siti A.Alias Artur Alves Cajsa Lisa Anderson Alexandre Antonelli A.Elizabeth Arnold Barbara Bahnmann Mohammad Bahram Johan Bengtsson-Palme Anna Berlin Sara Branco Putarak Chomnunti Asha Dissanayake Rein Drenkhan Hanna Friberg Tobias Guldberg Froslev Bettina Halwachs Martin Hartmann Beatrice Henricot Ruvishika Jayawardena Ari Jumpponen Havard Kauserud Sonja Koskela Tomasz Kulik Kare Liimatainen Bjorn D.Lindahl Daniel Lindner Jian-Kui Liu Sajeewa Maharachchikumbura Dimuthu Manamgoda Svante Martinsson Maria Alice Neves Tuula Niskanen Stephan Nylinder Olinto Liparini Pereira Danilo Batista Pinho Teresita M.Porter Valentin Queloz Taavi Riit Marisol Sánchez-García Filipe de Sousa Emil Stefańczyk Mariusz Tadych Susumu Takamatsu Qing Tian Dhanushka Udayanga Martin Unterseher Zheng Wang Saowanee Wikee Jiye Yan Ellen Larsson Karl-Henrik Larsson Urmas Koljalg Kessy Abarenkov Department of Biological and Environmental Sciences University of GothenburgBox 46140530 GothenburgSweden Institute of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand Department of Plant Systematics and Geography Faculty of BiologyUniversity of WarsawAl.Ujazdowskie 400-478 WarsawPoland Department of Organismal Biology Uppsala UniversityNorbyvagen 18D75236 UppsalaSweden Institute of Ecology and Earth Sciences University of TartuLai 40Tartu 51005Estonia Microbial Evolution Research Group University of OsloBlindernveien 310371 OsloNorway Institute of Biological Sciences University of Malaya50603 Kuala LumpurMalaysia Department of Biology CESAMUniversity of Aveiro3810-193 AveiroPortugal School of Plant Sciences The University of Arizona1140 E South Campus DriveForbes 303TucsonAZ 85721USA laboratory of Environmental microbiology Institute of Microbiology ASCRVídeňská108314220 Prague 4Czech Republic Department of Infectious Diseases Institute of BiomedicineSahlgrenska AcademyUniversity of GothenburgGuldhedsgatan 1041346 GoteborgSweden Department of Forest Mycology and Plant Pathology Swedish University of Agricultural SciencesBox 702675007 UppsalaSweden University of California at Berkeley 321 Koshland Hall University of CaliforniaBerkeleyCA 94720-3102USA Agriculture and Forestry Sciences Beijing 100097China Institute of Forestry and Rural Engineering Estonian University of Life SciencesKreutzwaldi551014 TartuEstonia Natural History Museum of Denmark Øster Voldgade 5-71350 København KDenmark Institute for Genomics and Bioinformatics Graz University of Technology8010 GrazAustria Core Facility Bioinformatics Austrian Centre of Industrial Biotechnology8010 GrazAustria Forest Soils and Biogeochemistry Swiss Federal Research Institute WSLZuercherstrasse 1118903 BirmensdorfSwitzerland Molecular Ecology Institute for Sustainability SciencesAgroscopeReckenholzstrasse 191

Plant pathogenic fungi are a large and diverse assemblage of eukaryotes with substantial impacts on natural ecosystems and human *** taxa often have complex and poorly understood life cycles,lack observable,discriminatory morphological characters,and may not be amenable to in vitro *** a result,species identification is frequently ***(DNA sequence)data have emerged as crucial information for the taxonomic identification of plant pathogenic fungi,with the nuclear ribosomal internal transcribed spacer(ITS)region being the most popular ***,international nucleotide sequence databases are accumulating numerous sequences of compromised or low-resolution taxonomic annotations and substandard technical quality,making their use in the molecular identification of plant pathogenic fungi *** we report on a concerted effort to identify high-quality reference sequences for various plant pathogenic fungi and to re-annotate incorrectly or insufficiently annotated public ITS sequences from these fungal lineages.A third objective was to enrich the sequences with geographical and ecological *** results-a total of 31,954 changes-are incorporated in and made available through the UNITE database for molecular identification of fungi(http://***),including standalone FASTA files of sequence data for local BLAST searches,use in the next-generation sequencing analysis platforms QIIME and mothur,and related *** present initiative is just a beginning to cover the wide spectrum of plant pathogenic fungi,and we invite all researchers with pertinent expertise to join the annotation effort.

关键词： Phytopathogenic fungi Molecular identification ITS Taxonomy Annotation

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Self-fusion of protoplasts enhances cellulase production in Trichoderma reesei

Self-fusion of protoplasts enhances cellulase production in ...

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The Ninth National Symposium on Enzyme Engineering

作者： Jun Huang Dong Chen Ribo Huang State Key laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangxi University College of Life Science and Technology Guangxi University National Engineering Research Center for Non-Food Biorefinery Guangxi Academy of Sciences

Genome shuffling is a powerful strategy for rapid engineering of microbial strains for desirable industrial *** have developed a shuffled strain of Trichoderma reesei by recursive protoplast *** initial mutant populations were generated by ultraviolet（UV）irradiation and nitrosoguanidine（NTG）.Protoplasts were isolated from mutant strain using lysing enzymes and self-fusion of protoplasts was carried out using polyethylene glycol in STC *** fused protoplasts of *** were regenerated and1000 self-fusants were selected to study the cellulase production.A recombinant S4-27,was obtained after four rounds ofprotoplast fusion,and its production of cellulase reached 0.5 IU/1,which was increased by 153%in comparison with that of the original strain ***,ethanol（upto 4%v/v）did not repress enzyme production by the S4-27 under fermentation *** distinction of the mutant was revealed by the presence of two unique amplicans in comparative DNA fingerprinting performed using 20 random primers.

关键词： Trichoderma reesei Protoplast fusion cellulase RAPD

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Improved Method for Isolation of Microbial RNA from Biofuel Feedstock for Metatranscriptomics

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Advances in microbiology 2013年第1期3卷 101-107页

作者： Hailan Piao Lye Meng Markillie David E. Culley Roderick I. Mackie Matthias Hess Department of Animal Sciences Institute for Genomic Biology and Energy Biosciences University of Illinois Urbana USA DOE Joint Genome Institute Walnut Creek USA Pacific Northwest National laboratory Richland USA School of Molecular Biosciences Washington State University Richland USA

Metatranscriptomics—gene express profiling via DNA sequencing—is a powerful tool to identify genes that are actively expressed and might contribute to the phenotype of individual organisms or the phenome (the sum of several phenotypes) of a microbial community. Furthermore, metatranscriptome studies can result in extensive catalogues of genes that encode for enzymes of industrial relevance. In both cases, a major challenge for generating a high quality metatranscriptome is the extreme lability of RNA and its susceptibility to ubiquitous RNAses. The microbial community (the microbiome) of the cow rumen efficiently degrades lignocelullosic biomass, generates significant amounts of methane, a greenhouse gas twenty times more potent than carbon dioxide, and is of general importance for the physiological wellbeing of the host animal. Metatranscriptomes of the rumen microbiome from animals kept under different conditions and from various types of rumen-incubated biomass can be expected to provide new insights into these highly interesting phenotypes and subsequently provide the framework for an enhanced understanding of this socioeconomically important ecosystem. The ability to isolate large amounts of intact RNA will significantly facilitate accurate transcript annotation and expression profiling. Here we report a method that combines mechanical disruption with chemical homogenization of the sample material and consistently yields 1 mg of intact RNA from 1 g of rumen-incubated biofuel feedstock. The yield of total RNA obtained with our method exceeds the RNA yield achieved with previously reported isolation techniques, which renders RNA isolated with the method presented here as an ideal starting material for metatranscriptomic analyses and other molecular biology applications that require significant amounts of starting material.

关键词： RNA Extraction Biofuels CAZymes Metatranscriptomics Systems microbiology Microbial Ecology

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Nitrate removal from groundwater using chemically modified coconut husk based granular activated carbon:characterization of the adsorbent,kinetics and mechanism

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Systems microbiology and Biomanufacturing 2023年第2期3卷 370-383页

作者： Solomon K.M.Huno Jewel Das Eric Dvan Hullebusch Ajit P.Annachhatre Eldon R.Rene Pollution Prevention and Resource Recovery Chair Group Department of Water SupplySanitation and Environmental EngineeringIHE Delft Institute of Water EducationP.O.Box 30152601 DA DelftThe Netherlands Environmental Engineering and Management Department of EnvironmentEnergy and Climate ChangeSchool of EnvironmentResource and DevelopmentAsian Institute of Technology58 Moo 9Km.42Paholyothin HighwayP.O.Box 4Klong Luang 12120Pathum ThaniThailand National University of Ireland Galway University RoadGalway H91 TK33Ireland Bangladesh Council of Scientific and industrial Research(BCSIR) BCSIR Laboratories ChattogramChattogram 4220Bangladesh Universite Paris Cite Institut de Physique du Globe de ParisCNRSF‑75005 ParisFrance School of Engineering Indian Institute of Technology MandiA4‑209MandiHimachal Pradesh 175001India

In this study,a highly porous chemically activated granular activated carbon(GAC)was prepared from coconut husk and tested as an adsorbent to remove nitrate from contaminated *** prepared GAC was characterized by Fouriertransform infrared spectroscopy(FTIR),thermogravimetric and differential thermal analysis(TGA/DTA),scanning electron microscopy(SEM)and the Brunauer-Emmett-Teller(BET)surface area(SBET)*** effects of various process parameters such as initial nitrate concentration,contact time and adsorbent dose on nitrate removal efficiency(response)by the modified GAC were investigated using the statistically significant response surface methodology and Box-Behnken design of *** experimental data were fitted to well-known adsorption isotherms and kinetic models to ascertain the mechanism of the adsorption *** of variance(ANOVA)was performed to determine the significance of the individual and the interactive effects of process variables on the *** BET surface area(SBET)and micropore volume of the prepared GAC from coconut husk was 1120 m^(2)/g and 0.392 cm^(3)/g,*** experimental results showed that physisorption was the main adsorption mechanism governing the process,while the rate of adsorption was limited at initial nitrate concentrations>10 mg/*** Langmuir mono-layer adsorption isotherm best fitted the experimental data with a maximum adsorption capacity of 6.0±1.3 mg/g(~92.5%)with an adsorbent dose of 0.1 g/50 mL,an equilibrium time of 6 h at 28±2℃,and at pH 7.6(±0.2).Among the tested process variables,the adsorbent dose and initial nitrate concentration showed significant effects on the nitrate removal efficiency.

关键词： Nitrate adsorption Groundwater Granular activated carbon Response surface methodology(RSM) Box-Behnken Coconut Husk

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Profiling proteomic responses to hexokinase-II depletion in terpene-producing Saccharomyces cerevisiae

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Engineering microbiology 2023年第3期3卷 1-13页

作者： Zeyu Lu Qianyi Shen Lian Liu Gert Talbo Robert Speight Matt Trau Geoff Dumsday Christopher B.Howard Claudia E.Vickers Bingyin Peng ARC Centre of Excellence in Synthetic Biology Queensland University of TechnologyBrisbaneQLD4000Australia Australian Institute for Bioengineering and Nanotechnology(AIBN) The University of QueenslandBrisbaneQLD4072Australia Centre of Agriculture and the Bioeconomy School of Biology and Environmental ScienceFaculty of ScienceQueensland University of TechnologyBrisbaneQLD4000Australia CSIRO Synthetic Biology Future Science Platform Commonwealth Scientific and Industrial Research Organisation(CSIRO)Black MountainACT2601Australia School of Chemistry and Molecular Biosciences(SCMB) the University of QueenslandBrisbaneQLD4072Australia Metabolomics Australia(Queensland Node) Australian Institute for Bioengineering and Nanotechnology(AIBN)The University of QueenslandBrisbaneQLD4072Australia CSIRO Manufacturing ClaytonVIC3169Australia Eden Brew Pty Ltd GlenorieNSW2157Australia

Hexokinase II(Hxk2)is a master protein in glucose-mediated transcriptional repression signaling ***-grading Hxk2 through an auxin-inducible protein degradation previously doubled sesquiterpene(nerolidol)pro-duction at gram-per-liter levels in Saccharomyces *** transcriptomics/proteomics profiles in Hxk2-deficient background are important to understanding genetic and molecular mechanisms for improved nerolidol production and guiding further strain ***,proteomic responses to Hxk2 depletion are investi-gated in the yeast strains harboring a GAL promoters-controlled nerolidol synthetic pathway,at the exponential and ethanol growth phases and in GAL80-wildtype and gal80Δ*** metabolic pathways and amino acid metabolic pathways show diversified responses to Hxk2 depletion and growth on ethanol,including upregulation of alternative carbon catabolism and respiration as well as downregulation of amino acid ***-repression of GAL genes may contribute to improved nerolidol production in Hxk2-depleted ***-teen transcription factors associated with upregulated genes are *** Ash1-mediated repression on the RIM4 promoter shows the variation on the regulatory effects of different Ash1-binding sites and the syner-gistic effect of Ash1 and Hxk2-mediated *** validation of individual promoters shows that HXT1 promoter activities are glucose-dependent in hxk2Δbackground,but much weaker than those in HXK2-wildtype *** summary,inactivating HXK2 may relieve glucose repression on respiration and GAL promoters for improved bioproduction under aerobic conditions in *** proteomics profiles provide a better genetics overview for a better metabolic engineering design in Hxk2-deficient backgrounds.

关键词： Proteomics Glucose repression Metabolic engineering Crabtree effect Sesquiterpene Synthetic biology Genetic regulation

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Biotechnological Potential of Carotenoids Produced by Extremophilic Microorganisms and Application Prospects for the Cosmetics Industry

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Advances in microbiology 2020年第8期10卷 397-410页

作者： Tayane de Cássia Dias Mendes-Silva Rosileide Fontenele da Silva Andrade Marcio Akio Ootani Paulo Vitor Dias Mendes Rafael Artur de Queiroz Cavalcanti de Sá Milena Roberta Freire da Silva Karolayne Silva Souza Maria Tereza dos Santos Correia Márcia Vanusa da Silva Maria Betânia Melo de Oliveira Department of Biochemistry Federal University of Pernambuco Recife Brazil National Program Postdoctoral CAPES (PNPD) Catholic University of Pernambuco Recife Brazil Bioprocess laboratory Center of Strategic Technologies Northeast Recife Brazil Design Department Federal University of Pernambuco Recife Brazil Nucleus of Bioprospecting and Conservation of the Caatinga Semiarid National Institute/Ministry of Science Technology Innovations and Communications Campina Grande Brazil

Carotenoids are isoprenoid pigments used in food, chemical, textile, pharmaceutical, and cosmetic industries. They act not only as dyes and provitamins A but also have antioxidants, photoprotective, antimicrobial properties, among others. This class of pigment can be obtained traditionally by plants or chemical synthesis, but they have some disadvantages. In recent years, search for alternative sources has been an important strategy for the carotenoid industries. Microbial synthesis is an alternative that has shown good yields, speed, and reduced production costs. Hostile environments, such as the Caatinga domain, represent an interesting source of microorganisms that produce biomolecules, especially carotenoids, because of oxidative stress caused by sunlight. Thus, this region has been attracting the attention of the scientific community and industry for the use of these organisms in the production of carotenoids and applications in cosmetic products;since these compounds have interesting antioxidant and photoprotective properties. In this review, general characteristics of carotenoids, sources of production, industrial applicability, and commercialization will be discussed, as well as perspectives on the production of carotenoids from microorganisms isolated from the Caatinga and their application in anti-UV products.

关键词： Natural Pigment Microbial Production Hostile Environments Antioxidant Photoprotection

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Nat Chem Biol:利用CRISPR-Cas9激活细菌中沉默的基因簇,有望发现新的药物

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现代生物医学进展 2017年第17期17卷 I0004-I0004页

作者： Mingzi M Zhang, Wan Lin Yeo, Ee Lui Ang, Huimin Zhao Fong Tian Wong, Elena Heng Yajie Wang, Shangwen Luo, Ryan E Cobb, Behnam Enghiad, Huimin Zhao Yee Hwee Lim Metabolic Engineering Research laboratory Science and Engineering Institutes Agency for Science Technology and Research (A*STAR) Singapore Molecular Engineering Lab Biomedical Science Institutes A*STAR Singapore Department of Chemical and Biomolecular Engineering University of Illinois at Urbana-Champaign Urbana Illinois USA Organic Chemistry Institute of Chemical and Engineering Sciences A*STAR Singapore.

为了抵抗疾病，很多医药库中的武器是从细菌当中获得的。如今，利用CRISPR-Cas9基因编辑技术。研究人员揭示出沉默基因中隐藏着的更多潜在的宝藏。作为一类常见的细菌。链霉菌被用来产生很多作为抗生素、抗癌试剂和其他药物的化合物。... 详细信息

为了抵抗疾病，很多医药库中的武器是从细菌当中获得的。如今，利用CRISPR-Cas9基因编辑技术。研究人员揭示出沉默基因中隐藏着的更多潜在的宝藏。作为一类常见的细菌。链霉菌被用来产生很多作为抗生素、抗癌试剂和其他药物的化合物。在一项新的研究中。来自美国伊利诺伊大学和新加坡科技研究局的研究人员利用CRISPR-Cas9技术激活链霉菌中不表达的或者说沉默的基因簇。

关键词：基因簇 Chem 细菌利用激活药物 Nat 编辑技术

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