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Ecological damage of submerged macrophyte Myriophyllum spicatum by cell extracts from microcystin(MC)-and non-MC-producing cyanobacteria,Microcystis

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Journal of Oceanology and Limnology 2022年第5期40卷 1732-1749页

作者： Yunni GAO Hui YANG Xiaofei GAO Mei LI Man ZHANG Jing DONG Jingxiao ZHANG Longfei LI Xuejun LI Michele A BURFORD Engineering Lab of Henan Province for Aquatic animal Disease Control Engineering Technology Research Center of Henan Province for Aquatic Animal CultivationCollege of FisheriesHenan Normal UniversityXinxiang 453007China Australian Rivers Institute Griffi th UniversityNathanQueensland 4111Australia

To explore how decomposed Microcystis-dominant cyanobacterial blooms affect submerged macrophytes,the submerged plant Myriophyllum spicatum was exposed to cell extracts from microcystin(MC)-and non-MC-producing Microcystis strains in a laboratory *** showed that both Mcracystis cell extracts exerted obvious damages to plant biomass,photosynthesis,primary and secondary metabolism measures,and resistance of plant antioxidant systems,with MC-producing Microcystis having stronger effects due to the presence of *** other than MCs responsible for the negative effects from both strains needs further *** Shannon diversity and Chao1 indices of epiphytic and planktonic bacteria were decreased by the cell extracts from both Microcystis ***,epiphytic and planktonic bacterial communities responded differently to cell extracts at the genus *** dominant genera of planktonic bacteria including Enterobacter,Pseudomonas,and Novosphingobium from phylum Proteobacteria,Chryseobacterium from phylum Bacteroidetes,and Microbacterium from Actinobacteriota in the treatments with cell extracts were previously reported to have strains with algicidal and MC-degrading capabilities.B acterial genes associated with energy production and conversion,amino acid transport and metabolism,and inorganic ion transport and metabolism,were more abundant in both treatments than the control for planktonic bacteria,but less abundant for epiphytic *** speculate that planktonic bacterial communities have the potential to use and degrade substances derived from Microcystis cell extracts,which may be beneficial for *** to alleviate damages from *** research is needed to verify the structure and function dynamics of epiphytic and planktonic bacteria in the interaction between cyanobacteria and submerged macrophytes.

关键词： microcystins Microcystis Myriophyllum spicatum dissolved organic carbon epiphytic and planktonic bacteria

The Midgut Bacterial Flora of laboratory-Reared Hard Ticks, Haemaphysalis longicornis, Hyalomma asiaticum, and Rhipicephalus haemaphysaloides

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Journal of Integrative Agriculture 2014年第8期13卷 1766-1771页

作者： LI Chun-hong CAO Jie ZHOU Yong-zhi ZHANG Hou-shuang GONG Hai-yan ZHOU Jin-lin Key laboratory of animal Parasitology Ministry of Agriculture/Shanghai Veterinary Research Institute Chinese Academy of Agricultural Sciences

Ixodid ticks play an important role in the transmission of a variety of zoonoses of viral, bacterial and protozoan origin, and they also harbor a wealth of microorganisms. To gain more detailed insights into the potential interactions between bacterial flora and tick-borne pathogens, we investigated the midgut bacterial flora of laboratory-reared Haemaphysalis longicornis, Hyalomma asiaticum and Rhipicephalus haemaphysaloides. Based on morphological, biochemical, and 16 S rDNA sequencing results, we identified 15 species belonging to 12 genera in the midgut of the three ticks. The bacterial communities were similar to those found in other studies of hematophagous arthropods. Kocuria sp. was the most frequently isolated species and its 16 S rDNA gene sequence was very similar to Kocuria koreensis P31 T. To our knowledge, this is the first report of the bacterial flora of tick midguts and the results show that there were many different bacterial species in each tick species. Among the most common genera, there may have been a novel species in the genus Kocuria. The results might be the first step for looking for different aspects of the pathogen and tick interaction.

关键词： bacteria Kocuria sp. tick midgut

Mutual interaction between gut microbiota and protein/amino acid metabolism for host mucosal immunity and health

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animal Nutrition 2021年第1期7卷 11-16页

作者： Liuting Wu Zhiru Tang Huiyuan Chen Zhongxiang Ren Qi Ding Kaiyang Liang Zhihong Sun laboratory for Bio-feed and Molecular Nutrition College of Animal Science and TechnologySouthwest UniversityChongqing400715China

In recent years,many studies have shown that the intestinal microflora has various effects that are linked to the critical physiological functions and pathological systems of the *** intestinal microbial community is widely involved in the metabolism of food components such as protein,which is one of the essential nutrients in ***,dietary protein/amino acids have been shown to have had a profound impact on profile and operation of gut *** review summarizes the current liter-ature on the mutual interaction between intestinal microbiota and protein/amino acid metabolism for host mucosal immunity and health.

关键词： Gut microbiota Amino acid Metabolism Mucosal immunity Host

interaction of hispidin with pepsin:Multi-spectroscopic analyses and docking simulation

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Food and Health 2024年第1期6卷 21-27页

作者： Wen-Qiang Wang Si-Hua Fan Xin-Huai Zhao Li Wang Ming-Hui Zhang Qiang Zhang Xue-Jun Gao College of Biology and Food Engineering Guangdong University of Petrochemical TechnologyMaoming525000China College of animal Science Yangtze UniversityJingzhou 434023China

Hispidin is a pyranone compound found in edible and medicinal mushrooms of the Phellinus and Inonotus *** investigation used fluorescence spectroscopy,UV absorption spectroscopy,and molecular docking to examine the interaction of hispidin with *** Stern-Volmer method was used to perform the fluorescence quenching measurements at different temperatures(298 K,303 K,and 310 K).According to the findings,hispidin induced a static quenching mechanism in pepsin that resulted in the creation of a hispidin-pepsin complex with binding constants(Ka)ranging from 9.56×10^(4) to 3.45×10^(5) L mol^(-1).The positive values ofΔH(84.6 kJ mol-1)andΔS(337.9 J mol^(-1) K^(-1))demonstrated that hydrophobic forces contributed to forming the hispidin-pepsin *** findings of UV-vis absorption,synchronous fluorescence,and 3D fluorescence spectraspectra demonstrated that hispidin altered the conformation and microenvironment of *** to the analysis of molecular docking,hispidin got into the pepsin's active *** research clarifies the molecular mechanisms by which hispidin binds to pepsin and helps understand its possible biological activity in vivo.

关键词： hispidin pepsin spectroscopy molecular docking

Effects of metabolizable energy and crude protein levels on laying performance,egg quality and serum biochemical indices of Fengda-1 layers

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animal Nutrition 2016年第2期2卷 93-98页

作者： Yang Ding Xingchen Bu Nannan Zhang Lanlan Li Xiaoting Zou Key laboratory of animal Nutrition and Feed Science Ministry of Agriculture Zhejiang Provincial Laboratory of Feed and Animal Nutrition Feed Science Institute Zhejiang University

This study was conducted to investigate the effects of dietary ME and CP levels on laying performance, egg quality and serum biochemical indices of Fengda-1 layers. In a 2 × 3 factorial arrangement, 2,400 Fengda-1 layers(32 wk of age) were randomly assigned to 6 experimental diets with 2,650 and 2,750 kcal of ME/kg of diet, each containing 14.50%, 15.00% and 15.50% CP, respectively. Each dietary treatment was replicated 5 times, and feed and water were provided ad libitum. The trial lasted for 10 wk, including a2-week acclimation period and an 8-week experimental period. Our results showed that ADFI decreased as the ME level of diet increased from 2,650 to 2,750 kcal/kg(Pinteraction between ME and CP on laying performance, egg quality, or serum biochemical indices(P > 0.05). Based on the data under the experimental conditions, the optimal dietary ME and CP levels of Fengda-1 layers are 2,650 kcal/kg and 15.00%(33 to 41 wk of age).

关键词： Energy Protein Laying performance Egg quality Fengda-1 layers

Involvement of 2'-5'oligoadenylate synthetase-like proteinin the survivalof Mycobacterium tuberculosis avirulent strain in macrophages

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animal Diseases 2023年第4期3卷 275-285页

作者： Aikebaier Reheman Xiaojian Cao Yifan Wang Xi Nie Gang Cao Wei Zhou Bing Yang Yingying Lei Weipan Zhang Muhammad Ahsan Naeem Xi Chen State Key laboratory of Agricultural Microbiology College of Veterinary MedicineHuazhong Agricultural UniversityWuhan 430070China College of animal Science and Technology Tarim UniversityAlar 843300China Bio-Medical Center Huazhong Agricultural UniversityWuhan 430070China Department of Basic Sciences(Pharmacology) University of Veterinary and Animal Sciences(Narowal Campus)Lahore 54000Pakistan

Mycobacterium tuberculosis(***)can replicate in the macrophage by interfering with many host protein *** it is far from known these host proteins for controlling *** ***,we infected macrophages including THP-1 and Raw264.7 cells with *** and identified the differentially expressed genes(DEGs)in the interferon signaling *** them,2'-5'oligoadenylate synthetase-like(OASL)underwent the greatest upregulation in ***-infected *** of the expression of OASL attenuated *** survival in ***,bioinformatics analysis revealed the potential interaction axis of OASL-TAB3-RvO127,which was further validated by the yeast-two-hybrid(Y2H)assay and *** interaction axis might regulate the *** survival and proliferation in *** study reveals a possible role of OASL during *** infection as a target to control its propagation.

关键词： Mycobacterium tuberculosis Interferon Interferon stimulated genes OASL

MiR-743a-5p regulates differentiation of myoblast by targeting Mob1b in skeletal muscle development and regeneration

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Genes & Diseases 2022年第4期9卷 1038-1048页

作者： YongSheng Zhang YiLong Yao ZiShuai Wang Dan Lu YuanYuan Zhang Adeyinka Abiola Adetula SiYuan Liu Min Zhu YaLan Yang XinHao Fan MuYa Chen YiJie Tang Yun Chen YuWen Liu GuoQiang Yi ZhongLin Tang Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenGuangdong 518124PR China Genome Analysis laboratory of the Ministry of Agriculture Agricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenGuangdong 518124PR China Institute of animal Science Chinese Academy of Agricultural SciencesBeijing 100193PR China

The microRNAs (miRNAs) play an important role in regulating myogenesis by targeting mRNA. However, the understanding of miRNAs in skeletal muscle development and diseases is unclear. In this study, we firstly performed the transcriptome profiling in differentiating C2C12 myoblast cells. Totally, we identified 187 miRNAs and 4260 mRNAs significantly differentially expressed that were involved in myoblast differentiation. We carried out validation of microarray data based on 5 mRNAs and 5 miRNAs differentially expressed and got a consistent result. Then we constructed and validated the significantly up- and down-regulated mRNA-miRNA interaction networks. Four interaction pairs (miR-145a-5p-Fscn1, miR-200c-5p-Tmigd1, miR-27a-5p-Sln and miR-743a-5p-Mob1b) with targeted relationships in differentiated myoblast cells were demonstrated. They are all closely related to myoblast development. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated cell cycle signals important for exploring skeletal muscle development and disease. Functionally, we discovered that miR-743a targeting gene Mps One Binder Kinase Activator-Like 1B (Mob1b) gene in differentiated C2C12. The up-regulated miR-743a can promote the differentiation of C2C12 myoblast. While the down-regulated Mob1b plays a negative role in differentiation. In addition, the expression profile of miR-743a and Mob1b are consistent with skeletal muscle recovery after Cardiotoxin (CTX) injury. Our study revealed that miR-743a-5p regulates myoblast differentiation by targeting Mob1b involved in skeletal muscle development and regeneration. Our findings made a further exploration for mechanisms in myogenesis and might provide potential possible miRNA-based target therapies for skeletal muscle regeneration and disease in the near future.

关键词： Differentiation miR 743a-5p Mob 1b Myoblast Skeletal muscle .Transcriptome

Experimental and computational study of annealed nickel sulfide quantum dots for catalytic and antibacterial activity

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Nano Materials Science 2024年第3期6卷 355-364页

作者： Muhammad Ikram Sawaira Moeen Ali Haider Anwar Ul-Hamid Haya Alhummiany Hamoud H.Somaily Souraya Goumri-Said Mohammed Benali Kanoun Solar Cell Applications Research Lab Department of PhysicsGovernment College University LahoreLahore54000PunjabPakistan Department of Clinical Sciences Faculty of Veterinary and Animal SciencesMuhammad Nawaz ShareefUniversity of Agriculture66000MultanPunjabPakistan Core Research Facilities King Fahd University of Petroleum&MineralsDhahran31261Saudi Arabia Department of Physics Faculty of ScienceUniversity of JeddahP.O.Box 13151Jeddah21493Saudi Arabia Department of Physics Faculty of ScienceKing Khalid UniversityP.O.Box 9004AbhaSaudi Arabia Physics Department College of Science and General StudiesAlfaisal UniversityP.O.Box 50927Riyadh11533Saudi Arabia Department of Mathematics and Sciences College of Humanities and SciencesPrince Sultan UniversityP.O.Box 66833Riyadh11586Saudi Arabia

This research investigates the hydrothermal synthesis and annealing duration effects on nickel sulfide(NiS_(2) quantum dots(QDs)for catalytic decolorization of methylene blue(MB)dye and antimicrobial *** size increased with longer annealing,reducing catalytic ***–vis,XRD,TEM,and FTIR analyses probed optical structural,morphological,and vibrational *** confirmed NiS2's anorthic structure,with crystallite size growing from 6.53 to 7.81 nm during extended ***–Vis exhibited a bathochromic shift,reflecting reduced band gap energy(Eg)in NiS_(2).TEM revealed NiS_(2)QD formation,with agglomerated QD average size increasing from 7.13 to 9.65 nm with prolonged *** NiS_(2) showed significant MB decolorization(89.85%)in acidic *** NiS_(2) QDs demonstrated notable antibacterial activity,yielding a 6.15mm inhibition zone against Escherichia coli(***)compared to ***-principles computation supported a robust interaction between MB and NiS_(2),evidenced by obtained adsorption *** study highlights the nuanced relationship between annealing duration,structural changes,and functional properties in NiS_(2)QDs,emphasizing their potential applications in catalysis and antibacterial interventions.

关键词： NiS_(2) antibacterial quantum dots dye degradation DFT

Evaluation of probiotic yoghurt by the mixed culture with Lactobacillus plantarum A3

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Food Science and human Wellness 2022年第2期11卷 323-331页

作者： Fengxuan Lang Jianzhu Wen Zhen Wu Daodong Pan Linjun Wang Key laboratory of animal Protein Deep Processing Technology of Zhejiang School of Food and Pharmaceutical SciencesNingbo UniversityNingbo 315211China School of Food Science&Pharmaceutical Engineering Nanjing Normal UniversityNanjing 210097China Qingyunshan Pharmaceutical GuangdongGuangzhou 512000China

Lactic acid bacteria(LAB)fermentation is the simplest and safest way of food preservation,and the use of probiotics in yoghurt could provide dairy products with unique flavors,textures and health *** this study,Lactobacillus bulgaricus,Streptococcus thermophilus,*** DSMZ 8533 and the potential probiotic strain *** A3 were used for the milk *** found the texture properties such as hardness,consistency,and viscosity of the yoghurt were enhanced in the mixed culture ***,components like amino acid(leucine),vanilla(vanillin),C;(unsaturated fatty acids)were also accumulated in *** A3 fermented yoghut,which leads to the significant sensory profiling difference compared with the former plain *** these results proved *** A3 is a potential probiotic stom winch could enhance the sensory and nutrition profiling of the fermented *** work still needs to be done on the synergistic interaction between the traditional strains and the probiotics during the fermentation process.

关键词： Lactobacillus plantarum Flavor profiles Volatile organic compounds Sensory profiles Fatty acid