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Molecular Plant 2024年第8期17卷 1289-1306页

作者： Wei Zhang Shufei Tang Xuying Li Yuanyuan Chen Jiajia Li Yuyang Wang Ruichao Bian Ying Jin Xiaoxian Zhu Kewei Zhang Zhejiang Provincial Key laboratory of biotechnology on Specialty Economic Plants College of Life SciencesZhejiang Normal UniversityJinhuaZhejiang 321004P.R.China

Monocarpic senescence,characterized by whole-plant senescence following a single flowering phase,is widespread in seed plants,particularly in crops,determining seed harvest time and ***,how external and internal signals are systemically integrated into monocarpic senescence remains largely ***,we report that the Arabidopsis thaliana transcription factor WRKY1 plays essential roles in multiple key steps of monocarpic ***1 expression is induced by age,salicylic acid(SA),and nitrogen(N)*** and leaf senescence are accelerated in the WRKY1 overexpression lines but are delayed in the wrky1 *** combined DNA affinity purification sequencing and RNA sequencing analyses uncover the direct target genes of *** studies show that WRKY1 coordinately regulates three processes in monocarpic senescence:(1)suppressing FLOWERING LOCUS C gene expression to initiate flowering,(2)inducing SA biosynthesis genes to promote leaf senescence,and(3)activating the N assimilation and transport genes to trigger N *** summary,our study reveals how one stress-responsive transcription factor,WRKY1,integrates flowering,leaf senescence,and N remobilization processes into monocarpic senescence,providing important insights into plant lifetime regulation.

关键词： monocarpic senescence leaf senescence flowering time nitrogen remobilization salicylic acid WRKY transcription factor

Tbx 4 and Tbx 5 gene expression associated with appendage development and its relationship with the absence of the pelvic fin in Pampus argenteus

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Journal of Oceanology and Limnology 2024年第2期42卷 580-593页

作者： Shun ZHANG Xiaojing ZHU Lingzhu HU Kai LIAO Shanliang XU Danli WANG Chunyang GUO School of Marine Science Ningbo UniversityNingbo 315211China Key laboratory of Applied Marine biotechnology Ningbo UniversityChinese Ministry of EducationNingbo 315211China

The Tbx family is first known through the study of their functions in the body and limbs,and its members Tbx4 and Tbx5 genes are important factors in determining the characteristics of the *** argenteus is one of the important economical marine fishes widely distributed in offshore ***,it is necessary to study the role of Tbx family genes in the deletion of pelvic fin in *** this study,we cloned Tbx4 and Tbx5 cDNA sequence of ***(GenBank:MH709128 and MH712458).The Western blot and real time PCR were used to detect the expressions of Tbx4 and Tbx5 in different developmental stages and tissues of *** addition,whole-mount in-situ hybridization was used to study the localization of Tbx4 and Tbx5 genes in different developmental stages of *** show that the translation of Tbx4 mRNA was inhibited during the critical period of pelvic fin *** different tissues,Tbx4 protein levels were the lowest in the abdominal epithelium,and even lower than that in the pectoral fin,suggesting that the protein expression was also inhibited in the abdominal epithelium of adult ***,the results indicated that upstream genes regulation led to the key stage-specific and low expression of Tbx4 during pelvic fin development and in the abdominal epithelium.

关键词： Stromateidae pelvic fin protein translation T-box aquaculture broodstock

Population genomic data reveal low genetic diversity,divergence and local adaptation among threatened Reeves's Pheasant(Syrmaticus reevesii)

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Avian Research 2024年第1期15卷 1-11页

作者： Qi Lu Pengcheng Wang Jiang Chang De Chen Shenghan Gao Jacob Hoglund Zhengwang Zhang Ministry of Education Key laboratory for Biodiversity Science and Ecological Engineering College of Life SciencesBeijing Normal UniversityBeijing100875China Jiangsu Key laboratory for Biodiversity and biotechnology College of Life SciencesNanjing Normal UniversityNanjing210023China State Key laboratory of Environmental Criteria and Risk Assessment Chinese Research Academy of Environmental SciencesBeijing100012China State Key laboratory of Microbial resources Institute of MicrobiologyChinese Academy of SciencesBeijing100101China Department of Ecology and Genetics Animal EcologyUppsala UniversityUppsalaSweden

Population genomic data could provide valuable information for conservation efforts;however,limited studies have been conducted to investigate the genetic status of threatened ***’s Pheasant(Syrmaticus reevesii)is facing population decline,attributed to increases in habitat *** is a knowledge gap in understanding the genomic status and genetic basis underlying the local adaptation of this threatened ***,we used population genomic data to assess population structure,genetic diversity,inbreeding patterns,and genetic ***,we identified candidate genes linked with adaptation across the current distribution of Reeves’s *** present study assembled the first de novo genome sequence of Reeves’s Pheasant and annotated 19,458 *** also sequenced 30 individuals from three populations(Dabie Mountain,Shennongjia,Qinling Mountain)and found that there was clear population structure among those *** comparing with other threatened species,we found that Reeves’s Pheasants have low genetic *** of homozygosity suggest that the Shennongjia population has experienced serious *** demographic history results indicated that three populations experienced several declines during the glacial *** adaptative analysis among the populations identified 241 candidate genes under directional *** are involved in a large variety of processes,including the immune response and *** results suggest that the three populations should be considered as three different conservation *** current study provides genetic evidence for conserving the threatened Reeves’s Pheasant and provides genomic resources for global biodiversity management.

关键词： Conservation genetics Local adaptation Pheasant Whole-genome sequencing

m^(6)A modification of plant virus enables host recognition by NMD factors in plants

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Science China(Life Sciences) 2024年第1期67卷 161-174页

作者： Hao He Linhao Ge Yalin Chen Siwen Zhao Zhaolei Li Xueping Zhou Fangfang Li State Key laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China State Key laboratory of Rice Biology Institute of BiotechnologyZhejiang UniversityHangzhou 310029China

N^(6)-methyladenosine(m^(6)A)is the most abundant eukaryotic mRNA modification and is involved in various biological *** evidence has implicated that m^(6)Amodification is an important anti-viral defense mechanism in mammals and plants,but it is largely unknown how m^(6)Aregulates viral infection in *** we report the dynamic changes and functional anatomy of m^(6)Ain Nicotiana benthamiana and Solanum lycopersicum during Pepino mosaic virus(PepMV)infection.m^(6)Amodification in the PepMV RNA genome is conserved in these two *** of the m^(6)Awriters,mRNA adenosine methylase A(MTA),and HAKAI inhibit the PepMV RNA accumulation accompanied by increased viral m^(6)Amodifications,whereas deficiency of these writers decreases the viral RNA m^(6)Alevels but enhances virus *** study reveals that the cytoplasmic YTH-domain family protein NbECT2A/2B/2C as m^(6)Areaders are involved in anti-viral ***-protein interactions indicate that NbECT2A/2B/2C interact with nonsense-mediated mRNA decay(NMD)-related proteins,including NbUPF3 and NbSMG7,but not with NbUPF1.m^(6)Amodification-mediated restriction to PepMV infection is dependent on NMD-related *** findings provide new insights into the functionality of m^(6)Aanti-viral activity and reveal a distinct immune response that NMD factors recognize the m^(6)Areaders-viral m^(6)ARNA complex for viral RNA degradation to limit virus infection in plants.

关键词： m^(6)A Pepino mosaic virus plant defense m^(6)A readers NMD factors

Phomaketals A and B,pentacyclic meroterpenoids from a eupC overexpressed mutant strain of Phoma sp.

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Chinese Chemical Letters 2024年第7期35卷 350-354页

作者： Chuan Li Yangyang Han Yanan Zhai Ke Li Xingzhong Liu Zhuan Zhang Cai Jia Yongsheng Che NHC Key laboratory of biotechnology of Antibiotics Institute of Medicinal BiotechnologyChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100050China State Key laboratory of Toxicology&Medical Countermeasures Beijing Institute of Pharmacology&ToxicologyBeijing 100850China Department of Microbiology College of Life SciencesNankai UniversityTianjin 300071China

Phomaketals A(1)and B(2),two tropolonic meroterpenoids with the unprecedented pentacyclic skeletons,were isolated from the solid-substrate fermentation cultures of a eupC overexpressed mutant strain of the fungus Phoma sp.,together with a biogenetically related secondary metabolite pughiinin B(3),and the known one noreupenifeldin B(4).The structures of 1–3 were elucidated primarily by nuclear magnetic resonance(NMR)*** absolute configurations of 1 and 2 were assigned by electronic circular dichroism calculations and the calculated NMR with DP4+analysis,while that of 3 was established by single-crystal X-ray diffraction analysis using Cu Kα***,phomaketals A(1)and B(2)could be derived from the hypothetical tropolonic sesquiterpene intermediates neosetophomone B(6)and 9-R-neosetophomone B(6),respectively,via different reactions *** 1 showed antiproliferative effect only against the SUPB15 cells,with an 50%inhibitory concentration(IC50)value of 4.85μmol/L,while the co-isolated known meroterpenoid 4 displayed potent effects against three tumor cell lines,SUPB15,EL4,and H9,showing IC50values of 0.36–27.08μmol/L.

关键词： Phoma sp. Tropolonic sesquiterpenes Biosynthetic pathways Hetero-Diels–Alder Cytotoxicity

Single-cell transcriptomes provide insights into expansion of glial cells in Bombyx mori

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Insect Science 2024年第4期31卷 1041-1054页

作者： Xiaojing Liu Zhongjie Zhang Bo Hu Kai Chen Ye Yu Hui Xiang Anjiang Tan Jiangsu Key laboratory of Sericultural Biology and biotechnology School of BiotechnologyJiangsu University of Science and TechnologyZhenjiangJiangsu ProvinceChina Key laboratory of Silkworm and Mulberry Genetic Improvement Ministry of Agriculture and Rural AffairsThe Sericultural Research InstituteChinese Academy of Agricultural SciencesZhenjiangJiangsu ProvinceChina Guangzhou Key laboratory of Insect Development Regulation and Application Research Institute of Insect Science and Technology and School of Life SciencesSouth China Normal UniversityGuangzhouChina

The diversity of cell types in the brain and how these change during different developmental stages,remains largely *** life cycle of insects is short and goes through 4 distinct stages including embryonic,larval,pupal,and adult *** postembryonic life,the larval brain transforms into a mature adult version after *** silkworm,Bombyx mori,is a lepidopteran model ***,we characterized the brain cell repertoire of larval and adult *** by obtaining 50708 single-cell *** and 12 cell clusters from larval and adult brains were assigned based on marker genes,*** cell types include Kenyon cells,optic lobe cells,monoaminergic neurons,surface glia,and astrocyte *** further assessed the cell type compositions of larval and adult *** found that the transition from larva to adult resulted in great expansion of glial *** glial cell accounted for 49.8%of adult midbrain *** to flies and ants,the mushroom body kenyon cell is insufficient in ***,which accounts for 5.4%and 3.6%in larval and adult brains,*** of neuropeptide expression showed that the abundance and specificity of expression varied among individual ***,we found that ion transport peptide was specifically expressed in glial cells of larval and adult *** cell atlas dataset provides an important resource to explore cell diversity,neural circuits and genetic profiles.

关键词： brain neuropeptide silkworm single-cell transcriptome

CRISPR/Cpf1–FOKI-induced gene editing in Gluconobacter oxydans

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Synthetic and Systems biotechnology 2024年第2期9卷 369-379页

作者： Xuyang Wang Dong Li Zhijie Qin Jian Chen Jingwen Zhou Engineering Research Center of Ministry of Education on Food Synthetic biotechnology Jiangnan University1800 Lihu RoadWuxiJiangsu214122China Science Center for Future Foods Jiangnan University1800 Lihu RoadWuxiJiangsu214122China Key laboratory of Industrial biotechnology Ministry of Education and School of BiotechnologyJiangnan University1800 Lihu RoadWuxiJiangsu214122China Jiangsu Province Engineering Research Center of Food Synthetic biotechnology Jiangnan UniversityWuxi214122China

Gluconobacter oxydans is an important Gram-negative industrial microorganism that produces vitamin C and other products due to its efficient membrane-bound dehydrogenase *** incomplete oxidation system has many crucial industrial ***,it also leads to slow growth and low biomass,requiring further metabolic modification for balancing the cell growth and incomplete oxidation *** a non-model strain,*** lacks efficient genome editing tools and cannot perform rapid multi-gene editing and complex metabolic network *** the last 15 years,our laboratory attempted to deploy multiple CRISPR/Cas systems in different *** strains and found none of them as *** this study,Cpf1-based or dCpf1-based CRISPRi was constructed to explore the targeted binding ability of Cpf1,while Cpf1–FokI was deployed to study its nuclease activity.A study on Cpf1 found that the CRISPR/Cpf1 system could locate the target genes in *** but lacked the nuclease cleavage ***,the CRISPR/Cpf1–FokI system based on FokI nuclease was ***-gene knockout with efficiency up to 100%and double-gene iterative editing were achieved in *** this system,AcrVA6,the anti-CRISPR protein of *** was discovered for the first time,and efficient genome editing was realized.

关键词： Cell growth CRISPRi Anti-CRISPR protein Multi-gene editing Vitamin C

Regulation of 2-acetyl-1-pyrroline and grain quality in early-season indica fragrant rice by nitrogen and silicon fertilization under different plantation methods

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Journal of Integrative Agriculture 2024年第2期23卷 511-535页

作者： Yongjian Chen Lan Dai Siren Cheng Yong Ren Huizi Deng Xinyi Wang Yuzhan Li Xiangru Tang Zaiman Wang Zhaowen Mo State Key laboratory for Conservation and Utilization of Subtropical Agro-Bioresources College of AgricultureSouth China Agricultural UniversityGuangzhou 510642China Guangxi Key laboratory of Agricultural resources Chemistry and biotechnology Yulin Normal UniversityYulin 537000China Key laboratory for Conservation and Utilization of Subtropical Bio-resources Education Department of Guangxi Zhuang Autonomous Region/College of Biology and PharmacyYulin Normal UniversityYulin 537000China Scientific Observing and Experimental Station of Crop Cultivation in South China Ministry of Agriculture and Rural AffairsGuangzhou 510642China Guangzhou Key laboratory for Science and Technology of Fragrant Rice Guangzhou 510642China Key laboratory of Key Technology for South Agricultural Machine and Equipment Ministry of Education/College of EngineeringSouth China Agricultural UniversityGuangzhou 510642China

Fragrant rice has a high market value,and it is a popular rice type among consumers owing to its pleasant *** methods,nitrogen(N)fertilizers,and silicon(Si)fertilizers can affect the grain yield and fragrance of fragrant ***,the core commercial rice production attributes,namely the head rice yield(HRY)and 2-acetyl-1-pyrroline(2-AP)content of fragrant rice,under various nitrogen and silicon(N-Si)fertilization levels and different plantation methods remain *** field experiment in this study was performed in the early seasons of 2018 and 2019 with two popular indica fragrant rice cultivars(Yuxiangyouzhan and Xiangyaxiangzhan).They were grown under six N-Si fertilization treatments(combinations of two levels of Si fertilizer,0 kg Si ha^(−1)(Si0)and 150 kg Si ha^(−1)(Si1),and three levels of N fertilizer,0 kg N ha^(−1)(N0),150 kg N ha^(−1)(N1),and 220 kg N ha^(−1)(N2))and three plantation methods(artificial transplanting(AT),mechanical transplanting(MT),and mechanical direct-seeding(MD)).The results showed that the N-Si fertilization treatments and all the plantation methods significantly affected the HRY and 2-AP content and related parameters of the two different fragrant rice *** with the Si0N0 treatment,the N-Si fertilization treatments resulted in higher HRY and 2-AP *** rates of brown rice,milled rice,head rice,and chalky rice of the fragrant rice also improved with the N-Si fertilization *** N-Si fertilization treatments increased the activities of N metabolism enzymes and the accumulation of N and Si in various parts of the fragrant rice,and affected their antioxidant response *** key parameters for the HRY and 2-AP content were assessed by redundancy ***,the structural equation model revealed that the Si and N accumulation levels indirectly affected the HRY by affecting the N metabolism enzyme activity,N use efficiency,and grain quality of fragrant ***,high N

关键词： fragrant rice 2-AP content head rice yield mechanical planting nitrogen silicon

Enhanced extracellular production of alpha-lactalbumin from Bacillus subtilis through signal peptide and promoter screening

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Food Science and Human Wellness 2024年第4期13卷 2310-2316页

作者： Yuqi Zhu Pengdong Sun Chunjian Li Yu Zhang Yu Wang Jingyuan Li Yanfeng Liu Jian Chen Yang Deng College of Food Science and Engineering Qingdao Agricultural UniversityQingdao 266109China Key laboratory of Special Food Processing(Co-construction by Ministry and Province) Ministry of Agriculture Rural AffairsQingdao Agricultural UniversityQingdao 266109China Qingdao Special Food Research Institute Qingdao 266109China Science Center for Future Foods Engineering Research Center of Ministry of Education on Food Synthetic BiotechnologyJiangsu Province Engineering Research Center of Food Synthetic BiotechnologyJiangnan UniversityWuxi 214122China Key laboratory of Carbohydrate Chemistry and biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122China

Alpha-lactalbumin(α-LA)is a major whey protein found in breast milk and plays a crucial role in the growth and development of *** this study,Bacillus subtilis RIK1285 harboring AprE signal peptide(SP)was selected as the original strain for the production ofα-*** was found thatα-LA was identified in the pellet after ultrasonic disruption and centrifugation instead of in the fermentation *** original strain most likely only producedα-LA intracellular,but not *** improve the expression and secretion ofα-LA in RIK1285,a library of 173 homologous SPs from the *** 168 genome was fused with target LALBA gene in the pBE-S vector and expressed extracellularly in *** YjcN was determined to be the best signal *** in supernatant were observed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and purified by nickel column to calculate the highest yield signal *** addition,different promoters(P_(aprE),P_(43),and P_(glv))were compared and *** results indicated that the strain RIK1285-pBE-P_(glv)-YjcN-LALBA had the highestα-LA yield,reaching 122.04μg/*** study demonstrates successful expression and secretion of humanα-LA in *** and establishes a foundation for simulating breast milk for infant formulas and developing bioengineered milk.

关键词： Bacillus subtilis Alpha-lactalbumin Bioengineering milk Signal peptide Promoter screening

Chemical Synthesis of a Key Precursor Relevant to the Tetrasaccharide Repeating Unit from Treponema medium ATCC 700293

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Chinese Journal of Chemistry 2024年第14期42卷 1615-1622页

作者： Wenbin Sun Guangzong Tian Meiru Ding Chunjun Qin Xiaopeng Zou Jing Hu Jian Yin Key laboratory of Carbohydrate Chemistry and biotechnology Ministry of EducationSchool of Biotechnology&School of Life Sciences and Health EngineeringJiangnan UniversityLihu Avenue 1800WuxiJiangsu214122 China Wuxi School of Medicine Jiangnan UniversityLihu Avenue 1800WuxiJiangsu214122 China

Treponema is a Gram-negative anaerobic bacterium,among which the pathogenic Treponema can cause various diseases,such as venereal syphilis(Treponema pallidum),yaws(Treponema carateum),and oral diseases(Treponema denticola and Treponema medium).Although different from conventional lipopolysaccharides,the extracellular glycoconjugate of Treponema may still be a potential antigen and provide a candidate for vaccine ***,we completed the first chemical synthesis of Treponema medium ATCC 700293 tetrasaccharide precursor containing L-ornithine(L-Orn)and D-aspartic acid(D-Asp)*** efficiency of non-reducing end disaccharide formation has been improved by optimizing the assembly of the protecting groups in the donors and ***[3+1]glycosylation strategy attempted to reduce the length of the acceptor to increase the nucleophilicity of the hydroxyl group,thereby improving the efficiency of synthesizing the target *** L-Orn derivative was introduced at the final stage due to its influence on the glycosylation stereospecificity and ***,the successful introduction of two amino acid derivatives and the synthesis of a tetrasaccharide precursor with complex functional-group modifications have provided valuable insights for synthesizing other complex bacterial glycans.

关键词： Glycosylation Total synthesis Treponema medium ATCC 700293 Tetrasaccharide precursor Protecting group Oligosaccharides Regioselectivity Neighboring-group effects