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Effect of hepatocyte growth factor signaling pathway activation on Plasmodium berghei infection

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Asian Pacific Journal of Tropical Medicine 2010年第3期3卷 169-172页

作者： Nantian Zhong Fengying Huang Guanghong Tan Jiege Jiao Yingzhi Lin CaichunWang Hua Wang Songlin Zhou Yonghao Huang Fan Chen Yingying Lin Hainan Provincial Key laboratory of Tropical Medicine Hainan Medical University Department of histology and embryology Hainan Medical University

Objective:To explore the effect of hepatocyte growth factor signaling pathway activation on Plasmodium berghei ***:In this study,hepatocyte growth factor was detected by ELISA and Western blotting *** injury was detected by FITC-dextran absorption assay,and hepatocyte growth factor expression was shown to be expressed in the same injury cells by immunofluorescence against hepatocyte growth *** addition,Activation of hepatocyte growth factor and its receptor signaling pathway was detected with immunoprecipitation and detection of phosphorylation ***:It was found that injury of hepatocytes by sporozoite migration induced the secretion of hepatocyte growth factor and it was hepatocyte growth factor that rendered hepatocytes susceptible to Plasmodium sporozoite *** addition,hepatocyte infections depended on activation of the hepatocyte growth factor and its receptor signaling ***:Our results indicate that hepatocyte growth factor and its receptor may possibly be potential targets for new approaches to malaria treatment.

关键词： Hepatocyte growth factor Malaria Plasmodium sporozoite Signaling pathway

RELATION BETWEEN DISTRIBUTION OF ATRIOPEPTIN IMMUNOREACTIVE MYOCYTES AND STRUCTURAL CHANGES OF LEFT VENTRICULAR WALL IN PRENATAL AND POSTNATAL RATS

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Chinese Science Bulletin 1989年第7期34卷 615-616页

作者：纪中生李肇特 Department of histology and embryology Beijing Medical University Beijing

We have reported that parts of myocytes in ventricle of the rat embryos show atriopeptin immunoreactivity. In this letter the relationship between the structural evolution of left ventricular wall and the distribution... 详细信息

关键词： letter RELATION ventricle hematoxylin neonatal histology luminal remaining transformed sections

Proteomics of spermatogenesis： from protein lists to understanding the regulation of male fertility and infertility

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Asian Journal of Andrology 2011年第1期13卷 18-23页

作者： Xiaoyan Huang Jiahao Sha Lab of Reproductive Medicine Department of Histology and Embryology Nanjing Medical University Nanjing 210029 China

Proteomic technologies have undergone significant development in recent years, which has led to extensive advances in protein research. Currently, proteomic approaches have been applied to many scientific areas, including basic research, various disease and malignant tumour diagnostics, biomarker discovery and other therapeutic applications. In addition, proteomics-driven research articles examining reproductive biology and medicine are becoming increasingly common. The key challenge for this field is to move from lists of identified proteins to obtaining biological information regarding protein function. The present article reviews the available scientific literature related to spermatogenesis. In addition, this study uses two-dimensional electrophoresis mass spectrometry （2DE-MS） and liquid chromatography （LC）-MS to construct a series of proteome profiles describing spermatogenesis. This large-scale identification of proteins provides a rich resource for elucidating the mechanisms underlying male fertility and infertility.

关键词： infertility male fertility proteomics spermatogenesis

Immunohistochemical Study of EGF in Submandibular Gland During Healing of Gastric Ulcer in Rat

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Chinese Science Bulletin 1994年第11期39卷 960-964页

作者：赵培林李肇特 Department of histology and embryology Shanghai Medical UniversityShanghai 200032PRC. Department of histology and embryology Beijing Medical University Beijing 100083 PRC

The fact that organism can fully recover itself from certain visceral diseases sug-gests the existence of a natural disease-resistant mechanism, which mainly involvesneuro-endocrin regulation including the biological active peptides. Epidermalgrowth factor (EGF), which is a single-chain polypeptide consisting of 53 aminoacids, may be an important factor in this mechanism. EGF is synthesized

关键词： GCT cell EGF immunohistochemistry experimental gastric ulcer rat.

α-ketoglutarate promotes the specialization of primordial germ cell-like cells through regulating epigenetic reprogramming

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The Journal of Biomedical Research 2021年第1期35卷 36-46,I0014-I0016页

作者： Ming Xing Na Wang Hanyi Zeng Jun Zhang State Key laboratory of Reproductive Medicine Department of Histology and EmbryologyNanjing Medical UniversityNanjingJiangsu 211166China

There is growing evidence that cellular metabolism can directly participate in epigenetic dynamics and consequently modulate gene ***,the role of metabolites in activating the key gene regulatory network for specialization of germ cell lineage remains largely ***,we identified some cellular metabolites with significant changes by untargeted metabolomics between mouse epiblast-like cells(EpiLCs)and primordial germ cell-like cells(PGCLCs).More importantly,we found that inhibition of glutaminolysis by bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl)ethyl sulfide(BPTES)impeded PGCLC specialization,but the impediment could be rescued by addition ofα-ketoglutarate(αKG),the intermediate metabolite of oxidative phosphorylation and ***,adding aKG alone to the PGCLC medium accelerated the PGCLC specialization through promoting H3 K27 me3 ***,our study reveals the importance of metabolite aKG in the germ cell fate determination and highlights the essential role of cellular metabolism in shaping the cell identities through epigenetic events.

关键词： cellular metabolism α-ketoglutarate primordial germ cells epigenome

Role of stem cells in repair of liver injury:Experimental and clinical benefit of transferred stem cells on liver failure

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World Journal of Gastroenterology 2013年第40期19卷 6757-6773页

作者： Mukaddes Esrefoglu Department of histology and embryology Medical FacultyBezmialem Vakif University34093 IstanbulTurkey

Although the liver has a high regenerative capacity,as a result of massive hepatocyte death,liver failure occurs. In addition to liver failure,for acute,chronic and hereditary diseases of the liver,cell transplantation therapies can stimulate regeneration or at least ensure sufficient function until liver transplantation can be performed. The lack of donor organs and the risks of rejection have prompted extensive experimental and clinical research in the field of cellular transplantation. Transplantation of cell lineages involved in liver regeneration,including mature hepatocytes,fetal hepatocytes,fetal liver progenitor cells,fetal stem cells,hepatic progenitor cells,hepatic stem cells,mesenchymal stem cells,hematopoietic stem cells,and peripheral blood and umbilical cord blood stem cells,have been found to be beneficial in the treatment of liver *** this article,the results of experimental and clinical cell transplantation trials for liver failure are reviewed,with an emphasis on regeneration.

关键词： Liver regeneration Liver failure Stem cell

Generation of isogenic single and multiplex gene knockout mice by base editing-induced STOP

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Science Bulletin 2018年第17期63卷 1101-1107页

作者： Guang Yang Tianyu Zhu Zongyang Lu Guanglei Li Hao Zhang Songjie Feng Yajing Liu Jianan Li Yu Zhang Jia Chen Xuejiang Guo Xingxu Huang School of Life Science and Technology Shanghai Tech University Shanghai 201210 China State Key laboratory of Reproductive Medicine Department of Histology and Embryology Nanjing Medical University Nanjing 211166 China

Although CRISPR/Cas9 has been widely used to generate knockout mice, two major limitations remain:the founders usually carry a mixture of genotypes, and mosaicism harboring multiple ***, it takes a long time to get homozygous mutants. Recently developed base editing(BE) system,which introduces C-to-T conversion without double strand DNA cleavage, has been used to introduce artificial stop codons(i-STOP) to prematurely terminate translation, providing a cleaner strategy for genome engineering. Using this strategy, we generated CD160 KO and VISTA/CD160 double KO mice by microinjection of a single sg RNA targeting CD160 and a mixture of sg RNAs targeting VISTA and CD160,respectively. The BE system induced STOP efficiently in mouse embryos and consequently in founder mice without detectable off-target. Most interestingly, the majority of the mutants harbor same genetic modifications, indicating we generated isogenic single and multiplex gene mutant mice by BE-induced STOP. We also obtained homozygous mutant mouse in F1 mice, demonstrating the accelerated strategy in generating animal models.

关键词： Base editing i-STOP isogenic Knockout VISTA CDI60

Localization of somatostatin mRNA in early human placental villi by in situ hybridization histochemistry

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Chinese Science Bulletin 1996年第10期41卷 858-861页

作者：黄威权张荣庆唐美仪张崇理孙岚 Department of histology and embryology the Fourth Military Medical University Xi' an 710032 China

Previous immunohistochemical studies showed that the human placenta contained notonly many regulatory peptides, but also 5-HT and dopamine (DA) classicalneurotransmitters. These results made us suggest the following hypothesis: the human placentamight be a complex endocrine organ. In order to confirm this hypothesis, it must beunderstood whether these regulatory peptides and classical neurotransmitters can be pro-

关键词： somatostatin mRNA human placenta in situ hybridization.

Harnessing autophagy: A potential breakthrough in digestive disease treatment

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World Journal of Gastroenterology 2024年第24期30卷 3036-3043页

作者： Mukaddes Esrefoglu Department of histology and embryology Bezmialem Vakif University Medical FacultyIstanbul 34093Türkiye

Autophagy,a conserved cellular degradation process,is crucial for various cellular processes such as immune responses,inflammation,metabolic and oxidative stress adaptation,cell proliferation,development,and tissue repair and *** of autophagy is suspected in numerous diseases,including cancer,neurodegenerative diseases,digestive disorders,metabolic syndromes,and infectious and inflammatory *** autophagy is disrupted,for example,this can have serious consequences and lead to chronic inflammation and tissue damage,as occurs in diseases such as Chron's disease and ulcerative *** the other hand,the influence of autophagy on the development and progression of cancer is not *** can both suppress and promote the progression and metastasis of cancer at various *** inflammatory bowel diseases to gastrointestinal cancer,researchers are discovering the intricate role of autophagy in maintaining gut health and its potential as a therapeutic *** should carefully consider the nature and progression of diseases such as cancer when trying to determine whether inhibiting or stimulating autophagy is likely to be *** approaches that combine cutting-edge research with clinical expertise are key to unlocking the full therapeutic potential of autophagy in digestive diseases.

关键词： Autophagy Digestive disease Inflammatory bowel disease Gastrointestinal cancer Harnessing autophagy