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Rapid and specific fluorescent probe visualizes dynamic correlation of Cys and HClO in OGD/R

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Chinese Chemical Letters 2025年第1期36卷 437-441页

作者： Pei Huang Weijie Zhang Junping Wang Fangjun Huo Caixia Yin Institute of molecular Science Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of EducationShanxi UniversityTaiyuan 030006China Research Institute of Applied Chemistry Shanxi UniversityTaiyuan 030006China

Intracellular redox homeostasis is of indispensable importance in *** order to maintain the balance of the redox state within the cell,reactive oxygen species(ROS)and reactive sulfur species(RSS)react and transform with each other,and their levels also directly reflect the degree of oxidative stress and *** acid(HClO)and cysteine(Cys)usually co-exist in organisms,interacting with each other in many important physiological processes and synergistically maintaining the dynamic redox balance in the *** understand the relevance and pathophysiological effects of these two signaling molecules in oxidative stress,unique fluorescence imaging tools are ***,we designed and developed a dual-channel fluorescent probe HP,for the individual and continuous detection of HClO and *** probe could simultaneously monitor the changes in the concentrations of HClO and Cys in cells,and was characterized by a fast response,high sensitivity and high selectivity,especially compared with glutathione(GSH)and homocysteine(Hcy),the probe had a good specificity for ***,probe HP successfully observed dynamic changes in HCl O-and Cys-mediated redox status in the oxygenglucose deprivation/reperfusion(OGD/R)model of HeLa cells and dynamically monitored fluctuations in endogenous HClO levels in lipopolysaccharides(LPS)-induced peritonitis mice.

关键词： Redox homeostasis Dual-channel OGD/R Fluctuations Peritonitis

Development of ESIPT-based specific fluorescent probes for bioactive species based on the protection-deprotection of the hydroxyl

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Chinese Chemical Letters 2025年第2期36卷 49-56页

作者： Haixian Ren Yuting Du Xiaojing Yang Fangjun Huo Le Zhang Caixia Yin Department of Chemistry Xinzhou Normal University Research Institute of Applied Chemistry Shanxi University Department of Chemistry The University of Texas at Austin Key laboratory of Chemical biology and molecular Engineering of Ministry of Education Key Laboratory of Materials for Energy Conversion and Storage of Shanxi Province Institute of Molecular ScienceShanxi University

Excited-state intramolecular proton-transfer (ESIPT) based fluorescence probes are particularly attractive due to their unique properties including environmental sensitivity, a large Stokes shift, and potential for ratiometric sensing. In general, ESIPT-based fluorophore incorporates an intramolecular hydrogen bonding interaction between a hydrogen bond donor (–OH and NH2are common) and a hydrogen bond acceptor(C=N and C=O). More, protection–deprotection of hydroxyl group as hydrogen bond donor could induce an off-on switch of ESIPT-based emission. Therefore, protection–deprotection of hydroxyl group has been the widely used strategy to design fluorescent probes, where the potential key issue is selecting a protective group that can specifically leave in the presence of the target analyte. In this review, we mainly summarize the specific protecting groups (sites) and deprotection mechanisms for biologically important species (including reactive sulfur species (RSS), reactive oxygen species (ROS), enzymes, etc.), and analyze the advantages and disadvantages of different protection mechanisms from some aspects including probe stability, selectivity, response rate and assay system, etc. Based on the aforementioned, we further point out the current challenges and the potential future direction for developing ESIPT-based probes.

关键词： Bioimaging ESIPT Fluorescence probe Protection-deprotection Specificity

TavWA1 is critical for wheat growth by modulating cell morphology and arrangement

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Journal of Integrative Plant biology 2025年第1期67卷 71-86页

作者： Guowei Chang Yue Li Lei Peng Chuncai Shen Yipeng Lu Wan Teng Yangyang Liu Yingchun Wang Weiqi Zhu Cuimin Liu Xue He Yiping Tong Xueqiang Zhao Key laboratory of Seed Innovation Institute of Genetics and Developmental BiologyThe Innovative Academy of Seed DesignChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Key laboratory of Plant molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China State Key laboratory of molecular Developmental biology Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Zhaoxian Experiment Station Shijiazhuang Academy of Agricultural and Forestry SciencesZhaoxian 515300China

Plant growth is determined by the production of cells and initiation of new *** genes that control cell number and cell size is of great significance for understanding plant growth *** this study,we characterized two wheat mutants,ah and dl,with abnormal *** ah mutant is a naturally occurring variant characterized by severe dwarfism,increased tiller number,and reduced grain length,while the dl mutant is derived from an ethyl methane sulfonate(EMS)-mutagenized population and exhibits smaller grain size and slightly reduced plant *** analyses revealed abnormal cell number,cell morphology and arrangement in the stems and leaves of the ah mutant,along with reduced cell length in the grains of the dl ***-based cloning identified that both mutants carry mutations in the same gene TavWA1-7D,which encodes a protein with a von Willebrand factor A(vWA)*** ah mutant harbors a 174-bp insertion in the 1,402-bp coding sequence(CDS)of TavWA1-7D,causing premature termination of protein translation,while the dl mutant contains a Glu420Lys *** the TavWA1-7D ahthrough clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated nuclease 9-mediated genome editing leads to a severe dwarfism *** C-terminus of the protein is crucial for its correct subcellular localization and interaction,supporting its critical role for TavWA1-7D *** analysis showed that the dwarf phenotype of the ah mutant is associated with impaired photosynthesis,ribosome function,and nucleosome ***,TavWA1-7D interacts with an E3 ligase,Ta VIP1-3B,the expression levels of which are elevated in both *** and knockout studies of TaVIP1-3B demonstrated its negative regulatory role in cell length and grain ***,our findings suggest that TavWA1-7D plays a vital role in regulating wheat growth and yield-related traits,with the dl mutant's short grain phen

关键词： grain size severe dwarf TaVIP1 TavWA1 wheat

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Efficient generation of all ESC-derived mice carrying a homozygous lethal mutation through eight-cell embryo injection

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Science China Life Sciences 2025年

作者： Xidi Yin Meng Yan Yanbo Cheng Zhoujie Li Chong Cui Yingyi Wang Nan Liu Yilai Shu Jinsong Li Qing Li Key laboratory of Multi-cell Systems Shanghai Key Laboratory of Molecular Andrology Shanghai Institute of Biochemistry and Cell Biology Center for Excellence in Molecular Cell Science Chinese Academy of Sciences School of Life Science and Technology ShanghaiTech University Animal Core Facility Shanghai Institute of Biochemistry and Cell Biology Center for Excellence in Molecular Cell Science Chinese Academy of Sciences ENT Institute and Department of Otorhinolaryngology Eye & ENT Hospital State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Fudan University Key laboratory of Systems and Health Science of Zhejiang Province School of Life Science Hangzhou Institute for Advanced Study University of Chinese Academy of Sciences

Dear Editor,Large-scale phenotyping efforts have shown that placental defects correlate strongly with embryonic lethal mouse mutants(Perez-Garcia et al., 2018). To rescue normal development of these mutant embryos, tetraploid complementation can be a reliable strategy, in which,tetraploid cells form extraembryonic lineages thus providing the embryo with a wild-type placenta(Nagy et al., 1990).

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Angiotensin-converting enzyme 2 and hepatic SARS-CoV-2 infection:Regulation,association,and therapeutic implications

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World Journal of Gastroenterology 2025年第6期31卷 8-13页

作者： Yu-Wei Luo Ai-Long Huang Kai-Fu Tang Key laboratory of molecular biology on Infectious Disease Ministry of EducationChongqing Medical UniversityChongqing 400016China

Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)enters host cells via the angiotensin-converting enzyme 2(ACE2)*** evidence has indicated the presence of hepatic SARS-CoV-2 infection and liver injury in pa-tients with coronavirus disease 2019(COVID-19).Understanding the mechanisms of hepatic SARS-CoV-2 infection is crucial for addressing COVID-19–related liver pathology and developing targeted *** editorial discusses the signi-ficance of ACE2 in hepatic SARS-CoV-2 infection,drawing on the research by Jacobs et *** findings indicate that hepatic ACE2 expression,frequency of hepatic SARS-CoV-2 infection,and severity of liver injury are elevated in patients with pre-existing chronic liver *** data suggest that hepatic ACE2 could be a promising therapeutic target for COVID-19.

关键词： Hepatic angiotensin-converting enzyme 2 SARS-CoV-2 Liver infection Chronic liver diseases COVID-19 treatment

Fibrotic liver extracellular matrix induces cancerous phenotype in biomimetic micro-tissues of hepatocellular carcinoma model

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Hepatobiliary & Pancreatic Diseases International 2025年第1期24卷 92-103页

作者： Kosar Nouri Abbas Piryaei Homeyra Seydi Ibrahim Zarkesh Ibrahim Ghoytasi Bahare Shokouhian Mustapha Najimi Massoud Vosough Department of Developmental biology University of Science and CultureACECR 14155-4364 TehranIran Department of Regenerative Medicine Cell Science Research CenterRoyan Institute for Stem Cell Biology and TechnologyACECR 14155-4364 TehranIran Department of Stem cells and Developmental biology Cell Science Research CenterRoyan Institute for Stem Cell Biology and TechnologyACECR 14155-4364 TehranIran Department of biology and Anatomical Sciences School of MedicineShahid Beheshti University of Medical SciencesTehranIran Department of Tissue Engineering and Applied cell Sciences School of Advanced Technologies in MedicineShahid Beheshti University of Medical SciencesTehranIran Department of cell Engineering Cell Science Research CenterRoyan Institute for Stem Cell Biology and TechnologyACECR 14155-4364 TehranIran Department of Medical Biotechnology School of Advanced Technologies in MedicineTehran University of Medical SciencesTehranIran laboratory of Pediatric Hepatology and cell Therapy Institute of Experimental and Clinical Research(IREC)UCLouvainBrusselsBelgium Experimental Cancer Medicine Institution for Laboratory MedicineKarolinska InstituteStockholmSweden

Background: Despite considerable advancements in identifying factors contributing to the development of hepatocellular carcinoma(HCC), the pathogenesis of HCC remains unclear. In many cases, HCC is a consequence of prolonged liver fibrosis, resulting in the formation of an intricate premalignant microenvironment. The accumulation of extracellular matrix(ECM) is a hallmark of premalignant microenvironment. Given the critical role of different matrix components in regulating cell phenotype and function, this study aimed to elucidate the interplay between the fibrotic matrix and malignant features in HCC. Methods: Liver tissues from both control(normal) and carbon tetrachloride(CCl_(4))-induced fibrotic rats were decellularized using sodium dodecyl sulfate(SDS) and Triton X-100. The resulting hydrogel from decellularized ECM was processed into micro-particles via the water-in-oil emulsion method. Microparticles were subsequently incorporated into three-dimensional liver biomimetic micro-tissues(MTs) comprising Huh-7 cells, human umbilical vein endothelial cells(HUVECs), and LX-2 cells. The MTs were evaluated using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium(MTS) assay at day 11, immunofluorescence staining, immunoblotting, and spheroid migration assay at day 14 after co-culture. Results: Fibrotic matrix from CCl4-treated rat livers significantly enhanced the growth rate of the MTs and their expression of CCND1 as compared to the normal one. Fibrotic matrix, also induced the expression of epithelial-to-mesenchymal transition(EMT)-associated genes such as TWIST1, ACTA2, MMP9, CDH2, and VIMENTIN in the MTs as compared to the normal matrix. Conversely, the expression of CDH1 and hepatic maturation genes HNF4A, ALB, CYP3A4 was decreased in the MTs when the fibrotic matrix was used. Furthermore, the fibrotic matrix increased the migration of the MTs and their secretion of alpha-fetoprotein. Conclusions: Our findings suggest a

关键词： Liver fibrosis Decellularized extracellular matrix Spheroid Epithelial-to-mesenchymal transition cell differentiation Hepatocellular carcinoma

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Eukaryotic translation initiation factor OseIF5A4 negatively regulates rice immunity against blast and bacterial blight diseases

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The Crop Journal 2025年第1期13卷 79-91页

作者： Hui Wang Yuqing Yan Yan Bi Dayong Li Fengming Song National Key laboratory for Rice biology and Breeding Institute of BiotechnologyZhejiang UniversityHangzhou 310058ZhejiangChina Ministry of Agriculture Key laboratory of molecular biology of Crop Pathogens and Insect Pests Institute of BiotechnologyZhejiang UniversityHangzhou 310058ZhejiangChina Key laboratory of biology of Crop Pathogens and Insects of Zhejiang Province Institute of BiotechnologyZhejiang UniversityHangzhou 310058ZhejiangChina

The RING-type E3 ligase OsBBI1 regulates rice resistance against Magnaporthe oryzae through modifying cell wall *** this study,we report the function of an OsBBI1 substrate,eukaryotic translation initiation factor OseIF5A4,in rice ***1 interacts with OseIF5A4 and other four members of the OseIF5A *** RING domain in OsBBI1 and the eIF-5a domain in OseIF5A4 are critical for the OsBBI1-OseIF5A4 ***1 ubiquitinates OseIF5A4 and mediates its degradation in vitro and in ***,the expression of OseIF5A4 was upregulated during early stage of compatible interaction but downregulated in incompatible interaction between rice and *** of OseIF5A4 enhances rice immunity against *** and Xanthomonas oryzae ***,boosts pattern-triggered immune responses,and strengthens pathogen-induced defense responses(e.g.,expression of defense genes,accumulation of reactive oxygen species and reinforcement of cell wall).However,overexpression of OseIF5A4 attenuates rice immunity and immune *** results demonstrate that OseIF5A4,a substrate of the immunity-associated E3 ligase OsBBI1,negatively regulates rice immunity against *** and *** *** through modulating pathogen-induced defense responses,highlighting the importance of the protein translational machinery in rice immunity.

关键词： Magnaporthe oryzae OsBBI1 OseIF5A Rice immunity Xanthomonas oryzae pv.oryzae

KDM2A and KDM2B protect a subset of CpG islands from DNA methylation

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Journal of Genetics and Genomics 2025年第1期52卷 39-50页

作者： Yuan Liu Ying Liu Yunji Zhu Di Hu Hu Nie Yali Xie Rongrong Sun Jin He Honglian Zhang Falong Lu State Key laboratory of molecular Developmental biology Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Department of Biochemistry&molecular biology College of Natural ScienceMichigan State UniversityEast LansingMI 48824US State Key laboratory of Animal Nutrition and Feeding Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193China

In the mammalian genome,most CpGs are ***,CpGs within the CpG islands(CGIs)are largely unmethylated,which are important for gene expression *** mechanism underlying the low methylation levels at CGIs remains largely ***2 proteins(KDM2A and KDM2B)are H3K36me2 demethylases known to bind specifically at ***,we report that depletion of each or both KDM2 proteins,or mutation of all their JmjC domains that harbor the H3K36me2 demethylation activity,leads to an increase in DNA methylation at selective *** Kdm2a/2b double knockout shows a stronger increase in DNA methylation compared with the single mutant of Kdm2a or Kdm2b,indicating that KDM2A and KDM2B redundantly regulate DNA methylation at *** addition,the increase of CGI DNA methylation upon mutations of KDM2 proteins is associated with the chromatin *** findings reveal that KDM2A and KDM2B function redundantly in regulating DNA methylation at a subset of CGIs in an H3K36me2 demethylation-dependent manner.

关键词： KDM2A KDM2B CpG island DNA methylation H3K36me2 Demethylation Embryonic stemcell

Neuroinflammation revisited through the microglial lens

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Neural Regeneration Research 2025年第7期20卷 1989-1990页

作者： Renato Socodato João B.Relvas Institute of Research and Innovation in Health(i3S)and Institute for molecular and cell biology(IBMC) University of PortoPortoPortugal Department of Biomedicine Faculty of Medicine of the University of Porto(FMUP)PortoPortugal

Neuroimmunology is emerging as a pivotal field,shedding light on the intricate dialogues between the central nervous system(CNS)and the immune *** exploration is particularly significant in understanding microglia,the CNS’s innate immune cells,beyond the conventional conflation of“neuroinflammation”and“microglial activation.”This conflation has clouded the true complexity of these processes,potentially stalling scientific progress and the development of new *** challenge the long-standing perspectives that have oversimplified these interactions,advocating for a deeper exploration of the dynamic relationship between neuroinflammation and microglial *** dissecting specific molecular pathways,we aim to illuminate their elaborate roles in neuroinflammatory responses,especially in the context of Alzheimer’s disease(AD).Here,neuroinflammation is not merely a passive observer or a direct antagonist but a complex agent in the disease’s *** article calls for a significant paradigm shift towards an integrative,multi-omics approach to *** such a comprehensive framework is crucial for advancing our understanding of neuroinflammatory conditions and paving the way for targeted therapeutic strategies for brain diseases.

关键词： inflammation simplified activation

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