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Expression profiling of the BpIAA gene family and the determination of IAA levels in Betula platyphylla tetraploids

Journal of forestry Research 2019年第3期30卷 855-867页

作者： Wendi Xu Su Chen Jing Jiang Guifeng Liu State Key laboratory of forest genetics and Tree breeding Northeast Forestry University

Betula platyphylla Sukaczev tetraploids have significantly larger leaf, fruit and stoma (gigantic phenotype) than diploids of the same species;however, the mechanism underlying this difference remains unclear. Tetraploid B. platyphylla transcriptome data have indicated that the expression of genes related to indole-3-acetic acid (IAA) biosynthesis and signal transduction was altered after genome duplication. IAA exerts pleiotropic effects on growth and development by inducing the expression of Aux/IAA. We identified 20 Aux/IAA genes (BpIAA1– BpIAA20) in B. platyphylla distributed across 10 chromosomes. Multiple alignment and motif analyses revealed that nine BpIAA proteins shared all four conserved domains. Phylogenetic analysis indicated that Aux/IAA families were divided into four subfamilies and that there were two pairs of BpIAA sister genes. The BpIAAs were differentially expressed in diploids and tetraploids. Moreover, the expression levels of the nine BpIAA genes were specifically up-regulated in tetraploids from June to September compared with May (except August 5th) in tetraploids, while they were down-regulated in diploids. IAA levels were more than twofold higher in tetraploids than diploids during the vegetative season. These results indicate that genome duplication of B. platyphylla caused the up-regulated of genes involved in IAA synthesis, and the increased concentration of IAA may induce the constitutive expression of 20 BpIAA genes. Therefore, the significant changes in the expression patterns of the BpIAAs contributed to the gigantic phenotype of tetraploids to some extent. Our research sheds light on the phenotypic variations observed in B. platyphylla tetraploids.

关键词： Betula platyphylla Suk. Tetraploid Aux/IAA Expression profile IAA

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Effects of a nitric oxide donor and nitric oxide scavengers on Sorbus pohuashanensis embryo germination

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Journal of forestry Research 2018年第3期29卷 628-635页

作者： Ling Yang Dongyan Zhang Hongnan Liu Cheng Wei Jianan Wang Hailong Shen State Key laboratory of forest genetics and breeding School of Forestry Northeast Forestry University

The effects of an exogenous nitric oxide donor(sodium nitroprusside, SNP), a NO scavenger 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxode(PTIO) and carboxy-PTIO potassium salt(c PTIO) on the embryo germination of Sorbus pohuashanensis were studied in a petri dish test. SNP at 0.5–5 mmol L-1 increased germination percentage, mean time to germination, germination index and germination energy compared with the control to different degrees. Treatment with 2 mmol L-1 SNP improved germination most significantly; embryo germination percentage for mother tree 1(91.11%) and mother tree 2(64.44%) were much higher than the control. In addition,excessive SNP levels did not enhance embryo *** treatment with SNP and an NO scavenger delayed embryo germination. Treatment with c PTIO inhibited embryo germination; germination percentage was 42.22% and was lower than that of the control. These results show that low concentrations of exogenous NO can enhance the embryo germination of S. pohuashanensis,providing a simple, effective way for promoting germination of S. pohuashanensis.

关键词： Mountain ash Embryo germination Nitric oxide Sodium nitroprusside Nitric oxide scavenger

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Growth response of Pterocarpus santalinus seedlings to native microbial symbionts(arbuscular mycorrhizal fungi and Rhizobium aegyptiacum)under nursery conditions

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Journal of forestry Research 2021年第1期32卷 225-231页

作者： Arumugam Karthikeyan Thangavel Arunprasad Institute of forest genetics and Tree breeding Coimbatore 641002India

The objective of this research was to improve the growth and biomass of Pterocarpus santalinus L.f.(an endangered leguminous tree)using native microbial symbionts such as arbuscular mycorrhizal fungi and Rhizobium associated with native populations of *** native arbuscular mycorrhizal fungi isolated from *** soils were identifi ed as(1)Glomus fasciculatum;(2)Glomus geosporum;and Glomus aggregatum.A nitrogenfi xing microbial symbiont was isolated from the root nodules of *** and identifi ed as Rhizobium aegyptiacum by 16s rRNA gene *** microbial symbionts were inoculated individually and in combination into *** seedling *** 90 days,growth and biomass had improved compared with uninoculated *** and root lengths,number of leaves,stem circumference,number of root nodules,biomass,nutrient uptake and seedling quality index were signifi cantly increased by a combined inoculation of arbuscular mycorrhizal fungi+Rhizobium *** was concluded that native microbial symbionts positively infl uenced *** seedling growth which will be helpful for successful fi eld establishment.

关键词： Arbuscular mycorrhizal fungi Microbial symbionts Pterocarpus santalinus Red sanders Rhizobium aegyptiacum

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Responses of Bruguiera sexangula propagules to beneficial microbes in the nursery

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Journal of forestry Research 2018年第4期29卷 1082-1087页

作者： Arumugam Karthikeyan Natchimuthu Balasubramaniam Sivapriya Institute of forest genetics and Tree breeding Coimbatore 641002 India

Bruguiera sexangula （Lout） Poir., a threatened mangrove tree, was inoculated with beneficial microbes in a nursery to assess any improvements in growth and bio- mass. From soil samples from the rhizosphere of B. sexangula in a mangrove forest in Panangadu of Kerala India, nitrogen-fixing bacteria Azotobacter chroococcum and Azospirillum brasilense were isolated. The phosphatesolubilising bacterium Bacillus megaterium and potassiummobilizing bacteria Frateruria aurantia were also isolated and cultured on suitable media. Later, ripe propagules of B. sexangula were collected from matured trees and raised in sterilized soil bags （13 × 25 cm） containing sterilized soil and sand （2：1 ratio）. The cultured beneficial microbes were propagated and used to inoculate the ripe propagules of B. sexangula and maintained in the nursery for 6 months. After 6 months, growth and biomass of the inoculated propagules were greater than for the uninoculated control propagules. Shoot length, number of leaves, stem girth and root length were also significantly greater than in the controls. This study showed that the mangrove-specific beneficial microbes influenced the growth of B. sexangula

关键词： Mangroves Bruguiera sexangula Beneficial microbes Nursery Propagules

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Comparison of methods for extracting high-throughput sequencing RNA from Korean pine seeds

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Journal of forestry Research 2016年第1期27卷 33-40页

作者： Yan Liang Hai-long Shen Chun-ping Liu Ling Yang Peng Zhang State Key laboratory of forest genetics and breeding Northeast Forestry University School of Life Sciences and A & F Qiqihar University

Korean pine （Pinus koraiensis Sieb. et Zucc.） is an ecologically and economically important tree species in East Asia. Molecular studies of seed development of this species are limited due to the lack of effective RNA extraction protocols. This study aimed to obtain an effec- tive method to extract high-quality RNA from Korean pine seeds. The TRIzol kit and CTAB methods were used to extract the total RNA from Korean pine seeds at different developmental stages. The bands of RNA extracted by CTAB were not clear, whereas the bands of RNA extracted by the TRIzol kit were brighter and clearer, indicating higher quality and integrity of the RNA products extracted by the TRIzol kit. The 28S rRNA band was approximately 1.5- to 2-fold brighter than the 18S rRNA band on the agarose gel electrophoresis. The absorbance value A 260/280 was 1.8-2.0, and the absorbance value A 260/230 was 〉1.9. The Bioanalyzer RNA integrity results showed that the RNA integrity number of the RNA extracted using the TR/zol kit was acceptable for high-throughputsequencing. Therefore, the total RNA extracted using the TRIzol kit method can be used for high-throughput sequencing and other molecular biology experiments.

关键词： Pinus koraiensis Seeds RNA extraction CTAB TRIzol kit

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Identification and Characterization of the Populus AREB/ABF Subfamily

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Journal of Integrative Plant Biology 2013年第2期55卷 177-186页

作者： Lexiang Ji Jia Wang Meixia Ye Ying Li Bin Guo Zhong Chen Hao Li Xinmin An National Engineering laboratory for Tree breeding NDRC Key laboratory of genetics and breeding in forest Trees and Ornamental Plants MOE The Tree and Ornamental Plant breeding and Biotechnology laboratory SFA College of Biological Sciences and Biotechnology Beijing Forestry University

Abscisic acid （ABA） is a major plant hormone that plays an important role in responses to abiotic stresses. The ABA-responsive element binding protein/ABRE-binding factor （AREB/ABF） gene subfamily contains crucial transcription factors in the ABA-mediated signaling pathway. In this study, a total of 14 putative AREB/ABF members were identified in the Populus trichocarpa Torr. ＆ Gray. genome using five AREB/ABF amino acid sequences from Arabidopsis thaliana L. as probes. The 14 putative Populus subfamily members showed high protein similarities, especially in the basic leucine zipper （bZIP） domain region. A neighbor-joining analysis combined with gene structure data revealed homology among the 14 genes. The expression patterns of the Populus AREB/ABF subfamily suggested that the most abundant transcripts of 11 genes occurred in leaf tissues, while two genes were most transcribed in root tissues. Significantly, eight Populus AREB/ABF gene members were upregulated after treatment with 100 t^M exogenous ABA, while the other six members were downregulated. We identified the expression profiles of the subfamily members in Populus tissues and elucidated different response patterns of Populus AREB/ABF members to ABA stress. This study provided insight into the roles of Populus AREB/ABF homologues in plant response to abiotic stresses.

关键词： Arabidopsis thafiana Populus AREB/ABF bioinformatics qRT-PCR.

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The liver transcriptome of two full-sibling Songliao black pigs with extreme differences in backfat thickness

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Journal of Animal Science and Biotechnology 2015年第1期6卷 13-21页

作者： Kai Xing Feng Zhu Liwei Zhai Huijie Liu Zhijun Wang Zhuocheng Hou Chuduan Wang National Engineering laboratory for Animal breeding and MOA Key laboratory of Animal genetics and breeding Department of Animal Genetics and BreedingChina Agricultural University Tianjin Ninghe Primary Pig breeding Farm

Background： Fatness traits in animals are important for their growth, meat quality, reproductive performance, and immunity. The liver is the principal organ of the regulation of lipid metabolism, and this study used massive parallelized high-throughput sequencing technologies to determine the porcine liver tissue transcriptome architecture of two full-sibling Songliao black pigs harboring extremely different phenotypes of backfat thickness. Results： The total number of reads produced for each sample was in the region of 53 million, and 8,226 novel transcripts were detected. Approximately 92 genes were differentially regulated in the liver tissue, while 31 spliced transcripts and 33 primary transcripts showed significantly differential expression between pigs with higher and lower backfat thickness. Genes that were differentially expressed were involved in the metabolism of various substances, smal molecule biochemistry, and molecular transport. Conclusions： Genes involved in the regulation of iipids could play an important role in lipid and fatty acid metabolism in the liver. These results could help us understand how liver metabolism affects the backfat thickness of pigs.

关键词： Backfat thickness Liver Pig RNA-Seq

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Genetic structure of needle morphological and anatomical traits of Pinus yunnanensis

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Journal of forestry Research 2016年第1期27卷 13-25页

作者： Yujie Huang Jianfeng Mao Zhiqiang Chen Jingxiang Meng Yulan Xu Anan Duan Yue Li National Engineering laboratory for forest Tree breeding Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants of Ministry of Education College of Biological Sciences and Technology Beijing Forestry University Key laboratory for forest Resources Conservation and the Southwest Mountains of China Ministry of Education Southwest Forestry University

Pinus yunnanensis Franch. is an particular conifer tree species in Yunnan-Guizhou plateau in south- west China. The morphological and anatomical traits of needles are important to evaluate geographic variation and population dynamics of conifer species. Seedlings from seven populations of P. yunnanensis were analyzed, look- ing at 22 morphological and anatomical needle traits. The results showed that variations among and within popula- tions were significantly different for all traits and the variance components within populations were generally higher than that among populations in the most tested needle traits. The proportions of three-needle fascicle were significantly different among populations. The traits related to needle size in both morphology and anatomy were positive with latitude and negative with annual temperature and precipitation. Ratio indices, including mesophyllarea/vascular bundle area, mesophyll area/resin canals area, vascular bundle area/resin canals area and mesophyU area/（resin canals area and vascular bundle area）, were negatively correlated with elevation and positively corre- lated with the annual mean temperature, showing some fitness feature for the populations. Needle traits were more significantly correlated with longitude than with other four environmental factors. Needle length was significantly correlated with almost all environmental factors. First four principal components accounted for 81.596 % of the variation with eigenvalues 〉1; the differences among populations were mainly dependent on needle width, stomatal density, section areas of vascular bundle, total resin canals, and mesophyll, as well as area ratio traits. Seven populations were divided into three categories by Euclidean distance. Variations in needle traits among the populations have shown systematic microevolution in terms of geographic impact on P. yunnanensis. This study would provide empirical data to characterize adaptation and genetic variation of P. yunnanens

关键词： Genetic structure Needle Morphologicaland anatomical traits Geographic population Pinusyunnanensis

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Optimizing Seed Water Content:Relevance to Storage Stability and Molecular Mobility

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Journal of Integrative Plant Biology 2010年第3期52卷 324-331页

作者： Ming Zhang Jia-Jin Zhuo Xu Wang Sen Wu Xiao-Feng Wang Key laboratory for genetics and breeding in forest Trees and Ornamental Plants Ministry of Education Beijing Forestry University Beijing 100083 China

This research was conducted to determine the optimum moisture content （MC） that gave maximum longevity to seeds. Three species were used to represent seeds with different dry matter reserves, which gives them different sorption properties： maize （Zea mays L.）, elm （Ulmus pumila L.） and safflower （Carthamus tinctorius L.）. The seeds of elm, safflower, and maize embryos with MC ranging from 0.00- 0.15 g H2O/g dry weight （DW） were stored at 35℃ for different periods of time. The results showed that the optimum MC for seed and embryo storage varied between species （0.057 g H20/g DW for maize embryos, 0.045 g H20/g DW for elm, and 0.02 g H2O/g DW for safflower）. Drying below this optimum MC increased the aging rate and there were detrimental effects of drying. The relative humidity corresponding to optimum MC in embryos of maize, elms and safflower was about 15%, 12% and 7% respectively, according to the lipid composition of the embryos. The data provided confirmatory evidence that molecular mobility （△Azz） in elms, maize and safflower embryos was compatible with the optimum moisture content.

关键词： SEEDS MOISTURE CORN ELM SAFFLOWER MOLECULAR microbiology QUANTITATIVE research

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Progress in the Study of Molecular Genetic Improvements of Poplar in China

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Journal of Integrative Plant Biology 2006年第9期48卷 1001-1007页

作者： Shan-Zhi Lin Zhi-Yi Zhang Qian Zhang Yuan-Zhen Lin Key laboratory for genetics and breeding in forest Trees and Ornamental Plants of Ministry of Education Beijing forestry University Beijing 100083 China

The poplar is one of the most economically important and intensively studied tree species owing to its wide application in the timber industry and as a model material for the study of woody plants. The natural resource of poplars in China is replete. Over the past 10 years, the application of molecular biological techniques to genetic improvements in poplar species has been widely studied in China. Recent advances in molecular genetic improvements of poplar, including cDNA library construction, gene cloning and identification, genetic engineering, gene expression, genetic linkage map construction, mapping of quantitative trait loci （QTL） and molecular-assisted selection, are reviewed in the present paper. In addition, the application of modern biotechnology to molecular improvements in the genetic traits of the poplar and some unsolved problems are discussed.

关键词： poplar gene engineering genetic improvement genetic linkage maps molecular marker quantitative trait loci (QTL) mapping

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